1.Culture of cryopreserved mouse spermatogonial stem cells.
Lian-jun LI ; De-xue LI ; Xue-ming ZHANG
Chinese Journal of Applied Physiology 2004;20(2):145-208
Animals
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Cell Culture Techniques
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methods
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Cells, Cultured
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Cryopreservation
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Male
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Mice
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Spermatogonia
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cytology
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Stem Cells
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cytology
4. Research progress of nlrp3 inflammasome in diabetic cardiomyopathy
Chinese Pharmacological Bulletin 2019;35(8):1051-1054
Diabetic cardiomyopathy( DCM) is one of the important cardiovascular complications of diabetes mellitus. Metabolic disorders, mitochondrial damage, cardiomyocyte apoptosis and autophagy are involved in the development of DCM. Recent research demonstrates that NLRP3 inflammasome plays a vital role in the pathogenesis of DCM, and has emerged as a potential tar get for the treatment of DCM. In this article, research progress in NLRP3 inflammasome and DCM has been reviewed, aiming to provide novel therapeutic idea for the treatment of DCM.
5. Activity regulation mechanisms of protein phosphatase 2A and its role in Alzheimer’s disease
Journal of International Pharmaceutical Research 2016;43(1):39-43
Protein phosphatase 2APP2Ais the main serine/threonine protein phosphatase of the brain, and it is involved in many physiological and pathological processes in the cell. Particularly in the pathogenesis of Alzheimer’s diseaseAD, PP2A is closely related to the main pathological features of AD. Deregulation of PP2A enzymes correlates with increased tau phosphorylation, the formation of amyloid precursor protein, and the missing of neurons. PP2A as the target of drug development may bring new hope for the treatment of AD. This review introduces the structure and activity regulation of PP2A, and the role of PP2A in AD.
6.The relationship among the matrix metalloproteinase-3,carotid artery plaque and ischemic cerebrovascular disease
Dongfang LI ; Junlin JI ; Guanglai LI ; Guofang XUE ; Xuejun XIE ; Yuheng PEI ; Xia LIAN
Journal of Chinese Physician 2011;13(3):302-304
Objective To investigate the relationship between the matrix metalloproteinase-3(MMP-3)and the stability of carotid artery plaque,and explore MMP-3's prediction role on the attack and relapse of acute ischemic cerebrovascular events.Methods 100 patients with the first ever acute cerebral infarction,100 patients with chronic cerebral circulation insufficiency(CCCI)and 40 persons without cerebrovascular diseases were enrolled in this study.According to the carotid ultrasound examination,100 cerebral infarction patients were divided into three subgroup: unstable plaque group(45 patients,mixed plaque,soft plaque),stable plaque group(35 patients,plaque Group)and endometrial coarse group(25patients).Matrix metalloproteinase-3(MMP-3)levels of all the subjects were measured by enzyme-linked immunosorbent assay(as basal level).All the subjects were followed up for one year to observe cerebral infarction events.Serum MMP-3 levels of each group,and the basic serum MMP-3 levels were compared among patients who were attacked or relapsed cerebral ischemic with those who had not been attack cerebral ischemic during this period of time.Results 5 patients in the cerebral infarction group had relapse (5%),2 patients in the CCCI group were attacked by cerebral ischemic(2%),and no one in the normal control group was attacked by cerebral ischemic.Serum MMP-3 levels in the acute cerebral infarction group were significantly higher than CCCI group,and both groups were significantly higher than normal control group (P <0.05).The basic serum MMP-3 levels in all patients who were attacked by cerebral ischemic were significantly higher than those who had not been attack by cerebral ischemic during this period of time(P <0.05).The serum MMP-3 levels of the unstable plaque group were significantly higher than stable plaque group.And both groups were significantly higher than endometrial coarse group(P <0.05).Conclusions Elevated levels of matrix metalloproteinase-3(MMP-3)might have something with the stability of carotid atherosclerotic plaque,and participate the attack and the relapse of acute cerebral infarction.Determination of MMP-3 might be used to predict the attack and relapse of acute cerebral infarction.
