Objective To detect the expression of YB-1 and to research the relationship in the occurrence and development in the cervical squamous carcinoma(SCC) tissues.Methods Immunohistochemistry(IHC) envision was used to detect the expression of chronic cervicitis,CIN Ⅰ,CIN Ⅱ-Ⅲ and SCC.The relationship of YB-1 in the clinical pathological parameters of SCC were analyzed.Results YB-1 was mainly located in the nucleus in squamous cell,sometimes in the cytoplasm.The YB-1 protein did not expression in chronic cervicitis.In CIN Ⅰ,CIN Ⅱ-lⅢ and SCC,the positive expression had a gradual increasing trend.The expression of YB-1 was satistically significant in four groups (P＜0.05).The chronic cervicitis group,CIN Ⅰ groupCIN Ⅱ-Ⅲ group compared with the SCC group restivelly,the difference was statistically significant (P＜0.05).From spearman rank correlation analysis:the expression of YB-1 was positively correlated with the degree of cervical lesions (P＜ 0.05).In the cervical squamous carcinoma group,the expression of YB-1 was not associated with clinical pathological index of SCC patients(P＞0.05).Conclusion The change of the quantity of YB-1 protein is closely related to cervical squamous cell carcinoma.

Equisetin (EQST) belongs to polyketide (PKS)-nonribosomal peptide synthetase (NRPS) type compound with an inhibitory effect of 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) enzyme activity. This study investigated anti-obesity effect and insulin resistance improvement effect of EQST on high-fat diet (HFD)-induced ob/ob mice model. EQST treatment effectively reduced the body weight gain, fat weight gain and blood lipid content of model mice. All animal experiments were approved by the Medical Ethics Committee of Capital Institute of Pediatrics. EQST alleviated adipose tissue expansion and hepatic ballooning degeneration of model mice, and also effectively controlled the blood glucose content after glucose load and insulin load, showed a significant improvement in obesity and insulin resistance. EQST inhibited adipogenic proteins fatty acid-binding protein 4 (FABP4) and peroxisome proliferators-activated receptor γ (PPARγ), and upregulated thermogenic protein (uncoupling protein 1, UCP1) through suppressing 11β-HSD1 protein expression. In addition, EQST widely upregulates mitochondrial respiratory metabolism related proteins in adipose tissue and may improve insulin resistance through phosphatidylinositol-3-kinase (PI3K) pathway. Therefore, EQST plays an anti-obesity role by promoting adipose tissue thermogenesis and improving insulin resistance, which may provide reliable clues for improving obesity and diabetes.

Adenoid hypertrophy is a disease that mostly occurs among children of 3-5 years old. It is caused by repeated inflammation and infection of nasopharynx and its adjoin parts, or the adenoid itself, which will finally leads to pathological hyperplasia of adenoid. With so much information we have acquired about this disease, its specific mechanism remains unknown. In recent years, some researches have indicated that adenoid hypertrophy may have something to do with extra-gastroesophageal reflux, in which pepsin plays a very important role, and pepsin will do a series of pathological damages to the upper airway as it reaches the upper respiratory tract. Based on relative domestic and foreign literature, this paper attempts to make a review about the relationship between gastroesophageal reflux and adenoid hypertrophy.
Adenoids ; pathology ; Child ; Gastroesophageal Reflux ; complications ; Humans ; Hypertrophy ; complications ; Nasopharynx ; pathology ; Pepsin A ; metabolism

Because the biology characteristic of HIV is extremely different from that of other microorganisms, HIV vaccine research has being faced with unprecedented difficulties and challenges. In the last 20 years, HIV vaccine research has been carried out mainly with two strategies, i.e., neutralizing antibody based and cellular immunization based; however, substantive breakthroughs haven't been achieved until now. Inducing effective neutralizing antibody is always an important strategy in traditional vaccine research, but this strategy is much less efficient in HIV vaccine research because of high variation and much subtypes of HIV. In recent years, the discovery of broadly neutralizing monoclonal antibodies and elucidation of their corresponding antigen epitopes have brought new hopes for the development of neutralizing antibody based HIV vaccine. Reviewing these advancements will be helpful for reconsidering HIV vaccine development with a better strategy.

China ; Humans ; Industry ; statistics & numerical data ; Lead ; blood ; Occupational Exposure

Objective To summarize the tending physique of hysteromyoma patients and thus to provide the clinical prevention and treatment methods for the disease.Methods Clinical epidemiology investigation and study methods were adopted to design the unified investigation and study table.Questionnaire survey were performed stochastically to 150 hysteromyoma patients and 150 normal females.Various physique characteristics were recorded.Results Ecchymosis physique were commonly seen in hysteromyoma patients,accounting for 36.7％ in all patients.Conclusion The occurrence of hysteromyoma was closely related to ecchymosis physique.

