Objective To investigate the role of TRAF2 in AP-1 signaling pathway of human B cells.Methods Human Ramos B cells were transfected with plasmids expressing YFP fusion wild type TRAF2(YFP-WT-TRAF2) or YFP fusion dominant-negative TRAF2(DN-TRAF2),or transfected with shRNA-TRAF2 and control shRNA plasmid.After incubation overnight,cells were either sorted with flowcytometry or screened by antibiotics G418.Activation of AP-1 pathway,including phosphorylation of ERK,JNK and P38,as well as nuclear translocation of AP-1 subunits were detected by western blot and ELISA.Results Overexpression of WT-TRAF2 selectively induced activition of MAPK by phosphorylation of ERK and P38,and further induced nuclear translocation of C-FOS.Moreover,both overexpression of DN-TRAF2 and transfection of shRNA-TRAF2 inhibited phosphorylation of ERK and P38,and nuclear translocation of C-FOS.Conclusion TRAF2 selectively activates some kinases in CD40 mediated AP-1 signaling pathway,and plays an important role in AP-1 activation.

Objective To investigate the clinical characteristics of gliomas in children,and assess the value of CT and MRI for diagnosing glioma.Methods Fifty-three children under 14 years old who were admitted into department of neurosurgery of Henan Provincial People's Hospital from Jan.2001 to Dec.2006 were diagnosed as glioma.Their age and sex distribution,clinical symptoms and signs,imaging examinations and pathological examinations were retrospectively analyzed.Results 1.There were 35 males and 18 females.2.The common symptoms and signs were headache and dizziness(32 cases,60.4%),nausea and vomiting(32 cases,60.4%),balance function disorder(31cases,58.5%),episodic convulsion(6 cases,11.3%) and fundus changes(33 cases,62.3%).3.There were 24 gliomas located at supratentorial and 29 gliomas located at infertentorial in this group.4.Thirty-three cases had a CT examination(1 case was wrongly diagnosed) and 28 cases had a MRI examination(all cases were positive);The positive rate of CT for displaying these tumors was 97%,MRI was 100%.5.Pathological examination revealed gliomas in 42 cases after operation.The most common histologically confirmed tumor type reported was astrocytoma,which accounted for 40.5%(17 cases),and the second was medulloblastoma,which accounted for 31.0%(13 cases).Conclusions 1.Glioma are more frequently affected in males than females in children.2.It often occurs in cranial posterior fossa and midline,which causes the symptoms of intracranial hypertension including headache,dizziness,nausea,vomiting,fundus changes,and so on.3.It often occurs in infertentorial,which causes the symptoms of balance function disorder,since it can invade cerebellum directly or indirectly.4.CT and MRI play an important role in the diagnosis of glioma in children.MRI is more valuable than CT in glioma diagnosis.5.Histological types of glioma in children are significantly different from adults.The most common histological types are astrocytoma and medulloblastoma.

AlM:To observe clinical effects between the laser in situ keratomileusis ( LASlK ) surgery and the laser subepithelial keratomileusis ( LASEK ) surgery as the second operation after an unsuccessful LASlK surgery.METHODS:Forty-nine patients (98 eyes) with refractive regression after LASlK operation received the second surgery. All patients were divided into two groups:group A and B. Group A (48 eyes of 24 patients) received LASlK surgery and group B ( 50 eyes of 25 patients ) received LASEK surgery. lnspect the main parameters included visual acuity, refraction, corneal curvature, and the total value of high-aberration after 1wk, 1mo and 1a, t-test of groups was used as statistical analysis method.RESULTS: There was statistically significant (P<0. 05) between the two groups in visual acuity after 1wk. There was no statistically significant between the two groups in visual acuity after 1mo, and there was also no statistically significant between the two groups in visual acuity, average spherical equivalent refractive degree, average corneal curvature, and the total value of high-aberration after one year (P>0. 05). There developed one case of epithelium in growth after LASlK surgery, and one case of haze Ⅱ after LASEK surgery.CONCLUSlON: Both LASlK and LASEK surgery can obtain satisfactory therapeutic results after an unsuccessful LASlK surgery.

