1.Some suggestions on clinical study of traditional Chinese medicine.
Chinese Journal of Integrated Traditional and Western Medicine 2006;26(5):455-458
According to the international accepted criteria, some suggestions were provided in this paper on the clinical study of traditional Chinese medicine. Firstly, allocate the participants randomly, rather than deliberately perform that to balance the baseline characteristics in groups. Secondly, use the international standard to evaluate the clinical effectiveness, rather than just use the simple criteria as cure rate, markedly effective rate, and effective rate. Thirdly, record and manage the missing data with proper statistical methods. Fourthly, objectively report the results of study, especially avoiding selective bias. In addition, the low limit effects should be avoided when assessing the effects of TCM in treating difficult and uncertain diseases; furthermore, the ethical problems should also be considered.
Clinical Medicine
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methods
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standards
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Diagnosis, Differential
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Humans
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Medicine, Chinese Traditional
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methods
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Randomized Controlled Trials as Topic
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Research Design
2.A pedigree with distal arthrogryposis.
Qian WANG ; Xuan LIU ; Fengjuan ZHOU ; Xiaorong ZHAO ; Wenmei XIE
Chinese Journal of Medical Genetics 2017;34(6):936-937
Adolescent
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Adult
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Aged, 80 and over
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Arthrogryposis
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genetics
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Child
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Female
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Humans
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Male
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Middle Aged
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Pedigree
3.Effects of fluoride on Fas signal pathway in rat incisor cells.
Ling-Fei HE ; Zhi-Hui ZOU ; Yuan-Fang ZHONG ; Qian XIE ; Xuan PAN ; Ri-An YU
Chinese Journal of Stomatology 2011;46(6):347-351
OBJECTIVETo investigate the effects of fluoride on Fas expression, caspase-3 and caspase-8 activity and apoptosis in rat incisor cells.
METHODSForty male SD rats were divided into 4 groups randomly and provided with distilled water containing NaF at the doses of 0, 10, 50 and 100 mg/L respectively. Each group had 10 animals. Five animals were sacrificed at 60 and 90 days respectively. Fas expression was measured with immunohistochemistry, and colorimetric assay was used to examine caspase-3 and caspase-8 activity with enzyme-labelled meter. The apoptosis was detected by flow cytometry in mandibular incisor cells.
RESULTSNaF at the doses of 10, 50 and 100 mg/L for 60 d and 90 d caused Fas overexpression, promoted activity of caspase-3 and caspase-8, increased apoptosis rate in mandibular incisor cells. At 60 days, the value of Fas expression was 0.1819 ± 0.0025 for control, 0.2120 ± 0.0084 for 10 mg/L NaF group, 0.2283 ± 0.0183 for 50 mg/L NaF group, 0.2818 ± 0.0233 for 100 mg/L NaF group. At 90 days, the value of Fas expression was 0.2077 ± 0.0289 for control, 0.2216 ± 0.0105 for 10 mg/L NaF group, 0.2377 ± 0.0059 for 50 mg/L NaF group, 0.2775 ± 0.0088 for 100 mg/L NaF group. Statistics analysis yielded close relationship between the dose of NaF in water and the Fas expression, and also between the dose of NaF in water and caspase-3 activities, and the relative coefficient was 0.9728 (60 d, P < 0.01) and 0.9889 (90 d, P < 0.01) for Fas expression, 0.9533 (60 d, P < 0.01) and 0.9849 (90 d, P < 0.01) for caspase-3 activity respectively. Apoptosis rate and caspase-8 activity also had close relationship with the NaF doses, and the relative coefficient was 0.9733 (90 d, P < 0.01) for apoptosis, 0.9928 (90 d, P < 0.01) for caspase-8. At the doses of 10, 50 and 100 mg/L NaF for 60 d and 90 d, obvious relationship was found between Fas expression and caspase-3 activity, and the relative coefficient was 0.9619 (60 d, P < 0.01) and 0.9912 (90 d, P < 0.01). Obvious relationship between Fas expression and apoptosis, between Fas expression and caspase-8 activity was found in groups for 90 d, and the relative coefficient was 0.9841 (P < 0.01) for apoptosis, 0.9767 (P < 0.01) for caspase-8.
CONCLUSIONSFluoride could induce Fas overexpression and mediate caspase activation and apoptosis at the doses of 10, 50 and 100 mg/L for 60 d and 90 d in rat mandibular incisor cells.
Animals ; Apoptosis ; drug effects ; Cariostatic Agents ; administration & dosage ; pharmacology ; Caspase 3 ; metabolism ; Caspase 8 ; metabolism ; Dose-Response Relationship, Drug ; Incisor ; cytology ; metabolism ; Male ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Signal Transduction ; Sodium Fluoride ; administration & dosage ; pharmacology ; fas Receptor ; metabolism
4.Tissue microarray and in situ hybridization study on hypoxia inducible factor 1alpha mRNA expression in epithelial ovarian tumor.
