ObjectiveTo explore the multidrug resistance(MDR) reversal activity of a novel compound liposome doxorubicin(PLD) and its mechanism.MethodsMTT assay was used to evaluate MDR reversal activity of PLD in P-gp expressing tumor cell lines,KBv200 and MCF-7/ADR.ResultsPLD had a strong reversal in vivo activity,recognized the strong reversal activity than verapamil reversal activity,in 5.0μmol/L concentration of multi-drug resistant cell KBv200 increased sensitivity to vincristine of 45 times.KBv200 PLD-dependent increased in intracellular concentration of rhodamine accumulation(0,2.5,5.0,10μmol/L).Mainly to its cardiac toxicity,bone marrow suppression and hair loss and other side effects were significantly reduced.ConclusionPLD was an efficient modulator,mainly through the continuous accumulation to tumor tissue,tumor local drug concentration,enhanced anti-tumor activity,

AIM: To investigate whether ?-tyrosol could induce expression of DT-diaphorase (NQO 1) gene and inhibit proliferation of hepatoma cells and their relationship.METHODS: The DT-diaphorase activity, mRNA expression and cell proliferation were examined using direct measurement of DT-diaphorase from cells cultured in microtiter wells, semi-quantitative reverse transcription-PCR (RT-PCR) technique and crystal violet assay after treatment of SMMC-7721 cells with ?-tyrosol for 24 h. RESULTS: Treatment of SMMC-7721 cells with ?-tyrosol caused an increase in DT-diaphorase activity and DT-diaphorase mRNA expression in a dose-dependent manner. A statistically significant correlation between DT-diaphorase mRNA levels and enzyme activities was evident. In almost the same concentration range of 70 mg/L to 100 mg/L, ?-tyrosol resulted in a dose-dependent inhibition of cell proliferation which well inversely correlated with DT-diaphorase activity.CONCLUSION: ?-tyrosol can induce DT-diaphorase activity and enhance expression of its gene, which may contribute to antiproliferation of hepatoma cells caused by the same chemical.

OBJECTIVE: To improve the preparative method of boric acid ear drops.METHODS: Boric acid ear drops were prepared using ultrasonic wave rather than waterbath heating to accelerate the solution of boric acid,and a comparison was conducted between the ultrasonic method and the waterbath heating method.RESULTS: The prepared boric acid ear drops were colorless supernatant liquid,which met the standard stated by the "Stipulations of clinical preparations in Zhejiang Province" in tests,identification and content.By ultrasonic method,the time spent for preparing boric acid ear drops was shorter(29?4.2) min than by waterbath heating method(18?1.4) min.CONCLUSION: The improved preparative method for boric acid ear drops is simple and feasible and the quality of the preparation is stable and controllable.

Colorectal Neoplasms ; pathology ; Humans ; Neoplasm Staging

Endosonography ; Esophageal Neoplasms ; diagnostic imaging ; radiotherapy ; Humans ; Image Processing, Computer-Assisted ; Lymph Nodes ; diagnostic imaging ; pathology ; Positron-Emission Tomography ; Radiotherapy Dosage ; Radiotherapy Planning, Computer-Assisted ; methods ; Radiotherapy, Conformal ; Tomography, X-Ray Computed ; Tumor Burden

The present study reported that 5srRNA from reticulocytes of rabbit could pass into the nucleus of mouse myeloma cells SP2/0,and in the mean time DNA synthesis and cells division were markedly suppressed. In a separate experiment, the rRNA was extracted from embryonic liver and erythrocytes of rabbit or rat and analysed by agarose electrophoresis method. The result indicated that the amount of 5srRNA in various period of the development of erythrocyte is changed along with denucleation process. Thus it is likely that 5srRNA of mammalian erythrocyte plays a role in reversing malignant phenotype of tumor cells and promoting denucleation of mammalian erythrocyte through inhibiting DNA synthesis.

Exposure to thermal environment is one of the main concerns for manned space exploration. By focusing on the works performed on thermoregulation at microgravity or simulated microgravity, we endeavored to review the investigation on space thermal environmental physiology. First of all, the application of medical requirements for the crew module design from normal thermal comfort to accidental thermal emergencies in a space craft will be addressed. Then, alterations in the autonomic and behavioral temperature regulation caused by the effect of weightlessness both in space flight and its simulation on the ground are also discussed. Furthermore, countermeasures like exercise training, simulated natural ventilation, encouraged drink, etc., in the protection of thermoregulation during space flight is presented. Finally, the challenge of space thermal environment physiology faced in the future is figured out.
Aerospace Medicine ; Body Temperature Regulation ; Environment ; Exercise ; Humans ; Space Flight ; Weightlessness ; Weightlessness Simulation

Objective:To compare the antihypertensive effects of tuina at Taichong (LR 3),Neiguan (PC 6) and Qiaogong (Extra) to screen the best tuina protocol for primary hypertension due to liver-fire flaming-up.Methods:A total of 102 patients with primary hypertension due to liver-fire flaming-up were randomly divided into a Taichong (LR 3) group,a Neiguan (PC 6) group and a Qiaogong (Extra) group according to the random number table,with 34 cases in each group.Patients in the three groups received 4-week tuina treatment with the corresponding acupoint respectively.The blood pressure was recorded by benchtop mercury sphygmomanometer before tuina,immediately after tuina treatment,30 min and 60 min after tuina treatment with the patient in a supine position in a quiet treatment room.The total effective rate was observed.Results:The systolic and diastolic blood pressures of the three groups all decreased after treatment (all P＜0.05).The decrease of systolic and diastolic blood pressure at different time points after treatment in the Qiaogong (Extra) group and the Taichong (LR 3) group were better than those in the Neiguan (PC 6) group (all P＜0.05).Qiaogong (Extra) group had the highest total effective rate though there was no statistical difference in the total effective rate among the three groups (P＞0.05).Conclusion:Treating primary hypertension due to liver-fire flaming-up with tuina at Taichong (LR 3),Neiguan (PC 6) and Qiaogong (Extra) can reduce systolic and diastolic blood pressure respectively,among which Qiaogong (Extra) has the highest total effective rate.

The purification of kallikrein using CTAB/hexanol/octane reverse micelles extraction has been studied and optimized,under various aqueous pH values, ionic strength and species, CTAB concentration and co-surfactant concentration. The result shows that the extraction efficiency approaches 100%, and the activity recovery is more than 80%, the commercial enzyme specific activity is increased by 1.97 times and the crude enzyme activity is increased by 7.15 times, which from 31U/mg to 219U/mg,under the conditions of[CTAB]=0.02 mol/L, hexanol/octane (V/V)=1∶5, pH=9.0 and[KBr]=0.1 mol/L in forward extraction, pH=7.0 ,[KBr]=1.5 mol/L and 15% ethanol(V/V) in backward extraction. The result of purified kallikrein is examined by the SDS-PAGE analysis. Reverse micelles extraction is a potential technique for the application in the downstream biotechnological processes.

Objective:To establish an HPLC method for the determination of osthole and columbianadin in Wangbi tablets. Meth-ods:The column was an Agilent XDB C18(150 mm ×4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-water(49∶51)with gradient elution. The flow rate was 1. 0 ml·min-1 and the detection wavelength was at 330 nm. Results: The calibration curve was linear within the range of 0. 060-0. 597 μg(r=0. 999 9) for osthole and 0. 010-0. 102 μg(r=0. 999 9) for columbianadin. The aver-age recovery was 99. 12% and 98. 98%, respectively. Conclusion:The method is simple, practicable, accurate and rapid. It can be applied in the content determination of osthole and columbianadin in Wangbi tablets.