OBJECTIVE: To analyse oily constituents of Meconopsis punicea Maxim. METHOD: The oily constituents were analysed and identified by GC-MS. RESULTS: The sixty-three constituents were isolated and the forty-seven of that were identified, making up 74.6% of the total constituents. Ester compounds accounted for 57.5% of the identified compounds. The main constituents were ester octadecnate. CONCLUSION: Ester octadecenate has high biological activity, which agrees with curative effect of the plant.

From 2004 to 2013,Peking Union Medical College had implemented technology innovation plans to build 12 innovation teams funded by Ministry of Education.This paper made statistical analysis of the results and achievements of the team building initiatives and put forward new ideas and measures for future development.

The article summarized and analyzed the research fund for doctoral programs at Peking Union Medical College during the 10th and 11th Five-Year period.A further exploration into the fund's management pattern was made and suggestions on how to improve the management were also proposed.

Objective: To analyze the main chemical constituents of traditional Chinese medicine compound Chenxiang Huaqi Pills by using UPLC-Q-TOF/MS technology. Methods: The separation was performed on Phenomenex Kinetex C18 column (100 mm×4.6 mm, 2.7 μm), and the gradient elution of acetonitrile-0.1% formic acid was used as mobile phase at a flow rate of 0.8 mL/min. The data was collected by the positive and negative ion modes using Q-TOF/MS and ESI source. The main chemical constituents of Chenxiang Huaqi Pills were identified according to the exact molecular mass, the cleavage fragments of MS/MS, the literature data, and the reference control. Results: A total of 73 chemical components were separated and identified in Chenxiang Huaqi Pills, including 36 flavonoids, 16 2-(2-phenylethyl) chromones, 7 triterpenoid saponins, 2 sesquiterpene lactones, and 12 other components. Conclusion: This study showed that UPLC-Q-TOF/MS technology provided a simple, rapid, and accurate method for the identification of chemical constituents in Chenxiang Huaqi Pills, which provided a new technology method for the pharmacological basis and quality control of Chenxiang Huaqi Pills.

The Union Youth Science & Research Fund was set up by the Peking Union Medical College (PUMC) using the Fundamental Research Funds for the central universities.Education Ministry's National College Special Fund for Basic Science& and Research drafted and implemented its own management rules.The rules standardize the fund's various aspects including its system construction,project classification,implementation terms,outlay management,and project management.In consequence,the rules promoted the young teachers' research on funded-subjects and improved the management level on scientific research projects.

Objective To study the relationship between endothel in-1 in plasma and TCD in patients with brain injury.Methods We measured dynamically the level of ET-1 in plasma on 33 adult brain injured patients. Cerebral vasospasm was detected by transcranal Doppler sonography (TCD).Results 1.During 6-8 days, 13-15 days after injury,there was a significantly positive correlation between the level of ET-1 in plasma and cerebral vasospasm (r=0.533,P=0.002;r=0.423,P=0.02),especially during 6-8 days after injury. The increase of the level of ET-1 in plasma paralleled paroled with the time of cerebral vasospasm developed. 2.During 6-8 days after injury, the level of ET-1 in plasma of patients with cerebral vasospasm was obviously higher than those without cerebral vasospasm obviously (P

Air Pollutants, Occupational ; analysis ; Chlorofluorocarbons, Methane ; analysis ; Chromatography, Gas ; methods ; Workplace

