1.Percutaneous central venous catheter drainage for interventional treatment of complications in abdominal surgical diseases
Xin CHEN ; Zhidong XUAN ; Zhi ZHU
Chinese Journal of Primary Medicine and Pharmacy 2012;19(11):1652-1653
Objective To discuss the effica of central venous catheter drainage in interventional treatment of abdominal surgical complications.Methods The clinical data of 72 cases of abdominal surgical diseases were retrospectively analyzed,the complications included 23 cases of ascites,19 cases of bile leakage,8 cases of pancreatic fistula,7 cases of pancreatic necrosis and 15 cases of abdominal abscess,they all underwent percutaneous central venous catheter drainage under the guidance of ultrasound or CT.Results Central venous catheter was placed in 72 cases successfully in one time,the cathetem were kept for 2 to 37 days,5 discharged cases with catheter underwent regular reexamination in the clinic.3 cases of ascites were replaced for catheter shedding; 17 cases for occlusion recieved catheter irrigation and guidewire dredging;2 cases of pancreatic necrosis and 1 case of peripancreatic abscess were cured by repeated puncture and replacement of large-bore catheter.2 cases of pancreatic necrosis,1 case of pancreatic fistula and 1 case of appendicitis underwent laparotomy finally; 1 case of postcholecystectomy bile leakage underwent endoscopic stent therapy; 1 cases of severe pancreatitis complicated with ARDS was treated in ICU.Conclusion Central venous catheter drainage guided by ultrasound or CT for treatment of abdominal surgical complications was not only safe but also effective,and is worthy of popularization and application.
2.X-ray performance of melorheostosis in right foot:report of one case.
Qing-zhi XU ; Yan-qing HU ; Yu-xuan LIN ; Jiao-jiao HUANG ; Shu-mao YANG ; Zhu-yun CHEN
China Journal of Orthopaedics and Traumatology 2015;28(2):186-187
Adult
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Foot Diseases
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diagnostic imaging
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Humans
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Male
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Melorheostosis
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diagnostic imaging
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Radiography
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X-Rays
3.Expression of core components of Wnt2 signaling pathway in gliomas.
Guang-xiu WANG ; Zhi-yong ZHANG ; Pei-yu PU ; Chun-sheng KANG ; Shi-zhu YU ; Zhi-fan JIA ; Peng XU ; Xuan ZHOU
Chinese Journal of Pathology 2009;38(7):481-482
Astrocytoma
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genetics
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metabolism
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Brain Neoplasms
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genetics
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metabolism
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Frizzled Receptors
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genetics
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metabolism
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Glioblastoma
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genetics
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metabolism
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Glioma
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genetics
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metabolism
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Humans
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Paraffin Embedding
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RNA, Messenger
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metabolism
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Receptors, G-Protein-Coupled
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genetics
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metabolism
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Signal Transduction
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Wnt2 Protein
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genetics
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metabolism
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beta Catenin
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genetics
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metabolism
4.Purtscher-like retinopathy associated with antibiotic anaphylaxis.
Zhi-Xuan HUANG ; Zhi-Bing ZENG ; Zhu-Ping XU
Journal of Southern Medical University 2018;38(3):239-242
We report a case of an 18-year-old woman with systemic antibiotic anaphylaxis who presented anasarca and bilateral visual loss two weeks after the intravenous use of pazufloxacin. Ancillary fundus tests revealed bilateral cotton-wool spots, Purtscher flecken, edema, and retinal arteriolar occlusion around the optic disc. After pulse corticosteroid, administration of anti-anaphylactic agent, and general support therapy for one month, the patient showed a favorable change, with the symptoms lessened or free systemically, except the continuous aggravation of fundus ischemic change. After two intravitreal injections of Ranibizumab, there were still a large area of capillary non-perfusion and neovacularizations in the binocular retina, resulting in vitreous hemorrhage. The patient's visual acuity had still light perception after right-eye vitrectomy and presented no improvement during the postoperative follow-up of two years. Literature review revealed that there were many causes associated with Purtscher-like retinopathy, and the consequent visual impairment varied significantly. Prompt management of the underlying condition is crucial in giving the patient the best chance to restore vision.
