2.Research advances of small-diameter vascular grafts.
Chao-jun TANG ; Gui-xue WANG ; Dang-heng WEI ; Xiao-yan DENG
Chinese Journal of Medical Instrumentation 2005;29(6):439-442
This review is a summary of some useful methods and advances about improving clinical applications to small-diameter vascular grafts in recent years, and it points out the developing orientation of small-diameter vascular grafts in the future.
Bioartificial Organs
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Endothelial Cells
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Endothelium, Vascular
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Tissue Engineering
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Vascular Grafting
3.Preventive effect of TGF-?_1 neutralizing antibody on flexor tendon adhesion from operation
Jin-Tang WANG ; Yin-Gang ZHANG ; Jun LI ; Nai-Gui BI ; Xue-Zhe HAN ; Xiao-Wei ZHANG ;
Chinese Journal of Microsurgery 2006;0(05):-
Objective To investigate the preventive effect of TGF-?_1 neutralizing antibody on flexor tendon adhesion from operation.Methods One hundred and eight leghon cocks performed anastomonsis op- eration were divieded into three groups randomly,as normal saline(control group),5?g/ml group,10?g/ml group of TGF-?_1,antibody.At 1 st,3rd,8th and 12th weeks respectively after operation,the flexor biomechan- ics test,HE staining,Masson staining,Sirius red-polarization staining and TGF-?_1 immunohistochemistry stai- ning were used.Results The max of strength of tendon and the stimulate active flexor from the experiment groups(5?g/ml group,10?g/ml) are higher than from the control group,The max of strength of tendon of the experiment groups are less at 8th weeks,and no difference at 12th weeks from the control group;Compared with the control group,the 10?g/ml group were less shorten the progress of inflammation and accelerated the progress of molding;In the experiment groups(5?g/ml group,10?g/ml),the density of the collagenⅠtype were less,the ratio ofⅠ/Ⅲcollagen and expression of the TGF-?_1 were decreasing.Condusion The study showed that applying of TGF-?_1 muhiclonal neutralizing antibody can inhibit efficiently the function of the TGF-?_1 during the flexor tendon repair,reduce tendon adhesion and scar fromation,however has no affec- tion of tendon intensity,suggesting it is a latent and efficient method for preventiong flexor tendon from adhe- ring after operation.
4.Effect of acute hypervolemic hemodilution on pharmacokinetics of propofol in patients undergoing total hip replacement
Jian-Guo TANG ; Gui-Long WU ; Li-Chao PENG ; Biao ZHU ; Jing CANG ; Changhong LIAO ; Zhanggang XUE
Chinese Journal of Anesthesiology 1994;0(01):-
Objective To investigate the influence of acute hypervolemic hemodilution(HHD)on pharmacokinetics of propofol.Methods Sixteen ASA Ⅰ or Ⅱ patients aged 20-55 yrs undergoing elective surgery under general anesthesia combined with epidural analgesia were randomly allocated into 2 groups(n=8 each);Ⅰ control group and Ⅱ HHD group.The patients were premedicated with intramuscular phenobarbital 0.1 g and scopolamine 0.3 mg.Right internal jugular vein was cannulated for CVP monitoring and blood sampling.Radial artery was cannulated for BP monitoring.All patients in both groups received lactated Ringer's solution(0.7 ml?kg~(-1)? number of hours of fasting before operation)before induction of general anesthesia.In HHD group 4% gelofusine 20 ml?kg~(-1) was infused at the rate of 20 ml?kg~(-1)?h~(-1).Anesthesia was induced with midazolam 0.04 mg?kg~(-1),fentanyl 4 ?g?kg~(-1) and propofol 1.5 mg?kg~(-1).Tracheal intubation was facilitated by succinylcholine 2 mg?kg~(-1).Anesthesia was maintained with isoflurane,fentanyl,vecuronium and epidural analgesia.ECG,BP, SpO_2,P_(ET)CO_2 and CVP were continuously monitored.Blood samples were taken at 1,2,4,6,10,15,30,45, 60,75,90,120,150,180,240,300 and 360 min after propofol was given Ⅳ for determination of plasma concentration of propofol(HPLC).Pharmacokinetic data were analyzed by 3P97 pharmacokinetic software.Results The two groups were comparable with respect to demographic data.Blood propofol concentrations were significantly lower in HHD group than in control group at 1,2,4,6,10 min after propofol injection(P<0.01), thereafter there was no significant difference in plasma propofol concentration between the two groups(P>0.05). The pharmacokinetic profile of propofol was well described by a standard three-compartment model.In HHD group V_C was significantly increased,K_(10) and Cl were significantly decreased and T_(1/2?) was significantly prolonged as compared with control group.Conclusion Acute HHD increases V_C,prolongs the T_(1/2?) and decreases K_(10) and Cl, suggesting that the effect of propofol may be potentiated by acute HHD.
