Objective To prepare the composite of Polycaprolactone(PCL) and Bone powder(BP) for bone repairing,and evaluate the biocompatibility of the material.Methods The materials were prepared in college of chemical engineering and materials science,Shanghai Jiaotong University,and the animal tests were fulfilled in Animal Experimental Center of the Second Military Medical University of PLA from June 2010 to March 2011.The PCL and BP were blended together to get the copolymer by haake ( Rheocord 900 ) torque rheometer.Then the extracts of the material in DMEM for 24 hours were obtained.Firstly biological safety detection of the materials was performed:①cytotoxicity test:the extracts were mixed with DMEM to seed rabbit osteoblasts for 24 hours.The cell proliferation was detected by MTT method.The cell proliferation rate and toxicity grate were also calculated ( cytotoxicity of grade 0 or 1 was qualified).②hemolysis test:the extracts were mixed with fresh rabbit blood,hemolysis rate were calculated ( hemolysis mte less than 5％ was qualified).③pyrogenic test:the extracts were injected into the rabbit ear vein with the dose of 10 ml/kg.The anus temperature was measured per hour for three times.The elevator degree was calculated by subtracting the natural temperature from the highest one.Secondly,cellular adhesion test of the material composite was performed.The composite was casted on the cover glasses.The samples were put in 6-well tissue culture plates,totally 6 × 104 osteoblasts were seeded each well and cultured for 3 days.The cells were observed at 24 and 72 hours of culture,and photography was performed using inverted phase contrast microscope.④general toxioity test:the extracts were injected into mouse vein with the dose of 50 ml/kg.The toxic symptoms were observed within 72 hours.Results The novel copolymer of PCL/BP was prepared.①Biological safety:the cell viability of the composite was 87.8％ and cytotoxicity was grade 1.The hemolysis rate was 2.4％,less than 5％.The maximal variety of body temperature in rabbits were 0.4℃,which accorded with the national standard ( ＜0.6℃ ).The general toxicity test showed that there were no adverse effects in the animals,such as death,convulsion,paralysis,respiratory depression,diarrhea and decreasing weight.②Cell adhesion:adhesive cells manifold obviously and assembled 24 hours later,inosculated gradually 3 days later,and the cells were in good fettle.Conclusion The composite of PCL/BP accords with cytotoxicity demand of the biological materials,presenting no toxicity,pyrogenicity or hemolysis.It possesses good biocompatibility and cellular adhesion.

Adult ; Broad Ligament ; Diagnosis, Differential ; Female ; Genital Neoplasms, Female ; diagnosis ; Humans ; Leiomyoma ; diagnosis ; Neoplasms, Multiple Primary ; diagnosis ; Ovarian Neoplasms ; diagnosis ; Teratoma ; diagnosis ; Uterine Neoplasms ; diagnosis

Objective To provide a mathematical formula and method for estimating the distance of shooting by uniform shotgun. Method 360 distribution graphs of canister were made on experimental target paper by shooting with No. 12 and No. 16 uniform shotgun (made in China), using No. 1 and No.3 pellets at the distance of shooting from 1m to 12m. The data of graph measuring and experimental distance were analyzed by mathematic theory. Results Six different types of mathematic formula were obtained for deduce the distance of shooting. They are: 12-1 type (choke) ss= -0.2 + 0.44d; 12- 1 type (no choke) ss= -0.94 + 0.63d; 12-3 type (choke) ss= -0.59 + 0.52d; 12-3 type (no choke) ss= -0.36 + 0.34d; 16-1 types= -0.37 + 0.30d; 16-3types= -0.16 + 0.23d. General software was made using Visual Basic 5.0 computer program. Conclusion This method can be used to deduce the distance of uniform shotgun shooting with little error.

Objective To discuss the condition in which ricochet bullet wound is formed, study its ballistic characters and ability to damage in the body, and establish features for the diagnosis of ricochet bullet wound. Method We shot four Yorkshire pigs by selecting No.54 pistol (made in China) and No.56 semiautomatic rifle with No.51 pistol bullet and No.56 rifle bullet, using quern stone as ricocheting target, copied 12 cases of ricochet bullet wound models and observed the characters of entrance wound, wound cavity and cartridge point. Results The formation of ricochet bullet lies with the properties of the target, the shape and velocity of the bullet, angle of incidence and angle of ricochet; the angle of ricochet formed by sharp bullet with high velocity is smaller than the angle by the round bullet with low velocity under the same circumstance; the wound does not produce an evident temporary cavity; six features for the diagnosis were established according to experimented results. Conclusion The correct diagnosis of ricochet bullet wound is of practical value to judging direction and distance of shot, trajectory reconstruction, analysing purpose of shot and evaluation of injury degree.

Objective To explore the clinical characteristics of eosinophilic gastroenteritis. Methods The clinical data of 2 children with eosinophilic gastroenteritis was retrospectively analyzed. Results Eosinophilia was found in both cases and path-ological examination indicated eosinophil infiltration in intestinal mucosa. The clinical symptoms were improved after hypoaller-genic diet, amino acid formula and anti-allergy treatment. Conclusions Eosinophilic gastroenteritis is characterized by eosino-phil infiltration with unknown pathogenic factor leading the treatment of eosinophilic gastroenteritis lacking of sound evidence.

China ; Gastroesophageal Reflux ; therapy ; Humans ; Integrative Medicine ; Medicine, Chinese Traditional ; methods

Adolescent ; Child ; Child, Preschool ; Clinical Trials as Topic ; Flow Cytometry ; Hematopoietic Stem Cell Transplantation ; Humans ; Immunophenotyping ; Leukemia, Myeloid, Acute ; diagnosis ; pathology ; therapy ; Neoplasm, Residual ; diagnosis ; pathology ; therapy ; Polymerase Chain Reaction ; Prognosis ; Recurrence ; Sensitivity and Specificity

Acute Kidney Injury ; chemically induced ; Anti-Infective Agents ; adverse effects ; Child, Preschool ; Humans ; Male ; Organometallic Compounds ; adverse effects

Anastomosis, Surgical ; adverse effects ; Brachiocephalic Trunk ; pathology ; Hemorrhage ; etiology ; therapy ; Humans ; Larynx ; surgery ; Male ; Middle Aged ; Postoperative Complications ; Trachea ; surgery ; Tracheal Stenosis ; etiology ; surgery ; Tracheotomy ; adverse effects

Objective:To observe the clinical effect of tuina plus foot bath with Chinese medicine for patients with diabetic foot (DF) in early stage.Methods:A total of 70 patients with early-stage DF were randomly allocated by the random number table into two groups,with 35 cases in each group.Patients in the control group received conventional medication,while patients in the observation group received tuina plus foot bath with Chinese medicine on the basis of conventional medication.The clinical efficacy was compared after 2 courses of treatment.Results:After treatment,intra-group comparisons of ankle-brachial index (ABI) showed statistical significance in both groups (both P＜0.05).The curative rate was 83.3％ in the observation group,with the total effective rate of 96.7％,versus 29.4％ and 76.5％ in the control group,respectively,and the between-group comparisons showed statistical significance (both P＜0.05),indicating a better effect in the observation group.Conclusion:Tuina plus foot bath with Chinese medicine has a good therapeutic effect for DF patients in early stage.