Objective To study the genetic polymorphism of 10 Y-chromosome STR loci by the allelic frequencies and haplotype frequencies in a Mongolia population sample of Baotou city.Methods We used PCR and polyacrylamidedel electrophoresis(PAGE) following silver staining to analyze the blood sample from the 99 unrelated Mongolia male individuals.Results The allele diversity values for each locus ranged from 0.458(DYS462) to 0.727(DYS389Ⅰ,DYS389Ⅱ) and gene diversity was above 0.5 except DYS462(0.458) in DYS392,DYS438,DYS439,DYS456,DYS459,DYS460, DYS461,DYS462,DYS389Ⅰ and DYS389Ⅱ loci.Furthermore,98 haplotypes were found from 10 loci and haplotype diversity was 0.98.Conclusion The haplotypes of the 10 Y-STR loci have the higher genetic polymorphism.

Antioxidants ; therapeutic use ; Humans ; Infant, Newborn ; Oxygen Inhalation Therapy ; Persistent Fetal Circulation Syndrome ; drug therapy ; metabolism ; Reactive Oxygen Species ; metabolism

The rapid development of minimally invasive surgery in thoracic surgery has significant advantages over traditional surgery.At the same time, problems such as the difficulty in obtaining specimens from small incisions and the increase in operating costs due to special medical consumables have also emerged. Therefore, how to remove surgical specimens safely, efficiently and cost-effectively is an important issue faced by thoracic surgeons during surgery. Operating room sterile gloves are often used as specimen collection bags and widely used in thoracic surgery and many other departments because of their easy availability, low cost, and good flexibility. At present, most of the glove specimen collection bags are based on sterile gloves, and various manufacturing methods have evolved by the combination with instruments and sutures.This article will briefly review the application of various forms of glove specimen retrieval bags in thoracoscopic surgery reported in existing research, analyze their advantages, disadvantages and scope of application, so as to provide references for future clinical applications and technological innovation researches.

Objective To investigate the surgical treatment of Maisonneuve injury associated with posterior malleolus fracture under direct visualization via the posterolateral approach.Methods Between January 2008 and June 2015,27 patients with Maisonneuve injury associated with posterior malleolus fracture were treated at our department.They were 18 men and 9 women,aged from 20 to 60 years (average,40.1 years).All fractures and syndesmoses received open reduction under direct visualization and internal fixation via the posterolateral approach.The posterior malleolus fractures were fixed with screws or plate and the syndesmoses fixed using cortical bone screws.Functional results were evaluated using the Baird-Jackson ankle scoring system.Results The patients were followed up for 8 to 30 months (average,18 months).No wound infection,necrosis,dehiscence,lose of reduction or implant failure was found during follow-up visits.All cases got bony union at the last visit.The excellent and good rate was 92.6％.Conclusion Since the posterolateral approach allows good access to the Maisonneuve injury and posterior malleolus fracture under direct visualization,it facilitates accurate direct fracture reduction,stable fixation and early exercise.

OBJECTIVE To determine the bacterial distribution and resistance of biliary tract infections in China.METHODS Disc diffusion test,MIC test or E-test were used to detect the antimicrobial sensitivity of bacterial isolates from bile specimens.WHONET software was applied for analysis of the bacterial sensitive data from 84 tertiary Mohnarin member hospitals at different area in China from June 1,2006 to May 31,2007.RESULTS A total of 1441 bacterial strains were collected in the survey period,which included 952 Gram-negative strains(66.1%),of which the top three were Escherichia coli(31.0%),Klebsiella pneumoniae(8.4%) and Pseudomonas aeruginosa(7.8%) and 489 Gram-positive strains(33.9%),the top three were Enterococcus faecalis(11.6%),E.faecium(9.5%) and Staphylococcus(7.4 %).Susceptibility results showed that the resistant rates of E.coli and K.pneumoniae to three generation cephalosporins and quinolones were 25.9-70.0%,17.9-44.8% and 66.9-70.9%,34.6-40.0%,respectively,which were comparable with the results of overall bacteria collection.The resistant rates of P.aeruginosa to imipenem and cefoperazone/sulbactam were 37.5%,13.8%,and the detection rate of meticillin-resistant Staphylococcus aureus(MRSA) from bile samples was higher than the results of overall bacteria collection.Vancomycin-resistant Enterococcus(VRE) were rare.CONCLUSIONS Gram-negative bacilli are still the predominant pathogens in biliary tract infections;the overall bacterial resistance is severe.MRSA is increasing.Prudent application of antibiotics in biliary tract infections needs to be emphasized.

