104 cases of children with malignant lymphoma in our hospital from June 1966 to June 1986 are reported. 84 were males and 20 females. Their ages ranged 6 to 12. These patients were 33 HD, 71 NHL. 9 in stage Ⅰ, 29 in stage Ⅱ, 37 in stage Ⅲ and 29 in stage Ⅳ. In this series B group was 55% (57/104). The clinical primary site of intranodular was 75% (78/104) and extranodular 25%(26/104). The treatment methods include rrdiotherapy alone 16, chemotherapy alone 27 and combination 61. The complete remission (CR) was 30% (31/104), partial remission (PR) 58% (60/104). Total repose rate was 88%. The overall 3 and 5 year survival rates were 26.3%(24/91) and 18%(15/83). Survivals of 10 years were 6 cases (no evidence of disease). It is indicated that the primary sites, clinical stages, pathology types, treatment methods and complete remission are the prognostic factors in children with malignant lymphoma. Errly diagnosis, clinical stage and treatment have been discussed briefly. It is emphasized that after treatment, follow-up and maitaining chemotherapy may prolong the life of the patients.

Using proliferating inhibition test, the effects of three cultivars of black mushroom on the growth of gastric carcinoma cell in vitro were observed. We have found that the extracts of all three cultivars of black mushroom had inhibiting effects on the growth of tumor cells in medium at some levels, especially Cr-02 was the most evident one.The results suggest that black mushroom has some anti-tumor effects, and it is worthwhile to study farther for verification.

Objective To assess the feasibility of real-time Bi-Plane in observing normal mitral annular areas in different gestational ages.Methods Routine two-dimensional echocardiography and real-time Bi-Plane were used to observe mitral structure and measure the mitral annular area in 159 fetuses.Pearson correlation analysis was used to evaluate the correlation of the areas with gestational age.Visualization rates and acquisition time were recorded and analyzed statistically.Resuits The mitral annular areas measured by two techniques were both correlated to the gestational age (r=0.71,0.75,both P＜0.05).There were significant differences in visualization rate (x2 =4.375,P=0.036) of mitral annular area and acquisition time (t=6.11,P＜0.05) between routine two-dimensional echocardiography and real-time Bi-Plane.Conclusion Real-time Bi-Plane technique is direct,accurate,convenient and fast in observing mitral prenatally.By using Bi-Plane technique to measure mitral annular area,abnormal morphology changes of mitral valve can be evaluated,so as to provide great application value for clinic.

Female ; Humans ; Incontinentia Pigmenti ; diagnosis ; pathology ; therapy ; Infant, Newborn

OBJECTIVE: To study the preparation process of epirubicin-resveratrol compound liposomes (EPI-RES-L) and investigate its anticancer effects in vitro.

Pulmonary hypertension(PH) is a clinical hemodynamic syndrome characterized by elevation of pulmonary artery pressure and pulmonary vascular resistance,which was presumed mainly due to pulmonary vasoconstriction,remodeling of the pulmonary vessel wall,and thrombosis in situ.Endothelin,nitric oxide,and prostacyclin pathway were three major pathways involved in the pathogenesis.Current therapies interfered with these 3 pathways in combination with conventional measures have prolonged length of life of patients and improved their quality of life significantly.

AIM: To investigate whether Programmed death-1 (PD-1) expression on peripheral CD4~+CD25~(nt/hi)CD127~(lo) regulatory T cells (Treg) was associated with disease progression in HIV-1-infected patients. METHODS: Peripheral blood from 108 HIV-1-infected patients in distinct disease progression statuses and 27 healthy individuals were collected in the present investigation. PBMCs were isolated by centrifugation on Ficoll-Hypaque, followed by staining with anti-CD4-PerCP, anti-CD25-FITC, anti-CD127-PE and anti-PD-1-APC. PD-1 expression on Treg was analyzed by four-color staining flow cytometry. CD4~+T cell absolute counts were determined using Multitest CD3/CD4/CD8/CD45 kit and plasma viral loads were detected on NucliSens EasyQ. All data were analyzed using SPSS14.0 software. RESULTS: In peripheral blood of healthy individuals, Treg expressed PD-1 at very low levels (1.72%±0.65%). In contrast, Treg from HIV-1-infected patients showed a significantly increased PD-1 expression (5.33%±2.24%, P<0.01). Moreover, AIDS patients exhibited statistically higher PD-1 expression on Treg (7.87%±2.23%) than newly HIV-1 infected patients (3.22%±1.01%, P<0.05) and patients in progression to AIDS(5.21%±1.72%, P<0.05). PD-1 up-regulation on Treg was closely correlated with reduced CD4~+T cell absolute counts but elevated plasma viral load. CONCLUSION: Overall, we found that PD-1 expression on peripheral Treg was up-regulated and correlated with disease progression in HIV-1-infected patients for the first time. These findings not only extend our understanding of how Treg functions in HIV-1-infected patients but also support the notion that blocking PD-1/PD-L1 interactions may represent a potential therapeutic strategy for HIV-1-infected patients.

In constructing the system of medical functional teaching and enhancing the quality and ability of students,we adjust the content and method of teaching in practice,and try to find students'problems in study through questionnaire survey and promote teaching reform and disciplines construction.

The process of tumorigenesis,local invasion and distant metastasis will produce a series of molecular changes,these genetic mutation or abnormal expression of molecules play a promoting role in hypercoagulation.At the same time,hypercoagulation also increases the risk of tumor progression.It is important to understand their specific processes and the molecular role,and it can provide theoretical basis for better management of patients,and has significance for developing more effective and security new anti-clotting drugs.