Objective To investigate the clinical efficacy of ultrasonic scaling combined with the vinegar-for treatment the patients with dental calculus.Methods 120 patients with dental calculus dental were randomly divided into two groups.Control group used ultrasonic scaling treatment,observation group was given ultrasonic scaling combined with the vinegar treatment.Results The clinical cure rate of the observation group was 96.67％ (58/60),the incidence rate of complications was 6.67％ (4/60).The clinical cure rate of the control group was 68.33％ (41/ 60),the incidence rate of complications was 21.67％ (13/60).There were statistically significant differences between the two groups about the cure rate and the incidence of complications (x2 =14.37,3.61,P ＜ 0.05).After treatment,periodontal pocket depth of the observation group was significantly lower than that of the control group (P ＜ 0.05).Sulcular fluid volume of the observation group was significantly lower than that of the control group (P ＜ 0.05).The difference of oral sample results of bacterial culture after treatment was statistically significant between the two groups (P ＜ 0.05).Conclusion The clinical effect of ultrasonic scaling combined with the vinegar in treatment patients with dental calculus is certain,which is worthy of further promotion.

Objective To study the feasibility, superiority and experience of minimally invasive laparoscopic-assisted surgery of the thyroid using ultrosonic scalpel. Methods In a period from January, 2006-August, 2008, 56 patients of the department of general surgery, the hospital of Heilongjiang Province were diagnosed as thyroid node. The size of the nodes ranged from 5.0 cm×4.2 cm to 3.5 cm×2.0 cm in uhrosonography. An incision of about 1.5-2.0 cm was made 1.0 cm above the suprasternal notch and the operation was performed with ultrosonic scalpel assisted with laparoscope. Partial thyroideetomy of lateral thyroid was performed in 20 cases, subtotal thyroidectomy in 25 cases, subtotal thyroidectomy of unilateral thyroid and partial thyroidectomy of contralateral thyroid respectively in 1 case, partial thyroidectomy of bilateral thyroid in 10 cases. Results All 56 cases have undergone minimally invasive laparoscopic-assisted surgery successfully in 50-146 min and no complications occurred. Fifty five cases were confirmed to be benign lesion pathologically. Conclusion Laparoscopic-assisted surgery of the thyroid is an effective with a good cosmetic result.

Objective To clarify weather or not lymph node dissection can achieve the requests of gastric carcinoma radical degrees under laparoscopic operation for advanced gastric careinoma. Methods Sixty-five cases of advanced gastric carcinoma with under 1/3 stomach lesions and three cases with middle 1/3 stomach lesions. were randomly divided into tow groups: assisted laparoseopic group(n=33)and conventional gastric carcinoma D2 radical surgery group(n=35).The number of lymph node dissection, and the incidence of positive lymph nodes oecnred in damaged capsule. the margin necrosis analyzed. Results The average lymph node dissection of the laparoscopic group were 20.79±5.21 were ohscrved. the positive oases were 9.63±4.64.The average lymph nnde dissection of the control group were 21.20±5.04.the positivecases were 9.63±4.64. The two groups had no significant difference f (P>0.05)1. Positive-margin lymph node in the control gmup was 6 and 14 in the experimenl group ( P>0.05). The experlment gnmp margin appeared necrosis under eletronic microseope and the control group appeared degeneratinn. Conclusions Laparoscopic advanced gastric carcinoma lymph node dissectinn can D2 radical of advanced gastriC carcinoma demands(D>N), have significant difference with conventional surgery. Positive lymph node dissection margin of treatment is better than traditional surgery.

Teaching resource sharing refers to the process and mode of sharing teaching resources among various universities.The problems existed are:traditional thought restriction,realistic system obstacles,defensive mentality of competitors etc.These problems can be resolved by establishing the highest leadership,setting up joint academic evaluation mechanism,setting up teaching operation monitoring institution,applying organizational management means (such as mobilizing more support form government).Meanwhile,these problems can be tackled by through achieving objectives of course sharing,exploring mode of course sharing,establishing responsibility system of course sharing and establishing credit parallel recognition mechanism.