Recently,the detection of circulating tumor cells(CTCs)in peripheral blood is used in clinical practice as a form of‘ liquid biopsy’. The self-seeding mechanism of CTCs provides a novel approach to explore the growing mechanism of malignant tumor and developing corresponding targeting therapies. A decade ago,the CellSearch system, which can capture and enumerate CTCs,has been validated by Food and Drug Administration( FDA)as an aid for monitoring the relapse of tumor after radical operation in patients with breast,prostate and colorectal cancer. In recent years,although the separation and detection technique of CTCs has been promoted significantly,the clinical significance of CTCs in tumors of digestive system is still under investigation. This article reviewed the research progress of peripheral blood CTCs in digestive system tumors.

Objective:To identify the factors that affect the duration of forced eruption of the impacted maxillary canines.Methods:Data of 46 successfully treated impacted maxillary canines with forced eruption were included.The relation of duration of forced eruption and the age of the patients(y),the distance between the impacted maxillary canine cusp tip and the occlusal plane(d),the angle be-tween the axis of the impacted maxillary canine and the occlusal plane(α),mesiodistal position of the canine cusp tip in relation to the adjacent teeth(ZB),the angle between the canine axis and the lateral incisor axis(β)was analyzed.Results:The results indicated a direct correlation of the duration of forced eruption with the patient age and d value,the more mesial location of the canine cusp tip to the midline,the longer treatment time was needed.In contrast,there was an inverse correlation between the duration of forced eruption and αvalue.Conclusion:The patient's age and the position of the impacted maxillary canines are the important predictors of the dura-tion of the forced eruption.

BACKGROUND:Percutaneous vertebroplasty with bone cement injection for osteoporotic vertebral compression fractures can quickly relieve pain and increase vertebral strength, but vertebral re-fractures often occur because of osteoporosis. OBJECTIVE:To investigate the therapeutic effect of percutaneous vertebroplasty with bone cement injection combined with zoledronic acid in the treatment of osteoporotic vertebral compression fractures. METHODS:Six patients with osteoporotic vertebral compression fractures were enrol ed, including two males and four females, aged 60-86 years. These patients with T12-L4 vertebral fractures underwent percutaneous vertebroplasty with polymethyl methacrylate acrylate bone cement treatment. At 4 weeks after bone cement injection, al patients were administrated with intravenous injection of zoledronic acid (100 mL:5 mg) as anti-osteoporosis treatment, 30 drops per minute, once a year, for consecutive 2 years. The 2-year periodic imaging was performed to observe the clinical efficacy on vertebral compression fractures, bone density and adjacent vertebral fractures. RESULTS AND CONCLUSION:At 48 hours after bone cement injection, pain symptoms were relieved in the six cases. At the end of 2-year fol ow-up, fractures healed in al the six cases without delayed union or nonunion, and there was no bone cement leakage, nerve root compression, dural involvement, needle opening or wound infection and pulmonary embolism;the bone density was significantly higher than that before treatment (P<0.05), and no adjacent vertebral fractures occurred. These findings indicate that the percutaneous vertebroplasty with bone cement injection combined with zoledronic acid can significantly relieve pain, improve vertebral stability and bone density, and reduce the incidence of adjacent vertebral fractures in patients with osteoporotic vertebral compression fractures.