5.Clinical trial of rectocele repair with longitudinal incision and transverse suture on the vaginal posterior wall.
Xue-gui TANG ; Zhi-jiu WU ; Li-juan DU
Chinese Journal of Gastrointestinal Surgery 2006;9(4):311-313
OBJECTIVETo investigate the clinical efficacy of rectocele repair with longitudinal incision and transverse suture on the vaginal posterior wall.
METHODSOne hundred and forty-six patients with rectocele were enrolled in our study from August 1999 to August 2003. The patients were randomly divided into two groups, and received traditional repair with longitudinal incision and longitudinal suture (control group, n=74) or repair with longitudinal incision and transverse suture on the vaginal posterior wall (study group, n=72). The efficacy and complications were compared between the two groups.
RESULTSIn the study group,only one case (1.4%) had no effect, and the total effective rate was 98.7%. The mean course of treatment was (11.0+/- 1.9) days. Only two cases (2.7%) had postoperative complication. In the control group, 8 cases (11.1%) had no effect, and the total effective rate was 88.9%. The mean course of treatment was (17.4+/- 1.6) days. Twenty-nine cases (40.3%) had postoperative complications. There were significant differences in the efficacy and complications between the two groups (both P< 0.01).
CONCLUSIONThe refined rectocele repair with longitudinal incision and transverse suture on the vaginal posterior wall has good efficacy with shorter curative period and less complications.
Adult ; Aged ; Female ; Humans ; Middle Aged ; Rectocele ; surgery ; Sutures ; Vagina ; surgery
6.Concentration polarization of low density lipoprotein at the distal end of carotid stenosis promotes atherogenesis.
Dang-Heng WEI ; Gui-Xue WANG ; Chao-Jun TANG ; Lin-Qi YE ; Li YANG ; Ling-Hong DENG ; Lu-Shan LIU ; Zuo WANG ; Chao-Ke TANG
Acta Physiologica Sinica 2007;59(6):831-839
To test the hypothesis that concentration polarization of atherogenic lipids may occur in the arterial system and play an important role in localization of atherosclerosis, we simulated and measured in vitro the luminal surface concentration of low density lipoprotein (LDL) in local stenosis at the distal end of carotid artery by number simulation and laser scanning confocal microscopy, then we designed carotid stenosis model to test the role of LDL concentration polarization in atherogenesis. The in vitro experiment showed that the luminal surface LDL concentration was higher than the bulk concentration as predicted by the concentration polarization theory. The relative luminal surface LDL concentration changed with the flow velocity and ratio of stenosis. The wall concentration of LDL was highest in the round tube with 40% stenosis at the same velocity, while the wall concentration of LDL was higher when Re was 250 than Re was 500 at the same extent of narrowness. The animal experiment also revealed that general atherogenic plaques obviously occurred at the distal end of local stenosis where concentration polarized. The results strongly support our hypothesis that concentration polarization of lipoproteins occurs in local stenosis at the distal end of carotid artery, and this in turn promotes the localization of atherosclerosis which develops in the arterial system.