5.Preparation of verapamil hydrochloride controlled-onset extended-release pellets and its pharmacokinetics in dogs.
Zhi-peng CHEN ; Yan-yu XIAO ; Hong-xuan CHEN ; Xi-jing CHEN ; La-rui LI ; Jia-bi ZHU
Acta Pharmaceutica Sinica 2006;41(8):765-771
AIMTo prepare verapamil hydrochloride controlled-onset extended-release pellets (VH-COERP) and study its release behavior in vitro. To compare the pharmacokinetic characteristics and bioavailability in six Beagle dogs after oral administration of VH-COERP and verapamil hydrochloride delayed-release pellets (VH-DRP) as reference.
METHODSThe core of VH-COERP were prepared in the fluidized bed (mini-glatt) by spraying water solution containing drugs onto sucrose-starch pellets with hydroroxy propyl methyl cellulose (HPMC) as the inner coating swelling layer and ethylcellulouse aqueous dispersion as the outer coating controlled layer. Through modifying the coating level of inner and outer layer, the VH-COERP with the optimized cumulative release profile was obtained. The concentration of VH in plasma of six dogs and its pharmacokinetic behaviors after oral administration of VH-COERP and VH-DRP at different times were studied by RP-HPLC. The pharmacokinetic parameters were computed by software program 3P97.
RESULTSThe lag time, the release behavior and the amount of VH from VH-COERP within 24 hours were not influenced by the pH of dissolution medium and post-process, but obviously influenced by the different kinds of added material in swelling layer and the coating level of the inner swelling layer and the outer controlled layer. In vitro the lag time of release profile of VH from VH-COERP was 5 h and then VH was extended release from VH-COERP in the following time. Compared with the VH-DRP, VH-COERP in vivo has an obviously lag time (4 h) , Tmax was also delayed (8 h) and the relative bioavailability was (94.56 +/- 7.64)%.
CONCLUSIONThe release profile of VH from VH-COERP was shown to be extended-release after an conspicuous lag time in vitro and in vivo. So the drug can be taken by the patient before bed time and begin to work at the morning.
Administration, Oral ; Animals ; Biological Availability ; Calcium Channel Blockers ; administration & dosage ; pharmacokinetics ; Cellulose ; analogs & derivatives ; chemistry ; Delayed-Action Preparations ; Dogs ; Drug Stability ; Hypromellose Derivatives ; Methylcellulose ; analogs & derivatives ; chemistry ; Microscopy, Electron, Scanning ; Verapamil ; administration & dosage ; chemistry ; pharmacokinetics
6.A novel "box lesion" minimally invasive totally thoracoscopic surgical ablation for atrial fibrillation.
Qiu Zhe GUO ; Da ZHU ; Zhi Xuan BAI ; Jun SHI ; Ying Kang SHI ; Ying Qiang GUO
Annals of the Academy of Medicine, Singapore 2015;44(1):6-12
INTRODUCTIONMinimally invasive surgical ablation is an emerging alternative method to catheter ablation and the full surgical maze procedure for nonpharmacologic treatment of atrial fibrillation (AF). We present a totally thoracoscopic "box lesion" radiofrequency ablation procedure in patients with paroxysmal or persistent AF.
MATERIALS AND METHODSFrom June 2011 to October 2012, 14 patients with lone paroxysmal (n = 7) or persistent AF (n = 7) were enrolled in this study. Procedures were performed through 3 5-12 mm holes on each side of the chest wall. A bipolar ablation device was used to create a box lesion in the posterior wall of the left atrium that encircled the 4 pulmonary veins (achieving bilateral pulmonary vein/posterior left atrial wall isolation). Perioperative complications were recorded for all patients. Freedom from AF was assessed by 24-hour Holter monitoring every 3 months or during symptoms of arrhythmia.
RESULTSThe ablation was successfully performed in all patients, with median operation time of 128 minutes (range, 45 to 180 minutes). No operative mortality or morbidity were noted during the study period. Freedom from AF was achieved in 12 patients (85.7%) during follow-up (median follow-up 9 months). One patient with persistent AF was shifted to paroxysmal AF. No atrial flutter or atrial tachycardia was noted during the follow-up.
