1.Expression of core components of Wnt2 signaling pathway in gliomas.
Guang-xiu WANG ; Zhi-yong ZHANG ; Pei-yu PU ; Chun-sheng KANG ; Shi-zhu YU ; Zhi-fan JIA ; Peng XU ; Xuan ZHOU
Chinese Journal of Pathology 2009;38(7):481-482
Astrocytoma
;
genetics
;
metabolism
;
Brain Neoplasms
;
genetics
;
metabolism
;
Frizzled Receptors
;
genetics
;
metabolism
;
Glioblastoma
;
genetics
;
metabolism
;
Glioma
;
genetics
;
metabolism
;
Humans
;
Paraffin Embedding
;
RNA, Messenger
;
metabolism
;
Receptors, G-Protein-Coupled
;
genetics
;
metabolism
;
Signal Transduction
;
Wnt2 Protein
;
genetics
;
metabolism
;
beta Catenin
;
genetics
;
metabolism
2.Preliminary study on aberrant expression of miRNAs related to the formation of the distinction between pediatric and adult types of brainstem gliomas.
Xuan WANG ; Hong-wei ZHANG ; An-ling ZHANG ; Lei HAN ; Kun WANG ; Pei-yu PU ; Chang-hong SHEN ; Chun-sheng KANG ; Chun-jiang YU
Chinese Journal of Surgery 2012;50(11):1015-1020
OBJECTIVESTo study the different expression of miRNA between pediatric and adult types of brainstem gliomas, and to provide the target miRNAs for explore the mechanism and miRNA interference of the malignant progression of pediatric BSG.
METHODSmiRNA expression profiles in orthotopic models which could simulate the BSG heterogeneity were examined by microarray and analyzed to obtain the aberrantly expressed miRNAs. The two types of human BSG tissue were utilized to verify the microarray data by qRT-PCR and in situ hybridization for the putative causative miRNAs.
RESULTSThere were 216 miRNAs detected in both the pediatric BSG group and the adult BSG group, 39 miRNAs to be differential expressed in the pediatric BSG group versus adult group, including 10 up-regulated and 29 down-regulated. qRT-PCR and in situ hybridization indicated good consistency with that of the microarray method.
CONCLUSIONSAberrantly expressed miRNA may serve as putative causative involvement of malignant progression of pediatric BSG, thereby might be potentially novel targets for therapy.
Adult ; Age Factors ; Animals ; Brain Stem ; Brain Stem Neoplasms ; metabolism ; Child ; Disease Models, Animal ; Female ; Gene Expression Profiling ; Glioma ; metabolism ; Humans ; In Situ Hybridization ; MicroRNAs ; metabolism ; Oligonucleotide Array Sequence Analysis ; Rats
3.Study on the anti-invasion effect of SEPT7 gene for U251MG glioma cell in vitro.
Song XU ; Zhi-fan JIA ; Qiang HUANG ; Chunsheng KANG ; Guang-xiu WANG ; An-ling ZHANG ; Xiao-zhi LIU ; Xuan ZHOU ; Peng XU ; Pei-yu PU
Chinese Journal of Medical Genetics 2008;25(3):262-267
OBJECTIVETo study the anti-invasion effect of SEPT7 gene on U251MG glioma cells and its possible molecular mechanism.
METHODSRecombinant adenovirus vector carrying SEPT7 gene (rAd5-SEPT7) was transduced to human glioma cell line U251MG, and empty adenovirus vector was used as control. Tumor invasion was examined by Transwell method and 3 D-Matrigel assay, and tumor cell migration by wound-healing method and 2 D-Matrigel assay. Three major molecular events associated with cell motility and migration, including changes of expression in MMP2, MMP9, MT1-MMP, TIMP1 and TIMP2, the alteration of integrin alpha(v)beta(3) expression, and the structural change of cytoskeleton protein, tubulin-alpha, in U251 cells transduced with rAd5-SEPT7 were studied by Western blotting, immunofluorescence and laser scanning confocal microscope, respectively.
RESULTSThe invasive and migratory capabilities of cells transduced with rAd5-SEPT7 were inhibited. The expression of extracellular matrix metalloproteinases MMP-2, MMP-9, MT1-MMP and integrin alpha(v)beta(3) was significantly decreased, while the expression of matrix metalloproteinase inhibitor TIMP1, TIMP2 was upregulated. Intracellular cytoskeleton protein-tubulin-alpha in U251 cells exhibited prominent morphological changes which including the appearance of distortion and aggregation resulting from redistribution of tubulin-alpha, and this feature of alteration was similar to the tubulin-alpha structure in normal non-tumor cells.
