Objective:To explore the effect of costunolide on sensitivity of chronic myeloid leukemia cell line K562/ADR to doxorubicin and its mechanism.Methods:K562/ADR cells in the logarithmic phase were used, and the cells were treated with different concentrations of costunolide, doxorubicin or costunolide combined with doxorubicin for 72 h. The cell proliferation was detected by CCK-8 method, the cell proliferation rate was calculated, and the half inhibitory concentration (IC 50) of the two drugs was obtained. The cells were treated with 10 μmol/L costunolide, 10 μmol/L doxorubicin or costunolide combined with doxorubicin for 48 h, the apoptotic rate was detected by flow cytometry, and the expression level of p38-MAPK pathway related proteins was detected by Western blot. Results:The cell proliferation rate in the costunolide combined with doxorubicin group was lower than that in the corresponding concentration of the two drugs alone groups, and the differences were statistically significant both ( P < 0.05). The IC 50 of doxorubicin for K562/ADR cells was (13.50±0.86) μmol/L, and costunolide was (7.30±0.55) μmol/L ( t = 7.044, P = 0.002). The results of flow cytometry showed that the apoptosis rate of K562/ADR cells in the 10 μmol/L costunolide combined with 10 μmol/L doxorubicin group was higher than that of the blank control group, costunolide alone group and doxorubicin alone group, and the differences were statistically significant [(19.68±3.21)% vs. (2.96±0.87)%, (9.34±2.89)%, (9.18±2.13)%, all P<0.01]. Compared with the 10 μmol/L costunolide alone group and the 10 μmol/L doxorubicin alone group, the expression of the apoptosis inhibitor protein bcl-2 in the two-drug combination group was down-regulated, and the expressions of bad, p-p38, cleaved-caspase-3 and cleaved-PARP proteins were up-regulated. Conclusion:Costunolide can enhance the inhibitory effect of doxorubicin on the proliferation of K562/ADR cells and promote doxorubicin-induced apoptosis, which may reverse the drug resistance of K562/ADR cells by regulating the p38-MAPK pathway.

Objective To investigate the protective effect of fructose 1,6-diphosphate (FDP) on the brain against ischemia-reperfusion (I/R) injury and the possible mechanism. Methods One hundred and eighty SD rats weighing 275-325 g were randomly divided into 3 groups (n = 60 each): Ⅰ gham operation group; Ⅱ I/R group and Ⅲ FDP group. Global cerebral I/R was produced by 4-vessel technique. Bilateral vertebral arteries were coagulated and bilateral common carotid arteries were occluded for 5 min and then released for reperfusion. In sham operation the four vessels were exposed but not occluded. In FDP group FDP 1.5 mg?kg-1 was given Ⅳ when reperfusion was started, while in sham-operation group and I/R group normal saline (NS) 1.5 ml?kg-1 was given Ⅳ instead of FDP. The animals were killed at 2, 6, 12, 24, 48 and 72 h of reperfusion ( n = 5 each) for determination of cerebral SOD activity and MDA contents, the number of apoptotic neurons (TUNEL) and expression of P38 and Ref-1 in the brain (immuno-histochemical method) .Results The MDA content was significantly higher whereas the SOD activity and P38 and Ref-1 expression were significantly lower at all time points in I/R group than in sham operation group ( P

Objective: To develop a quantitative analysis of multi-components by single-marker (QAMS) method for the simultaneous determination of six triterpene acid (euscaphic acid, tormentic acid, maslinic acid, corosolic acid, oleanolic acid, and ursolic acid) contents in the effective fraction of Eriobotryae Folium. Methods: Ursolic acid was used as the internal reference substance, and the relative correlation factors (RCF) of euscaphic acid, tormentic acid, maslinic acid, corosolic acid, and oleanolic acid were determined by HPLC-ELSD with good reproducibility. The contents of the five components were calculated according to the RCF, respectively. The contents of these six triterpene acids in 15 batches of effective fraction were determined by the external standard method. The rationality, feasibility, and repeatability of the QAMS method were verified by comparing the results obtained from the two different methods. Results: For the six triterpene acids, there was no significant difference between the quantitative results with the two different methods in the 15 batches. Conclusion: The method established in this research is accurate and feasible that it just needs to assay single-marker (ursolic acid) for the determination of six triterpene acids in the effective fraction of Eriobotryae Folium simultaneously. Therefore, this method could provide a new reference for the quality assess of multi components in Chinese materia medica.

