Objective To observe the effect of Guben Kechuan Gran-ules ( GKG ) on expression level of soluble interleukin -2 receptor (sIL-2R) of rats with chronic obstructive pulmonary disease (COPD). Methods Rat models with COPD made by fumigation and dripping li-popolysaccharide.The rtas were randomly divided into 6 groups:normal group, model group, high, middle, low doses ( 2.28, 1.14, 0.57 g? mL-1 ) GKG groups, and Guben Kechuan Tablets group.From the third day of modeling, medicines were administered to rats by means of intragastric administration for consecutive 28 days.Two days after the fin-ishing of intragastric administration,rats were executed and samples were collected.SIL-2 R expression level in rat was tested by means of ABC-ELISA method.Results SIL-2R expression level in low-dose groups of GKG decreased obviously,with a significant difference compared with model group ( P<0.05).Conclusion GKG can enhance immune func-tion and anti infection ability in the airway of rats with experimental COPD by regulating sIL-2R expression level.

Objective To observe the effect of Guben Kechuan Granules ( GKG) on expression level of T lymphocyte subsets in rats with chronic obstructive pulmonary disease ( COPD ) .Methods Rat models with COPD made by fumigation and dripping lipopolysaccharide . The rats were randomly divided into 6 groups:normal group, model group, high, middle,low doses (2.28,1.14,0.57 g? mL-1)GKG groups, and Guben Kechuan Tablets group .From the third day of modeling , medicines were administered to rats by means of intragastric administration for consecu-tive 28 days.Two days after the finishing of intragastric administration , rats were executed and samples were collected .T lymphocyte subsets ex-pression level in rat was tested by means of rapid immunohistochemistry method.Results T lymphocyte subsets expression level in low -dose groups of GKG decreased obviously , with a significant difference com-pared with model group ( P<0.05 ) .Conclusion GKG can enhance immune function in the airway in rats with experimental COPD by regula-ting T lymphocyte subsets expression level .

Objective To observe the effect of Guben Kechuan Gra-nules( GKG) on expression level of thymus index and spleen index of rats with chronic obstructive pulmonary disease ( COPD ).Methods Rats models with COPD made by fumigation and dripping lipopolysaccharide.The rats were randomly divided into 6 groups:normal group, model group, high,middle,low doses (2.28,1.14,0.57 g· mL-1) of GKG groups, and Guben Kechuan tablets group.From the third day of modeling, medicines were administered to rats by means of intragastric administration for consecutive 28 days.Two days after the finishing of in-tragastric administration,rats were executed and samples were collected. And then the spleen index and thymus index were calculated after the spleen and thymus were peeled from rats.Results Thymus index and spleen index expression level in GKG group increased obviously, with a significant difference compared with model group ( P <0.05 ) . Conclusion GKG can enhance immune function in the airway of rats with experimental COPD by regulating thymus index and spleen index expression level.

Objective To evaluate the role of imaging diagnosis in surgical treatment of primary aldosteronism(PA). MethodsFrom Jan 1995 to Dec 2004,245 patients with PA were hospitalized in our hospital.Before the operations,all the patients underwent B-ultrasonography and CT scaning,240 received intravenous pyelography and 75 MRI.The preoperative imaging diagnosis were compared with the findings during the operations and postoperative pathologic results. Results Compared with the findings during the operations,the accuracy rates of localized diagnosis for PA with B-ultrasonography,CT scanning and MRI were 92.7%,98.2% and 90.4%,respectively.Compared with the postoperative pathologic results,the accuracy rates of qualitative diagnosis for aldosterone-producing adenoma(APA) with B-ultrasonography,CT scanning and MRI were 83.0%,90.7% and 72.2%,respectively. Conclusion The comprehensive imaging data are helpful in the localized diagnosis of PA.Correct preoperative qualitative diagnosis of APA is the key step for the surgical treatment for PA.

Nematode sperm undergo a drastic physiological change during spermiogenesis (sperm activation). Unlike mammalian flagellated sperm, nematode sperm are amoeboid cells and their motility is driven by the dynamics of a cytoskeleton composed of major sperm protein (MSP) rather than actin found in other crawling cells. This review focuses on sperm from Caenorhabditis elegans and Ascaris suum to address the roles of external and internal factors that trigger sperm activation and power sperm motility. Nematode sperm can be activated in vitro by several factors, including Pronase and ionophores, and in vivo through the TRY-5 and SPE-8 pathways. Moreover, protease and protease inhibitors are crucial regulators of sperm maturation. MSP-based sperm motility involves a coupled process of protrusion and retraction, both of which have been reconstituted in vitro. Sperm motility is mediated by phosphorylation signals, as illustrated by identification of several key components (MPOP, MFPs and MPAK) in Ascaris and the characterization of GSP-3/4 in C. elegans.
Animals ; Helminth Proteins ; metabolism ; Male ; Nematoda ; cytology ; growth & development ; metabolism ; Phosphorylation ; Signal Transduction ; Sperm Motility ; Spermatozoa ; cytology ; growth & development ; metabolism

