AIM: To investigate the effects of iptakalim hydrohloride on K_(ATP) mRNA expression in renal tissues of spontaneously hypertensive rats. METHODS: SHRs at the age of 12-week-old were treated with Ipt 1, 3, and 9 (mg?kg~(-1)?d~(-1)), benazepril 3 (mg?kg~(-1)?d~(-1)) once a day for 12 weeks, respectively. The same aged WKY rats were used as normal control. The effects of Ipt on BP and renal K_(ATP) mRNA expression were detected by RT-PCR. RESULTS: mRNA expression level of SUR2?Kir6.1 and Kir1.1 increased in SHR. After administration of 1, 3, and 9 (mg?kg~(-1)?d~(-1)) Ipt,the levels of BP were decreased,and the mRNA expression of Kir6.1 and Kir1.1 were decreased. But there was no change in mRNA expression of SUR2. In addition, there was no significantly difference of mRNA expression of Kir6.2 among the SHR groups and the WKY group. CONCLUSION: The renal protective effects of Ipt may be related to regulation of genes expression of Kir6.1 and Kir1.1.

In recent years,with the further study of bone marrow mesenchymal stem cell (BMSC),using BMSC transplantation for the treatment of ischemic cerebrovascular disease has become a research hotspot.However,there are still considerable controversies in the mechanism of action,transplanting time,transplantation path,and specific marker.

To explore the experience of the process of Tai Chi exercise.

Acute appendicitis is the most common general surgical problem encountered during pregnancy, which may be associated with serious maternal and /or fetal complications such as appendiceal perforation or premature delivery.Clinical presentation and imaging remains vital in the diagnosis of appendicitis.As a general rule,the clinical suspicion of acute appendicitis during pregnancy is an indication for an urgent surgical intervention.Appendectomy is the preferred treatment.Laparoscopic appendicectomy(LA)can also be performed safely and effectively in pregnant patients without bringing additional maternal complications.

Arterial Occlusive Diseases ; diagnosis ; etiology ; Coronary Disease ; complications ; diagnosis ; Humans ; Lower Extremity ; blood supply ; Male ; Middle Aged

ObjectiveThe purpose of this study was to evaluate the effect of prostaglandin E1 (PGE1) on blocking the development of acute respiratory distress syndrome (ARDS) induced by acid aspiration. MethodsTwenty new Zealand rabbits were used. Dilute HCl was instilled into right bronchus of the rabbits. The rabbits were then divided randomly into two groups: injury group and treatment group. In injury group ( n = 10) rabbits received no treatment except mechanical ventilation. In treatment group ( n = 10) immediately after acid instillation the rabbits received an intravenous bolus of PGE1 followed by a continuous infusion. Blood gas, airway pressure and dynamic and static compliance were measured before and after acid instillation. Blood samples were taken from artery for determination of 6-k-PGF1α, TXB2, NO2-/NO3- and ET-1. The animals were killed and the wet/dry lung weight (W/D) ratio and total protein of bronchoalveolar lavage fluid(BALF) of right lung were measured. Microscopic examination of the lung was done. ResultsIn treatment group PaO2 was significantly higher than that in injury group after acid instillation. Plasma 6-k-PGF1α and NO2-/NO3- levels were significantly higher in treatment group while plasma TXB2 and ET-1 levels were significantly lower. W/D ratio and TP of BALF of right lung were significantly lower in treatment group. The inflammatory changes were diffuse in injury group while in treatment group they were localized and less severe. Conclusions PGE1 can lessen severity of ARDS induced by acid aspiration. It may protect pulmonary vascular endothelial cells through maintaining the balance between PGI2 and TXA2 and that between NO and ET-1 .

The article presented the drugs supply management mode reform in place since 2009 in the one district of Shanghai.Authors analyzed and commented the implementation and outcomes,and studied the potentials of successful promotion nationwide.They hold that such a mode works to some extent in terms of reducing management costs of medical institutions,lowering drug purchase price,encouraging rational drug use of such institutions,and reforming the compensation manner for medical institutions.This mode,however,calls for an operating environment in need of not only funding support,but also new concepts and economic development strength of the society as a whole.This means that such a mode is only recommended in developed areas in China.

