Objective: To evaluate the clinical effect of the double-orifice technique in the treatment of the anterior leaflet prolapse of mitral valve. Methods: 35 patients with severe anterior leaflet prolapse of mitral valve underwent valve repair using the double-orifice technique. The condition of valve was assessed with echocardiography preoperatively, intraoperatively, and postoperatively. Results: The mean postoperative valve area was (4 1?1 8) cm 2 against the preoperative valve area of (8 3?1 6)cm 2. Mild regurgitation was found in 2 patients after operation. There were no hospital deaths. At the latest follow-up, the heart function was improved significantly in all patients (NYHA class I). Echo-Doppler assessment of valve showed stable valve function in all patients. There were no late stenosis of valve and no late death. Conclusion: The results of this study show that the double-orifice technique is a safe and effective method for treatment of anterior leaflet of mitral valve with low mortality and morbidity, and excellent early and mid-term results.

Objective: To study the scavenging capacity of extract from four kinds of barley leaves on superoxide free radical and hydroxyl free radical. Methods: Measure the content of superoxide free radical and hydroxyl free radical by nitro blue tetrazolium(NBT) photo-reduction. Results: Along with the flavonoid in the reaction liquid, the scavenging rate shows ascending trend . When the concentration of flavonoid is 12?g/ml, its scavenging rate is 95.56% and 94.12% on superoxide free radical and hydroxyl free radical respectively. Conclusion: The flavonoids of barley leave s have stronger anti-oxidative action.

Animals ; Birds ; Hemagglutinin Glycoproteins, Influenza Virus ; genetics ; immunology ; Humans ; Influenza A virus ; genetics ; immunology ; pathogenicity ; Influenza in Birds ; immunology ; pathology ; virology ; Influenza, Human ; immunology ; pathology ; virology ; Virulence ; genetics

AIM: To investigate the expressions of the cyclin dependent kinase inhibitor (CKI) in human corneal epithelium.METHODS: The expressions of CKI, P27, P21 and proliferating cell nuclear antigen (PCNA) were tested in different regions of corneal epithelium by SP immunohistochemistry.RESULTS: Limbal basal cells stained positively for PCNA while central corneal epithelium cells stained negatively for PCNA, their difference was statistically significant (P＜0.01).Positive staining for P27 and P21 were observed in central epithelium, but there was no positive staining in limbal epithelium. Their difference were also statistically significant (P＜0.01).CONCLUSION: The different expressions of CKI P27, P21and PCNA in different corneal epithelial regions suggest that in limbal basal layer there are a group of cells that have higher proliferative capacity staying in G1 status, namely stem cell.

Objective In recent years , SIT has gradually become the main way for the treatment of allergic rhinitis .To con-trast the clinical efficacy of specific immune therapy for one year and two years of allergic rhinitis and discuss the appropriate mainte -nance treatment time of subcutaneous immunotherapy with Dermatophagoides pteronyssinus . Methods Ninety-eight patients with al-lergic rhinitis to Dermatophagoides pteronyssinus were allocated to receive either treatment for one year (A group, n=46) or treatment for two years(B group, n=52).Nasal symptoms and medication scores of two groups were assessed to evaluate the clinical efficacy af -ter treatment respectively , and evaluate the quality of life of patients . Results There was no statistical significance in nasal symptom and medication scores between the two groups after one year treatment (7.00 ±1.08 vs 6.63 ±0.79, 1.50 ±0.51 vs 0.41 ±0.42, P>0.05).The quality of life in patients with decreased , but lack of statistical significance (345.00 ±31.89 vs 344.42 ±32.26, P>0.05).It presented statistical significance in nasal symptom and medication scores between the two groups after two years treatment (6.20 ±0.78 vs 4.29 ±0.64, 0.53 ±0.43 vs 0.21 ±0.34, P<0.05).The quality of life between patients also presented statistical significance (P<0.05). Conclusion Compared with A group, B group can significantly alleviate the symptoms of allergic rhinitis , so we think the optimal maintenance treatment time of specific immunotherapy for allergic rhinitis should be at least 2 years.

OBJECTIVE: To explore the effects of Liangxue Huayu therapy (LXHYT), a traditional Chinese herbal therapy for cooling blood and dissipating blood stasis, on rat model of experimental autoimmune thyroiditis (EAT) and its impact on tumor necrosis factor-alpha and interleukin-10 mRNA expressions in peripheral blood mononuclear cells. METHODS: Forty Wistar rats were randomly divided into five groups: normal control group, untreated EAT group, cyclosporine A (CyA)-treated group, Tripterygium glycosides-treated group and Liangxue Huayu Recipe (LXHYR)-treated group. The interventions were given by gavage to the rats in different groups once a day. All rats were sacrificed after 4-week treatment, and the level of serum thyroglobulin antibodies (TgAb) and the changes of histological grade of thyroid specimen were assessed by blind evaluation. Expressions of tumor necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10) mRNAs in peripheral blood mononuclear cells were assessed by real-time reverse transcription polymerase chain reaction method. RESULTS: There was a significant increase in the serum TgAb level and severe inflammatory infiltration in the untreated group. Expression of TNF-alpha mRNA in peripheral blood mononuclear cells was increased, while the expression of IL-10 mRNA was decreased in the untreated group (P<0.01). Compared with the untreated group, CyA, Tripterygium glycosides and LXHYF could decrease the serum TgAb level (P<0.05), but the three interventions showed no significant improvement in thyroid inflammation (P>0.05). TNF-alpha mRNA expression was decreased, while IL-10 mRNA expression was increased as compared with the untreated group (P<0.05). CONCLUSION: LXHYT can decrease the serum TgAb level and recover the balance of Th1/Th2. This may provide an experimental basis for further research of assessing the antipsoriatic effect of Chinese herbal drugs with a rat model of EAT as an alternative model of psoriasis in vivo.