CONCLUSIONSEPT7 gene can inhibit the invasion and migration ability of U251 glioma cells. Its molecular mechanism may include that SEPT7 gene reverses the imbalanced state of MMPs/TIMPs, downregulates the expression of integrin alpha(v)beta(3) and alters the structure of tubulin-alpha of U251MG glioma cells. It is suggested that SEPT7 gene could be a good candidate for gene therapy of gliomas.
Adenoviridae ; genetics ; Blotting, Western ; Cell Cycle Proteins ; genetics ; physiology ; Cell Line, Tumor ; Cell Movement ; genetics ; Genetic Vectors ; genetics ; Glioma ; metabolism ; pathology ; Humans ; Integrin alphaVbeta3 ; metabolism ; Matrix Metalloproteinase 14 ; metabolism ; Matrix Metalloproteinase 2 ; metabolism ; Matrix Metalloproteinase 9 ; metabolism ; Microscopy, Confocal ; Neoplasm Invasiveness ; genetics ; Septins ; Tissue Inhibitor of Metalloproteinase-1 ; metabolism ; Tissue Inhibitor of Metalloproteinase-2 ; metabolism
4.Preliminary investigation of animal model of tibial defection fracture made by drilling hole method .
Wan-Qiang ZHANG ; Jian-Qing LIU ; Xuan WANG ; Hai-Xia TIAN ; Zhen WANG ; Qing-Hui SONG ; Jia ZHU ; Yuan MA ; Hong-Pu ZHAO
China Journal of Orthopaedics and Traumatology 2012;25(6):509-511
OBJECTIVETo compare the differences in damage degree, healing time and healing process between two animal models of defection fracture and provide the research data for the establishment of a new animal model.
METHODSSixty male SPF Spragur-Dawley rats were divided randomly into control group, sawing group and drilling hole group, with 20 rats in each group. Animals of drilling hole group were treated with hone drill and made an 1 mm hole on tibial plateau animals of sawing group were treated with saw and made an 3 mm defection on tibial plateau; nothing to do in animals of control group. The rats were respectively killed at 2nd, 4th weeks after operation, bone density, bone ash quantity, contents of bone Ca, P were detected.
RESULTSAt the 2nd week after operation, bone density, bone ash quantity, contents of bone Ca, P in drilling hole group and sawing group were significantly lower than that of the control group (P < 0.05 or P < 0.01). Two animal models of drilling hole group and sawing group were similar in X-ray films and pathology investigations. At the 4th week after operation, there were no significant defference in bone density and bone ash quantity among three groups (P > 0.05). Rats of sawing group showed more poroma and inflammatory infiltration in histological examination.
CONCLUSIONDrilling hole method and sawing method could be used to make animal model of defection fracture, two methods showed similar damage degree, healing time and healing process, hut drilling hole method have advantages of simple operation, easily control damage degree and less inflammatory infiltration and bone disunion.
Animals ; Bone Density ; Disease Models, Animal ; Male ; Rats ; Rats, Sprague-Dawley ; Tibia ; pathology ; Tibial Fractures ; etiology
5.Suppressive effect of knockdown of miR-21 expression on U87 human glionto growth in vivo
Xuan ZHOU ; Chun-Sheng KANG ; Pei-Yu PU ; Yong-Ping YOU ; Peng XU ; An-Ling ZHANG ; Guang-Xiu WANG ; Zhi-Fan JIA ; Xiao-Zhi LIU ; Song XU ; Zhen FU ; Peng ZHAO
Chinese Journal of Neuromedicine 2008;7(9):881-885
Objective To study the suppressive effect of knockdown of miR-21 on the U87 human giioma xenograft growth and the possible mechanism. Methods Nude mice bearing U87 human glioblastoma subcutaneously were treated with miRNA-21 anfisense oligonucleotides(AS-miR-21)intratumomlly every 3 d until the observation peded ended.The tumor volume of the mice treated withAS-miR-21 was measured regularly as compared with that in the control untreated mice and in the mice treated with scramble oligonucelotides(ODN).Finally,the tumors were removed from nude mice for the examination.In-sire hybridization and real-time PCR were conducted to detect the miRNA expression of miR-21.The biological charaetedsties of the tumors were evaluated by HE and immunohistochemieal staining, and the cell apoptosis was detected by TUNEL method. Resulls During the observation period,the tumor growth was delayed and the final tumor volume of AS-miR-21 heated group was smaller than that in the control and scramble ODN treatedg roup(F=6-056,P=0.007).The expression of miRNA precursor was knocked down in As-miRNA treated tunlors compared with that in untreated or scramble ODN treated tumors.Histopathological examination exhibited the appearance of degraded malignancy.The expressions of PCNA and MMP-9 were down-regulated while Septin-7 and P21 were up-regulated and apoptotic index was increased significantly (F=141.021,P=000) as well.Conclusion The suppressive effect of anti-miR-21 ODNs on the growth of U87 human glioma xenogratts is significant and miR-21 Call be taken as a candidate for gene therapy ofhuman glioma.