CONCLUSIONThese early results show that totally thoracoscopic surgical ablation using a unique "box lesion" procedure for persistent or paroxysmal AF is a feasible and effective method with good short-term results. Further study is necessary to validate this result.
Adult ; Aged ; Atrial Fibrillation ; surgery ; Catheter Ablation ; methods ; Female ; Humans ; Male ; Middle Aged ; Thoracoscopy
7.Diagnostic and therapeutic methods for perioperative children with congenital heart disease with airway stenosis in pediatric intensive care unit.
Xuan XU ; Xi-cheng LIU ; Dan-dan LI ; Bin ZHU ; Li-jun XIAO ; Zhi-chun FENG ; Yi-min ZHU
Chinese Journal of Pediatrics 2013;51(11):842-845
OBJECTIVETo explore the diagnostic and therapeutic methods for perioperative children with congenital heart disease (CHD) with airway stenosis in pediatric intensive care unit (PICU).
METHODFiberoptic bronchoscopy was used for the diagnosis of 100 CHD cases in PICU who were clinically considered to have possible airway malformation because of complicated difficult-to-control lung infection, atelectasis and failure with the ventilator after surgery from January 2010 to October 2011. Cases who were confirmed to have severe airway stenosis by bronchoscopy and weaning from the ventilator after surgery were treated with balloon expandable stents into the desired position in the bronchoscopy.
RESULTThere were 73 cases (73%) of CHD patients with airway abnormalities, including 31 cases of severe stenosis (31%), moderate stenosis in 29 cases (29%), mild stenosis in 13 cases (13%). Nine of the 10 children in whom the mechanical ventilation was hard to be stopped after surgery because of severe airway stenosis were weaned from mechanical ventilation successfully by fiberoptic bronchoscopy, while one case died from primary disease with severe sepsis after the placement of bronchial stents.
CONCLUSIONCHD children with difficult-to-control lung infection, atelectasis and failure with ventilator after surgery are often complicated with airway abnormalities. The therapeutic bronchoscopy with airway stent can be used for cases with weaning from the ventilator because of severe airway stenosis.
Airway Obstruction ; diagnosis ; etiology ; therapy ; Bronchoscopy ; methods ; Constriction, Pathologic ; Female ; Follow-Up Studies ; Heart Defects, Congenital ; complications ; diagnosis ; surgery ; Humans ; Infant ; Infant, Newborn ; Intensive Care Units, Pediatric ; Lung Diseases ; diagnosis ; etiology ; therapy ; Male ; Perioperative Period ; Respiration, Artificial ; Stents ; Trachea ; abnormalities ; Tracheal Stenosis ; diagnosis ; etiology ; therapy ; Treatment Outcome
8.Identification of aging related proteins in human normal colonic epithelium.
Guo ZHU ; Zhi-qiang XIAO ; Zhu-chu CHEN ; Jian-ling LI ; Peng-fei ZHANG ; Yi-xuan YANG ; Xue-ping FENG ; Wei-jian YUAN
Journal of Central South University(Medical Sciences) 2005;30(6):625-630
OBJECTIVE:
To explore the molecular mechanisms of colonic epithelial aging related proteins and aged colonic epithelial susceptibility to tumor.
METHODS:
The proteins of normal human colonic epithelial tissue from young and old people were separated by 2-dimensional gel electrophoresis (2DGE), respectively. Then gels were stained by silver, scanned by imagescanner and analyzed with PDQuest software. The differentially expressed protein spots of colonic epithelium between the old and the young groups were identified by peptide mass fingerprint based on matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and database searching.
RESULTS:
Well-resolved and reproducible 2DGE maps of normal human colonic epithelium from the young and the old were acquired. Nineteen more than 2 fold differentially expressed protein spots were identified representing 17 different proteins by MALDI-TOF-MS. The functions of these proteins involve in metabolism, energy generation, transportation, antioxidation, translation and protein folding.
