AIM: To explore the correlativity of the oil/water partition of hydroxycamptothecin and its absorp-tion coefficiency in small intestine in rats.METHODS: pH value of water phase in buffer solution in turn took 2.2,4.6,7.4,9.6 and 11,while octanol was adopted as oil phase.After phase equilibrium,HPLC was used to determine the concentration of hydro-xycamptothecin,and oil/water partition coefficient was calculated with the phenol red as volume calibration agent,small intestinal absorption coefficients of rats in different pH were plotted against its oil/water partition coefficients.RESULTS: Oil/water partition coefficient(x) of hydroxycamptothecin and small intestinal absorption(y) altered with the change of pH value of the dispension medium.The linear re-gress equation was constructed as y = 0.001 3x + 0.009 1 with its correlation coefficient R2 being 0.959 7.CONCLUSION: There exists a good linear dependence between oil/water partition coefficient of hydroxycamptoth-ecin and its absorption coefficient in small intestines of rats.Oil/water partition coefficient can be used to design the new oral preparations.

Objective:To determine the relationship among tumor malignancy, invasion, and plasma D-dimer (DD) levels via a retrospective analysis of the correlation between preoperative plasma DD levels and postoperative pathology parameters in esophageal cancer patients. Methods:A total of 101 esophageal cancer patients without coagulation among preoperative plasma DD levels and postoperative pathology parameters in esophageal cancer patients who underwent radical operation between January 2009 to April 2010 were enrolled. The preoperative plasma DD levels, postoperative pathology T, N Grade, number of metastatic lymph nodes, tumor posi-tion, tumor length, and TNM stage were recorded. A statistic analysis of the above parameters was performed to reveal the correlation between plasma DD levels and tumor pathology features. Results:Preoperative plasma DD level has no correlation with Grade and tu-mor length, but shows positive correlation with pathology T, N status, and TNM stage, and has strong positive correlation with numbers of metastatic nodes. In addition, lower-third thoracic esophageal cancer patients have higher DD level than middle-third thoracic cases. Conclusion:Plasma DD level has a positive correlation with T stage, N stage, TNM stage, and number of positive lymph nodes. It also has important clinical significance.

Objective To understand the malignancy hospitalization expense of malignancy in Shanxi Cancer Hospital and provide a reference for the effective control of hospitalization expense.Methods 89 716 malignancy hospitalization cases in Shanxi Cancer Hospital from 2003 to 2010 were reviewed.Hospital costs of top six malignant tumors were analyzed.Results The hospitalization expense of 6 kinds of malignancy increased in varying degrees.In all cancer patients,the top six number of cases were lung cancer,cervical cancer,stomach cancer,breast cancer,esophageal cancer and colon cancer,there were a total of 89 716 passengers.The growth rate of cervix malignancy' s expense was the fastest.Conclusion Malignancy hospitalization is expensive,comprehensive measures need to be used to control the expense and make full use of health resources.

Objective: To study the efficacy and safety of the intrapleural injection of nedaplatin combined with recombinant hu-man endostatin (Endostar) in the treatment of malignant pleural effusion in non-small cell lung cancer (NSCLC) patients. Methods:Seventy patients diagnosed with NSCLC with malignant pleural effusion were treated by intrapleural injection. The subjects were divid-ed into two groups, as follows: 35 cases were injected with nedaplatin and Endostar (the treatment group) and 35 cases were injected with nedaplatin only (the control group). One month after the treatment, the clinical efficacy and toxicity were evaluated. The clinical ef-ficacy, quality of life, and toxic reaction were compared between the two groups. Results: The short-term clinical efficacy rate of the treatment group was 74.28%, whereas that of the control group was 48.57%, and the difference between the two groups was significant (P<0.05). Adverse effects such as nausea vomiting and leukopenia, occurred in both groups, but no significant differences were found between the two groups (P>0.05). Conclusion: The efficacy of the intrapleural injection of recombinant human endostatin combined with nedaplatin is superior to that of cisplatin alone in the treatment of malignant pleural effusion, without increasing chemotherapeutic toxicity.

AIM To observe the effects of the combination of heparinoid, aspirin and losartan on the experimental vascular intima hyperplasia induced by squeezing carotid artery in rats. METHODS Rats except the normal control group were under operation after two days oral administration once a day. At the second hour after injury, blood coagulation time(CT), time of arterial thrombus formation by electrical stimulus(ATFTES) and the level of TXB_2 in plasma were measured. At the fourteenth day after the surgery, the following indexes were examined: intima/media ratio, intima area ratio, PDGF-B and PCNA immunohistochemistry in carotid artery. RESULTS CT, TATFES increased and the level of TXB_2 decreased in the groups of Hep, Asp and Hep+Asp. The PDGF-B content, the positive ratio of PCNA and the inti- ma/media ratio also decreased in the groups of Hep, Asp, Los, Hep+Asp, Hep+Los and Asp+Los. Compared with single drug or two drugs combination, PDGF-B, positive ratio of PCNA, intima/media ratio and intima area ratio decreased significantly in the group of three drugs combination( P

