Objective To explore the effect of hydrogen peroxide (H2O2) on the expression of human leukocyte antigen-G ( HLA-G) in placental trophoblasts in pregnant women with pre-eclampsia.Methods Forty pregnant women,delivered through cesarean section in the Department of Obstetrics of and Gynecology,the First Affiliated Hospital of Nanjing Medical University from October 2008 to October 2009,were enrolled,including 20 women with pre-eclampsia and 20 healthy gravidas (control group).Colorimetry and western blot were applied,respectively,to determine the level of H2O2 and the expression of HLA-G protein in placental tissues and the correlation between them were analyzed.After 24 hours of seeding,JEG-3 cells (the HLA-G positive cell line of choriocarcinoma) were divided into two groups:intervention group (exposure to 175 μmol/L H2O2) and control group (without H2O2).Immunofluorescence and western blot were used to investigate the expression of HLA-G protein in JEG-3 cells at 24 hours and 48 hours after incubation.Results (1 )The level of H2O2 in placenta in the pre-eclampsia group was significantly higher than that in control group[(105 ±13) nmol·mg-1·prot-1 vs (62 ± 18) nmol·mg-1 ·prot-1,P < 0.05].(2) The expression of HLA-G protein in placenta of the pre-eclampsia group was reduced by88%compared with that of the control (0.20 ± 0.08 vs 1.67 ± 0.65,P < 0.05).( 3) Negative correlation was found between HLA-G level and H2O2 expression in the placenta in both groups(r =-0.895,P =0.000).(4) Compared with the control group,the expression of HLA-G protein in JEG-3 cells,after 24 hours and 48 hours exposure to H2O2,reduced by 39% and 80%,respectively,(3.21 ±0.33 vs 1.95 ±0.25 and 0.65 ±0.08,P <0.05,respectively) and 67% reduction was detected from 24 hours to 48 hours of H2O2 exposure (P <0.05).Fluorescence microscope observed reduced expression of HLA-G in JEG-E cells in the intervention group at 48 hours compared to the control group (P<0.05).Conclusion High level of H2O2 could down-regulate HLA-G expression in the placental trophoblasts in pre-eclampsia which may be involved in the pathogenesis of pre-eclampsia.

Objective: To investigate the changes of alpha adrenergic receptors (α-AR) mRNA expression in heart and mesenteric artery in autonomic dysreflexia (AD) rats after spinal cord transection, so as to explore the possible mechanism of AD. Methods: The spinal cord of rats was exposed and the fourth thoracic spinal cord was transected; 4 weeks later, rats' rectum was stimulated by self-made catheter and those with a mean arterial blood pressure increased by more than 15 mmHg (1 mmHg=0.133 kPa) were chosen as AD group (n=16). Heart and mesenteric arteries along with their branches were harvested. mRNA expression of α1A-, α1B-, α1D-, α2A-, α2B- and α2C-AR was quantified by real time PCR and the result was compared with that in sham-operated group (the fourth thoracic spinal cord was exposed but not transected). Results: Compared with sham-opera ted group, rats in AD group had a lower expression of α1A-AR mRNA (P<0.05) and α1B-AR mRNA (P<0.01) in their heart, but the expression of α1D-AR mRNA had no obvious change. Also, rats in AD group had a higer expression of α1A-, α1D-, α2B-AR mRNA (all P<0.01) and α2C-AR mRNA (P<0.05) in their mesentric artery, but α1B- and α2A-AR mRNA expression had no obvious change. Conclusion: AD may occur in the chronic stage of spinal cord transection in rats. The abnormal expression of α1A- and α1B-AR mRNA in the heart and α1A-, α1D-, α2B- and α2C-AR mRNA in the mesenteric artery may be related to AD.

The aim of this paper is to investigate the effects of protocatechuic acid (PCA) on the expression of D2DR, iNOS, and TH in striatum and midbrain of Parkinson's disease (PD) mice induced by MPTP. C57BL brown mice were used as experimental animals and were injected with MPTP (25 mg/kg. d) for 7 d to build PD model mice. PCA (10 mg/kg. d) and Madopar (125 mg/kg. d) had been ip administered to the PD mice for the precaution and treatment at the first and lasted for 7 d. The expression of D2DR, iNOS, and TH in the striatum and midbrain of PD mice induced by MPTP was detected by Western blotting method. The results showed that iNOS expression increased while D2DR and TH expression decreased in the striatum and midbrain of MPTP-induced PD mice. However, PCA significantly decreased the expression of iNOS while obviously increased the expression of D2DR and TH in the striatum and midbrain of MPTP-induced PD mice. PCA has a neuroprotective effect on the PD mice induced by MPTP, and its protective effects on mice brain are achieved at least partly by increasing TH and D2DR expression while decreasing iNOS expression.

To investigate the genotype distributions of PLA1/PLA2 polymorphism in Chinese Han population from Beijing and Hebei Province and to study the correlation between the platelet membrane glycoprotein IIIa polymorphism and coronary heart disease (CHD) or CHD with blood-stasis syndrome.

Based on detailed analysis of the status of and problems in the use and management of implantable medical devices and equipment in clinical departments of hospital, we propose some clinically operable solving strategies and methods. It is important to further regulate the use and management of implantable medical devices for timely and safe use the devices in clinical departments.

