Objective To evaluate the effect of one-visit endodontic treatment for infected root canals with combination of nickel-titanium instruments and ultrasonic technique and warm vertical technique.Methods According to treatment,90 teeth were randomly divided into experimental and control groups.Two groups were used nickel titarlium root canal preparation.Experimental group:51 teeth for ultrasonic root canal irrigation,and warm vertical technique was employed to fill the root canal.Control group:39 teeth for rinse syringes,and warm vertical technique was employed to fill the root canal.Washing fluid was 2％ sodium hypochlorite.The root canal filling was observed after a week and one year of postoperative pain response.Results A week later,two groups of post-operative pain showed that there was significant difference in responses (x2 =15.525,P ＜ 0.05).One year later,the two groups showed no significant difference in efficacy(x2 =0.085,P ＞ 0.05).Conclusion The combination of nickel-titanium instruments and ultrasonic irrigation technique and warm vertical obturation,for the treatment of infected root canals was a method to complete the root canal filling,could reduce both the number of patients subsquent visits and the postoperative pain response,while it could improve the quality of root canal filling.

BACKGROUND:At present, a great quantity of research has shown the effectiveness of traditional Chinese medicine and bone marrow mesenchymal stem cel s for vascular restenosis. However, studies concerning their combined application to restenosis after percutaneous transluminal angioplasty with diabetes mel itus are presently lacking.
OBJECTIVE:To observe the effects of combined application of bone marrow mesenchymal stem cel s and benefiting-Qi nourishing-Yin and dissolving-congestion prescription on restenosis after percutaneous transluminal angioplasty in dogs with diabetes mel itus.
METHODS:A dog model of vascular restenosis with diabetes mel itus was established by bal oon injury of femoral artery and intravenous injection of al oxan. After successful model induction, 22 dog models were randomly divided into three groups:model group (n=6), treatment with Chinese medicine (n=8), and combined treatment with bone marrow mesenchymal stem cel s and Chinese medicine (n=8). Serum vascular endothelial growth factor levels were measured using enzyme-linked immunosorbent assay preoperatively and at 1, 2, 4 and 8 weeks postoperation. Samples of vessels were taken to conduct pathomorphological observation and quantitative analysis of proliferation degree. Tissues, including heart, liver, kidney and pancreatic gland, were col ected to evaluate the safety of stem cel transplantation using hematoxylin-eosin staining at 8 weeks postoperation.
RESULTS AND CONCLUSION:Serum vascular endothelial growth factor levels began to increase at 1 week postoperation in the Chinese medicine group and combined treatment group, at 4 weeks postoperation in the model group compared with preoperation (P<0.05). At al time points, serum vascular endothelial growth factor levels were highest in the combined treatment group, but lowest in the model group (P<0.05). Quantitative analysis of vascular proliferation demonstrated that at 8 weeks postoperation, new intimal area, new intimal/medial areas and stenosis rate were highest in the model group, but lowest in the combined treatment group at 8 weeks postoperation (P<0.05). Safety assessment of stem cel transplantation showed morphological structures of the heart, liver, kidneys and pancreas were normal, no necrosis. In a word, the effects of the combined application of bone marrow mesenchymal stem cel s and benefiting-Qi nourishing-Yin and dissolving-congestion prescription were much pronounced in preventing restenosis after percutaneous transluminal angioplasty in dogs with diabetes mel itus rather than single therapy of Chinese medicine. It is a safe and effective treatment to prevent vascular restenosis after percutaneous transluminal angioplasty in dogs with diabetes mel itus.

OBJECTIVETo investigate the clinical value of combination of human epididymis protein 4 (HE4), CA125 and the Risk of Ovarian Malignancy Algorithm (ROMA) in diagnosis of ovarian carcinoma.

METHODSTo detect the serum concentration of HE4 using ELISA and CA125 using ECL in patients of ovarian carcinoma group (n = 119), borderline ovarian tumor group (n = 36), benign ovarian neoplasm group (n = 96) and female healthy control group (n = 53). The ROMA based on the serum level of CA125, HE4 and a woman's menopausal status was used to calculate the predicted probability (PP) and diagnostic results of ovarian cancers.

RESULTSThe receiver operating characteristic (ROC) analysis showed the cut-off value was 67.3 pmol/L (the AUC was 0.906, the sensitivity was 80.7% and specificity was 94.6%). The serum levels of HE4 and CA125 in the ovarian carcinoma group were significantly higher than that in the borderline ovarian tumor group, benign ovarian neoplasm group and female healthy control group (P < 0.01). The serum levels of CA125 and HE4 showed statistically no significant difference between the borderline ovarian tumor group and benign ovarian neoplasm group (P > 0.05). The levels of HE4 and CA125 were reduced significantly in ovarian patients after surgery therapy (P < 0.01). The sensitivity and specificity of HE4 + CA125 combination was 92.7% and 72.5%. The ROMA that can classify patients into high and low risk groups was established as 9.3% in premenopausal and 27.3% in postmenopausal women.