BACKGROUND:Human periodontal ligament stem cels are a kind of mesenchymal stem cels that have self-renewal and multidifferentiation potential. Previous studies have showed that human periodontal ligament stem cels can differentiate into osteoblast-like cels or adipocyte-like cels under appropriate induction. Yet few studies have focused on the expression level of parathyroid hormone 1 receptor which wil affect the osteogenic potential of Human periodontal ligament stem cels. OBJECTIVE:To examine the expression level of parathyroid hormone 1 receptor between human periodontal ligament stem cels and human periodontal ligament cels and to discuss the role of parathyroid hormone 1 receptor in osteogenic differentiation. METHODS:By using magnetic-bead cel sorting, we separated and identified the human periodontal ligament stem cels and human periodontal ligament cels. We examined and compared the mRNA expression level of parathyroid hormone 1 receptor in human periodontal ligament stem cels and human periodontal ligament cels by Real-Time PCR. Osteoblastic differentiation was examined throughin vitro matrix mineralization by alizarin red staining and alkaline phosphatase assay. RESULTS AND CONCLUSION: Positive immunomagetic sorted cels were positive for STRO-1, CD146, Vimentin, indicating that they were periodontal ligament stem cels. Parathyroid hormone 1 receptor was expressed in human periodontal ligament stem cels and mainly located in cel membrane and cytoplasm which were similar to human periodontal ligament cels and MG63 cels. The expression of parathyroid hormone 1 receptor in human periodontal ligament stem cels was 3.7 times higher than that in human periodontal ligament cels, which was similar to that in MG63 cels. After osteogenic induction, human periodontal ligament stem cels showed a higher expression of parathyroid hormone 1 receptor and osteoblast-related genes as wel as the activity of osteoblast alkaline phosphatase and mineralization compared to human periodontal ligament cels. Our data showed that parathyroid hormone 1 receptor was higher in human periodontal ligament stem cels than human periodontal ligament cels and the expression was related with osteogenic differentiation, suggesting that human periodontal ligament stem cels display a higher potency of osteogenic differentiation and act as seed cels with a vast application prospect in oral tissue engineering.

Objective To investigate the influence of“double low”technology(low concentration iodinated contrast agent and low-dose scan) combined with body mass index(BMI) on radiation dose and image quality of contrast-enhanced upper abdominal CT examination. Methods One hundred and twenty patients who received upper abdominal enhanced CT examination were randomly divided into 4 groups:group A1, the iodinated contrast agent iodixanol(270 mg/ml), BMI<18.5 kg/m2 and 80 kVp;group A2, the iodinated contrast agent iodixanol(270 mg/ml), 18.5 kg/m2≤BMI≤24.9 kg/m2 and 100 kVp; group B1, the iodinated contrast agent ioversol(320 mg/ml),<18.5 kg/m2 and 120 kVp; group B2, the iodinated contrast agent ioversol(320 mg/ml), 18.5 kg/m2≤BMI≤24.9 kg/m2 and 120 kVp. Image quality was subjectively scored, the objective parameters(noise, CT values of abdominal aorta and liver parenchyma, contrast noise ratio of abdominal aorta and liver parenchyma) were evaluated and radiation dose was recorded. The differences of the indexes between A1 and B1 groups, A2 and B2 groups were compared with Mann-Whitney U test and pared-samples t test. Results All CT images were good. No images with 4 scores were obtained. No significant difference was found between group A1 and B1, between group A2 and B2(P>0.05). There was no significant difference in contrast noise ratio of liver parenchyma(P>0.05), while significant differences existed in CT values of abdominal aorta and liver parenchyma, contrast noise ratio of abdominal aorta between group A1 and B1(P<0.05). Significant differences existed in the parameters above mentioned between group A2 and B2, respectively(P<0.05). Radiation dose was lower in group A1 than in group B1 and in group A2 than in group B2(P<0.05), respectively. Radiation dose was decreased by 40.1%(0.89/2.22) in group A1 than group B1 while radiation dose decreased by 56.9%(3.02/5.31) in group A2 than group B2. Conclusion According to BMI, the low concentration iodinated contrast agent and low-dose scan CT scanning technology could effectively reduce radiation dose and generate ideal images during the contrast-enhanced upper abdominal CT examination.