Objective To evaluate the value of plasma soluble urokinase plasminogen activator receptor (suPAR) and serum pmcalcitonin (PCT) to investigate their assessment of disease severity and prognosis in patients with sepsis.Methods The levels of plasma suPAR and serum PCT were monitored in 77 patients with sepsis.The acute physiology and chronic health evaluation Ⅱ (APACHE Ⅱ) and sequential organ failure assessment (SOFA) score were recorded.According to the disease severity and their prognosis,the value of plasma suPAR,serum PCT,APACHE Ⅱ and SOFA score on predict the disease severity and prognosis of septic patients were compared.Results The levels of plasma suPAR in septic patients [(7.9 ±6.5) ng/mL] were lower than severe sepsis patients [(8.4 ±4.5) ng/mL] and septic shock patients [(13.9 ± 8.0) ng/mL],allP ＜ 0.05.The levels of serum PCT in septic patients (6.3 ± 3.5) ng/mLwere lower than severe sepsis patients [(23.7 ± 3.9) ng/mL] and septic shock patients [(25.7 ±4.3) ng/mL],allP ＜0.05.But there was no significant difference in the levels of serum PCT between the severe sepsis group and the septic shock group.Receiver operator characteristic curve (ROC)of the level of plasma suPAR could distinguish survivors from non-survivors in septic patients,maximal area under curve (AUC) of plasma suPAR was 0.803.The best cut-off value of plasma suPAR to distinguish survivors from non-survivors was 9.905 ng/mL.And the AUC of serum PCT was 0.61 (P ＞ 0.05) ; the AUCofAPACHEⅡ score was 0.832 (P＜0.05); the AUC of SOFA score was 0.767 (P＜0.05).Conclusion Monitoring of the levels of plasma suPAR and the APACHE Ⅱ score can help to assess the severity and the prognosis of sepsis in the early stage.

Objective To assess the characteristics of different doses of cisplatin-induced acute kidney injury,further to understand mitochondrial dysfunction and its role in acute kidney injury (AKI).Methods Male C57BL/6J mice were first randomly divided into two groups:control group (n =6) and AKI group (n =12).Then,AKI group was subsequently divided into other two groups according to different dose of cisplatin (10 mg/kg or 20 mg/kg).AKI group received intraperitoneal injection of cisplatin.All mice were sacrificed after 72 h of injection.Renal biochemical function,renal pathological changes,renal injury markers,kidney mitochondrial function and structural changes were observed.Results (1) After 72 hours of injection,the AKI group performed significant kidney injury changes compared to control group,thereinto 20 mg/kg group was more serious than 10 mg/kg group.With the cisplatin dose increasing,renal function markers such as serum creatinine,urine protein gradually increased.(2)Kidney biopsy showed tubular structural damage,the formation of protein casts,kidney injury molecule-1 (KIM-1) gradually increased(P ＜ 0.05).(3)Electron microscopy found tubular mitochondrial structural damage,mtDNA copy number decreased,the level of peroxisome proliferatoractivated receptor-gamma coactivator-1alpha (PGC-1α),ATP synthase β decreased(P ＜ 0.05),and Western blotting manifested cytochrome C was released from mitochondria to the cytoplasm.These data all exhibited significant difference between different groups(P ＜ 0.05).Conclusions Cisplatin induces acute kidney injury in dose-dependent manner.Mitochondrial dysfunction participates in kidney injury,and is also related to the kidney pathological damage.

OBJECTIVETo investigate the effects of oral cancer-associated fibroblasts (CAFs) on lymphangiogenesis in oral squamous cell carcinoma (OSCC).

METHODSCAFs and normal fibroblasts (NFs) were obtained from the tissues of patients with OSCC who did not receive radio-chemotherapy before operation. And the CAFs and NFs were isolated by method of tissue block and identified by immunohistochemical staining. The effects of CAFs (group A) and NFs (group B) to human lymphatic endothelial cells (HLEC) were detected by using a 24-multiwell transwell cell culture chamber. DMEM sugar medium was as blank control group. The number of proliferative, migratory, invasive and tubes of HLEC were counted under inverted phase contrast microscope.

RESULTSThe proliferative number of HLEC of group A for 96, 144, 196 h was significantly higher than that of group B and blank control group, group B higher than blank control group (P<0.01). The migratory and invasive number of HLEC of group A for 96 h was significantly higher than that of group B and blank control group, group B higher than blank control group (P<0.01). The number of tube formation of HLEC of group A for 24 h was significantly higher than that of group B and blank control group, group B higher than blank control group (P<0.01).

CONCLUSIONCAFs promote HLEC's proliferation, migration, invasion, tube formation, and these effects are stronger than NFs.

Carcinoma, Squamous Cell ; Cell Culture Techniques ; Cell Movement ; Cell Proliferation ; Endothelial Cells ; Fibroblasts ; physiology ; Humans ; Mouth Neoplasms ; pathology