Objective To investigate the changes of immunologic functions and life qualities after the auto-cytokine induced killer (CIK) cells combined with interleukin-2 (IL-2) being transfused back to the patients with non-small cell lung cancer (NSCLC).Methods Thirty-eight NSCLC patients were enrolled,and 19 patients received not only chemotherapy and radiotherapy but also adoptive immunotherapy with CIK cells combined with IL-2 compared with the other 19 patients who only accepted radiotherapy and chemotherapy.The changes of immunologic functions and life qualities after the CIK cells combined with IL-2 being transfused back to the patients with NSCLC were observed.Immune targets of the treatment group and control group,such as peripheral blood CD3 +,CD4 +,CD8 + T cell percentage,ratio of CD4 +/CD8 + and Th1/Th2,were observed at the end and after 3 month of the radiation and chemotherapy.Results Both of the two groups were neither having obvious adverse reactions.After CIK cells combined with IL-2 treatment,the life qualities of patients improved,such as 18 cases of mental improvement,15 cases of appetite improvement,12 cases of sleep improvement,and 3 cases of fatigue symptoms improvement.The percentage of CD3 +,CD4 + cells in the treatment group increased [(66.39 ± 9.22) ％ vs (42.98 ± 7.23) ％,t =5.45,P =0.00 ; (32.27 ± 3.75) ％ vs (26.38 ±2.51)％,t =5.73,P =0.00],the percentage of CD8 + cells declined [(17.51 ± 1.85)％ vs (20.90 ± 2.31) ％,t =5.21,P =0.00],the rate of CD4 +/CD8 + increased and the rate of Th1/Th2 obversed [(1.86±0.32) vs (1.27±0.19),t=7.13,P=0.00;(1.15±0.48) vs (0.91 ±0.30),t=2.42,P=0.02].There were significant differences between the two groups.Conclusion Adoptive immunotherapy with CIK cells combined with IL-2 is safe in clinical,which can improve the immunologic functions and life qualities of the patients with NSCLC.

Potential pathogenesis is defined a pathology linking without corresponding symptoms, but as a part of pathogenesis which has important affects on the formation and development of main syndrome. The proposal of potential pathogenesis is a supplement of traditional pathogenesis theory, and has practical signifi cance to the improvement of syndrome pathogenesis theory and raise of the level of determination of treatment based on differentiation of symptoms and sign. Potential pathogenesis has characteristic of logicality, reality and provability. We can understand the relationship between the evaluation of modern medical laboratory and disease sydrome, the principle of batch formula and the skill of medicines compatibility with the potential pathogenesis theory. The explication of potential pathogenesis theory will promote the in-depth exploration of disease syndrome and pathogenesis.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Tennis Elbow ; therapy

Objective: To investigate the effects of bovine plasma fibronectin on the proliferation and differentiation of rat osteoblasts. Methods:Rat osteoblasts were cultured and identified. AlamarBlue were used to determine the effect of various concentration of bovine plasma fibronectin on the proliferation of the osteoblasts. ELISA method was used to determine the alkaline phosphatase activity of the cells. Results: Fibronectin stimulated the proliferation of rat osteoblasts at 40 ?g/ml (P

Endosonography ; Esophageal Neoplasms ; diagnostic imaging ; radiotherapy ; Humans ; Image Processing, Computer-Assisted ; Lymph Nodes ; diagnostic imaging ; pathology ; Positron-Emission Tomography ; Radiotherapy Dosage ; Radiotherapy Planning, Computer-Assisted ; methods ; Radiotherapy, Conformal ; Tomography, X-Ray Computed ; Tumor Burden

? AIM: To investigate the efficacy and safety of phacoemulsification combined with intraocular lens implantation in the treatment of shallow anterior chamber with cataract.?METHODS: Retrospective case series. From February 2014 to July 2015 in our hospital,65 eyes in 65 patients with cataract were enrolled and divided into mild and high risk of shallow anterior chamber group. Best-corrected visual acuity ( BCVA ) , intraocular pressure ( IOP ) , central anterior chamber dept ( CACD ) , angle opening distance ( AOD ) , complications pre- and post treatment, were observed and analyzed as outcome measures.?RESULTS: In this study, the mild shallow anterior chamber group included 34 eyes; postoperative BCVA were improved in 29 eyes, with 4 eyes remaining stable and decreased in 1 eye; BCVA was improved in 16 eyes, with 10 eyes remaining stable and decreased in 5 eyes in high risk of shallow anterior chamber group postoperatively. BCVA had a better prognosis in the mild shallow anterior chamber group than another group ( t=-2. 956, P<0. 05). Meanwhile, IOP decreased by 5. 71± 2. 07mmHg and CACD increased by 1. 37 ± 0. 38mm in the mild shallow anterior chamber group, by 9. 77±4. 04mmHg and 1. 67±0. 43mm respectively in high risk group, and the difference has statistical significance ( t=-5. 02,-3. 04; P<0. 05). The mean preoperative nasal AOD500 was 200. 57± 33. 74μm, and they were 346. 62 ± 101. 37μm and 410. 75 ± 137. 48μm and 398. 69 ± 122. 28μm respectively at postoperative 1d, 1 and 3mo, and all nAOD500 comparing with preoperative were increased obviously, and the difference has statistical significance (F=203. 75, P<0. 01). And AOD500 at temporal, superior and inferior presented similar trends. Complications were corneal edema ( 5 eyes ) , transient intraocular hypertension ( 2 eyes ) , posterior capsular opacification ( 4 eyes ) , and posterior capsular rupture (1 eye).?CONCLUSION:Micro incision cataract surgery is useful, effective and safe in patients with cataract and shallow anterior chamber which can stabilize or improve BCVA, reduce IOP, deepen CACD and open the anterior chamber angle.

