【Objective】 To explore the diagnostic value of urinary liquid-based cytology (LBC) targeted fluorescence in situ hybridization (FISH) for urothelial carcinoma of the bladder. 【Methods】 Nuclear matrix protein 22 (NMP22) detection, urinary LBC and FISH were performed in 128 patients.The sensitivity and specificity of the three kinds of tests were analyzed with postoperative pathological results as the gold standard. 【Results】 The sensitivity of NMP22, urinary LBC and FISH was 61.11%, 79.17% and 82.46%, the specificity was 57.14%, 73.21% and 86.67%, respectively.The sensitivity of NMP22 and urinary LBC in detecting high BUC was better than that of low BUC, and the difference was statistically significant (P=0.01, P=0.03).There was no significant difference in the sensitivity of the three tests for the diagnosis of muscle-invasive bladder cancer and non muscle-invasive bladder cancer (P≥0.05). 【Conclusion】 Urinary LBC targeted FISH has high sensitivity and specificity in the diagnosis of urothelial carcinoma of the bladder and low-grade urothelial carcinoma.It can be an important method for the early screening and diagnosis of bladder cancer.