6.Cut-off points of fasting fingertip capillary blood glucose for detecting both undiagnosed diabetes and pre-diabetes
Yun-Liang ZHANG ; Shu-Qin GUO ; Wen-Bin MA ; Jun WANG ; Guang-Qin BAI ; Qian YANG ; Su-Fang TI ; Rui MA ; Rui-Pu WEI ; Wen-Xuan LIU ; Zhe LI ; Lei YANG ; Dian-Wu LIU ; Zhi-Hong LI
Chinese Journal of Epidemiology 2010;31(10):1174-1178
Objective To determine the efficient cut-off points of fasting fingertip blood glucose test for undiagnosed diabetes mellitus(DM), impaired glucose tolerance(IGT), and impaired fasting glucose(IFG)in community-based residents aged above 45 years old. Methods A cluster-randomized study was conducted from May 2008 to January 2009. A total of 3250 subjects aged above 45 years in two communities of Baoding city received questionnaire investigation and tested for fingertip blood glucose. Those subjects whose capillary blood glucose level ≥5.1 mmol/L were subjected to 75 g oral glucose tolerance test. Undiagnosed diabetes mellitus and pre- diabetes were identified by fasting plasma glucose and OGTT. In this study, the cut-off points of fasting capillary blood glucose for detecting undiagnosed diabetes and pre-diabetes were evaluated, using receiver operator characteristic curve(ROC). Results Of 1351 subjects that having had oral glucose tolerance test, 230 cases were diagnosed as diabetes mellitus(7.3%), 166 cases(5.2%)as IFG, and 204(6.7%)as IGT under fasting capillary blood glucose as test variable and state variables according to the following criteria.(1)FPG≥7.0 mmol/L or/and 2hPG≥11.1 mmol/L(2)FPG<5.6 mmol/L (3)FPG<7.0 mmol/L and 7.8 mmol/L≤2hPG≤ 11.1 mmol/L, areas under three ROC curves were 0.905, 0.633 and 0.719, respectively. The cut-offvalues of screening for undiagnosed DM, IGT and IFG were 6.0 mmol/L, 5.7 mmol/L, and 5.7 mmol/L, respectively. When cut-off value of screening for undiagnosed DM was 6.0 mmol/L, the maximal sensitivity was 78.0% and specificity was 89.3%.But there were both lower sensitivity and specificity in screening for IFG and IGT according to the best predicting value(5.7 mmol/L)from the ROC curves(50.3% and 28.0% vs. 60.8% and 28.0%). Conclusion Fasting capillary blood glucose with the lower cut-point of 6.0 mmol/L in screening for undiagnosed diabetes mellitus alone, was relatively reliable, whereas for both IFG and IGT the fasting fingertip blood glucose tests were fallible. It was convenient and could be used in screening the DM at the community level.
7.The mechanism of acute lung injury induced by nickel carbonyl in rats.
Ya Na BAI ; Li MA ; Qiu Ying WANG ; Hong Quan PU ; Xiao Pei ZHANG ; Xi Jiang WU ; Xiao Qiang XUAN ; Ning CHENG
Biomedical and Environmental Sciences 2013;26(7):625-628
Acute Lung Injury
;
chemically induced
;
metabolism
;
pathology
;
Animals
;
CDC2 Protein Kinase
;
genetics
;
metabolism
;
Cell Cycle
;
drug effects
;
Checkpoint Kinase 1
;
Female
;
Lung
;
metabolism
;
pathology
;
ultrastructure
;
Male
;
Malondialdehyde
;
metabolism
;
Microscopy, Electron, Transmission
;
Organometallic Compounds
;
toxicity
;
Oxidative Stress
;
Protein Kinases
;
genetics
;
metabolism
;
RNA, Messenger
;
metabolism
;
Rats
;
Rats, Sprague-Dawley
8.Expression of miR-140-5p and prediction of its target gene in human mesenchymal stem cells during adipogenic differentiation.