Animals
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Atherosclerosis
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physiopathology
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Carotid Stenosis
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physiopathology
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Disease Models, Animal
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Lipoproteins, LDL
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metabolism
7.Effect of interleukin-10 on the phenotype and function of cultured human dendritic cells.
Tong ZHOU ; Gui-zhi SUN ; Yu-mei ZHANG ; Yan-yun ZHANG ; Dong-qing ZHANG ; Xue-ming TANG ; Nan CHEN
Chinese Medical Journal 2005;118(15):1299-1302
Cell Survival
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drug effects
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Cells, Cultured
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Dendritic Cells
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drug effects
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immunology
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physiology
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Humans
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Immunophenotyping
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Interleukin-10
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pharmacology
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Interleukin-12
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genetics
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secretion
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Interleukin-12 Subunit p35
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Interleukin-12 Subunit p40
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Protein Subunits
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genetics
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secretion
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RNA, Messenger
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analysis
8.Clinical Efficacy of Modified Zhishi Daozhiwan on Slow Transit Constipation
Fang LIU ; Xian-peng WEI ; Xue-gui TANG
Chinese Journal of Experimental Traditional Medical Formulae 2020;26(2):92-97
Objective::To observe the clinical efficacy of modified Zhishi Daozhiwan on slow transit constipation (STC) and the effect on gastrointestinal hormones and intestinal flora. Method::One hundred and sixty patients were randomly divided into control group and observation group by random number table. Patients in control group got Maren Wan, 1-2 pills/time, 2 times/days, and mosapride citrate tablets, 1 piece/time, 3 times/days. Patients in observation group got modified Zhishi Daozhiwan, 1 dose/day. A course of treatment was 4 weeks. Before and after treatment, the main symptoms of constipation and patient assessment of constipation symptoms (PAC-SYM) were scored. And times of spontaneous complete bowel movement (SCBM) and colonic transmission test were recorded. And levels of intestinal flora and motilin (MTL), vasoactive intestinal peptide (VIP), substance P (SP) and gastrin (GAS) were detected. The 12-week follow-up of patients (SCBM≥3) was recorded, and the relapse of disease was calculated. Result::According to rank sum test analysis, the clinical efficacy in observation group was better than that in control group (
9. Clinical Efficacy of Taohua Tang and Buzhong Yiqi Tang in Treatment of Deficiency Syndrome of Crohn's Disease
Peng-fei KONG ; Xian-peng WEI ; Xue-gui TANG
Chinese Journal of Experimental Traditional Medical Formulae 2019;25(24):54-59
Objective:To observe clinical efficacy of Taohua Tang and Buzhong Yiqi Tang on Crohn's disease (CD) at active phase (deficiency-cold in spleen and stomach), in order to observed its effect on Th1 and Th17 cytokines. Method:According to random number table, 86 patients with CD were divided into control group (42 cases) and observation group (44 cases). The control group (mild) was given SASP, 3-4 g·d-1, Po, tid. The control group (moderate or poor efficacy of SASP) was given prednisone acetate, 0.75 mg·kg-1·d-1, Po, tid. Observation group was given Taohua Tang and Buzhong Yiqi Tang in addition to therapy of the control group, 1 dose·d-1. The course of treatment was 12 weeks. Before and after treatment, Best CDAI, SES-CD, IBDQ and deficiency syndrome were scored, and levels of CRP, ESR, ALB, HB, PLT, IFN-γ, TNF-α, IL-2 and IL-17 were measured before and after treatment. Result:After treatment, the effect of traditional Chinese medicine(TCM) syndromes in the observation group was better than that in the control group (Z=2.058, P<0.05). The clinical remission rate, the effective rate and the endoscopic remission rate in the observation group were 93.18%, 100% and 86.36%, which were higher than 76.19%, 83.33% and 66.67% in the control group (P<0.05). Best CDAI, SES-CD and IBDQ scores of the observation group were lower than those of the control group (P<0.01), while IBDQ score was higher than that of the control group (P<0.01). CD activity in the observation group was lower than that in the control group (Z=2.112, P<0.05). The degree of inflammation in the observation group was lighter than that in the control group (Z=2.288, P<0.05). CRP, ESR and PLT levels in the observation group were lower than those in the control group (P<0.01), whereas ALB and HB levels were higher than those in the control group (P<0.01). IFN-γ, TNF-α, IL-2 and IL-17 levels in the observation group were lower than those in the control group (P<0.01). Conclusion:In addition to the therapy of conventional western medicine, Taohua Tang and Buzhong Yiqi Tang in treatment of deficiency syndrome of Crohn's disease (CD) can control the activity degree of the disease, reduce the degree of illness and inflammation, and improve the remission rate and the quality of life, with a better clinical efficacy than the pure western medicine therapy.