Objective To construct the eukaryotic expression vector of linker for activation of T cells(LAT) gene in mouse. MethodsLAT cDNA of mouse mononuclear cells was amplified with polymerase chain reaction.The fragment was inserted into the eukaryotic expression vector of pCMV-HA plasmid.The recombinant plasmid was verified by DNA sequencing and used to transfect lymphocytes of the asthmatic model. Results The length of amplified fragment was 729 bp.The sequence of LAT gene was confirmed by Genbank.The LAT protein could be detected in the lymphocytes of asthmatic model.Conclusion The recombinant eukaryotic expression vector pCMV-HA-LAT can be successfully constructed.

Objective To evaluate the efficiency and safety of domestic rapamycin-eluting stent(Firebire stent) in the treatment of coronary heart disease.Methods Coronary angiography and stenting were carried out through femoral artery approach in 331 patients with coronary heart disease.The incidences of in-hospital major adverse cardiac events were recorded all the patients were followed up for 1 year.The follow-up angiography was carried out at at 6-9 months after discharge.Results A total of 597 domestic rapamycine-eluting stents were implanted in 331 patients.One patient died of pump failure after primary PCI and another patient required emergency CABG because of coronary artery rupture.NSTEMI happened in 3 patients after PCI.The incidence of in-hospital MACE was 1.5%.Three hundred and one patients finished a mean follow-up of 12.3?3.2 months.During the follow up period,non-fatal myocardial infrarction was recorded in 1 case,recurrent angina in 16 cases and target lesion revascularization in 5 cases.The overall MACE rate was 4.3%.Follow-up angiography was completed in 110 patients and the restenosis rate was 5.5%(6/110).Conclusion Domestic rapamycin-eluting stent(Firebird stent) was safe and effective in the treatment of coronary heart disease and may provide a higher cost-effectiveness due to its relatively low price.However,its long-term effect needs more studies to verify.

Objective To study the adaptability of rat' skeletal muscle to medium load on endurance training. Methods 40 male Wistar rats were used in an upward-slope running with medium load. Then the ultrastructural changes of the soleus muscle and IGF-1 expression in different training stages were observed. Results The pathologic change of muscular tissues suggested that there was kinesic injury in rat soleus muscle, and the injury happened most seriously in the third week, when muscle structure experienced a relative 'fragile' period. At the same time, there was an obvious increase of IGF-1 in muscular tissues, which indicated that the muscle started to repair of itself immediately after injury. The abnormity rate of Z-cure and IGF-1 level began to decline gradually after 4 weeks, meanwhile the pathologic change reduced. Conclusion After been injured, the pathologic changes of muscle underwent a course of going serious then reducing accompanied with the reparation. The fact indicated that rat skeletal muscle showed a good adaptability to the injury accumulation.

Objective To compare the effectiveness of running training, swimming training and intensified cyclic training by means of morphometry and to determine the most effective training mode to develop the aerobic ability of the skeletal muscle. study the adaptability of rat skeletal muscle to different endurance training modes. Methods Twenty-five healthy male Wistar rats of 8 weeks old were divided into five groups, 10 of them having no exercise be divided into control group 1 and control group 2, others took part in running exercise, swimming exercise and intensified cyclic exercise respectively. The rats in control group1 were killed at the beginning of the exercise. The free activity group was taken as the control group 2, the rats were not given any interventions, they could move freely in the cage and be killed at the end of the exercise (n=5). The rats in running group were forced to take part in an upward-slope (+5?) running task of medium load, the rats should kept running at the speed of 16m per minute for 45 minutes. The rats in swimming group were forced to keep swimming in the pool for 45 minutes, The rats in intensified cyclic group were kept running for 30 minutes (the running mode was same to the running group), then had a rest for 15 minutes, and at last the rats were forced to keep swimming in the pool for 30 minutes. The rats in training groups were trained for 6 days every week with 1-day rest for 8 continuous weeks. We observed the change of aerobic ability of the soles muscle in different training modes. Results The three kinds of endurance training developed the aerobic metabolism ability of skeletal muscle in rats effectively. The intensified cyclic training can do better than swimming training and running training in promoting remodeling reconstruction of skeletal muscle, and developing the muscle, function. Conclusion The intensified cyclic training can shorten the period of remodeling reconstruction of skeletal muscle, and more effective in developing the aerobic ability of the skeletal muscle than other endurance training modes.