Autism in children is a complex disease of developmental disorder in nervous system,characterized by social deficits,impaired language and communication,stereotyped and repetitive behaviors.Studies indicate oxidative stress is associated with autism.Because of genic and environmental factors,children with autism are susceptible to oxidative stress.Oxidative stress induce the development of autism through abnormal metabolism of the membrane lipid,inflammation and excitotoxicity,autoimmunity,energy metabolism disorder,et al.Anti-oxidation might be a safe and effective therapy for autism.

Objective To compare and analyze two methods for determination of vaccine particle content in freeze-dried human rabies vaccine(Vero cells)bulk and final bulk,and to provide experimental basis for establishing standard detection method for particle content and purity of rabies vaccine products.Methods The samples were subjected to vaccine particle cracking,BCA quantification,and size exclusion chromatograghy-high performance liquid chromatography(SEC-HPLC)analysis. BCA-SEC relative area method and BCA-SEC vaccine particle standard curve method were used to detect the total protein concentration of the bulk and final bulk,separately,and the content of vaccine particles was quantitatively analy-zed. Finally,the detection results of the two methods were compared and analyzed.Results The content of vaccine particles in freeze-dried human rabies vaccine bulk was determined to be within 256-305 μg/mL by the two methods,with an average value of 267-285 μg/mL,and the relative standard deviations(RSDs)ranged from 5. 8% to 10. 2%,with good consistency between two methods. The content of vaccine particles in freeze-dried human rabies vaccine final bulk was determined to be in the range of 149-169 μg/mL by the two methods,with an average value of 152-164 μg/mL,and the two methods showed the RSDs between 0. 9%-4. 7% with good consistency.Conclusion The measured value by BCA-SEC standard curve method deviates less from the expected value and is closer to the actual situation of samples,so it is recommended to use this method as a reference for enterprises.

Objective: To explore the effects of Layhey’s and Lawrence’s reconstruction procedures on nutritional status and digestive symptoms following total gastrectomy for gastric cancer.Methods: To analyze retrospectively 146 patients performed total gastrectomy for gastric cancer.Among them,83 cases were operated by Lahey’s reconstruction procedures and 63 cases by Lawrence’s.It was focused on the comparision of the body weight changing,nutritional status and digestive symptoms in 6 months after operation.Results: In 6 months after operation,the weight loss of Lawrence group was less than that of Lahey group significanly.For the level of total protein and albumin at that time,they increased in Lawrence group,but decreased in Lahey’ group,and the difference was significant.The changing tedency for hemoglobin level in Lawrence group was less than that of Lahey group,but there was no significant difference between them.For the quantity and frequency of food intake,there was no significnat difference between them.In Lawrence group,the number of patients with vomiting were less than that in Lahey’group significantly.Conclusion:The Lawrence reconstruction procedure is more reasonable following total gastrectomy for gastric cancer.

Objective To observe and analyze the clinical effect of Modified Decoction for the treatment of lung -qi and spleen -qi deficiency syndrome in patients with stable stage of chronic obstructive pulmonary disease (COPD).Methods 86 patients with lung -qi and spleen -qi deficiency syndrome in stable stage of COPD were divided into the observation group (n =43) and control group (n =43) according to a random number table method. The control group was treated with conventional western medicine therapy,the observation group was given modified Bufei decoction treatment on the basis of western medicine treatment.The clinical therapeutic effect of two groups was assessed.Results The effective rate of the observation group was 83.72%,which was significantly higher than 72.09% of the control group,the difference was statistically significant (χ2 =3.9289,P <0.05).After treatment,the FEV1 ,FEV1 /FVC,FEV1% expected values of the two groups were significantly higher than those before treatment (t =3.6984,3.6915,3.7431,4.2675,4.6821,5.0567,all P <0.05).After treatment,the FEV1 ,FEV1 /FVC, FEV1% predicted value of the treatment group were (2.45 ±0.35)L/s,(63.91 ±7.42)%,(47.29 ±7.52),which were significantly higher than those of the control group (t =3.6884,3.7581,4.1957,all P <0.05).After treatment, the levels of IL -8 and TNF -αwere not significantly changed in the control group.The levels of IL -8 and TNF -αin the observation group were significantly lower than those in the control group (t =8.864,9.571,all P <0.05). Conclusion In COPD patients of stable stage with lung -qi and spleen -qi deficiency syndrome,plus or minus fill lung soup can effectively relieve clinical symptoms and improve pulmonary function of patients,reduce the acute attack,and improve the quality of life of patients,it is worthy of clinical application.