CONCLUSIONSHE4 is a helpful biomarker for ovarian carcinoma diagnosis. Biomarker combination of HE4 and CA125, and applying of the ROMA are helpful to improve the accuracy in diagnosis of ovarian cancers.

Adenocarcinoma, Mucinous ; blood ; diagnosis ; surgery ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Biomarkers, Tumor ; blood ; CA-125 Antigen ; blood ; Cystadenocarcinoma, Serous ; blood ; diagnosis ; surgery ; Cystadenoma, Serous ; blood ; diagnosis ; surgery ; Endometriosis ; blood ; diagnosis ; Female ; Humans ; Menopause ; Middle Aged ; Ovarian Neoplasms ; blood ; diagnosis ; surgery ; Proteins ; metabolism ; ROC Curve ; Sensitivity and Specificity ; Teratoma ; blood ; diagnosis ; surgery ; Young Adult

Objective:To analyze the correlation between serum fibronectin 3(Ficolin-3)levels and early severe bleeding in newly diagnosed acute promyelocytic leukemia(APL)patients.Methods:A total of 125 patients with newly diagnosed APL admitted to Shanxi Bethune Hospital from January 2020 to August 2023 were selected.All patients were given all-trans retinoic acid+arsenic for induction therapy.The severe bleeding events within 30 days of induction therapy(assessed by WHO bleeding score,grade 0,grade 1 and grade 2 were no bleeding or mild bleeding,grade 3 and grade 4 were severe or fatal bleeding)were used as observation endpoints.The serum Ficolin-3 levels was dected by ELISA method,baseline data and other laboratory indicators were counted,and the correlation between serum Ficolin-3 levels and early severe bleeding in newly diagnosed APL patients was analyzed.Results:23 out of 125 APL patients experienced early severe bleeding during induction therapy,including 13 cases of grade 3 bleeding and 10 cases of grade 4 bleeding.There were 102 cases of non-serious bleeding,including 30 cases of grade 0,24 cases of grade 1 bleeding,and 48 cases of grade 2 bleeding.The proportion of serum promyelocytes,white blood cell count,and D-D level in the severe bleeding group were significantly higher than those in the non severe bleeding group(P＜0.05),while the levels of PLT and FIB were significantly lower than those in the non-serious bleeding group(P＜0.05).The serum Ficolin-3 levels in the severe bleeding group were significantly lower than those in the non severe bleeding group before treatment,7 days of treatment,14 days of treatment,and 30 days of treatment(P＜0.05).Confirmed by point two column correlation,serum Ficolin-3 levels were negatively correlated with early severe bleeding in newly diagnosed APL patients before treatment,7 days,14 days,and 30 days after treatment(r values were-0.485,-0.397,-0.304,and-0.183,respectively).The receiver operating characteristic curve(ROC)graph of the subjects was drawn,and the results showed that the area under the curve(AUC)of serum Ficolin-3 levels before treatment and at 7 and 14 days after treatment for predicting early severe bleeding in newly diagnosed APL patients was greater than 0.7,all of which had certain predictive efficacy,and the serum Ficolin-3 level before treatment had the best predictive efficacy.Conclusion:The serum Ficolin-3 levels in newly diagnosed APL patients are associated with early severe bleeding,and the serum Ficolin-3 levels before treatment have a significant advantage in predicting early severe bleeding in newly diagnosed APL patients.

BACKGROUNDHyperbaric oxygen preconditioning (HBO) is a new method of ischemia preconditioning. In this study, we examined its effects on skin flap survival and the mechanisms involved.

METHODSThirty-six rats were divided into three groups: HBO preconditioning, control, and sham groups. An extended epigastric adipocutaneous flap based on the right superficial epigastric artery and vein was raised. A 3-hour period of flap ischemia was induced by clamping the pedicle vessels with a microvascular clamp. At the end of ischemia induction, the clamp was removed and the flap was resutured. Rats in the HBO preconditioning group were treated with HBO four times before surgery. Microcirculation in the skin flap was measured on postoperative days 1, 3 and 5. The size of the flap was measured on postoperative day 5, before the animals were sacrificed. Samples of the skin flap were prepared and stained with hematoxylin and eosin. The levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 in the flap samples were measured.

RESULTSSurviving flap size was significantly higher in the HBO preconditioning group compared with controls, with a reduced inflammatory response and increased perfusion. IL-1, TNF-α, and IL-6 levels in the HBO preconditioning group were lower than in controls.

CONCLUSIONSHBO preconditioning improved flap survival in this ischemia-reperfusion rat model. The mechanisms responsible for this effect may relate to attenuation of the inflammatory response and increased flap perfusion following HBO preconditioning.

