In daily medical work, it is common to carry out bedside ultrasound guided puncture surgery, which is easy to miss and inconvenient to operate when transporting medical devices. The department of emergency of the First Hospital of Jiaxing designed a kind of mobile ultrasound guided puncture vehicle, and obtained the National Utility Model Patent of China (ZL 2020 2 0100473.X). The utility model includes a car body, a work table, an ultrasonic machine, a storage cell and a material basket. The side of the car body is provided with an opening, and both sides of the opening surface are provided with a turnover mechanism, and the rotation axis of the turnover plate is horizontally arranged. The utility model can facilitate the operation of puncture bags, disinfection articles, monitors, oxygen bottles and other medical equipment, and can expand the use area of the operating platform, increase the storage function and store more. This device has good practical value.

Posterior cortical atrophy (PCA) is a kind of neurodegenerative dementia,which is characterized by the progressive decline of visuoperceptual,visuospatial,reading and writing ability and praxic skills.Neuroimaging usually shows atrophy or metabolic decrease in posterior brain regions.The most common neuropathologic changes of PCA are amyloid plaques deposition and neurofibrillary tangles in posterior cortex.In general,PCA is considered as a variant form of Alzheimer's disease.

Objective To explore the clinical effect of intravenous injection of 50％ glucose combined with furosemide in the treatment of heart failure.Methods 71 patients with intractable heart failure were selected.According to the random number table,they were divided into the observation group (35 cases) and control group (36 cases).On the basis of conventional treatment,the observation group received continuous intravenous infusion of furosemide 100mg combined with intravenous injection of 50％ glucose 100ml.The control group was given intermittent injection of furosemide 300mg,3 times/day,100mg/each time.Treatment effects were compared between the two groups.The cardiac function,24h urine volume,BNP and ultrasound beckoning figure index including left ventricular end-diastolic diameter (LVEDD),ejection fraction (EF) before and after 72h treatment of the two groups were compared.Results The total effective rate of the observation group was 82.86％,which was significantly higher than 61.11％ of the control group (x2 =4.15,P ＜ 0.05).After treatment,cardiac functional grading,24 hour urine volume,BNP,EF and LVEDD of two groups were significantly improved,and compared with the control group,the improvement of the observation group was more significant,the differences were statistically significant (t =3.23,2.98,3.87,3.12,3.67,P ＜ 0.05).The incidence rate of adverse reaction of observation group was 8.57％,that in control group was 13.9％,there was no statistically significant difference between two groups (x2 =0.97,P ＞ 0.05).Conclusion Effect of furosemide combined with intravenous injection of 50％ glucose in the treatment of congestive heart failure is reliable,the cardiac function recovered well,and has high application value.

In the treatment evaluation criterion of gastric cancer,overall survival(OS)is a dependable endpoint.Besides response cate(RR),disease-free survival(DFS),time to progression(TTP),progression free survival(PFS)have become major endpoints in the system.In addition,patient-reported outcomes(PRO)has received more and more attention.Biomarkers can also serve as a secondory endpoint.Adverse reaction may directly influence the continuity of the treatment and can not be neglected.The combined use of these endpoints can make the evaluation more objective.

Objective:A new method of measuring pulse wave velocity,double-cuff method,has been proposed based on oscillometric method and used to practice to validate its reliability in this paper.Methods:In this method,two cuffs are used to collect pulse wave signals of humerus and oar artery respectively.We analyse the two signals'time difference ?t by choosing wave boot as the pulse beating' start point.Pulse wave velocity can be calculated according to the formula PWV=?S/?t,in which ?S is the distance between humerus and oar artery and can be measured by a soft ruler.Results:The double cuff method,which has the advantages of simple operation,speedy measurement and low cost,can measure pulse wave velocity reliably,accurately,fast and simply.Conclusions:The pulse wave velocity measured by this new method,which can reflect the differences between normal people and critical or already hypertension suffers,is significant in forecasting hypertension.

Objective To investigate the distribution of pathogens in acute severe lower respiratory tract infection and drug resistance.MethodsFrom October 2014 to December 2016, selected 441 children patients with acute severe lower respiratory tract infectionin tianjinfifth centralhospital were drawn lower respiratory tract secretions from the oral endotracheal intubation of the disposable suction tube or container, bacterial culture and identification, to determine the type of bacteria, and drug susceptibility test.ResultsA total of 642pathogens were co-cultured in 441 children, of which 391 were Gram-negative bacteria, accounting for 60.90% (391/642), including Pseudomonas aeruginosa (17.45%), Acinetobacterbaumannii (14.80 %), Klebsiellapneumoniae (13.86%);Gram-positive bacteria were 150 strains, accounting for 23.36% (150/642), mainly including Staphylococcus aureus (15.58%).Gram-negative bacteria are mostly resistant bacteria and multidrug-resistant bacteria, piperacillin and imipenem resistance is relatively low.Gram-positive bacteria on penicillin, gentamicin, erythromycin and other drug resistance are high, resistance to vancomycin are low, including Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis Resistant drug resistance were 0.00%.ConclusionThe pathogens of lower respiratory tract infection in children with acute severe disease mainly include Pseudomonas aeruginosa, Acinetobacterbaumannii and Klebsiellapneumoniae and other Gram-negative bacteria, the clinical application should be based on the results of pathogen species and susceptibility test to choose reasonable antimicrobial drugs for children patients.