Objective To manage calcaneal fractures with an U-shaped external fixator combined with close manipulative reduction. Methods From February 1997 to November 2002, 31 patients with 34 calcaneal fractures were treated with self-designed U-shaped external fixator combined with close manipulative reduction. All patients were males aging from 7 to 50 years with an average of 32 years. The external fixation device consisted of two partial rings, three modular connecting rods and several pins. According to Essex-Lopresti classification, there were 10 extra-articular fractures without involving the subtalar joint, and 24 intra-articular fractures involving the subtalar joint. Two upper pins were inserted in cross through the distal tibia, and one of two lower pins was inserted laterally through the anterior part of calcaneus, the other was posterior into the calcaneous tuberosity or larger fragment connecting with Achilles tendon. After the manipulative reduction, the frames were applied. Results 31 calcaneal fractures were found with satisfactory reductions after manipulation. The widths of the heels were restored in certain extent; the heights of foot arch and calcaneus were resumed to normal; the B hler angle increased from 15? to 40? (mean, 25?). The duration of hospitalization was on an average of 7 days. The duration of external fixation was averagely 8 weeks. All patients were followed up with a mean period of 8 months (range, 6 to 12 months). All of the fractures healed well. No nonunion, delayed healing of bone or other complications was found. All patients resumed to walk on foot after 2 to 4.5 months. According to Fernandez scoring system, results were graded as excellent and good in 31(91%). Conclusion Using U-shaped external fixator combined with close manipulative reduction is a satisfying technique to treat calcaneal fractures. By ankle joint spanned traction and fixation, this technique overcomes displacement of fragments caused by contracture of tendo calcaneus, maintains width of heel and biodynamic anatomy of foot, avoids complications of wound caused by open reduction and internal fixation.

Objective To investigate the effects of nitric oxide (NO) inhalation on the expression of TNF-?,IL-8 and CD11b mRNA in lung tissue during acute respiratory distress syndrome (ARDS) induced by intravenous injection of endotoxin in dogs.Methods Twelve pure bred beagle dogs of both sexs weighing 8-12.5 kg were randomly divided into 2 groups: NO group received mechanical ventilation with NO inhalation (n = 6) and control group received only mechanical ventilation ( n = 6) . Sepsis and ARDS were induced by intravenous injection of endotoxin as described in detail in our previous paper. Hemodynamics and pulmonary oxygenation were monitored and shunt fraction was calculated. At the end of experiment the animals were sacrificed and lung tissue was obtained aseptically and stored in the liquid nitrogen at - 180℃ . The total RNA was extracted. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of TNF-?,IL-8 and CD11b mRNA. The total RNA was reversely transcripted to cDNA. Then the cDNA was amplified by PCR. The product of PCR was scanned by gel-image analysis system.?-action was used as internal control. Semi-quantitative method was adopted for measurement of TNF-? ,IL-8 and CD11b mRNA expression. Results The expression of TNF-?, IL-8 and CD11b mRNA in lung tissue was significantly decreased in NO group compared with those in control group.Conclusion NO inhalation reduces expression of TNF-?, IL-8 and CDllb mRNA in lung tissue during ARDS induced by intravenous endotoxin.

Objective To investigate the effect of propofol on apoptosis at different time points after focal cerebral ischemia-reperfusion (I/R) in a rat model of reversible middle cerebral artery occlusion (MCAO). Methods Sixty male SD rats weighing 240-260g were randomly divided into 2 groups ( n = 30 each): propofol group received intraperitoneal (i. p.) propofol 80 mg ?kg before MCAO and control group received equal volume of normal saline instead of propofol. The animals were anesthetized with 10% chloral hydrate 350mg?kg-1 i.p. . The right external, internal and common carotid artery were exposed. A 4-0 nylon thread with rounded tip was inserted via external carotid artery into internal carotid artery and threaded cranially until resistance was felt. MCAO was maintained for 90 min before reperfusion. The animals were killed and brains removed for detection of apoptotic neurons using TUNEL staining combined with electronic microscopic examination at 3 h, 6 h, 24 h, 3 days, 5 days and 7 days after reperfusion was started ( n = 5 at each time point in both groups) . Results In control group the ratio of apoptotic neurons peaked at 24 h of reperfusion and then gradually decreased; while the ratio of TUNEL positive neurons kept on increasing indicating a shift from apoptosis to necrosis after 24 h reperfusion. The ratio of TUNEL positive neurons and apoptosis were significantly lower in propofol group than in control between 6 h to 3 days after reperfusion was started. Conclusion Propofol pretreatment attenuates apoptosis induced by focal cerebral I/R and the maximum effect is reached and maintained between 6 h to 3 days of reperfusion.