Objective To explore the effect of JiTT teaching-mode based on the Moodle on the undergraduate teaching of surgical nursing,and to reform the traditional undergraduate nurse teaching mode.Methods Totally 84 nursing undergraduates of Grade 2008 were randomized into the control group and the experimental group.The control group was given traditional teaching-oriented method,while the experimental group was applied JiTT teaching-mode based on the Moodle.Results In the experimental group,83.6％ recognized the JiTT teaching-mode,and 76.4％ felt satisfactory with the new teaching method.They thought the integrated check-up evaluation method and records distribution was more appropiiate.The nursing examination suggested that the average grade of the experimental group was significantly higher than the control group.In the experimental group,71.5％ thought the examination were less hard,though more study time was spending.6.7％ refused the new method because the higher requirement on the online study.78.4％ thought the in and out classroom study was more appropriate,it helped to cultivate the ability of the team work cooperation and the communication.Conclusions The JiTT teaching-mode based on the Moodle platform applying in the surgical nursing can construct an effective,communicative online course,stimulate the learning interest,cultivate the ability of exploration and cooperation,and also promote the learning revolution and high quality nurses cultivation.

Objective To investigate the effect of reinforcing spleen and kidney method on adriamycin-induced CKD in rats and to explore its possible mechanism.Methods Totally 36 Sprague-Dawley rats were randomly divided into a Adriamycin-induced model group and a control group.The model group was further divided into five groups:the Adriamycin-induced model control group,bennazepril-treated group,and TCM treated low,moderate,and high dose groups.The level of serum creatinine,urea nitrogen,24hours urine protein and urine creatinine were measured at 14,28,42 days after establishing the model rats.And the protein expression of transforming growth factor-β1 (TGF-β1) and cyclin-dependent kinase inhibitor p21cip1 (p21)were detected by immunohistochemistry.Results The proteinuria was observed on the seventh day after injection of adriamycin in adriamycin nephropathy model group,and reached summit on the fourteenth day.Both TCM treated groups and benazepril group reduced the level of urine protein within 24 hours (P＜0.05),the reduction was most remarkable in the TCM high dose group.The expression of p21 and TGF-β1 (p21 288627.66±97021.65,TGF-β1 98405.14± 19216.89) in kidney increased in the model groups,while the TCM treated high dose group (p21 518886.35±6810.89,TGF-β1 222012.95± 50484.73) was significantly lower than the model control group (P＜ 0.05).Conclusion Reinforcing spleen and kidney method could decrease the level of urine protein within 24 hours by regulating the expression of p21 and TGF-β1,so thus to protect renal function and delay progress of kidney disease.

Objective To approach the correlation between histology manifestation or Ki-67, p53 expression and superficial bladder cancer. Methods 54 cases of superficial bladder cancer were classified into two groups(with and without recurrence). The histological morphology was reviewed according to the 1998 WHO/ISUP classification. The expression of both Ki-67 and p53 was detected with immunohistochemical method, and a comparison was made between with- and without recurrence group. Results The average duration of follow-up survey for the patients was 32 months. Among the 54 patients, 28 got recurrence. Of the recurred cases, 5 were papillary urothelial neoplasm of low malignant potential (PUNLMP), 14 were low grade papillary urothelial carcinoma (LGPUC), and 9 were high grade urothelial papillary carcinoma (HGPUC). While among the 26 cases without recurrence, 4 were papilloma, 12 were PUTLMP, 8 were LGPUC, and 2 were HGPUC. The recurrence rate was 29.4% (5/17) in PUTLMP, 63.6% (14/22) and 81.8% (9/11) in LGPUC and HGPUC, respectively. There was a significant difference in Ki-67 and p53 expression between the recurrence and non-recurrence groups, the positive rate in recurrence group was significantly higher than that in non-recurrence group (P

Objective To investigate the correlation between caspase-3 and left ventricular function at different phases after ischemia-reperfusion. Methods Totally 36 wistar rats were randomly divided into Sham operation group and ischemia-reperfusion(IR)group. The rat ischemic cardiac region of IR group was taken after 1, 3, 6, 12 and 24-hour reperfusion followed to 30-minute ischemia and detected for the activity fragment of caspase-3 P20, the activity of caspase-3, myocardial apoptosis index, left ventricular function (LVF) and creatine kinase-MB (CK-MB) respectively. Results With reperfusion time prolonging, the activity fragment of caspase-3 P20 increased, the activity of caspase-3, myocardial apoptosis index, left ventricular function and CK-MB all changed. The activity fragment of caspase-3 P20 and CK-MB peaked at 6 h, and the activity of caspase-3 and myocardial apoptosis index at 12 h, and then maintained platform; LVSP and ?dp/dt_max increased while LVEDP decreased gradually with the reperfusion time prolonging. All indexes mentioned above of IR group had statistical significance as compared with that of Sham operation group(P