ObjectiveTo evaluate the clinical effects of guiding apparatus assisted individual posterior cervical pedicle screw fixation technique.MethodsThe study enrolled 24 patients treated with posterior cervical decompressive single open-door laminoplasty and transpedicular screw-rod fixation from January 2008 to December 2010.The point of screw penetration and screw path direction were confirmed by measuring the transverse nail angle (TNA) and sagittal nail angle (SNA) of nail channel on the pre-operative CT plain scan of cervical pedicle and sagittal two-dimensional imaging of transpedicular axis.According to the results of CT measurement,individual cervical pedicle screw was implanted with the assistance of self-made guiding apparatus and then fixed after decompression.Transverse screw angle ( TSA ) and sagittal screw angle ( SSA ) were determined on the CT scan of cervical pedicle and sagittal two-dimensional imaging of transpedicular axis one week postoperatively in order to analyze the accuracy of placement of pedicle screws.Periodical anteroposterior and lateral X-ray radiographs of cervical vertebra were taken postoperatively to detect the stabilization of internal fixation.ResultsA total of 223 pedicle screws were inserted successfully into the C3 ～ C7,of which 220 crews were inserted accurately but three had slight inclination according to the postoperative CT,with placement accuracy of 98.7％.The comparison between inclination angle of inserted screws and that of preoperative transpedicular axis showed insignificant statistical difference ( P ＞ 0.05 ).All the patients were followed up for 6-34 months ( mean,18.5 months),which showed no neurovascular complications related to screws perforation out of pedicle cortex or no screw loosening,prolapse or breakage.ConclusionPosterior cervical pedicle screw insertionperformed according to the individual CT measurement is easy and safe and has a high accuracy rate under the assistance of self-made guiding apparatus.

Objective To summarize the features of multislice spiral computed tomography (MSCT) examination of gastrointestinal stromal tumors (GISTs),and investigate the relationship between predictors and risk of MSCT examination for GISTs.Methods The clinical data of 110 patients with GISTs who were admitted to the Tianjin Medical University Cancer Institute and Hospital from July 2011 to February 2014 were retrospectively analyzed.All the patients received 64-slices spiral CT (64S-SCT) or 16-slices spiral CT (16S-SCT) scan,and the data were transported to the PACS work station for multiplanar reconstruction.All the tumor samples were collected during operation and diagnosed by morphological manifestation and immunohistochemistry of tumors.Very low,low,and medium risk of GISTs were regarded as lower risk grade,and high risk of GISTs as high risk grade.The univariate analysis and multivariate analysis about features of imaging and risk were done by chi-square test and multivariate logistic regression model.Results Tumors located at the stomach in 81 cases,small intestines in 26 cases and colorectum in 3 cases.Diameter of tumors was 0.8-25.0 cm.Smaller tumors were in round or oval shape with well demarcated boundary,and larger tumors were irregular with unclear boundary.Endo-luminal growth of lessions was detected in 25 cases,duplex growth in 35 cases and extra-luminal growth in 50 cases.Enhanced CT scan showed that most of tumors in 105 patients demostrated moderate and high enhancement,heterogeneous enhancement in 74 cases,low density sacvariable necrosis area without enhancement in 60 cases and superficial,cracked-like and deep ulcer without calcification,metastasis and ascites in 23 cases.According to the features of GISTs by MSCT examination,location of tumor,diameter,shape,boundary,growth,enhancement,cystic necrosis,ulcer and metastasis were risk factors affecting risk classification of tumors by univariate analysis (x2=7.442,49.966,31.513,46.038,13.836,16.626,23.489,8.280,6.811,P ＜0.05).Diameter of tumor more than 10 cm and ulcer were independent risk factors affecting risk classification of tumors by multivariate analysis (OR =9.927,0.070 ; 95％ confidence intewal:1.888-52.180,0.012-0.398,P ＜ 0.05).Conclusion There is a characterization in the location,diameter,shape,boundary of tumor,growth,enhancement,cystic necrosis,ulcer and metastasis,and diameter of tumor more than 10cm and ulcer are independent risk factors affecting the risk classification of GISTs.

Objective To describe the quality of life of breast cancer patients and explore the risk factors for their quality of life (QOL). Methods QOL of 260 patients with breast cancer were studied using FACT-B, SCL-90, Social Support Rating Scale and multiple linear regression models to study the main factors influencing the QOL of patients with breast cancer on functional scales (physical, social, emotional, role). Results Economic conditions (β =2.024, P =0.003), emotion regulation ability (β =4.993, P =0.000) and self-evaluation of marital quality (β =-3.954, P =0.001) were associated with total QOL scores, emotion regulation ability (β =0.987, P =0.008), the number of emergency incidents (β=-0.866, P =0.011) were associated with physical domain scores, economic conditions (β =1.016, P =0.000), marital status (β =-1.018, P =0.041), emotion regulation ability (β =0.971, P =0.008), self-evaluation of marital quality (β =-1.382, P =0.001) were associated with social and family domain scores, emotion regulation ability (β =1.094, P =0.000), self-evaluation of marital quality (β =-0.892, P =0.006) were associated with emotional domain scores, regional factors(β =1.653, P =0.008), emotion regulation ability (β =0.962, P=0.01), self-evaluation of the QOL (β =-1.705, P =0.000) were associated with function domain scores. Conclusion Guiding the patients to have a correct understanding of the breast cancer, strengthening their confidence of life, increasing physical activity, having a good family environment and necessary social welfare so that improving the QOL of the patients.