Animals ; Graft Survival ; Hyperbaric Oxygenation ; methods ; Ischemia ; surgery ; Male ; Microcirculation ; physiology ; Rats ; Rats, Sprague-Dawley ; Skin ; Surgical Flaps

Adult ; Ankle Injuries ; surgery ; Bone Transplantation ; External Fixators ; Female ; Humans ; Male ; Middle Aged ; Tibial Fractures ; surgery

OBJECTIVETo investigate the differential expression of different types of Smads in keloids, normal scars and normal skins and its possible clinicopathological significance.

METHODSRT-PCR and Western blot methods were used to examine the expression of Smads mRNA and proteins level in 10 cases of keloid, in 10 cases of normal scar and in 10 cases of normal skin tissues and fibroblasts. Fibroblasts of keloid, normal scar and normal skin were cultured in vitro. The expression difference were compared and analyzed by t-test, there was statistical difference when P < 0.05.

RESULTSThe mRNA and protein expression of inhibitory Smad7 were significantly down regulated in keloid compared with normal scar (P < 0.05) and normal skin (P < 0.05). However, no significant difference of the mRNA and protein expression of Smad2, 3 and the protein expression of phosphorylation of Smad2, 3 in keloid, normal scar, normal skin tissues and fibroblasts.

CONCLUSIONSThe decreased expression of Smad7 in keloid might play a significant role in the increased TGF-beta1/Smads signal transduction, which can not be terminated by autologous negative feedback cycle.

Adolescent ; Adult ; Female ; Humans ; Keloid ; metabolism ; Male ; Middle Aged ; RNA, Messenger ; genetics ; Signal Transduction ; Smad Proteins ; genetics ; metabolism ; Transforming Growth Factor beta1 ; metabolism ; Young Adult

OBJECTIVETo study the hypoglycemic effect of the total flavonoids in Leucaena seeds(TF).

METHODExperimental observation was performed by using various diabetic mice induced by alloxan, adrenalin, etc.

RESULTTF could significantly lower the blood glucose levels in Alloxan model mice, adrenalin model mice and hyperglycemic mice, while had no hypoglycemic effect on the normal mice.

CONCLUSIONThe total flavonoids in Leucaena seeds has hypoglycemic effect in diabetic model mice.

Animals ; Blood Glucose ; metabolism ; Diabetes Mellitus, Experimental ; blood ; Epinephrine ; Fabaceae ; chemistry ; Flavonoids ; isolation & purification ; pharmacology ; Hyperglycemia ; blood ; chemically induced ; Hypoglycemic Agents ; isolation & purification ; pharmacology ; Male ; Mice ; Plants, Medicinal ; chemistry ; Seeds ; chemistry

OBJECTIVETo investigate the genotypic diversity of Actinomyces naeslundii in aged people and the relationship between the genotypes of Actinomyces naeslundii and root caries.

METHODSAccording to the inclusion criteria, 20 aged people with root caries and 20 without root caries were chosen into two groups for this study. Two sites were chosen in subjects with root caries: One site was the exposed sound root surface, and the other site was the root caries. In subjects without root caries the sampling site of root surface was exposed. Bacteria were cultured and then identified. Repetitive extragenic palindromic sequence-based polymerase chain reaction (REP-PCR) was used to analyze the genotypic diversity of the Actinomyces naeslundii clinic isolates.

RESULTS299 strains were isolated from the groups, 156 strains were chosen to analyze, belonged to 61 different genotypes. At the site of sound root surface in the subjects with root caries, there were 57 strains with 25 different patterns. At the site of root caries and of sound root surface in subjects without caries, there were 34 strains with 25 different patterns and 65 strains with 26 different patterns respectively. There was the genotypic diversity within Actinomyces naeslundii. There was significant difference in the genotypes in every individual site.

CONCLUSIONMany different genotypes of Actinomyces naeslundii concerned with occurrence of root caries.

Actinomyces ; Aged, 80 and over ; Dental Caries ; Genotype ; Humans ; Polymerase Chain Reaction ; Root Caries ; Tooth Root

Invasion and metastasis are key factors contributing to the high mortality rate of patients with hepatocellular carcinoma (HCC) involving a complex mechanism. In the invasion and metastasis of HCC, miRNAs can serve as either oncogenes or tumor suppressor genes to regulate the differentiation and proliferation of tumor cells being and play important roles in tumorigenesis, angiogenesis, invasion and metastasis. This review summarizes the recent progress in research of the molecular mechanisms by which miRNAs targeting GSK-3β regulate HCC invasion and metastasis and examines the roles of miRNAs in hepatocellular carcinoma cell proliferation, apoptosis, invasion, metastasis, and GSK-3β regulation.