Jun LUO ; Zhi-lan PENG ; He WANG ; Yi-fan CHENG ; Kai-xuan YANG ; Lan XIE ; Xiao-lei QIAN ; Yan ZOU
Chinese Journal of Oncology 2003;25(2):168-170
OBJECTIVETo study the expression and significance of hypoxia inducible factor 1alpha (HIF-1alpha) in epithelial ovarian tumors.
METHODSThe expression of HIF-1alpha mRNA in 295 patients with epithelial ovarian tumor was analyzed retrospectively by high-throughput tissue microarray and in situ hybridization, which was compared with 13 normal ovarian tissue samples.
RESULTSThe expression rates of HIF-1alpha mRNA were 0, 13.2%, 42.1% and 81.9% in normal ovarian tissue, benign, borderline and malignant ovarian tumors. Expression rate of HIF-1alpha mRNA in borderline and invasive tumor was significantly higher than those in normal ovarian tissue and benign tumor (P < 0.001). Statistical analysis revealed that the expression of HIF-1alpha mRNA was not related to FIGO stages or histological subtypes. Close negative relation was observed between the expression of HIF-1alpha mRNA and tumor histological differentiation (P < 0.001).
CONCLUSIONThe overexpression of HIF-1alpha may play an important role in oncogenesis of epithelial ovarian tumor. Tissue microarray is an efficient technique of molecular biology.
Adult ; Aged ; Female ; Humans ; Hypoxia-Inducible Factor 1, alpha Subunit ; analysis ; genetics ; In Situ Hybridization ; methods ; Middle Aged ; Neoplasms, Glandular and Epithelial ; chemistry ; metabolism ; Ovarian Neoplasms ; chemistry ; metabolism ; Ovary ; metabolism ; RNA, Messenger ; analysis ; Tissue Array Analysis ; methods
5. Antibody persistence following on different vaccination strategies of domestic measles, mumps and rubella combined attenuated live vaccine: a 3-year follow-up study
Hanqing HE ; Qian LI ; Rui YAN ; Yang ZHOU ; Xuewen TANG ; Xuan DENG ; Shuyun XIE ; Zhiping CHEN
Chinese Journal of Preventive Medicine 2017;51(4):336-340
Objective:
To assess the 3-year antibody persistence after vaccination of domestic measles, mumps and rubella combined attenuated live vaccine (MMR) with different program.
Methods:
Children from three different vaccination strategies (Group 8 m MR: 8 months and 18 months vaccinated with measles-rubella combined attenuated live vaccine and domestic MMR,respectively; Group 8 m MMR: 8 months and 18 months both vaccinated with domestic MMR; Group 12 m MMR: 12 months and 22 months both vaccinated with domestic MMR ) were followed up in Zhejiang province in July 2015. There were 170 participants in Group 8 m MR, 171 participants in Group 8 m MMR and 173 participants in Group 12 m MMR selected by simple random sampling method .Blood samples (venous blood 2-3 ml) were collected 1 month after the first dose vaccination of MMR (only in Group 8 m MMR and Group 12 m MMR) and 3 years (36-38 months) after the last dose vaccination of MMR and tested for antibody IgG against Measles, Mumps and Rubella using ELISA. Seropostive rate and Geometric mean concentration (GMC) were calculated and compared among different groups by Chi-square test or Fisher exact test and Kruskal-Wallis
6.A Preliminary Study on the Construction and Visualization of Knowledge Graph for the Ancient Chinese Medical Book Ling Shu
Ying-Xuan CHEN ; Wei-Hao XIE ; Fan CHEN ; Qian XU ; Rong-Yao LI ; Zhen-Hu CHEN ; Xiu-Feng LIU
Journal of Guangzhou University of Traditional Chinese Medicine 2024;41(3):782-790
Objective To explore the construction and visualization for knowledge graph of Ling Shu(Spiritual Pivot),with a view to providing ideas for the structured storage and display of the theoretical knowledge of the ancient Chinese medical books.Methods Using the professional idea of constructing knowledge graphs for reference,text mining technology was applied to construct the thesaurus,and then word division,entity recognition,and relationship extraction for the original text of Ling Shu were performed to get the elements of knowledge graph construction.The graph database Neo4j was used for the storage and query of the knowledge graph,and then the visual display of the knowledge graph was achieved.Results The 1 216 high-quality words consisting of the thesaurus of Ling Shu were obtained,and the construction of the knowledge graph of the theory of Ling Shu was realized.The constructed knowledge graph basically displayed the traditional Chinese medicine theories such as the correlation of visceral manifestations with essence qi,and the relationship between emotions and the five-zang organs described in Ling Shu,which made the retrieval and utilization of the related entities and relationships possible,and provided ideas for the structured storage and display of the theoretical knowledge of the ancient books of Chinese medicine.Conclusion The knowledge graph construction technology can be used to obtain the Chinese medicine theoretical knowledge graph of Ling Shu,and to display the knowledge connections of yin-yang and the five elements,and the internal organs and meridians expressed in the Ling Shu.The construction of the knowledge graph and its storage in the graph database enable the knowledge graph involved in the text of Ling Shu to be displayed in the form of visualized semantic network graph,and also make the embedding of other search systems such as the semantic search and semantic wiki possible,which will be helpful for the development of Chinese medicine intelligent medical services.