Adult ; Calculi ; complications ; Foreign Bodies ; complications ; Humans ; Male ; Nasal Cavity ; injuries ; Nasal Septum ; injuries ; Palate, Hard ; injuries

Sepsis is a complex systemic inflammatory response syndrome caused by invasion of a varie-ty of pathogenic bacteria. The disease spectrum includes sepsis, severe sepsis, septic shock and multiple organ failure. Mitochondria are referred to as “power plants”, providing energy for viscera to maintain normal func-tion. Immune disorders,the activation and release of a series of inflammatory factors,anomalies of intracellular signal transmission and oxidative stress in sepsis can induce mitochondrial dysfunction. Recent research suggests that mitochondrial autophagy also plays a certain role in mitochondrial dysfunction. This article reviews the mechanism of mitochondrial functional damage during the pathogenesis of sepsis.

Objective To understand the status of intensive care unit-acquired lower respiratory tract infection (ICU-LRTI),and the distribution characteristics of pathogens,so as to provide the basis for taking preventive and control meas-ures,and scientific diagnosis and treatment for patients.Methods Targeted monitoring data on healthcare-associated infec-tion (HAI)in ICUs of 32 hospitals in a province in 2013 were investigated retrospectively.Results The incidence of ICU-LRTI was 5.79%,ventilator usage rate was 31.25%,incidence of ventilator-associated pneumonia(VAP)was 26.93‰;There was no linear correlation between ventilator usage rate and incidence of VAP(r=0.160,P=0.380).A total of 1 593 pathogens causing LRTI were detected,the major were gram-negative bacteria (75.77 %,n=1 207),followed by gram-positive bacteria(18.21%,n=290),fungi(5.90%,n=94),Mycoplasma pneumonia and other pathogens(0.12 %,n= 1 for each).The top five detected pathogens causing LRTI were Acinetobacter baumannii ,Pseudomonas aeruginosa ,Staph-ylococcus aureus ,Klebsiella pneumoniae and Escherichia coli ,accounting for 25.49%,15.26%,14.63%,13.37% and 5.09% respectively.Conclusion Targeted monitoring on ICU is helpful for realizing healthcare-associated LRTI, each hospital should conduct targeted monitor to find out the causes of HAI,as well as improve the awareness of VAP among ICU health care workers.

Objective To study the factors affecting the functional restoration of the hip joint with femoral intertrochanteric fractures.Method44 patients with femoral intertrochanteric fractures were treated from Aprail 2004 to June 2006.The function of the hip joint was evaluated with the Harris hip score. Seven factors including age,sex,preoperative ASA class,preoperative time,fracture type,therapeutic measures,time of functional exercise were analyzed statistically with x2 test and logistic multiple regression with a significant difference (P

[Objective]To explore the feasibility and effect of modified minimally invasive percutaneous pedicle screws osteosynthesis for the treatment of thoraco-lumbar vertebra compression fracture.[Method]Twelve cases of thoraco-lumbar fracture without neural impairment were enrolled who did not need laminotomy decompression from June 2005 to June 2007.With the help of C-arm image intensifier,the pedicle screws were inserted through four small longitudinal incisions(1-1.5 cm)and modified surgical instruments.Perioperative parameter,postoperative imaging index,visual analog scale(VAS)and Oswestry Disability Index(ODI)were compared with the treatment of conventional open pedicle screws osteosynthesis in other 18 cases.[Result]All cases were followed up from 8 to 25 months(mean 13.2 months).The operation time and length of stay in minimal invasive group were significantly shorter than that in conventional group,the amount of operative bleeding and drainage of postoperation decreased obviously(P

0.05). Incision size,operating time,blood loss,hospital stay,medical device expenses,postoperative VAS and ODI,clinical outcomes,fusion rates,and complication rates were studied and tested with independent sample t test and x2 test.[Results]The mean follow-up was 19.1 months (range,12~25 months) in group A and 24.6 months (range,9~36 months) in group B.There were statistically significant differences in the incision size,operating time,blood loss,hospital stay and medical device expenses (P0.05).[Conclusion]Unilateral transforaminal lumbar interbody fusion with unilateral pedicle screw fixation has the advantages of small incision,little bleeding,no destruction of the contralateral structure,short operating time or hospital stay,low medical device expenses and good recovery.But the operation indications must be strictly defined.

Objective To understand how resistance and whether its compensatory hyperinsulinemia will influence the ovulatory function and sex hormone level,especially in the testosterone level and blood lipid level and to observe the effects of metformin on the polycystic ovary syndrome with pseudoacanthosis nigricans and the insulin resistance.Methods The method of the research is to carry out a three-month tretment for 30 patients who were suffering from the polycystic ovary syndrome with the pseudoacanthosis nigricans and the insulin resistance.During the treatment much attention was drawn to the following aspects of the patients.Such as the body mass index,the waist hip ratio,the fasting blood glucose,the fasting insulin,two hours after postcibal blood glucose and postcibal insulin,the insulin sensitive index,the change of blood lipid and sex hormone level,and the skin lesion change.Results After the treatment,all the indexes fell except that the insulin sensitive index rose.There was significancely difference(P