Objective To analyze the relationship between carotid intima-media thickness (CIMT) and metabolic syn?drome (MS), and explore risk factors for CIMT incrassation in Tianjin policemen. Methods Fifty MS patients (MS group) and 50 healthy people (control group) in health examination of Tianjin policemen were enrolled in this study. The question?naire survey(family history,past medical history,present history,living habits and occupational stress factors) were recorded in two groups of patients. The blood indicators (fasting plasma glucose, triglycerides, total cholesterol, high density lipopro?tein cholesterol, low density lipoprotein cholesterol, homocysteine) were detected. The color Doppler was used to measure CIMT incrassation, and incrassation ratio was calculated. The relative indicators were compared between two groups. The lo?gistic regression model was used to analyse the influencing factors of carotid artery intima-media thickness. Results CIMT and CIMT incrassation ratio (communis, internal and external carotid) were significantly higher in MS group than those of control group (P<0.01). The risk factors for CIMT included family history of hypertension, chronic stress, smoking, family history of diabetes, high fat diet and high homocysteine levels, and dairy products were protective factors for CIMT incrassa?tion. Conclusion The reason of carotid artery intima-media thickness is diversity. The healthy lifestyle and clinical inter?vention to retard CIMT are advocate, which are conducive to the control of atherosclerosis.

Objective To optimize the preparation process of Xiaochuan Tiegao. Methods The effective compound imporatorin of Dahurian Angelica Root was determined by HPLC. The best condition of preparation process for the ethanol-extraction method was investigated by L9(34) orthogonal design. Results The optimum technical condition:adding 2 times ethanol, cooking 1 hour and 2 times with 90% ethanol. Conclusion The method is reliable, accurate and easy to operate.

Objective To develop an HPLC method for the assay of sinapine thiocyanate in Semen Sinapis Albae of different processed products. Methods It was assayed by RP-HPLC. The column was Hypersil BDS Phenyl (5 ?m, 250 mm?4.6 mm), the mobile phase was acetonitrile-0.08 mol/L KH2PO4 (15∶85). The UV detection wavelength was 240 nm. The flow rate was 1.0 mL/min. Results Good linearity of sinapine thiocyanate was showed within the range of 0.8～4.0 ?g (r=0.999 7). The average recovery was 100.98% (RSD=3.51%). Conclusion The method is simple, accurate and good reproducible. It can be used for quality control of Semen Sinapis Albae of different processed products.

The evaluation of visual quality about human eyes has always been an important issue in the field of eye science and visual optics,and it is mainly divided into subjective evaluation method and objective evaluation method,evaluation indicators including visual acuity,contrast sensitivity,wavefront aberration and retinal straylight,and so on.In addition,according to different principles and standards,visual system imaging quality evaluation methods can also be divided into visual refractive system and retinal nerve system evaluation,geometrical optics and physical optics evaluation,static and dynamic visual function evaluation.The selection of visual quality evaluation method should be considered from multiple dimensions on comprehensive consideration.in recent years,the improvement of quality of life promotes the development of visual quality assessment methods,and more attention is paid to the evaluation of continuous functional visual acuity.This article reviews the research progress of visual quality assessment methods.

Objective To select the optimum bases for Sihuang Gao and study the method for quality control.Method Ultra-fine powders of Radix Scutellariae,Cortex Phellodendri,Radix et Rhizoma Rhei and Rhizoma Coptidis were used to prepare Sihuang Gao with different formulations,the bacteriostatic action of which were compared and stability were inspected.The best prescription was identified by TLC,and the skin irritation was observed.Result Sihuang Gao made of water-soluble base had a strong antibacterial activity and stable quality,and it was proved no irritation,the contents of active component could be detected by TLC.Conclusion The quality was controllable,and the improvement prescription can be used in the clinic.

Objective To evaluate the effects of percutaneous long sheath aspiration embolectomy for the treament of acute superior mesenteric artery embolism. Methods 7 cases (atrial fibrillation 4 cases; left atrium myxoma 2 cases and chronic mesenteric ischemia 1 cases) of acute measenteric artery embolism were treated with percutaneous emlolectomy using long sheath (Optimed, Germany) aspiration. Results Successful recanalizations were observed in all of the 7 cases of superior mesenteric arteries. 5 cases out of the 7 cases recovery; 1 case underwent laparotomy; 1 case died in 24 hours. Conclusions Percutaneous embolectomy using long sheath aspiration is a sample and effective method for the treatment of acute superior mesenteric artery embolism.

0.05),but costs spent by thymosin and lamivudine combination group was much less(P