Objective To detect the expression of YB-1 and to research the relationship in the occurrence and development in the cervical squamous carcinoma(SCC) tissues.Methods Immunohistochemistry(IHC) envision was used to detect the expression of chronic cervicitis,CIN Ⅰ,CIN Ⅱ-Ⅲ and SCC.The relationship of YB-1 in the clinical pathological parameters of SCC were analyzed.Results YB-1 was mainly located in the nucleus in squamous cell,sometimes in the cytoplasm.The YB-1 protein did not expression in chronic cervicitis.In CIN Ⅰ,CIN Ⅱ-lⅢ and SCC,the positive expression had a gradual increasing trend.The expression of YB-1 was satistically significant in four groups (P＜0.05).The chronic cervicitis group,CIN Ⅰ groupCIN Ⅱ-Ⅲ group compared with the SCC group restivelly,the difference was statistically significant (P＜0.05).From spearman rank correlation analysis:the expression of YB-1 was positively correlated with the degree of cervical lesions (P＜ 0.05).In the cervical squamous carcinoma group,the expression of YB-1 was not associated with clinical pathological index of SCC patients(P＞0.05).Conclusion The change of the quantity of YB-1 protein is closely related to cervical squamous cell carcinoma.

Objective To investigate risk factors of chronic renal failure in IgA nephropathy.Methods A total of 384 cases of patients with IgA nephropathy from the First Affiliated Hospital of Guangxi University in Guangxi during 1997—2006 were divided into renal function normal group(320 cases) as the contrast and renal dysfunction group(64 cases) according to serum creatinine level whether beyond 133 ?mol/L,clinical and pathological materials were contrasted,stepwise multiple variance regression analysis was adopted to find the serum creatinine related factors.Results Significant difference was found between the two groups in rates of maleness,dropsy,hypertension,proteinuria,which were remarkably increased in the renal dysfunction group(P0.05).The scores of glomeruli index,interstitial index,blood vessel index,sclerotic index and so on were remarkably higher in the renal dysfunction group(P

Critical Illness ; therapy ; Fluid Therapy ; Humans

Objective: To evaluate the influence of acute moderate hemodilution on the dose-response and timecourse of effect of vecuronium. Method:Sixty patients. ASA grade Ⅰ,aged 17-45 years,scheduled for elective plastic surgery were included in the study,of which,thirty patients underwent hemodilution during surgery and thirty patients did not receive hemodilution as controls. General anesthesia was maintained with 60% nitrous oxide in oxygen,and further increments of thiopental 2 mg/kg or fentanyl 2?g/kg as required. After anesthesia was stable,the status of acute moderate hemodilution was developed by drainage o{ venous blood and intravenous infusion of lactated Ringer'ssolution, 6% dextran and.gelofusine,during which the level of hemoatocrit dropped from 45.7% to 26.2%. Neuromuscular function was assessed by TOF stimulation of accelerometry with the percentage depression of T_1 response used as the study parameter. The dose-response relationships of vecuronium were determined with the cumulative dose-response technique. Result:During hemodilution the dose-response curve of vecuronium was parallelly shifted to the left. Compared to the control patients,ED_(50),ED_(90) and ED_(95) of vecuronium in the hemodilution patients were decreased by 22%,18% and 17%, respectively. Following an intravenous administration of total dose of vecuronium 80?g/kg, vecuronium-induced neuromuscular block was significantly longer in the hemodilution patients than in the control patients. Conclusion:Acute moderate hemodilution could significantly enhance the neuromuscular blocking effect of vecuronium and prolong its duration of action.

Objective To observe the clinical efficacy of filiform fire-needle therapy plus mild moxibustion in treating flat warts.Method Ninety patients with flat warts were randomized into group A, group B and group C, 30 cases in each group. Group A was intervened by filiform fire-needle therapy plus mild moxibustion, group B was intervened by filiform fire-needle therapy alone and group C by mild moxibustion alone. The three groups all received treatment 3 times a week, 2 weeks as a treatment course, for 2 courses in total. The clinical efficacies of the three groups were compared.Result In group A, 12 subjects were recovered, 13 showed improved, and 5 cases failed; in group B, 7 subjects were recovered, 12 showed improved, and 11 failed; in group C, 5 cases showed recovered, 10 were improved, and 15 failed. The total effective rates were respectively 83.3% in group A, 63.3% in group B, and 50.0% in group C, and the total effective rate in group A was significantly higher than that in group B and C (P<0.05). Conclusion Filiform fire-needle therapy plus mild moxibustion can significantly help the skin rashes subside in treating flat warts, and the therapeutic efficacy is obviously superior to that of either of the two methods used alone.

