Animals ; Diabetes Mellitus, Experimental ; metabolism ; Diaphragm ; drug effects ; Lentinan ; pharmacology ; Male ; Mitochondria ; drug effects ; metabolism ; Nitric Oxide Synthase ; metabolism ; Rats ; Rats, Sprague-Dawley ; Succinate Dehydrogenase ; metabolism ; Superoxide Dismutase ; metabolism

China ; Occupational Exposure ; Threshold Limit Values ; Ultraviolet Rays

Adolescent ; Adult ; Female ; Humans ; Male ; Middle Aged ; Occupational Exposure ; Occupational Health Services ; Physical Examination ; statistics & numerical data ; Young Adult

Age Factors ; Aged ; Drugs, Chinese Herbal ; administration & dosage ; adverse effects ; Female ; Humans ; Male ; Middle Aged

Objective Laser induced fluorescence spectroscopy have been used in early diagnosis of GI cancer, but the device is expensive and inconvenient. The purpose of this study was to test the effectiveness and feasibility of a new device designed by the authors. Methods Two formulas were set up based on the analysis of OMA data and a new system was established. Fifteen samples of normal tissue and 40 cases of endoscopic diagnosed cancer were tested. Results (1) First formula: general energy of fluorescence was W 359～659 nm . (2) The second: the ratio of energy integral was W 359～460 nm /W 460～659 nm . (3) The sensitivity and specificity were 93.3% and 100.0% for 15 samples of gastro intestinal cancer in vitro and were 90.0% and 92.5% respectively for gastrointestinal cancer endoscopically. Conclusions (1) The deviec may help doctors to determine the characteristics of lesion in endoscopy and the efficacy of the instrument was the same as OMA system. (2) The cost was about one tenth of that for OMA system.

Relevant literatures on the relationship betw een fluorosis and oxidative stress were reviewed.Based on most of the original papers published in recent years,we can see the increased free radicals and oxidative stress may occur in certain stage of fluoride intoxication,but confirmation of the causality between oxidative stress and fluoride-induced damages still remains much work to do.

Objective To observe the preventive effects of Tripterygium hypoglaucum(Level)Hutch(THH)extract on T lymphocyte subpopulations in mice with graft-versus-host disease(GVHD)and to explore its protective mechanism.Methods 2?107 bone marrow cells mixed with 2?107 spleen cells from C57BL/6 mouse were transplanted into the myeloablative irradiated inbred BALB/c mouse.The experiment groups were designed as follows:model control group,cyclosporine A(CsA,10 mg?kg-1?d-1)group,THH(400 mg?kg-1?d-1)group,combination group(CsA 5 mg?kg-1?d-1 + THH 100 mg?kg-1?d-1).The ocurrence of GVHD was assessed by signs of weight loss,diarrhea,ruffled fur,hunched posture and histologic changes of skin,liver and small intestines.Chimerism was detected by monitoring the H-2b molecule in bone marrow cells of recipient mice with flow cytometer.The percentages of CD+3CD+4 and CD+3CD+8 T cells in peripheral blood were detected by flow cytometer.Results The survival time of CsA group,THH group and combination group were prolonged as compared with that of the model control group(P

Objective To evaluate the effects of root canal cleanliness on the fracture resistance of roots filled with AH-Plus. Methods Eighty single canal premolars were instrumented using step-back technique, then were randomly di-vided into four groups (n=20 for each group). Group A was washed with distilled water for 10 min, group B1 was washed with 5%EDTA for 1 min, group B2 was washed with 5%EDTA for 5 min and group B3 was washed with 5%EDTA for 10 min. Ten samples of each group were observed by scanning electron microscope at the coronal, middle and apical thirds to exam-ine smear layer removal. The remaining samples of each group were fixed into a electronic universal testing machine and ver-tically loaded until fracture. Results The difference of coronal and middle thirds was significant between group B3 and group B2 (P<0.05). At the middle third, there was significantly improved efficiency in smear layer removal in group B2 than that of group B1(P<0.05). The mean fracture resistance was significantly higher in group B3 (391.91±12.82)N than that of group B2 (335.54±16.14)N, group B1(296.47±17.82) N and group A (264.77±16.64)N (P<0.05). Group B2 showed a signifi-cantly better fracture resistance than that of group B1 and group A (P<0.05). Conclusion The complete removal of root ca-nal smear layer can significantly improve the fracture resistances of roots filled with AH-Plus.

Objective To investigate the value of contrast enhanced ultrasonography in evaluating cystic renal mass. Methods According to the imaging diagnosing standard, 29 cystic renal mass lesions were studied with contrast enhanced ultrasonography(CEUS), unenhanced ultrasonography (US), contrast enhanced CT(CECT) and then correlated their results with final histopathological results. The data were tested by receiver operating characteristic(ROC) curve. Results The area under ROC curve of CEUS and US was 0. 721, 0. 997, respectively. There was significant difference between the 2 groups (P=0. 003). The area under ROC curve of CEUS and CECT was 0. 997, 0. 997,respectively. There was no significant difference between the 2 groups (P= 1. 000). Conclusions CEUS is better than US and similar to CECT in evaluating cystic renal mass. CEUS with the Bosniak classification is useful for evaluating cystic renal mass.

Objective To evaluate the applicable value of two - dimensional speckle tracking imaging on assessing the local function and coronary collateral circulation of acute myocardial ischemia in dogs.Methods Open-chest model to induce acute myocardial ischemia in 10 dogs was established by ligating their anterior descending coronary artery(LAD). Two-dimensional dynamic gray- scale images of two standard left ventricular short axis views at the levels of mitral annulus and papillary muscle were acquired for the off -line comparative analysis before the operation and 0, 30, 60, 120, 240 and 360 minutes after the operation respectively. Peak systolic circumferential strain(CS), peak systolic radial strain(RS) and left ventricular fraction shortenting(LVFS) of 12 segments at the levels of mitral annulus and papillary muscle were analyzed with QLAB software. After the lab experiment,the fresh hearts of dogs were dyed by TTC.Results ①Compared with the preoperative value , the peak systolic CS and RS of ischemia myocardial regions(anteroseptum,anterior and lateral) in left ventricular short-axis decreased significantly( P ＜0. 05)during 30 to 60 minutes after ligating LAD. Sixty minutes later, the peak systolic CS and RS had a tendency to return to the level before the operation,although the statistical difference was existing. The peak systolic RS and CS also decreased in certain nonischemic regions (inferolateral and inferior). But CS and RS in inferoseptal regions didn't alter significantly. ②Compared with the preoperative value,LVFS in the ischemia regions were significantly decreased( P ＜0.05), while nonischemic regions had no significant difference. ③The results of TTC dyeing showed that the infarcted regions in left ventricular short-axis were anteroseptum, anterior and lateral with blood supplied by LAD, which were consistent with the results of RS and CS. Conclusions RS and CS,as the parameters of myocardial strain may reflect the range and extent of acute myocardial ischemia,and the strain changes of local myocardial segments after the construction of coronary collateral circulatory.