0.05).[Conclusion]The factors causing reductions should be removed arthroscopically.Arthrocopy may be a useful micro-treatment method for DDH.

AIM:To investigate the genotype distributions of HPA-3 in Han people from Beijing and Hebei province,and to study the association of the platelet glycoprotein IIb polymorphism with coronary heart disease(CHD).METHODS:Two hundred and twelve patients with coronary heart disease and 106 healthy controls were enrolled in this case-control study.The number of occlusive coronary artery was performed in all subjects.The genotypes of HPA-3 were determined by TaqMan probe technology.RESULTS:In CHD patients(older than 45 years)carriers of HPA-3b were over-represented compared with healthy controls(P

Abstract Objective: To investigate the regulatory effect of PAF on early events in signal transduction during T-cell activation. Methods:T-cell activation was achieved by stimulation of human T cells with CD3 mAb or PMA/ionomycin in the presence or absence of PAF. T cell Pro-liferatilon was determined by 3 H-thymidine incorporation, IL-2 production was measured by MTT method, IL-2R(CD25)expression was evaluatedby flow cytometry and,PKC activity was assayed by the method described by Hauschildt. Results:PAF inhibited CD3 mAb-induced T-cell pro-liferation, IL-2 production and CD25 expression. AIthough it inhibited T-cell proliferation and IL-2 production induced by PMA/ionomycin, PAFfailed to inhibit IL-2 production induced by PMA/ionomycin.The translocation of PKC was also inhibited by PAF if T cells were activated byCD3mAb. Conclusion: PAF inhibits T-cell activation via its inhibitory effect on PKC activation. Its differential effect on IL-2 production suggeststhat PAF regulatory more early events in signal transduction such as the activation of phospholipase C ?-1.

Objective:To investigate the changes of plasma moderate molecule substance (MMS)level in pediatric patients with congenital heart disease during open heart surgery. Method: Siteen cases were studied. Blood samples were taken before anesthesia, after tracheal intubtion, after sternotomy. and rewarming during cardiopulmonary bypass, to measure the plasma MMS levels with ultraviolet absorption spectrophotometry. Result: As compared with that before anesthesia, the MMS levels after tracheal intubtion,sternotomy,and rewarming during cardiopulmonary bypass were significantly elevated(P

Background Scarring of surgical area,the most important factor,leads to the failure of glaucoma filtering surgeries.Therefore,more and more attentions are paid to the causes and process of scar formation.Objective This study was to compare the differences of proliferation and migrating abilities of fibroblasts between filtering bleb scar tissue and normal Tenon capsular tissue,and to investigate the inhibitory effects of miRNA-200a (miR-200a) on biological behavior of conjunctival fibroblasts.Methods Normal Tenon capsular tissue and filtering bleb scar tissue were collected during the strabismus surgery and glaucoma filtering surgery,respectively for the primarily culture of fibroblasts.The proliferation (absorbency,A) of the cells was assayed by cell counting kit-8 (CCK8) method;the relative migrating distance of the cells was measured by cell scratch test;and the relative expressions of transforming growth factor-β1 (TGF-β1)mRNA and miR-200a mRNA in the cells were detected by realtime fluorescence quantitative PCR.TGF-β1 mimic of 0,1,2 and 5 ng/ml was added in the medium of human normal Tenon capsular-derived fibroblasts (HTFs),and 0.00,0.25,0.50,1.00 μg/ml TGF-β1 inhibitor was added in the medium of human scarring-derived fibroblasts (HSFs) for 24 hours,respectively,and CCK8 was used to evaluate the proliferation of the cells.The relative migrating distance as well as the relative expressions of miR-200a mRNA were analyzed in the 2 ng/ml TGF-β1 mimic-or 1.00 μg/ml TGF-β1 inhibitor-treated cells.Results The primary conjunctival presented the spindle and star-like in shape with large body and oval nuclei.The cells showed the positive response for keratin and vimentin antibodies.The A values were 1.476±0.110 in the HSFs and 0.958±0.074 in the HTFs,with a significant difference between them (t =24.900,P=0.016).The relative expressions of TGF-β1 mRNA were significantly higher in the HSFs than those in the HTFs,and the relative expressions of miR-200a were evidently lower in the HSFs than those in the HTFs,showing significant differences between them (t =6.358,P =0.024;t=7.394,P =0.018).Compared with the 2 ng/ml TGF-β1 mimic-treated HTFs,the relative migrating distance increased,while the expression level of miR-200a mRNA was significantly reduced in the 2 ng/ml TGF-β1 mimictreated HSFs (all at P＜0.05);Compared with the 1.00 μg/ml TGF-β1 inhibitor-treated HTFs,the relative migrating distance decreased,but the expression level of miR-200a mRNA was significantly elevated in the 1.00 μg/ml TGF-β1 inhibitor-treated HSFs (all at P＜0.05).Conclusions The proliferation and migrating abilities are stronger in the HSFs than those in the HTFs,which probably is regulated by the expression of miR-200a in the cells.The miR-200a plays a negative feedback for the effect of TGF-β1 promoting proliferation and migration of fibroblasts.