ObjectiveTo observe the effects of mechanical ventilation with different tidal volumes (VT) on coagulability and fibrinolytic characteristics in rabbits with acute respiratory distress syndrome (ARDS) induced by two-hits with oleic acid (OA) and lipopolysaccharide (LPS).Methods Forty healthy adult male rabbits were randomly divided into five groups (8 rabbits in each group): sham operation group, model group, low VT group (6 mL/kg), rontine VT group (10 mL/kg), high VT group (15 mL/kg). ARDS model was reproduced by sequential injection of 0.1 mL/kg OA and 500μg/kg LPS via auricular vein, and the rabbits in sham operation group received normal saline in same volume. Mechanical ventilation was performed in different VT groups after model reproduction, and the end of the experiment was determined as 6 hours after LPS injection. Blood was collected from the carotid artery at 30 minutes and 360 minutes after LPS injection, with which arterial partial pressure of oxygen (PaO2) was determined, and oxygenation index was calculated. Internal jugular vein blood was collected at 5, 120, 240, and 360 minutes after LPS injection, and activated partial thromboplastin time (APTT), prothrombin time (PT), fibrinogen (Fib) and antithrombinⅢ(AT-Ⅲ) were determined. The blood was collected at the end of the experiment, and then the rabbits were sacrificed, and serum levels of procollagen typeⅢ (PⅢP), plasminogen activator inhibitor-1 (PAI-1) as well as PⅢP level in lung tissue were determined. The wet/dry weight ratio (W/D) of lung tissues was calculated.Results Compared with sham operation group, oxygenation index at both 30 minutes and 360 minutes were significantly decreased in model group, while W/D ratio was significantly increased. PT and APTT at 5 minutes were significantly shortened followed by a gradual increase. Fib and AT-Ⅲ showed no change at 5 minutes followed by a gradual decrease, while serum PAI-1 and PⅢP as well as PⅢP in lung tissue were significantly increased. There was no significant difference in oxygenation index between all VT groups and model group, with a tendency to increase in low VT group. W/D ratio in low VT group was the lowest (5.09±0.19), and it was significantly lower than that of the model group (6.02±0.27, P< 0.01), while it was the highest in high VT group (6.27±0.32). PT and APTT in all VT groups were gradually prolonged, and Fib and AT-Ⅲ were gradually decreased. PT and APTT in low VT group were significantly shorter than those in model group from 120 minutes on [PT (s): 120 minutes: 8.90±0.28 vs. 11.43±0.28, 240 minutes: 9.18±0.21 vs. 11.99±0.50, 360 minutes: 9.25±0.15 vs. 12.49±0.29; APTT (s): 120 minutes: 69.09±3.91 vs. 76.08±4.21, 240 minutes: 67.53±2.14 vs. 79.71±2.25, 360 minutes: 66.95±1.13 vs. 83.21±4.01, allP< 0.05], Fib (g/L) was significantly elevated (120 minutes: 3.80±0.09 vs. 3.38±0.15, 240 minutes: 3.91±0.05 vs. 2.47±0.16, 360 minutes:4.06±0.13 vs. 2.39±0.16, allP< 0.05), and no significant difference was found in AT-Ⅲ. Serum contents of PAI-1 and PⅢP as well as PⅢP in lung tissue were significantly lowered [serum PAI-1 (ng/L): 1.14±0.26 vs. 1.63±0.91, serum PⅢP (ng/L): 1.62±0.52 vs. 2.91±0.64, lung PⅢP (ng/L): 4.40±0.58 vs. 5.75±0.47, allP< 0.01]. The change tendency of all parameters in routine VT group was lower than that in low VT group, PT at 120 minutes and 360 minutes, APTT at 240 minutes and 360 minutes was significantly shorter than that in model group, and Fib at 120 minutes and 240 minutes were significantly higher than those in model group (allP< 0.05). No significant difference was found in AT-Ⅲ, serum PAI-1 and PⅢP as well as lung PⅢP as compared with model group. PT and APTT at 360 minutes in high VT group were significantly longer than those in model group, Fib at 360 minutes was significantly lower than that in model group, and lung PⅢP was significantly higher than that in model group. Conclusions There are some important changes in coagulability which changes from a hyper state into a hypo coagulate state, while fibrinolysis is inhibited during the pathological process of ARDS produced by two-hit of OA and LPS. Mechanical ventilation with low VT can obviously improve coagulability and fibrinolytic status, while ventilation with routine VT has little effect on coagulability and fibrinolytic status. Mechanical ventilation with high VT, however, will greatly deteriorate the coagulability and fibrinolytic function in ARDS.

Objective To explore the change rule of serum thyroid hormone level in senile patients. Methods Totally 2433 elderly patients in our hospital during January to August 2011 were divided into three groups by age:adult group aged(46.0± 10.8)years(n=1030),elderly group aged (69.5±6.3)years (n=848) and advanced age group aged (83.9±3.8)years (n=555).The levels of serum free triodothyronine (FT3),free thyroxine (FT4),thyroid-stimulating hormone (TSH) were determined by chemistry luminescence technique. Results The levels of serum FT3 [(4.33 ± 1.22)pmol/L,(3.96 ± 0.89) pmol/L vs.(5.08 ± 2.99) pmol/L,t=7.075,8.799,both P=0.000] and FT4 [(18.32±5.81)pmol/L,(18.58±6.71)pmol/L vs.(19.87±10.97) pmol/L,t=3.732,2.709,both P=0.000] were lower in the elderly and advanced age than in adult.The levels of serum TSH were higher in the elderly than in adult (P=0.002),while no difference was found between advanced age and adult (P=0.134)[(5.51± 17.39) mU/L in the elderly,(3.33±5.65) mU/L in adults vs.(4.53±14.37)mU/L in advanced age].There were no significant differences in the levels of serum FT3,FT4 and TSH between advanced age and the elderly (all P＞0.05).The detection rate of the decreased FT3,FT4 and TSH in elderly and advanced age were (13.3％ vs.25.8％),(2.9％vs.1.8％),(6.8％vs.12.3％),while the detection rate of increment were (3.1 ％vs.0.1％),(8.6％vs.9.9％),(18.0％vs.15.7％),respectively.The rates of declined FT3 and TSH were obviously higher in the advanced age than in the elderly (x2=39.96,15.83,all P =0.000),but the rates of declined FT4 and increased FT3 were lower in the advanced age than in the elderly (x2 =27.84,16.32,all P=0.000). Conclusions The abnormal detection rates of thyroid hormones are high in the elderly,thus due attention should be paid to thyroid function test for early diagnosis and treatment of senile thyroid disease.