Objective To investigate the transfection of human papilloma virus types 16E6 gene on the proliferation and apoptosis of cervical carcinoma cell line C33A. MethodsPlasmid pcDNA3-HPV16E6 was stably transfected into C33A cells by liposome, and the expression of HPV16E6 mRNA and protein in the transfected cells, the cells transfected with blank vector and the cells without transfection were identified by RT-PCR and Western blot analysis. The growth, proliferation and cell cycles of the 3 kinds of C33A cells were by cell growth curve plotting and flow cytometry analysis. ResultsC33A cells were transfected stably by liposome with plasmid pcDNA3-HPV16E6 (C33A-E6 cells)and plasmid pcDNA3 (C33A-P cells). The growth of C33A-E6 cells was more rapid than those of C33A-P and C33A cells (P0.05). ConclusionHPV16E6 is involved in the cell cycle probably through some signal pathways, and promotes cell division and proliferation of C33A cells.

Purpose/Significance To probe into the characteristics of high-level talents in the medical field in recent years,and to provide references for formulating talent cultivation policies and effectively playing the role of scientific and technological talents in China.Method/Process Based on Elsevier's 2017-2021"Highly Cited Chinese Researchers"list,the distribution pattern and development trend of candidates in the medical field for 5 consecutive years are analyzed.Result/Conclusion The study shows that the candidates are mainly from universities and research institutes,which indicates that there is a positive relationship between scientific research achieve-ments and scientific research resource agglomeration.The number of people involved in clinical medicine and pharmacy has a significant advantage.In the past 5 years,most of the candidates have maintained high scientific research output and research activity.Based on this research,suggestions for the development of top talents in the medical field are proposed.