Objective To investigate the effects of Panax Notoginseng saponins (PNS) on protein expression of Klotho in rats with renal ischemia reperfusion injury; To discuss its protective mechanism for model rats. Methods Experimental rats were randomly divided into sham-operation group, model group, positive medicine group, PNS high-, medium- and low-dosage groups. Each administration group was given relevant medicine for gavage, once a day. Renal ischemia reperfusion injury model was established. Rats were sacrificed by taking blood from abdominal aorta after 4 hours of modeling. Serum levels of blood urea nitrogen (BUN), creatinine (SCr), malondialdehyde (MDA) content in kidney tissue, superoxide dismutase (SOD) activity and glutathione peroxidase (GSH-Px) activity were measured. HE staining was used to observe the morphological changes of renal tissue. The protein expressions of Klotho and NF-κB p65 were measured by immunohistochemical method. Results Compared with the sham-operation group, the levels of BUN and SCr in the model group increased significantly (P<0.05); protein expression of Klotho in renal tissue decreased and the protein expression of NF-κB p65 increased (P<0.05). Compared with the model group, the expression of Klotho increased but protein expression of NF-κB p65 decreased in each administration group (P<0.05); Compared with the positive medicine group, the expression of Klotho in PNS high-dosage group increased but protein expression of NF-κB p65 decreased (P<0.05). The protein expression of NF-κB p65 was negatively related to protein expression of Klotho (r=-0.895, P<0.05). Conclusion PNS can inhibit oxidative stress and anti-inflammatory effects through upregulating protein expression of Klotho, and reduce the protein expression of NF-κB p65, and thus exerts renal protective effects.

OBJECTIVETo investigate the therapeutic effect of free tissue flap anastomosed with reverse descendant branch of lateral femoral circumflex artery for severe soft tissue defect at leg.

METHODSThe severe soft tissue defect at leg, without any vessels for anastomosis of free tissue flap, was reconstructed with free tissue flap, which was anastomosed with proximal end of descendant branch of lateral femoral circumflex artery and great saphenous vein. From Oct. 2004 to Dec. 2009, 36 cases were treated with 15 cases of latissimus dorsi musculocutaneous flaps, 12 cases of anterolateral femoral flaps, and 9 cases of thoracoumbilicus flaps.

RESULTSAll the 36 free flaps survived completely. The patients were followed up for 6 months to 2.5 years with good cosmetic results.

CONCLUSIONSIt is effective and practical to repair the severe soft tissue defects at legs with the reverse descendant branch of lateral femoral circumflex artery to carry the free flaps.

Adult ; Female ; Femoral Artery ; surgery ; Follow-Up Studies ; Free Tissue Flaps ; Humans ; Leg Injuries ; surgery ; Male ; Middle Aged ; Skin Transplantation ; methods ; Soft Tissue Injuries ; surgery ; Thigh ; surgery ; Treatment Outcome ; Young Adult

OBJECTIVETo explore the relationship between heparanase mRNA expression and clinicopathological parameters in human gastric cancer.

METHODSRT-PCR was used to detect the expression of heparanase mRNA in 43 human gastric carcinomas and 10 adjacent normal gastric tissues.

RESULTSHeparanase mRNA was expressed in 29 of the 43 cases of gastric cancer with a positive rate of 67.4%, which was significantly higher than that in adjacent normal gastric tissues (P = 0.013). The expression level was higher in late-stage tumors (stage III and IV) than in early-stage tumors (stage I and II) (P = 0.001), in tumors with invasion to serosa than those without serosal invasion (P = 0.009), in tumors with lymph node metastasis than those without lymph node metastasis (P = 0.018), and in large-sized tumors than in small-sized ones (P = 0.009). The expression was not correlated with patients' age, sex, tumor location, histologic types, differentiation, peritoneal dissemination and liver metastasis (P > 0.05).

CONCLUSIONHeparanase might play an important role in the development of invasion and metastasis of gastric cancer.

Adenocarcinoma ; enzymology ; pathology ; Adult ; Aged ; Aged, 80 and over ; Female ; Glucuronidase ; biosynthesis ; genetics ; Humans ; Liver Neoplasms ; metabolism ; secondary ; Lymph Nodes ; pathology ; Lymphatic Metastasis ; Male ; Middle Aged ; Neoplasm Invasiveness ; Neoplasm Staging ; RNA, Messenger ; biosynthesis ; genetics ; Stomach Neoplasms ; enzymology ; pathology

Adolescent ; Adrenal Cortex Diseases ; complications ; pathology ; surgery ; Adult ; Child ; Female ; Humans ; Hydrocortisone ; blood ; urine ; Male ; Middle Aged ; Pigmentation Disorders ; complications ; pathology ; surgery

OBJECTIVETo evaluate and compare the results of vagina vasorum lymph node dissection (VLND) and non-vagina vasorum lymph node dissection (NVLND) in patients with gastric cancer after radical operation.

METHODSA total of 759 cases of evaluable patients with gastric cancer, operated from June 1994 to April 2005, were retrospectively analyzed. Of which, 627 cases underwent radical gastrectomy: 215 patients received VLND and 412 cases received NVLND. The operation time, intraoperative blood loss, operative complications and survival rate were recorded and compared between the two groups.

RESULTSThe 5- and 10-year overall accumulative survival rates of VLND group and NVLND group were 55.4% and 51.2%, 39.1%and 36.8%, respectively (all P < 0.05). No significant differences in intraoperative blood transfusion (loss), operation time, operative complication rate was found between the two groups. The 5- and 10-year accumulative survival in patients with a tumor of phase N0-N2, T2-T4, Ib-IV in VLND groups were all significant higher than those in NVLND group.

CONCLUSIONSVLND is a safe technique in advanced gastric cancer, it dose not prolong operation time or increase operative complications but improves survival.

Adult ; Aged ; Aged, 80 and over ; Female ; Follow-Up Studies ; Gastrectomy ; Humans ; Lymph Node Excision ; methods ; Lymphatic Metastasis ; Male ; Middle Aged ; Retrospective Studies ; Stomach Neoplasms ; pathology ; surgery ; Survival Analysis ; Treatment Outcome ; Young Adult