10. Clinical Observation of Addition and Subtraction Therapy of Danggui Shaoyaosan Combined with Huaihuasan to Ulcerative Colitis with Syndrome of Dampness-heat in Large Intestine During Active Stage
Fang LIU ; Na LEI ; Xue-gui TANG
Chinese Journal of Experimental Traditional Medical Formulae 2019;25(20):82-87
Objective:To observe the effects of addition and subtraction therapy of Danggui Shaoyaosan combined with Huaihuasan on ulcerative colitis (UC) with syndrome of dampness-heat in large intestine during active stage, and the effects on brain-gut petide neurotransmitter and inflammatory cytokines. Method:A total of 130 cases were included and randomly divided into control group and observation group, 65 cases in each group. In the control group, the patients received oral administration of mesalazine enteric-coated tablets, 1.0 g/time and 3 times/days. Severe patients received prednisone acetate tablets (0.75 mg·kg-1·d-1) in several times by oral administration. Based on the treatment in control group, patients in observation group also received addition and subtraction therapy of Danggui Shaoyaosan combined with Huaihuasan, 1 dose/day. Both groups were treated for 4 weeks. Symptom scores, Mayo scores, colonic mucosa scores and Inflammatory Bowel Disease Questionnaire (IBDQ) scores were assessed before and after treatment. Patients in remission stage were followed up for 6 months to record the recurrence. Before and after treatment, vasoactive intestinal peptide (VIP), substance P (SP), somatostatin (SS), interleukin-1 (IL-1), IL-6, IL-4 and IL-10 were detected. Result:After 4 weeks of treatment, the clinical remission rate was 93.22%in the observation group, better than 80.7%in the control group (χ2=4.035,P<0.05). The scores of various symptoms in the observation group were lower than those in the control group (P<0.01). The Mayo scores and colonic mucosal scores in the observation group were lower than those in the control group (P<0.01). The IBDQ scores in all dimensions and the total IBDQ scores in the observation group were higher than those in the control group (P<0.01). In 6 months of follow up for the patients in clinical remission stage, the recurrence rate in the observation group was 18.18%, lower than 36.96%in the control group (χ2=4.509,P<0.05), and the recurrence time was longer than that in the control group (P<0.01). The levels of serum IL-1 and IL-6 in the observation group were lower than those in the control group (P<0.01), and the levels of IL-4 and IL-10 were higher than those in the control group (P<0.01). Plasma VIP and SS levels in the observation group were higher than those in the control group (P<0.01), while SP levels were lower than those in the control group (P<0.01). Conclusion:On the basis of conventional western medicine treatment, addition and subtraction therapy of Danggui Shaoyaosan combined with Huaihuasan in the treatment of UC (dampness-heat in large intestine) during active stage can control the disease activity in a short term, promote restoration of the colonic mucosa. And delay the recurrence in a long term, reduce the recurrence rate, regulate ghrelin neurotransmitters and pro-and anti-inflammatory cytokines levels.