Objective: To evaluate the clinical efficacy of SKy bone expander system in percutaneous kyphoplasty for treatment of osteoporotic verterbral compression fracture. Methods: Twenty-two patients (aged 62-90 years, 32 vertebrae) underwent percutaneous kyphoplasty using SKy bone expander system. The bone cement was injected into the collapsed vertebrae. The vasual analogue scale (VAS) and complications were recorded during follow up. Results: The operations were successful in all patients via unilateral or bilateral approach. The operation time ranged from 30 to 120 min. The mean volume of cement injected into each vertebra body was (4.8±1.1) ml, ranged from 3.1 to 6.8 ml. Extravertebral leakage of bone cement was observed in two vertebrae with no symptoms. All patients had their pain relieved; the VAS was 7.6±0.8 before operation,3.5± 0.5 one day after operation, 2.8±0.6 one week after operation, and 2.4±0.6 one month after operation, with significant difference found between preoperation and postoperation (P<0.05). The symptoms of the patients were improved during a follow up of 12-26 months without recurrence. Conclusion: Application of SKy bone expander system in percutomeous kyphoplasty can relieve pain and restore the vertebrae height; it may be an effective treatment for aged patients with osteoporotic verterbral compression fracture.

Vascular calcification (VC) is a chronic systemic vascular disease characterized by abnormal deposition of hydroxyapatite minerals in the vascular system and is closely associated with aging, diabetes, atherosclerosis, and chronic kidney disease. Perivascular adipose tissue (PVAT), a special type of adipose tissue that surrounds blood vessels, is thought to be a supportive component of the vascular structure and is capable of playing a role in homeostatic regulation during vasodilatation and contraction. Currently, there is growing evidence that perivascular adipose tissue acts as an endocrine and paracrine organ and interacts closely with cellular components of the vascular wall, which may be involved in the development of vascular calcification. This article reviews the role of perivascular adipose tissue in the pathophysiological process of vascular calcification and its potential as a target for therapeutic intervention, with the aim of providing new ideas for the prevention and treatment of vascular calcification.

Animals ; Cell Culture Techniques ; methods ; Cells, Cultured ; Cryopreservation ; Male ; Mice ; Spermatogonia ; cytology ; Stem Cells ; cytology

OBJECTIVETo observe the effect of Oxymatrine on left cardiac function and ventricular remodeling in rabbits after acute myocardial infarction.

METHODSLigation of the left anterior descending artery was adopted to establish acute myocardial infarction model, forty eight rabbits were randomized into the sham operation group, model group and Oxymatrine group. Eight models were successfully established in each group. the sham operation group and model group were given conventional feed. Oxymatrine were gavage administration 0.5 ml/100 g, once a day, lasted for 4 weeks. Respectively in postoperative week, and three weeks, to observe the Oxymatrine on cardiac output (CO), left ventricular end systolic pressure (LVESP), left ventricular end-diastolic pressure (LVEDP), left indoor pressure change rate peak (dp/dtmax)), and left ventricular cavity internal diameter (D), ventricular weight index (VWI), ventricular weight (VW).

RESULTSLeft ventricular anterior wall was from red to deep purple, electrocardiogram showed II guide ST-segment camber up ≥ 0.25 mv. Postoperative week in Oxymatrine group compared with model group, LVESP increased significantly (P < 0.01), LVEDP decreased obviously (P < 0.01); After three weeks in Oxymatrine group compared with model group, VW, VWI decreased (P < 0.05), D significantly reduced (P < 0.01); LVESP increased significantly (P < 0.01), LVEDP decreased obviously (P <0.01); dp/dt(max), CO increased (P < 0.05).

CONCLUSIONAfter acute myocardial infarction in rabbit Oxymatrine can improve the left ventricular reconstruction parameters, increase cardiac output, and improve cardiac function.

Alkaloids ; pharmacology ; Animals ; Cardiac Output ; Heart ; drug effects ; Myocardial Infarction ; pathology ; Quinolizines ; pharmacology ; Rabbits ; Ventricular Remodeling ; drug effects