CONCLUSION
Seventeen aging related proteins of human colonic epithelium identified indicate that injury of mitochondrial function and decline of antioxidant capability are important reasons for the aging of human colonic epithelium. These data provided useful clues for elucidating the mechanisms of colonic epithelial aging and aged colonic epithelial susceptibility to cancer.
Aging
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metabolism
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Cells, Cultured
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Cellular Senescence
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genetics
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Chloride Channels
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biosynthesis
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genetics
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Colon
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cytology
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Electron-Transferring Flavoproteins
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biosynthesis
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genetics
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Epithelial Cells
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cytology
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Humans
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Intestinal Mucosa
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cytology
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Proteins
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metabolism
9.The effect of transmission electron microscopy on diagnosis of acute myeloid leukemia.
Shu-xu DONG ; Shi-xuan ZHAO ; Jin-hua LIU ; Ying-chang MI ; Xiao-fan ZHU ; Yu-mei CHEN ; Zhi-jian XIAO ; Yong-xin RU ; Jian-xiang WANG
Chinese Journal of Hematology 2013;34(3):205-207
OBJECTIVETo analyze coincidence rate of acute myeloid leukemia (AML) sub-typing between transmission electron microscopy (TEM) and clinical discharge diagnosis.
METHODSReviewing sub-typing results of TEM, light microscopy, flow cytometric analyzing, molecular biological detection and karyotype in 793 AML cases, comparing their coincidence rates with discharge diagnosis to reveal advantages of AML sub-typing by TEM.
RESULTSGeneral coincidence rates of TEM, light microscopy, flow cytometric analyzing, molecular biological detection and karyotype on AML sub-typing were 63%, 59%, 52%, 47%, 26% and 23% respectively, and clinical coincidence rates of TEM on M1, M2a, M4 and M5, M6, M7, t (8; 21) and t (15; 17) were 39%, 34%, 17%, 74%, 50%, 73%, 87% and 89% respectively.
CONCLUSIONTEM has a higher coincidence rate in general AML sub-typing, especially strong screenings on t (15; 17), t (8; 21), M7, M5 and M6, but lower coincidence rates on M1, M2a and M4 sub-typing than other methods.
Humans ; Leukemia, Myeloid, Acute ; classification ; diagnosis ; Microscopy, Electron, Transmission ; Retrospective Studies
10.Valuation on the immunization efficacy on the 23 years who had received plasma-derived HBV vaccine as newborns.
Huan-zhu ZHANG ; Wei-shou WU ; Fei SU ; Chao-mei SUN ; Ming-bo JIANG ; Guo-hua ZHANG ; Xuan-yi WANG ; Zhi-yi XU
Chinese Journal of Epidemiology 2012;33(2):207-209
OBJECTIVETo observe the long-term effect of plasma-derived HBV vaccine.
METHODSThe effect of a plasma-derived HBV vaccine which was given to children born in 1986 in Huangpu district in Shanghai were followed up once every two years and testing for HBsAg, anti-HBs and anti-HBc was carried out. Compared to background results from cross-sectional survey of hepatitis B virus in 1984 and 1985 (as internal control) as well as finding of survey targeted in non-plasma-derived HBV vaccine of children born in the same time in the nearby area from results investigated in 1991 (as external control), positive rate was calculated to assess the effect of protection.
RESULTSThe population immunized was followed up for 23 years and 5993 blood samples were collected. During the period of follow-up, the positive rate of anti-HBs decreased from 89.01% to 18.77% and the average level was 40.39%. The average positive rate of anti-HBc was 1.87%. The annual positive rate fluctuated around the average level. HBsAg positive rate remained less than 1.00% (0.46% - 0.98%), with an average of 0.62% (37/5993). Ranges of positive efficacy were from 81.37% to 95.78% against background control and 72.76% against external control.
CONCLUSIONThe plasma-derived HBV vaccine showed a good long-term protective effect and there was no need for boosting the immunization 23 years later.
China ; epidemiology ; Female ; Follow-Up Studies ; Hepatitis B ; epidemiology ; prevention & control ; Hepatitis B Antibodies ; blood ; Hepatitis B Vaccines ; immunology ; therapeutic use ; Humans ; Immunization Programs ; Infant, Newborn ; Male ; Vaccination