Tao WANG ; Rui-Qiao YAN ; Jun CAO ; Ling-Ling CAO ; Xuan-Pu ZHANG ; Xing-Nuan LI ; Ping WU ; Xiao-Ou ZHOU ; Jian-Fang WU ; Xiao-Yuan XU
Journal of Southern Medical University 2016;37(2):199-203
OBJECTIVETo screen the differentially expressed miRNAs and their target genes in adipogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs) to better understand the mechanism for regulating the balance between osteoblast and adipocyte differentiation.
METHODSCultured hMSCs were induced for adipogenic differentiation, and at 0, 7, 14, and 21 days of induction, the cells were examined for miRNA and mRNA expression profiles using miRNA chip and transcriptome sequencing (RNA-seq) techniques. Correlation analysis was carried out for the miRNAs and mRNAs of potential interest. The databases including TargetScan, PicTar and miRanda were used to predict the target genes of the differentially expressed miRNA.
RESULTSThe expression of miR-140-5p was down-regulated and leukemia inhibitory factor receptor (LIFR) expression increased progressively during adipogenic differentiation of hMSCs, showing a negative correlation between them. Target gene prediction using the 3 databases identified LIFR as the target gene of miR-140-5p.
CONCLUSIONmiRNA-140-5p may play an important role by regulating its target gene LIFR during adipogenic differentiation of hMSCs.
Adipocytes ; cytology ; Adipogenesis ; Cell Differentiation ; Cells, Cultured ; Down-Regulation ; Humans ; Leukemia Inhibitory Factor Receptor alpha Subunit ; metabolism ; Mesenchymal Stromal Cells ; cytology ; MicroRNAs ; genetics ; Oligonucleotide Array Sequence Analysis ; Osteoblasts ; cytology ; RNA, Messenger ; Transcriptome
9.Complications of hypertension in Minhang District of Shanghai based on electronic health records
Jiayun WANG ; Jun LI ; Tongtong LIANG ; Guo XUAN ; Pu LIU ; Jinling ZHANG ; Yuhong AO ; Jun LYU ; Chengyue LI
Shanghai Journal of Preventive Medicine 2022;34(9):895-900
ObjectiveTo determine the complications of hypertension among local residents in Minhang district of Shanghai, and provide scientific evidence for key preventive intervention. MethodsWe retrieved the data from the electronic health records, in which hypertensive patients were included for community-based management, in Minhang district of Shanghai from January 1st, 2014 to December 31st, 2018. A total of 38 599 hypertensive patients who had not had hypertension related clinical symptom when included in the electronic health records were enrolled in our study. Chi-square test and Cochran-Mantel-Haenszel test were used to determine the complications of hypertension. ResultsThe incidence proportion of complications was 10.77%, of which cerebrovascular damage was the highest (7.44%), followed by cardiac damage (3.55%) and peripheral vascular damage (0.81%). The incidence proportions in patients aged 18‒59, 60‒69, 70 and above were 5.53%, 9.61% and 16.19%, respectively. There was significant difference in the incidence proportions of complications among age groups (χ²=668.670, P<0.05). Cochran-Mantel-Haenszel test showed that in the hypertensive patients aged 60‒69, the incidence proportion of complications in females was lower than that in males (χ²=5.937, P<0.05). However, in those aged above 70, the incidence proportion of complications in females was higher than that in males (χ²=11.619, P<0.05). ConclusionIn patients with hypertension, the incidence proportions of cardiovascular and cerebrovascular diseases remains relatively high in Minhang district of Shanghai. Additionally, both age and gender have influence on the incidence of complications.
10.Two different techniques combined with MIS-TLIF in the treatment of degenerative lumbar spondylolisthesis:a case-control study.
Xing-Yu PU ; Wen-Yuan LUO ; Ming-Xuan GAO ; Gui-Fu MA ; Chao ZHANG ; Fei CHI ; Yao-Wen QIAN
China Journal of Orthopaedics and Traumatology 2022;35(5):409-417
OBJECTIVE:
To analyze the difference in clinical efficacy of minimally invasive transforaminal lumbar interbody fusion (MIS-TLIF) under Quadrant channel system combined with microscope and percutaneous pedicle screw in the treatment of degenerative lumbar spondylolisthesis.