BACKGROUND:Studies have shown that mesenchymsi stem cells (MSCs) participate in tumor stroma remodeling,but the mechanism is not clear.However,myofibroblast is related with tumor strema remodeling.OBJECTIVE:To investigate whether MSCs can differentiate into myofibroblast under the induction of tumor microenvironment.DESIGN,TIME AND SETING:This in vitro observation and in vivo transplantation cytology experiment was performed at institute of Low Temperature Medicine,Qilu Hospital of Shandong University from June 2006 to December 2007.MATERIALS:Twelve male New Zealand white rabbits,with 3-month-old,were purchased from Shandong Academy of Agricultural Sciences.METHODS:MSCs were obtained through bone marrow aspirates in male New Zealand rabbits and cultured by density gradient method.The 2~(nd) passage of MSCs were labeled with DAPI,and adjusted cell density to 5×10~(10) /L.The cells were assigned into experimental and control groups.For the experimental group,the passage two MSCs were cultured in low-glucose DMEM containing 10% fetal bovine serum and 30% VX2-cultured supernatant.For the control group,passage two MSCs were cultured in low-glucose DMEM containing 10% fetal bovine serum.Also,DAPI labeled self passage 2 MSCs of avery animal were delivered uniformly via direct surgical intratumoral injection to observe the differentiation of MSCs in tumor tissue.MAIN OUTCOME MEASURES:Immunofluorescense and RT-PCR were used to test the expression of α-SMA and Vimentin in MSCs of both groups 14 days later.At 3 weeks after VX2.intratumoral injection,Immunofluorescence was used to observe the distribution and differentiation of MSCs in tumor tissue.RESULTS:After stimulated with 30% VX2-cultured supematant for 2 weeks,Cy3 immunofluorescance showed that the expressions of α-SMA and Vimentin were strongly expressed in the experimental group,which was weakly expressed in the control group.Compared to the control group,the levels of α-SMA and Vimentin mRNA were obviously increased in the experimental group (P ＜ 0.01,P ＜ 0.05).At 3 weeks after MSCs intratumoral injection,these MSCs mainly distributed in tumor stroma and the expression of α-SMA and Vimentin in MSCs were also obviously increased,which demonstrated that the MSCs had differentiated into myofibroblasts.CONCLUSIONS:Under the induction of tumor microenivironment,MSCs can differentiate into myofibroblast,accordingly,accelerate the growth of tumor.

Objective To study molecular biology and clinicopathologic characters of peritoneal metastasis of advanced gastric cancer and their relationship to prognosis. Methods Based on the molecular biology and the clinicopathologic characters and prognosis single-factor analysis of relevant factors and risk assessment was made in 193 cases of gastric cancer.Results The younger the patient was with the earlier the time of occurrence of peritoneal metastasis of gastric cancer. There was a high expression of ICAM-1 and MMP-2,but low expression of TIMP-1 and p53 in gastric cancer patients with peritoneal metastasis.Conclusions ICAM-1,MMP-2,TIMP-1 and p53 can well predict the occurrence of metastasis and the prognosis of patients with gastric cancer,and these factors can be used for preoperative assessment of the development of metastasis of gastric cancer.

Objective To study the changes of NO plasma levels in patient with ischemic stroke(IS) of different types.Methods Using HNO 3 deoxidize enzyme method, we examined the levels of plasma NO in 230 patients with cerebral infarction, 103 patients with lacular cerebral infarction and 169 normal controls.Results In cerebral infarction group, the level of plasma NO was significant higher than the two other groups ( P

Objective: To investigate the dynamic changes in the content of endothelin(ET) in the plasma of coronary heart disease(CHD) patients and the relationship between myocardial function and ET.Methods: The plasma ET levels of 62 CHD patients were measured by immunoradiometric assay(IRMA) at 12 h,24 h,48 h,96 h and 7 d after the attack of CHD.The left ventricular ejection fraction(LVEF) was detected by echocardiography and the cardiac function was assessed according to the NYHA classification criteria.Results: The plasma ET level was significantly higher in the CHD patients than in the healthy controls(P

Objective To explore the clinical characteristics of neuromyelitis optica(NMO).Methods The clinical data of 18 patients with NMO were analyzed retrospectively.Results Of the enrolled patients,15 cases(83.3%) were relapsing form of NMO,16 cases(88.9%) were female and the mean onset age was 36 years old.Simultaneous bilateral optic neuritis(ON) or ON occured sequentially and horizontal impairent of spinal cord were the main clinic features in these 18 patients.Of the patients who received spinal cord MRI examinations,4 cases showed the lesions located in cervical cord,6 cases in thoracic cord and 5 cases in both cervical and thoracic cord.13 cases revealed lesions in spinal cord extended across 3 or more vertebral segments.Furthermore,brain lesions were detected by MRI in 6 patients.The mean onset number of per patient was five.Seven patients presented with mild disability,7 patients with median disability,2 patients with severe disability and death of 2 patients.Conclusions Most patients with NMO are female and relapsing form accounts for the majority type.The cardinal manifestations are bilateral ON and longitudinally extensive transverse myelitis(LETM).Some patients show brain lesions that may be detected by MRI examination.Relatively high relapsing frequency of clinical event and severe impairing are common in NMO.

Objective To determine whether plasma Leptin level is associated with atherosclerosis in maintenance hemodialysis(MHD)patients.Methods During 1997-01~2003-06,plasma Leptin levels in MHD patients and healthy volunteers were measured and intima-media thickness(IMT)and prevalence of atherosclerotic plaques of the extracranial common carotid artery were also measured.Results Plasma Leptin levels in the MHD patients were higher than those in the healthy volunteers(P