1.Culture of cryopreserved mouse spermatogonial stem cells.
Lian-jun LI ; De-xue LI ; Xue-ming ZHANG
Chinese Journal of Applied Physiology 2004;20(2):145-208
Animals
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Cell Culture Techniques
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methods
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Cells, Cultured
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Cryopreservation
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Male
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Mice
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Spermatogonia
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cytology
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Stem Cells
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cytology
4. Research progress of nlrp3 inflammasome in diabetic cardiomyopathy
Chinese Pharmacological Bulletin 2019;35(8):1051-1054
Diabetic cardiomyopathy( DCM) is one of the important cardiovascular complications of diabetes mellitus. Metabolic disorders, mitochondrial damage, cardiomyocyte apoptosis and autophagy are involved in the development of DCM. Recent research demonstrates that NLRP3 inflammasome plays a vital role in the pathogenesis of DCM, and has emerged as a potential tar get for the treatment of DCM. In this article, research progress in NLRP3 inflammasome and DCM has been reviewed, aiming to provide novel therapeutic idea for the treatment of DCM.
5. Activity regulation mechanisms of protein phosphatase 2A and its role in Alzheimer’s disease
Journal of International Pharmaceutical Research 2016;43(1):39-43
Protein phosphatase 2APP2Ais the main serine/threonine protein phosphatase of the brain, and it is involved in many physiological and pathological processes in the cell. Particularly in the pathogenesis of Alzheimer’s diseaseAD, PP2A is closely related to the main pathological features of AD. Deregulation of PP2A enzymes correlates with increased tau phosphorylation, the formation of amyloid precursor protein, and the missing of neurons. PP2A as the target of drug development may bring new hope for the treatment of AD. This review introduces the structure and activity regulation of PP2A, and the role of PP2A in AD.
6.The relationship among the matrix metalloproteinase-3,carotid artery plaque and ischemic cerebrovascular disease
Dongfang LI ; Junlin JI ; Guanglai LI ; Guofang XUE ; Xuejun XIE ; Yuheng PEI ; Xia LIAN
Journal of Chinese Physician 2011;13(3):302-304
Objective To investigate the relationship between the matrix metalloproteinase-3(MMP-3)and the stability of carotid artery plaque,and explore MMP-3's prediction role on the attack and relapse of acute ischemic cerebrovascular events.Methods 100 patients with the first ever acute cerebral infarction,100 patients with chronic cerebral circulation insufficiency(CCCI)and 40 persons without cerebrovascular diseases were enrolled in this study.According to the carotid ultrasound examination,100 cerebral infarction patients were divided into three subgroup: unstable plaque group(45 patients,mixed plaque,soft plaque),stable plaque group(35 patients,plaque Group)and endometrial coarse group(25patients).Matrix metalloproteinase-3(MMP-3)levels of all the subjects were measured by enzyme-linked immunosorbent assay(as basal level).All the subjects were followed up for one year to observe cerebral infarction events.Serum MMP-3 levels of each group,and the basic serum MMP-3 levels were compared among patients who were attacked or relapsed cerebral ischemic with those who had not been attack cerebral ischemic during this period of time.Results 5 patients in the cerebral infarction group had relapse (5%),2 patients in the CCCI group were attacked by cerebral ischemic(2%),and no one in the normal control group was attacked by cerebral ischemic.Serum MMP-3 levels in the acute cerebral infarction group were significantly higher than CCCI group,and both groups were significantly higher than normal control group (P <0.05).The basic serum MMP-3 levels in all patients who were attacked by cerebral ischemic were significantly higher than those who had not been attack by cerebral ischemic during this period of time(P <0.05).The serum MMP-3 levels of the unstable plaque group were significantly higher than stable plaque group.And both groups were significantly higher than endometrial coarse group(P <0.05).Conclusions Elevated levels of matrix metalloproteinase-3(MMP-3)might have something with the stability of carotid atherosclerotic plaque,and participate the attack and the relapse of acute cerebral infarction.Determination of MMP-3 might be used to predict the attack and relapse of acute cerebral infarction.
7.Determination of residual aluminium Ion in Huoxiang Zhengqi pellets by GFAAS with EDTA complexation extraction.
Xue-Na WANG ; Cong-Cong RAN ; Qing-Lian LI ; Chao-Hui DU ; Ye JIANG
China Journal of Chinese Materia Medica 2015;40(12):2345-2348
To establish an EDTA complexation extraction pretreatment combining with GFAAS method for the determination of residual aluminium ion in Huoxiang zhengqi pellets without digestive treatment, systematical investigation was made on sample preparation, and EDTA was used for the complexation extraction of residual aluminium ion in samples. The pH, concentration and volume of extraction solution, the temperature and time of microwave extraction, and graphite furnace temperature program were investigated. The results were compared with the microwave digestion. It was showed that, 0.1 g of sample weight was added in 20 mL 0.05 mol x L(-1) EDTA solution (pH 3.5), followed by heating at 150 degrees C for 10 min in the microwave extraction device. The determination of GFAAS was performed at optimized detection wavelength (257.4 nm) as well as graphite furnace temperature program, the detection limits and quantification limits were 2.37 μg x L(-1) and 7.89 μg x L(-1), respectively. The precision (RSD) was less than 2.3%. The average recovery was 96.9% -101%. The present method is easy, rapid and accurate for the determination of residual aluminium ion in Huoxiang zhengqi pellets.