7.The big data diagnosis-intervention packet payment method: experience from Shanghai and Guangzhou
Su XU ; Jinglei WU ; Hua XIE ; Li LIN ; Qian ZENG ; Xin CUI ; Jianwei XUAN ; Xiaohua YING ; Yujia YANG ; Yazhen YING
Chinese Journal of Hospital Administration 2021;37(3):186-190
Medical insurance payment model is transforming from project-based purchases to service bundle-based strategic purchases. The new form of bundled purchases should found on a scientifically-led design process of such bundles. The core to bundled purchase would be the payment standard, and the key to its success would be process control. Establishment of such a foundation, a core, and a key, would promote the current price standards, and lead service providers to a standardized medical service standard, so as to ensure a precise rewarding system of payment and service. The big data diagnosis-intervention packet(DIP)is able to fulfill mentioned ambitions by integrating insurance payment and supervision into one management. DIP is a full-process payment mode that encompasses pre-service estimation, in-service process control, post-service grading, and resource allocation. It is an innovative practice in line with China′s national conditions for the modern governance of medical security and medical services.
8.Mechanism of Fuzheng Qufeng Prescription in Suppressing Epithelial-mesenchymal Transition of Podocytes in Membranous Nephropathy Rats via TGF-β1/Smad Pathway
Xiao-yuan GUO ; Ming LEI ; Zi-wei SONG ; Jian WANG ; Xuan XIE ; Qian CAI ; Bao-kui WANG
Chinese Journal of Experimental Traditional Medical Formulae 2021;27(8):57-65
Objective:To study the effect of Fuzheng Qufeng prescription (FZQP) on transforming growth factor-
9.Efficacy and safety of decitabine combined with low-dose cytarabine, aclarubicin, and granulocyte colony-stimulating factor compared with standard therapy in acute myeloid leukemia patients with TP53 mutation.
Si-Si CHEN ; Qian SUN ; Lan CAO ; Wen-Zhong WU ; Yue XIE ; Chun QIAO ; Jian-Yong LI ; Si-Xuan QIAN ; Ming HONG
Chinese Medical Journal 2020;134(12):1477-1479
10.Meaning, Efficacy and Mechanism of Huoxin Pills in Treating Coronary Heart Disease with Qi Deficiency and Blood Stasis Syndrome
Xiu-juan YANG ; Rui-xuan ZHU ; Ran XIE ; Lei YANG ; Jin-cheng CHEN ; Bao-xin XU ; Xiao-chun SHI ; Peng-qian WANG ; Hai-ru HUO ; Yu-qing TAN ; Lyu GAO ; Feng SUI
Chinese Journal of Experimental Traditional Medical Formulae 2022;28(9):205-213
Cardiovascular diseases, with high incidence and high mortality, belong to the category of "chest impediment and heart pain" in traditional Chinese medicine (TCM). Chinese medicines have unique effect on the prevention and treatment of cardiovascular diseases with little side effects. Huoxin pills, one of the National Essential Drugs, is formulated based on the basic pathogenesis of weak pulse at Yang and wiry pulse at Yin and the pathological basis of myocardial ischemia and hypoxia and used for treating angina pectoris of coronary heart disease (Qi deficiency and blood stasis syndrome). This medicine is derived from the classic famous prescription and is composed of ten precious Chinese medicinal herbs. It can replenish Qi, activate blood, and warm collaterals to diffuse impediment by enhancing myocardial contractility and cardiac output to improve micro-circulation and increase coronary blood flow, regulating immune functions, alleviating inflammation, detoxifying, and tranquilizing mind. Clinically, it is suitable for patients with angina pectoris caused by the lack of heart Yang, chest tightness, shortness of breath, palpitation, fear of cold for limbs and so on, especially for the elderly with Yang deficiency or the patients with a history of myocardial infarction. On the basis of the available research reports, this paper explains the formula meaning of Huoxin pills from the perspective of the basic pathogenesis of coronary heart disease and predicts its action targets, location and links. Furthermore, we expound the mechanism of action of Huoxin pills based on basic research and clinical evidence-based research, aiming to provide data support and evidence for the clinical application of this medicine.