Objective To explore the effect of recombined Human epidermal growth factor (rhEG) combined with alprostadilon Wnt/β-Catenin signal pathway in rats with diabetic ulcer. Methods Rats with diabetic ulcer were randomly divided into four groups :control group ,rhEG group ,epidermal group and combined treatment group. Ulcer skin was smeared by normalsaline in control group ,and by rhEG in rhEG group. Epidermal was administered from caudal vein in epidermal group.Combined treatment group was treated by rhEG and epidermal at the same time. The healing status were observed. The proteinand mRNA expression of Wnt-1 ,β-Catenin and GSK-3β were measured 14 days after treatment. Results The healing rates in combined treatment group were (9.76 ± 2.37 )% ,(35.74 ± 3.65 )% ,(51.37 ± 4.16)% and (84.42 ± 5.35 )% respectivelyin 6th , 10th and 14th day after treatment , which were significantly higher than in rhEG group and epidermal group (P<0.05). The mRNA levels of Wnt-1 ,β-Catenin and GSK-3βin combined treatment group were (1.42 ± 0.19) ,(1.56 ± 0.21) and (0.95 ± 0.15) after treatment for 14 days ,which were significantly higher than in rhEG group and epidermal group (P<0.05). Protein levels of Wnt-1 ,β-Catenin and GSK-3βin combined treatment group were (1.17 ± 0.16) , (1.38 ± 0.18 ) and (0.81 ± 0.13 ) after treatment for 14 days ,which were significantly higher than in rhEG group and epidermal group ( P < 0.05 ). Conclusion rhEG combined with epidermal can significantly accelerate the healing of diabetic ulcer ,and can regulatethe Wnt/β-Catenin signal pathway by increasing the expression of Wnt-1 ,β-Catenin and GSK-3β.

Object To investigate the effects of resveratrol (Res) on the proliferation and apoptosis of nasopharyngeal carcinoma cell line CNE-2Z. Methods IC 50 values were detected by MTT assay. The apoptosis was analyzed by flow cytometry (FCM), Hoechst 33258/PI fluorescence staining, and DNA agarose gel electrophoresis. Results After CNE-2Z cells were treated with different concentrations of Res for 24, 48, and 72 h, their IC 50 values were (109.2?7.5), (83.6?6.0), and (54.3?2.8) ?mol/L respectively (P

With the development of communication techniques,the satcom telemedicine system is being applied more and more widely. Satellite communication is a means of communication of the broadest transmission scope and the largest coverage.The establishment of the satcom medicine system has effectively solved such bottleneck problems as date transmission block and network interrupt,The present article reviews the development of the satcom telecommunication system at home and abroad,the composition and characteristics of telecommunication,the composition and function of the PLA VSAT satcom telemedicine system,and the trend of development.

Objective:To study whether caspase-6 was activated during resveratrol(Res)- induced apoptosis of nasopharyngeal carcinoma cells CNE-2Z. Methods:The cleavage of pro-caspase-6 was analyzed by Western-blot. TTie changes of caspase-6 mKNA was detected by semi-quantitative RT-PCR. The changes of caspase-6 activity was determined by colorimetric assay. Results: After CNE-2Z cells were treated with 0 (control), 25, 50, 100, 200 pmol/L Res for 24 h, respectively, the expression of pro-caspase-6 was decreased with concentration increasing. Caspase-6 active fragment P20(20 kD) appeared at 100 fjtmol/L and was increased at 200 (junol/L.The expression of caspase-6 mR-NA was increased in a concentration-dependent manner ( P

Objective:To determine the equilibrium solubility and the apparent oil/water partition coefficient of nebivolol hydrochloride to provide experimental basis for the development of new preparations.Methods:The concentration of nebivolol hydrochloride was determined by an HPLC method,and a saturated solution method and a shake-flask method were respectively applied to determine the equilibrium solubility and the apparent oil/water partition coefficient of nebivolol hydrochloride in water,0.1 mol·L-1 HCl solution and phosphate buffer solution with different pH values(pH2.0,pH6.8,pH7.4 and pH8.0).Results:At (37±0.5)℃,the equilibrium solubility of nebivolol hydrochloride in water and in 0.1 mol·L-1 HCl solution was 722.53 μg·ml-1and 56.07μg·ml-1,respectively.The apparent oil/water partition coefficient (Log P) of nebivolol hydrochloride was 1.17 and 1.32,respectively.Within the pH range of 2.0-7.4,with the increase of pH value, the equilibrium solubility and the Log P decreased and increased,respectively,while pH value increased from 7.4 to 8.0,the equilibrium solubility of nebivolol hydrochloride increased and Log P decreased.Conclusion:The method is accurate and reliable.Nebivolol hydrochloride has poor water solubility,and the equilibrium solubility and the Log P are both influenced by pH values.