Objective To investigate the impact of continued ambulatory peritoneal dialysis (CAPD)for 1 month,thus to provide effective therapy to control the symptoms of uremia in early stage. Methods A total of 129 nephrotic patients in final stage were treated with CAPD ,dialysis adequacy were assessed after 1 month of CAPD. Complications and biochemical indicators were compared between before and after 1 month of CAPD. Results The dialysis adequacy was good at the end of 1 month of CAPD. Compared to before CAPD,The prevalence of edema after 1 month of CAPD significantly decreased compared to before CAPD (7.8%vs. 24.8% ,χ2 = 13.765, P ＜ 0.05 ). After CAPD gastrointestinal, symptom, such as nausea and vomit significantly decreased from 66.7% to 6. 2% ( χ2 = 101. 821, P ＜ 0. 05 ). Itch of skin significantly decreased from 22. 5% before CAPD to 6. 2% after CAPD(χ2 = 13.914,P ＜0. 05) . Hemoglobin increased significantly from (79. 10 ± 17.13 ) g/L to (96. 50 ± 18. 69 ) g/L after CAPD ( t = - 6. 333, P ＜ 0. 01 ), serum calcium was sisilar, ( 1.99 ± 0.30) mmol/L and (2.07 ± 0. 20) mmol/L at before and after CAPD respectively ( t = -1. 920,P ＞0. 05). Albumin was (30. 62 ±5.24) g/L before CAPD and after CAPD(31.84 ±5.64) g/L ,with no significant difference ( t= - 0.333, P ＞ 0. 05 ) . Serum inorganic phosphorus, kalemia, urea nitrogen and creatinine concentration significantly decreased from ( 2. 06 ± 0. 54 ) mmol/L, ( 4.30 ±: 0. 68 ) mmol/L, 22. 00( 15.87,30.03 ) mmol/L and 864. 00 ( 733.00,1046. 25 ) μmol/L to ( 1.72 ± 0. 52) mmol/L, ( 3.84 ± 0.47 )mmol/L , 17.00 ( 13.91,20. 91 ) mmol/L and 777. 50 ( 627.00, 1047.75 ) μnol/L, respectively ( t = 3.284,4. 669, Z = - 3.717 and - 2. 408, respectively,Ps ＜ 0. 01 or 0. 05 ).. The level of serum PTH increased slightly from [ 184. 80 ( 114. 21,369. 77) ng/L to 226. 26 ( 124. 22,335.92 ) ng/L, but the difference was not significant ( Z = - 0. 597, P ＞ 0. 05 ). Conclusion CAPD had significant effect in early stage of dialysis with good dialysis adequacy. Hypocalcemia and hyperphosphatemia can be improved. The levels of serum kalemia decreased. The iatients's quality of life significantly improved.

AIM:To investigate the effect and the mechanism of Zhibai Dihuang Pill(Radix Rehmanniae praeparata,Fructus Corni,Rhizoma Dioscoreae,Rhizoma Anemarrhenae,Cortex phellodendri Chinensis,etc.) on patients with hyperthyroidism. METHODS: Eighty-five hyperthyroid patients were randomly divided into two groups.The control group(40 cases) were treated with propylthiouracil,while the treatment group(45 cases) were treated with propylthiouracil and Zhibai Dihuang Pill;in the 12-week long treatment period,the heart rate,body weight,thyroid free FT_3、FT_4 and TSH and the serum level of FAA,resistin,adiponectin,leptin of patients in both groups were measured. RESULTS: After treatment,the heart rate,the serum of FT_3,FT_4 decreased and the body weight,TSH increased in both groups(P0.05).(CONCLUSION): Zhibai Dihuang Pill can improve the abnormal metabolism of sugar and fat in patients with hyperthyroidism by its actions on the serum level of FFA,resistin,adiponectin,leptin.

Objective To investigate prognosis of acute ischemic patients with internal carotid artery or middle cerebral artery occlusion and define predictors of clinical outcome.MethodsThe clinical and radiological data of 48 stroke patients who underwent magnetic resonance angiography (MRA) within 6 hours of symptom onset and visualized internal carotid artery or middle cerebral artery occlusion on early MRA were analyzed. 90 days after onset, modified Ranking Scale (mRS) scores were obtained and factors related with prognosis were defined by logistic regression analysis.ResultsOf 48 patients, 17 cases (35.4%) got good clinical outcome (mRS 0～2), 31 cases (64.6%) got poor clinical outcome (mRS 3～6) with 7 cases (14.6%) died. In multivariate logistic regression analysis, only large infarction ( OR=21.1, 95%CI 1.4～314.2, P=0.027) and hyperglycaemia ( OR=5.1, 95%CI 1.3～19.8, P=0.019) were independent predictors of poor outcome.ConclusionClinical outcomes of most acute stroke patients with internal carotid artery or middle cerebral artery occlusion are poor. Large infarction and hyperglycaemia are independent predictors of clinical outcome.

Objective To investigate the clinical features and prognosis of acute ischemic stroke patients with different infarction patterns.Methods87 patients who underwent diffusion weighted imaging (DWI) within 6 h after symptom onset and visualized acute cerebral infarcts within the middle cerebral artery territory were prospectively studied.According to DWI lesion patterns,the patients were divided into 6 groups.Before MR was performed and 24 h after onset,scores of National Institutes of Health Stroke Scale (NIHSS) were recorded.3 months after onset,scores of modified Ranking scale(mRS) were obtained.ResultsThe baseline NIHSS,infarction volume,occlusion of relevant internal carotid artery or middle cerebral artery and clinical outcome were significantly different among 6 groups(P<0.05).ConclusionDifferent infarction patterns of acute ischemic stroke were associated with clinical symptoms,vascular status,and clinical outcome,that may help to guide treatment and judge prognosis.

Objective: To observe the influence of sot alol on the QT dispersion in patients with atrioventricular accessory pathways u nderwent radiofrequency catheter ablation (RFCA). Methods: Thirt y-six patients were divided into 2 groups by random. One was the drug group(18 cases) treated by RFCA， and sotalol 160 mg was orally administered and intracar diac electrophysiological study was performed every 30 min for 5 times. Th e other group(control group, 18 cases) only treated by RFCA.QTd,QTcd and QTLcd w ere measured before and after RFCA. Results: There was no signif icant difference with QT dispersion before and after RFCA in control group. When compared with before RFCA, QTd in patients administered sotalol was (30.9 ±14.3) ms vs (24.7±9.6) ms; QTcd(33.7±17.1) ms vs (25.2±10.1) ms; QT Lcd(30.8±14.1)ms vs (25.6±19.4) ms (P<0.05). Conclusion: Sotalol can slightly lower QT dispersion, which is beneficial for preventing malignant ventricular arrthythmia. It is safe in RFCA in pateints with accessory pathway.