7.Isolation and identification of pathogenic bacterium which caused hemorrhagic diarrhea in newborn swine
Hongtao JIN ; Zhiping XIA ; Dan LI ; Huiliang XUE ; Jun LIU ; Hai LIAN
Chinese Journal of Zoonoses 2010;(3):209-210,214
The Escherichia coli (E.coli) strain from intestinal tract of died newborn swine was isolated and cultured.Preliminary identification of the isolated strain was conducted by conventional biochemical tests,and the molecular biology detections of toxicity gene and typing gene were completed by multi-PCR.Stable toxin and heat-labile enterotoxin genes of E.coli were detected from the isolated strain.By amplifying and sequencing bacterial 16s rDNA and fliC gene,the isolated strain was identified as H10 by Blast analysis.The homology of strain H10 was 99% with bacterial 16s rDNA gene and 98% with fliC gene.
8.Influence of Glucocorticoid on Bone Metabolism in Children with Tubercular Meningitis
feng-lian, ZHU ; gui-ling, FU ; jing, LI ; hui-yin, QIAN ; xue-peng, GUO
Journal of Applied Clinical Pediatrics 2004;0(08):-
0.05).The serum PICP of tubercular meningitis children after 4 neeks glucocorticoid therapy (108.85?46.13) ?g/L was significantly lower than that in control group((154.38)?47.98) ?g/L and glucocorticoid- pretreatment (152.99?44.78) ?g/L (P
9.Spindle cell variants of diffuse large B cell lymphoma: report of 2 cases.
Jun-chen WANG ; Da-ren SHI ; Xue-lian FU ; Chang-li LU ; Wan-ping LU
Chinese Journal of Pathology 2005;34(1):55-56
Antineoplastic Combined Chemotherapy Protocols
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therapeutic use
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Breast
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pathology
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Breast Neoplasms
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drug therapy
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pathology
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Cyclophosphamide
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therapeutic use
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Diagnosis, Differential
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Doxorubicin
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therapeutic use
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Female
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Follow-Up Studies
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Humans
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Lymphoma, B-Cell
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drug therapy
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pathology
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Lymphoma, Large B-Cell, Diffuse
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drug therapy
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pathology
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Male
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Middle Aged
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Prednisone
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therapeutic use
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Sarcoma
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pathology
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Spleen
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pathology
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Splenic Neoplasms
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drug therapy
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pathology
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Vincristine
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therapeutic use
10.Determination of residual aluminium Ion in Huoxiang Zhengqi pellets by GFAAS with EDTA complexation extraction.
Xue-Na WANG ; Cong-Cong RAN ; Qing-Lian LI ; Chao-Hui DU ; Ye JIANG
China Journal of Chinese Materia Medica 2015;40(12):2345-2348
To establish an EDTA complexation extraction pretreatment combining with GFAAS method for the determination of residual aluminium ion in Huoxiang zhengqi pellets without digestive treatment, systematical investigation was made on sample preparation, and EDTA was used for the complexation extraction of residual aluminium ion in samples. The pH, concentration and volume of extraction solution, the temperature and time of microwave extraction, and graphite furnace temperature program were investigated. The results were compared with the microwave digestion. It was showed that, 0.1 g of sample weight was added in 20 mL 0.05 mol x L(-1) EDTA solution (pH 3.5), followed by heating at 150 degrees C for 10 min in the microwave extraction device. The determination of GFAAS was performed at optimized detection wavelength (257.4 nm) as well as graphite furnace temperature program, the detection limits and quantification limits were 2.37 μg x L(-1) and 7.89 μg x L(-1), respectively. The precision (RSD) was less than 2.3%. The average recovery was 96.9% -101%. The present method is easy, rapid and accurate for the determination of residual aluminium ion in Huoxiang zhengqi pellets.
Aluminum
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chemistry
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isolation & purification
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Drug Contamination
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Drugs, Chinese Herbal
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chemistry
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Edetic Acid
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chemistry
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Graphite
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chemistry
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Spectrophotometry, Atomic
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methods
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Temperature