Objective: Astragalus mongholicus could promote angiogenesis.Microemulsion has been widely used in the researches of traditional Chinese medicine in recent years.This study aimed to investigate the effect of Astragalus mongholicus microemulsion modified-collagen on angiogenesis of rats in vivo.Methods: Fifty SD rats were randomly divided into 5 groups: blank collagen,blank microemulsion modified-collagen,vascular endothelial cell growth factor(VEGF) modified-collagen,Astragalus mongholicus microemulsion modified-collagen and Astragalus mongholicus microemulsion plus VEGF modified-collagen.Then four collagen samples were imbedded in each rat.After the establishment of the models,the rats were executed at 3,7 and 14 days.The weights of the collagen samples were measured,and their hematoglobin and hydroxyproline levels were determined by the hemiglobincyanide colorimetric and sampler alkaline hydrolysis methods.The color of the wound surface was observed and the surrounding tissues were examined by HE staining for those killed at 14 days.Results: Both Astragalus mongholicus microemulsion and Astragalus mongholicus microemulsion plus VEGF significantly increased the production of hematoglobin and hydroxyproline(P0.05).Conclusion: Astragalus mongholicus microemulsion might promote angiogenesis by increasing the number of micrangia and the production of hematoglobin and hydroxyproline.

Objective To screen and confirm cell fusion by DNA technology of parentage identification based on detecting of short tandem repeats.Methods With 20％ polyethylene glycol (PEG)-6000,human myeloma cell lines and health individual peripheral blood mononuclear cell were fused.Then selected by hypoxantin,aminopterin,thymidin (HAT) medium,and fusion cell were sub-cloned.Morphology of fusion cells was checked by regular microscope.Concentration of DNA was compared to parental cells.Allele genes,identified by short tandem repeats,of fusion cell line were sequenced and compared with each other.Results The fused cells from myeloma cell line and peripheral blood mononuclear cell (PBMC) were slightly larger than primary cells,and the proliferation cycle was not changed significantly.DNA concentration of the fused cell DNA was increased by two times.Sequences of short tandem repeats (STR) showed that the fused cell included all original genetic materials of parent cells.Conclusions DNA technology of parentage identification is a convenient and reliable method to screen and confirm fused cell.

【Objective】 To investigate the feelings of blood donors during blood donation and its influencing factors, thus providing references for high quality blood donation service for blood banks. 【Methods】 A self-developed questionnaire concerning voluntary blood donation was randomly issued by the research group and recruited investigators among blood donors in 6 street blood donation sites of Nanjing Red Cross Blood Center at weekends during December 1~23, 2018. The basic information of blood donors, the difference of feelings during blood donation and the influencing factors were analyzed by descriptive analysis, chi-square test and binary Logistic regression analysis, respectively. 【Results】 A total of 405 questionnaires were issued, and all 405 copies were valid, with the response rate at 100% (405/405). Among the valid questionnaires, 46.67% (189/405) were first-time blood donors, 27.65% (112/405) had donated for 2~3 times, and 25.68% (104/405) donated more than 4 times. 53.59%(97/181), 23.7%(43/181) and 22.65%(41/181) of the above three groups of blood donors worried about the needles, and 55.48%(81/146), 22.60%(33/146) and 21.92%(32/146) worried about the blood outflow, 52.69% (88/167), 28.14% (47/167), and 19.16% (32/167 )worried about the pain during blood collection (P<0.05). The anxiety of unqualified blood test was similar to that of poor service (P>0.05). There were differences in blood donation experience among the three groups (P<0.05), and gender (P<0.05) was its influencing factor showed by binary Logistic regression analysis. 【Conclusion】 The majority of blood donors, who have donated at the street sites of Nanjing, are worried during blood donation collection. Therefore, the blood centers should provide high-quality services and humanized nursing interventions as well as strengthen the citizens′ awareness of blood donation.

Due to the high variation in test results of indirect enzyme-linked immuno sorbent assay (ELISA) and complicated steps involved in the process of standardization, a platform used for standardizing the test results from indirect ELISA was developed. The platform was designed based on 'Improved Standardization Method for Optical Density' (I-STOD). Gauss-Newton iteration was applied to estimate parameters in a standard formula. Programming Language VB was used for developing interface of platform. The results indicated that the validity of experiment could be verified through platform. A well determined scope of standardization could be generated. The sample with concentration within the scope was standardized and the degree of dilution was calculated for those outside the scope. The platform was successfully developed which normalized the process of standardization. The function provides the researchers with an effective and convenient tool for quickly achieving standardization of ELISA test results.
Antibodies ; analysis ; Enzyme-Linked Immunosorbent Assay ; methods ; standards ; Humans ; Optical Phenomena