Objective To observe spinal cord neurotoxicity injury induced by mixture of bupiv-acaine-lidocaine compared with mixture of ropivacaine-lidocaine.Methods Forty-eight SD rats,weig-hing 250-300 g,were randomly allocated into 6 groups (n =8):NS group (group N),1.065% bupi-vacaine group (group B),1.5% ropivacaine group (group R),5% lidocaine group (group L),mix-ture group of 5% lidocaine and 1.065% bupivacaine (group LB)or mixture group of 5% lidocaine and 1.5% ropivacaine (group LR).The rats were given intrathecal cathetering after anesthesia.Tail-flick test (TFL)and the mechanical withdrawal threshold (MWT)of rats in each group were meas-ured 1 d before operation (the baseline values)and 1 d,2 d,3 d and 4 d after intrathecal administra-tion.The movement of rat double hind legs were observed at 10 min,30 min,1 h,2 h,3 h and 4 h and 1 d,2 d,3 d and 4 d after intrathecal administration,and the changes of recovery time of lower limbs were recorded.The rats were sacrificed 4 days postoperatively followed by observation on the indexes mentioned above,and spinal cord and cauda equine were taken for staining.Then the patho-logical changes of spinal cord were observed under light and electron microscope,and caudal nerve was rated for pathological injury.Results TFLs of group LR were obviously longer than those of group N,L,B,R,LB 1-4 d after intrathecal administration (P <0.05);Compared with the group N,there was no statistical difference of TFL in the group L,B,R,LB;there was no statistically sig-nificant difference of rat MWT in comparison among the six groups at each time point.There was no statistically significant difference among six groups in recovery time of movement after intrathecal ad-ministration;and the pathological injury scores were obviously higher in group LR than that of group N,L,B,R,LB (P <0.05),respectively.Compared with the group N,there was no statistical difference of the pathological injury scores in the group L,B,R and LB,respectively.Under light microscopy observa-tion:the structure of spinal cord was normal in group N,B and R.In group L and LB,there was focal ede-ma of neurofibers.In group LR,there was edema of neurofibers or demyelination,accompanied with white mass edema in posterior horn of spinal cord.Under electron microscope,only slight edema of medullated fi-bers and unmyelinated nerve fiber was seen in group B and group R;part of the medullated fibers appeared sparse focal lamellar structure in group L and group LB;swelling and degeneration of neuraxon,separation of neuraxon from myelin sheath and disappearance of unmyelinated nerve fiber were seen in group LR. Conclusion The mixture of 5% lidocaine with 1.5% ropivacaine is more neurotoxic to spinal cord than the single administration of lidocaine or ropivacaine,moreover it is not obviously changed compared with the mixture of lidocaine with equipotent bupivacaine.

The current situation of the satellite-based distant medical education, the distant hygienic education was introduced. We analysed the characteristic and advantage and indicated the importance and necessity of the satellite-based distant medical education.

Objective To investigate the effect of HgCl2 and drugs on the expression of calcitonin gene related peptide(CGRP) in the spinal cord and dorsal root ganglion(DRG).Methods Forty male SD rats(180-220 g) were randomly divided into 7 groups,5 in each treated group,10 in the control.Group A(high HgCl2),B(high HgCl2 +DMPS),C(high HgCl2+DMPS+ carbamzepine) were administrated with HgCl2 at the dose of 17 mg/kg(1/4 LD50),group D(low HgCl2),E(low HgCl2+DMPS),F(low HgCl2+NS) at the dose of 8.5 mg/kg(1/8 LD50),by gavage,once a day,the control group treated with normal saline(NS) in the same way.The sub-acute mercury poisoning models were developed within 18-22 days.Then five rats of control group and group A and D were killed and group B and E were treated with the chelator(DMPS) by intraperitoneal injection at the dose of 28 mg/kg for 2 periods.Group C was given DMPS+ carbamzepine and group F was given NS only.The carbamzepine was used at the dose of 16.82 mg/kg by gavage daily for 14 days.The spinal cord(L5-6) and the DRG of L5 and L6 were collected after the treatment finished.The immunohistochemistry SABC way was used to detect CGRP.Results The CGRP level in spinal cord and DRG of the rats exposed to HgCl2 increased significantly compared with the control group(P

25, were received iNO at a concentration of 3~15 ppm. Results Before iNO, mean FiO 2 was 0.80?0.15, SpO 2 was (0.74?0.13) and OI was 26?16. At 3 and 24 hours after iNO, FiO 2 decreased to 0.72?0.15 and 0.52?0.11 (both P

Objective To study the adverse effect of mercury on the tibial nerves of rats. Methods Seventy SD rats (35 females, 35 males, weighed 160-200 g) were randomly divided into 4 groups. 40 rats in group 1 were used to test the acute toxicity of HgCl2, 10 rats in group 2 were given HgCl2 by gavage at 17 mg/kg(1/4 LD50) daily, in group 3 the dose was 8.5 mg/kg(1/8 LD50), 10 rats in group 4 were treated with 0.9% saline 2 ml by gavage daily, the experimental duration was (20?4) days. When the model had been developed, all the male rats were killed and the female rats in group 2 and 3 were given the chelator(DMPS) by intraperitoneal injection at 28 mg/kg for 2 periods of treatment, then were killed and the tibial nerves were examined with the electronic microscope. Results The oral LD50 of HgCl2 was 68.1 mg/kg. Seven rats in group 2 (7/10) were poisoned and two of them got pain manifestation. Six rats in group 3(6/10) were poisoned. Myelinoclasis and the changed axons were found in the tibial nerves of poisoned rats and it was more serious in the rats with pain. The chelator(DMPS) did not relieve the change. Conclusion Sub-acute poisoning of HgCl2 can induce degeneration of tibial nerves in SD rats .