Objective To investigate and evaluate the comparison results of five items of hepatitis B in ELISA by using water bath box and rapid incubation .Methods 92 patients with hepatitis B in our hospital from June 2012 to June 2013 were randomly se‐lected and 184 cases of healthy physical examination were randomly selected as the controls .The water bath and rapid incubation were adopted to conduct ELISA for simultaneously detecting 5 items of hepatitis B in samples .Results The results of two groups had higher positive coincidence rate .The average OD value of specimens in the water bath was higher .But the specimen S/CO value in the fast average incubation was higher ,its co value was higher than that of the water bath .Conclusion The rapid incubation can be introduced for improving the work efficiency in the daily work ,but the specimen with weak reactiveresult needs to be re‐de‐tected by using the water bath and making records .The fast incubation device is best for only use of the HBsAg reaction ,because the other four reaction process time is shorter ,and there are some non‐conformity results .

Objective To investigate the effect of orthopedic distractor assisted reduction and percutaneous plate implantation in the treatment of distal tibial fractures.Methods 120 patients with distal tibial fractures treated in Wendeng orthopedic hospital of Shandong Province from January 2019 to January 2021 were randomly divided into two groups:the control group(60 cases,percutaneous plate implantation and internal fixation under traditional manual reduction)and the observation group(60 cases,orthopedic distractor assisted manual reduction and percutaneous plate implantation and internal fixation).The treatment effect,intraoperative indexes and postoperative fracture recovery of the two groups were compared,and the complications of the two groups were recorded and compared.Results After treatment,there was no significant difference in the excellent and good rate of fracture healing in the observation group(P＞0.05);The operation time[(72.56±27.54)min]and fracture healing time[(16.45±4.59)w]of the observation group were shorter than those of the control group[(89.94±28.20)min and(21.15±4.54)w],and the amount of intraoperative bleeding in the observation group[(82.27±20.14)ml]was less than that of the control group[(90.12±21.48)ml],the differences were statistically significant(P＜0.05);12 months after operation,the knee joint score of American Special Surgery Hospital(HSS)and ankle hindfoot score of American ankle Orthopedic Association(AOFAS)in the observation group[(85.24±7.52)and(84.58±7.29)]were lower than those in the control group[(74.45±7.64)and(74.56±6.38)](P＜0.05).There was no significant difference in the total incidence of complications between the two groups(P＞0.05).Conclusion Orthopedic distractor assisted reduction and percutaneous steel plate implantation and internal fixation have good curative effect,which can effectively reduce the amount of intraoperative bleeding,shorten the operation time,and have a good recovery of foot and ankle function after operation.

Objective To explore the efficacy of microvascular technology combined with perforator flap repair in the treatment of soft tissue defects in hand injuries.Method 98 patients with hand injury soft tissue defects admitted to our hospital from August 2018 to February 2023 were selected as the study subjects.The patients were randomly divided into a control group and an observation group using a random number table method,with 49 cases in each group.The control group was treated with thoracoabdominal pedicle flap repair,while the observation group was treated with microvascular technology combined with perforator flap repair.Compare the efficacy,visual analogue scale(VAS)score,histopathological score,total finger mobility(TAM)score,sensory function grading,and comprehensive quality of life assessment questionnaire 74(GQOLI-74)score between two groups before and after treatment.Result After 6 months of surgery,the therapeutic effect of the observation group was significantly higher than that of the control group(95.92％vs 79.59％)(P＜0.05).On postoperative days 3 and 7,the VAS score of the observation group was significantly lower than that of the control group,while the histopathological score was significantly higher than that of the control group(P＜0.05).After 6 months of surgery,the TAM score and sensory function grading in the observation group were(3.37±0.43)and(4.17±0.12)levels,respectively,higher than those in the control group(2.27±0.26)and(3.32±0.12)levels(P＜0.05).After 6 months of surgery,the psychological,physical,social,and material function scores of the observation group were significantly higher than those of the control group(P＜0.05).Conclusion The combination of microvascular technology and perforator flap repair has a significant therapeutic effect on patients with hand injury tissue injury,which can improve their hand sensation and motor function,alleviate pain,and improve tissue healing and quality of life.