BACKGROUND:Several studies have demonstrated that JAK2 kinase plays an important role in myocardial protection by ischemic preconditioning and medicine preconditioning. OBJECTIVE:To investigate the effects of exercise preconditioning on JAK2 mRNA and protein expression in the rat heart and the underlying mechanism of action. METHODS:SD rats were randomly divided into control, exhaustive, exercise preconditioning, and exercise preconditioning + AG490 groups. Rats in the control and exhaustive groups were fed routinely for 3 days. Rats in the exercise preconditioning and exercise preconditioning + AG490 groups were subjected to intermittent treadmil exercise for 3 successive days to establish exercise preconditioning animal models. Rats in the exercise preconditioning + AG490 group were intraperitonealy injected with JAK2 inhibitor AG490 at 10 minutes before exercise preconditioning. After 3 days, rats in the exhaustive, exercise preconditioning, and exercise preconditioning + AG490 groups were subjected to exhaustive treadmil exercise. Serum levels of lactate dehydrogenase and creatine kinase isoenzyme were determined. JAK2 mRNA and protein expression in the rat myocardial tissue was detected by real-time fluorescent quantitative PCR and immunohistochemistry.RESULTS AND CONCLUSION:Compared with the control group, serum levels of actate dehydrogenase and creatine kinase isoenzyme were increased, and JAK2 mRNA and protein expression in the myocardial tissue was significantly increased in the exhaustive group (P < 0.05). Compared with the exhaustive group, serum levels of actate dehydrogenase and creatine kinase isoenzyme were decreased (P < 0.05), while JAK2 mRNA and protein expression in the myocardial tissue was increased in the exercise preconditioning group (P < 0.05). Compared with the exercise preconditioning group, serum levels of actate dehydrogenase and creatine kinase isoenzyme were increased (P < 0.05), while JAK2 mRNA and protein expression in the myocardial tissue was decreased in the exercise preconditioning + AG490 group (P < 0.05). The results confirm that exercise preconditioning plays its cardioprotective role through activating JAK/STAT signaling pathway, up-regulating the expression of JAK2 mRNA and protein in the cardiac tissue and aleviating myocardial ischemia injury.

The case of LI Li-yun involves the right of life,the right of informed consent and the special right to intervene from the inspection of right.By analyzing the concepts of three kinds of rights and discussing the conflicts between the right of informed consent,the right of life,and the special right to intervene,it presents the principles of equity of right and the measures of legal insurance which are essential conditions to end this kind of tragedy.

0.05).CONCLUSION:Ketoconazole may play its role in treating OHSS by reducing the expression of VEGF.

Objective To assess the feasibility of real-time Bi-Plane in observing normal mitral annular areas in different gestational ages.Methods Routine two-dimensional echocardiography and real-time Bi-Plane were used to observe mitral structure and measure the mitral annular area in 159 fetuses.Pearson correlation analysis was used to evaluate the correlation of the areas with gestational age.Visualization rates and acquisition time were recorded and analyzed statistically.Resuits The mitral annular areas measured by two techniques were both correlated to the gestational age (r=0.71,0.75,both P＜0.05).There were significant differences in visualization rate (x2 =4.375,P=0.036) of mitral annular area and acquisition time (t=6.11,P＜0.05) between routine two-dimensional echocardiography and real-time Bi-Plane.Conclusion Real-time Bi-Plane technique is direct,accurate,convenient and fast in observing mitral prenatally.By using Bi-Plane technique to measure mitral annular area,abnormal morphology changes of mitral valve can be evaluated,so as to provide great application value for clinic.

BACKGROUND: Acinetobacter baumannii (AB) bacteremia is an increasingly common and often fatal nosocomial infection. Identification of morbidity and mortality risk factors for AB bacteremia in emergency department (ED) patients may provide ways to improve the clinical outcomes of these patients. METHODS: The records for 51 patients with AB bacteremia and 51 patients without AB infection were collected and matched in a retrospective case-control study between 2013 and 2015 in a singlecenter ED. Risk factors were analyzed by Chi-square and multivariate logistic regression statistical models. RESULTS: A signifi cant risk factor for morbidity was the presence of a central venous catheter (CVC) (P<0.001). The mortality rate for the 51 patients with AB bacteremia was 68.6%. Risk factors for mortality were the presence of a CVC (P=0.021) and an ED stay longer than two weeks (P=0.015). CONCLUSION: AB infections lead to high morbidity and mortality. The presence of a CVC was associated with higher morbidity and mortality in patients with AB bacteremia. Avoiding CVC insertions may improve outcomes in ED patients with AB bacteremia.

Adult ; Chromosome Aberrations ; Female ; Humans ; Infant, Newborn ; Karyotyping ; Male ; Pedigree

Adult ; Drugs, Chinese Herbal ; therapeutic use ; Health Behavior ; Humans ; Infertility, Male ; therapy ; Integrative Medicine ; methods ; Life Style ; Male ; Phytotherapy ; Sperm Motility ; drug effects

Adult ; Genetic Predisposition to Disease ; Humans ; Male ; Motion Sickness ; genetics ; Naval Medicine ; Peptidyl-Dipeptidase A ; genetics ; Polymorphism, Genetic ; Young Adult

OBJECTIVETo observe the efficacy of encephalopathy therapeutic tongue acupoint apparatus (ETTAA) in the treatment of autism.

METHODSEighty-four children of autism were randomly divided into a tongue acupuncture group (group A) and a conventional training group (group B), 42 cases in each group. The behavior training and sensory integration training were carried out in group B and the ETTAA was added in group A. The apparatus was switched on for 20 min every time and 3 times a day. Treatment of two months were carried out in both groups. The score of childhood autism rating scale (CARS) and clinical efficacy in both groups were observed before and after treatment.

RESULTSAfter treatment, the CARS in both groups were significantly reduced (42.39 +/- 6.86 vs 32.15 +/- 5.12, P < 0.001; 44.58 +/- 6.76 vs 39.72 +/- 7.11, P < 0.05), which was more significant in group A (P < 0.01). The totally effective rate in group A [90.5% (38/42)] was superior to that in group B [66.7% (28/42), P < 0.01].

CONCLUSIONThe clinical efficacy in tongue acupuncture group is apparently superior to that in conventional training group, ETTAA combined with conventional training have a better curative effect in the treatment of autism.

Acupressure ; Acupuncture Points ; Autistic Disorder ; therapy ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Tongue ; anatomy & histology