Objective: Our aim was to evaluate the biocompability of porcelain compounded with nanostructured material. Methods：The proliferation of the NIH3T3 cells were investigated using a tetrazolium-based colorimetric assay(MTT assay).Maceration extract of four tested materials were added into culture plate .The samples were tested with ultraviolet spectrophotometer in 490 wavelength at 1，3，5，7 days after the addition of extract. Morphology of these cells was also observed with phase-contrast microscope. Results： There was no obvious difference among three kinds of prosthetic materials in the optical absorbance and the cells growing in the extract showed normal morphology. Conclusion: The toxicity graduation of the porcelain compounded with nanostructured material is 0. This material has a good biocompability and can be used clinically.

The "opening-closing-pivoting" theory on the six meridians is one of the difficult points in the study of Shang Han Lun (Treatise on Cold Diseases). The authors give an explanation on the space and time differentiation of three-yin and three-yang as well as the "opening-closing-pivoting" theory based on the predecessor's experience, and then analyze the physiological and pathological characteristics of the six-meridian disorders.

Objective To investigate the clinical efficacy and safety of topical corticosteroids as adjunctive plan in the therapy of bacterial corneal ulcers (BCU).Methods 62 eligible patients with confirmed BCU were screened and divided into 2 groups randomly.Both groups were treated with topical tobramycin for 3 weeks,the observation group received topical tobramycin for at least 48 hours and then treated with 0.02％ fluorometholone for 3 weeks,and decrement setp by step.Best spectacle corrected visual acuity(BSCVA),fluctuation of intraocular pressure (IOP) before and after therapy,time to corneal reepithelialization and clarity,severe adverse event were observed.Results After 3 weeks,BSCVA of both groups increased compared to their baselines,however,no significant difference between the two groups.Patients whose IOP exceed 21mm Hg in the control group was statistically more than that in the observation group(x2 =7.272,P ＜ 0.05).Follow up for 3 months,increasing of BSCVA in the observationgroup was significantly higher than the control group(t =2.388,P ＜ 0.05) ;and lOP of both two groups almost recuperated to baselines.Although the time to corneal reepithelialization of the control group (11.3 ± 4.5) d was shorter than observation group [(14.7 ± 5.2) d] (t =2.707,P ＜ 0.05),the recovery of corneal clarity in observation group was superior to control group (x2 =8.207,P ＜ 0.05).In addition,no severe adverse events were observed during this period.Conclusion Although prolong the healing time of corneal epithelium,corticosteroids as an adjunctive plan in the therapy of BCU may reduce immune-mediated damage,decrease scarring,control IOP fluctuation and improve BSCVA,but with no safety concerns.

Objective To explore diagnostic value of the immune intensity of platelet ?-amyloid precursor protein(APP) and isoforms proportion for Alzheimer's disease(AD).Methods The APP immune intensity of platelets was evaluated by flow cytometric detection and the isoforms proportion of APP130/APP106 was also detected by Western Blot analysis in 31 AD cases, 22 vascular dementia (VD) patients , 28 neural degeneration patients and 30 healthy elders.Results There was no significant difference of APP immunity in the each group. The ratio of APP130/APP106 was significantly lower in the AD group compared with the groups VD, degenerative disorder and healthy elders(all P

Objective:To explore the mediating effect of attitudes going into the of undergraduates world or leaving the mundane world between internet addiction disorder (IAD) and undergraduates' well-being. Methods:603 undergraduate students were surveyed with the Scale of Going into the World and Leaving the World, the Scale for Internet Addiction and the Multiple Happiness Questionnaire. Correlation, regressions and structural equation were adopted. Results:The rate of IAD in our sample was 9.1%, which was higher in male (47persons, 13.2%) than in female (8persons, 3.2%)(?2=16.47,P

Objective To investigate the distribution of intramuscular nerves in the different skeletal muscles of the rabbit. Methods Muscle architecture and modified sihler's neural staining methods were used. Results The nerve branches of innervating flat M.pectoralis major arose from the anterior pectoral and posterior pectoral nerve.The former innervated cross fibers mainly,it penetrated the middle of muscle belly and formed a “U-Shaped” nerve ansa.The latter innervated oblique fibers mainly.There were many anastomoses between them.The nerve of pennate M.plantaris derived from tibial nerve.After entering muscle,the nerve trunk gradually divided into many primary branches toward medial and lateral fibers,these branches then subdivided into numerous arboroid second and terminal branches toward all of muscle fibres;The nerve of innervating spindle M.extensor digitorum longus came from N.fibularis communis.Two extramuscular nerve trunk were seen.Superior trunk mainly innervated those muscle fibers of inserting in the second toe,Inferior one mainly distributed to the fibers of rest within this muscle.Conclusion The spatial arrangement of the muscle fibres were related to distribution of the intramuscular nerves;Walking of intramuscular nerve branches had two patterns which run perpendicular and/or parallel to muscle fasciculi.