METHODS:
A total of 114 patients with single-segment degenerative lumbar spondylolisthesis from June 2015 to February 2019, were divided into three groups according to the surgical methods, such as the MIS-TLIF under the microscope surgery group ( microscope group), MIS-TLIF combined with percutaneous pedicle screw technique surgery group(percutaneous group) and posterior lumbar interbody fusion surgery group (open group). In the microscope group, there were 12 males and 26 females, aged from 42 to 83 years with an average of (63.29±9.09) years. In the percutaneous group, there were 16 males and 22 females, aged from 45 to 82 years with an average of (63.37±7.50) years. In the open group, there were 12 males and 26 females, aged from 51 to 82 years with an average of (63.76±8.21) years. The general conditions of operation, such as operation time, intraoperative blood loss, postoperative drainage, length of surgical incision, frequency of intraoperative fluoroscopy and postoperative time of lying in bed were recorded to analyze the differences in surgical related indicators. Visual analogue scale (VAS) of waist and leg pain in preoperative and postoperative period (3 days, 3 months, 6 months and 12 months) were recorded to evaluate pain remission;Oswestry Disability Index(ODI), Japanese Orthopaedic Association (JOA) score were recorded to evaluate the recovery of waist and leg function on preoperative and postoperative 12 months. The lumbar spondylolisthesis rate and intervertebral height at 12 months after operation were recorded to evaluate the reduction of spondylolisthesis. The Siepe intervertebral fusion standard was used to analyze the intervertebral fusion rate at 12 months after operation.
RESULTS:
①All 114 patients were followed up more than 1 year, and no complications related to incision infection occurred. In the microscope group, there was 1 case of subcutaneous effusion 8 days after operation. After percutaneous puncture and drainage, waist compression, and then the healing was delayed. In the percutaneous group, 2 cases of paravertebral muscle necrosis occurred on the side of decompression, and the healing was delayed after debridement. In open group, there was 1 case of intraoperative dural tear, which was packed with free adipose tissue during the operation. There was no postoperative cerebrospinal fluid leakage and other related complications.① Compared with microscope group, percutaneous group increased in operation time, intraoperative blood loss, postoperative wound drainage, surgical incision length, intraoperative fluoroscopy times, and postoperative bed rest time. In open group, intraoperative blood loss, postoperative wound drainage, surgical incision length, and postoperative bed rest time increased, but the intraoperative fluoroscopy time decreased. Compared with percutaneous group, the intraoperative blood loss, wound drainage, surgical incision length, and postoperative bed rest time in open group increased, but operative time and the intraoperative fluoroscopy time decreased(P<0.05). ②ODI and JOA scores of the three groups at 12 months after operation were improved compared with those before operation (P<0.05), but there was no significant difference between the three group(P>0.05). ③Compared with microscope group, the VAS of low back pain in percutaneous group increased at 3 days after operation, and VAS of low back pain in open group increased at 3 days, and 12 month after operation. Compared with percutaneous group, the VAS low back pain score of the open group increased at 3 months after operation (P<0.05). ④ The lumbar spondylolisthesis rate of the three groups of patients at 12 months afrer operation was decreased compared with that before operation(P<0.05), and the intervertebral heigh was increased compared with that before operation(P<0.05), however, there was no significant difference among three groups at 12 months afrer operation(P>0.05). ⑤ There was no significant difference between three groups in the lumbar fusion rate at 12 months afrer operation(P>0.05).
CONCLUSION
The MIS-TLIF assisted by microscope and the MIS-TLIF combined with percutaneous pedicle screw are safe and effective to treat the degenerative lumbar spondylolisthesis with single-segment, and the MIS-TLIF assisted by microscope may be more invasive, cause less blood loss and achieve better clinical efficacy.
Blood Loss, Surgical
;
Case-Control Studies
;
Female
;
Humans
;
Low Back Pain
;
Lumbar Vertebrae/surgery*
;
Male
;
Minimally Invasive Surgical Procedures/methods*
;
Postoperative Hemorrhage
;
Retrospective Studies
;
Spinal Fusion/methods*
;
Spondylolisthesis/surgery*
;
Surgical Wound
;
Treatment Outcome