Objective:To do a psychometric test of PRCA-24(Personal Report of Communication Apprehension,24 items)to provide an instrument to detect the interpersonal communication anxiety of Chinese college students.Methods:The English version was translated into Chinese and back-translated.After checked the meaning of the original version and the back translation,data were collected from a sample of 208 college students from Dezhou College,including test-retest with two weeks interval.58 of the 208 received Social Avoidance and Distress Scale for criterion validity.Explorative factor analysis was used for item analysis and correlation analysis for criterion validity.Results:The revised scale was divided into three factors.Factor analysis indicated that the three factors could explain 53.8% of the variance with loading between 0.444~0.820.The coefficient of Cronbach's ? was 0.88.The test-retest correlations of the three factors were 0.66,0.73,0.79 respectively and that of the total scale was 0.85(P

As a kind of cytoskeleton protein,vimentin is a major member of intermediate filament family.Vimentin is found mainly in mesenchymal cells,and its expression and phosphorylation are regulated by many factors.In the past,vimentin was revealed as a scaffold maintaining cellular integrity,and recent studies shed lights on the evidence that vimentin played many important roles in cellular adhension,growth,apoptosis,cell signaling as well as inflammatory response.This article summarizes the recent progress of vimentin about its expression and function.Abstract:SUMM ARY As a kind of cytoskeleton protein,vimentin is a majormember of intermed iate filament fam i-ly.V imentin is found mainly in mesenchymal cells,and its expression and phosphorylation are regulated by many factors.In the past,vimentin was revealed as a scaffold maintaining cellular integrity,and re-cent stud ies shed lights on the evidence that vimentin played many important roles in cellular adhension,growth,apoptosis,cell signaling as well as inflammatory response.This article summarizes the recent progress of vimentin about its expression and function.

Objective To observe the retinal toxicity of repeated intravitreal injection with bevacizumab(Avastin)in diabetic rats.Methods Forty male Sprague Dawley(SD)rats were randomly divided into normal group(Group A,10 rats)and diabetes mellitus group(30 rats).The rats in diabetes mellitus group were induced with streptozotocin injection for diabetic retinopathy model.And then randomly divided into diabetic retinopathy(DR)group(Group B,10 rats),the rats were not intervened;the left eyes of the other 20 rats were intravitreal injected with bevaeizumab 3 μ1(25 mg/m1)for 3 times as experimental group(Group C);the right eyes of the 20 rats were not intervened as experimental control group(Group D),20 days after last intravitreal injection,retinal function was measured by Flicker Electroretinogram (F-ERG);retinal vascular pattern was determined by fluorescence microscopy of ethidium bromide(EB)stained retinal flat mounts;retinal morphological changes were determined by light microscope on hematoxylin-eosin (HE) stained sections;Thy-1 and VEGF expression was measured by immunohistochemistry staining.Results F-ERG showed that-the differences of a-and b-waves-the b-wave amplitude and the Ops-wave amplitude in the implicit time between group A,B,C and D were significant (F=33.165,36.162,19.955,23.243;P=0.000);the differences of a-wave amplitude between group A,B,C and D was not significant(F=0.097,P=0v961).Retinal blood vessel pattern was normalin Group A;retinal vascular vessels were tortuous and irregularly expanded in Group B:retinal vascular vessels of Group C were regular and thinner than Group A;microaneurysm were showed in Group D.Light microscope displayed that the layers of the rat retina of Group A were regular,the retinal architectures of Group B were irregular,the retinal layers were regular in Group C,the retinal layers were irregular in Group D.Immunohistochemistry staining discovered that Thy-1 and VEGF were mainly expressed in ganglion cell layer(GCL).Conclusion Repeated intravitreal injection of bevacizumab iS toxic tO retina of diabetes mellitus rats.

Objective To construct pEGFP-N1-TGF-β1 recombinant plasmid and transfect it into primary cultured neonatal piglet type Ⅱ alveolar epithelium cell (AEC-Ⅱ) by using lipofectamine 2000,in order to provide basis of methodology for producing recombinant plasmids for transplantation of transfected AEC-Ⅱ into ALI/ARDS animal model lungs.Methods PCR primers were designed to amplify the human TGF-β1 cDNA fragment from plasmid.XhoI and EcoRI were used for double digesting the empty plasmid pEGFP-N1 and cDNA fragment of human TGF-β1.Then the products of double enzyme digestion by using T4 DNA ligase were connected and transformed into DH5α and cultured over night for 16 hours.The structure of recombinant plasmid was identified by using PCR and base sequencing to verify the correctness of pEGFP-N1-TGF-β1 recombinant plasmid.It was then transfected into primarily cultured AEC-Ⅱ by lipofectamine2000 mediated transfection and cultured for another 48 hour.Plasmid DNA (pEGFP-N1-TGF-β1 recombinant plasmid) and lipofectamine 2000 were added into serum-free DMEM respectively,then DNA suspension and Lipofectamine 2000 suspension were blended together and added into cells.After 24-48 hours later,the expression level of enhanced green fluorescent protein (EGFP) was evaluated under fluorescence microscope.Results The structure of vector was verified as pEGFP-N1-TGF-β1 recombinant plasmid by using PCR and base sequencing.Green fluorescence found in some cells showed that the pEGFP-N1-TGF-β1 recombinant plasmids had been successfully transfected into primary cultured AEC-Ⅱ,however,the transfection efficiency still need tobe further improved such as repeating the transfection procedure once again or using adenovirus mediated transfection method to improve the efficiceny and to transplant the cells into animal lungs eventually.Conclusions pEGFP-N1-TGF-β1 recombinant plasmid was successfully constructed and,for the first time,transfected into primarily cultured AEC-Ⅱ of newborn piglets.This established method should be useful for investigation of therapeutic effect and outcomes of lung with experimental acute lung injury.