Aluminum
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chemistry
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isolation & purification
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Drug Contamination
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Drugs, Chinese Herbal
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chemistry
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Edetic Acid
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chemistry
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Graphite
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chemistry
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Spectrophotometry, Atomic
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methods
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Temperature
9.The effect of the PXh haplotype combining estrogen receptor gene Xba Ⅰ, Pvu Ⅱ polymorphisms and osteocaicin gene Hind Ⅲ polymorphism on bone mineral density in postmenopausal women
Jirong GE ; Shengqiang LI ; Ke CHEN ; Yulian LAI ; Lian XUE ; Juan CHEN ; Lihua XIE
Chinese Journal of Geriatrics 2009;28(3):205-209
Objective To investigate the correlation between bone mineral density(BMD)and the PXh haplotype combining estrogen receptor (ER) gene Xba Ⅰ , Pvu Ⅱ polymorphisms and osteocalcin gene Hind Ⅲ polymorphism in postmenopausal women.Methods In 307 subjects,the BMD of lumbar vertebrae and proximal femur were measured by dual energy X-ray absorptiometry and the Xba Ⅰ and Pvu Ⅱ polymorphisms of ER gene and the Hind Ⅲ potymorphism of osteocalcin gene were detected by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP).Results (1)The BMD of greater trochanter was significantly lower in XX genotype group than in xx genotype group ( P<0.05).The BMD of femoral neck, greater trochanter and Ward's triangle were lower in Xx genotype group[(0.695±0.087)g/cm2 , (0.592±0.106)g/cm2, (0.500±0.115) g/cm2] and X allele group[(0.697±0.088)g/cm2 , (0.594±0.105)g/cm2, (0.505±0.123)g/cm2] than in xx genotype group[(0.737±0.108) g/cm2,(0.653±0.119)g/cm2 ,(0.554±0.130)g/cM2] and non-X allele group[(0.737 ± 0.108) g/CM2, (0.653 ± 0.119) g/cm2 , (0.554 ± 0.130) g/cm2] ,respectively (all P<0.05 ).(2)The BMD of Ward's triangle was lower in PP genotype group and P allele group than in pp genotype group and non-P allele group (P<0.05).(3)The BMD of femoral neck, greater trochanter and Ward's triangle were lower in hh genotype group and h allele group than in I-IH genotype group, and were lower in non-h allele group than in HH genotype group(all P<0.05).(4)Women carrying PX, PXh haplotypes combining ER gene and osteocalcin gene had lower BMD at femoral neck than those not carrying PX,not carrying PXh haplotypes, respectively (all P<0.05).ConclusionsER gene(Xba Ⅰ) polymorphism and osteocalein gene(Hind Ⅲ) polymorphism are associated with BMD in postmenopausal women.The presence of X allele or h allele shows negative influence on the BMD of postmenopausal women.The PXh haplotype is a suitable genetic marker of postmenopausal women osteoporosis in Fuzhou area.
10.Determination of 10 mycotoxin contaminants in Panax notoginseng by ultra performance liquid chromatography-tandem mass spectrometry.
Yong CHEN ; Chong-jun CHEN ; Jin LI ; Lian-jun LUAN ; Xue-song LIU ; Yong-jiang WU
Acta Pharmaceutica Sinica 2015;50(1):81-85
To ensure the quality and safety of Panax notoginseng, a method for the simultaneous determination of 10 mycotoxins in Panax notoginseng was developed using ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The sample was extracted with acetonitrile and purified by HLB multifunction cleanup column. The separation was performed on a Phenomenex Kinetex XB-C18 column by gradient elution using methanol and 5 mmol·L(-1) ammonium acetate as mobile phase. The targeted compounds were detected in MRM mode by mass spectrometry with electrospray ionization (ESI) source operated in both positive and negative ionization modes. The linear relationships of the 10 mycotoxins were good in their respective linear ranges. The correlation coefficients (r) ranged from 0.9981 to 1.0000. The LOQs of the 10 mycotoxins were between 0.15 and 8.6 μg·kg(-1). The average recoveries ranged from 73.8% to 107.0% with relative standard deviations (RSDs) of 0.10%-10.9%. The results demonstrated that the proposed method was sensitive and accurate, and suitable for the mycotoxins quantification in Panax notoginseng.
Chromatography, High Pressure Liquid
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Chromatography, Liquid
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Drug Contamination
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Mycotoxins
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analysis
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Panax notoginseng
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chemistry
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Tandem Mass Spectrometry