Objective To investigate the relationship between primary hepatocellular carcinoma (HCC) with multiple nodules and short-term recurrence after radical resection.Methods We retrospectively analyzed the clinical data and follow-up of 692 consecutive HCC patients who underwent curative resection at the Affiliated Hospital of Qingdao University from January 2000 to December 2011.The diagnosis of multiple nodules was based on preoperative imaging data and observations in operation.Results Of 692 HCC patients, 126 patients had multiple nodules (18.2％, the MN group) and 566 patients had a single nodule (71.8％, the SN group).The 1-, 2-, 5-, and 10-year overall survival (OS) rates were 86.5％,72.2％, 38.1％, and 23.2％ for the MN group, and 93.8％, 82.7％, 58.2％, and 35.7％ for the SN group, respectively (P ＜ 0.05).The 1-, 2-, 5-, and 10-year disease-free survival (DFS) rates were 68.6％, 46.8％, 27.2％, and 19.8％ for the MN group, and 80.2％, 62.1％, 42.0％, and 34.6％ for the SN group, respectively (P ＜ 0.05).Cox regression model analysis showed a maximum nodule diameter of ＞ 5 cm was an independent risk factor of short-term recurrence in the MN group.Conclusions The prognosis of HCC patients with multiple nodules after radical resection was poorer and the short-term recurrence rate was significantly higher than those patients with a single-nodule.A maximum nodal diameter of ＞ 5 cm was an independent risk factor of short-term recurrence for patients with multiple nodules after radical resection.Therefore, patients with multiple nodules, especially ＞ 5 cm, should be followed up closely and they are good candidates for a multiple disciplinary team (MDT) treatment.

This paper summarizes the main methods of civil-military integration,academy of military medical sciences,analyzes the main problems restricting the development of military and civilian integration depth,puts forward to strengthen military medicine civil-military integration of science and technology innovation and development measures.

Objective To report the results of the surgical treatment of cervical spondylotic myelopathy(CSM) secondary to athetoid cerebral palsy and clarify the characteristics and the principle of treatment of the disease. Methods The age of the patient, segments of vertabrae involved and operated upon, results and complications of 32 cases with CSM secondary to athetoid cerebral palsy were statistically analyzed and compared with 93 patients who had isolate CSM operatively treated at the same time. Results 1)The average age of the patients in the group of CSM secondary to athetoid cerebral palsy was 37.94 years,and in the group of isolate CSM was 52.48 years, with significant statistic difference(P

Objective:Cluster and discriminant analysis of the morphological characters of Angle's Class Ⅱ malocclusion was performed using Procrusts standardization.The classification of craniofacial morphology with the help of morphometry and multivariate statistics was also discussed.Methods: A total of 894 class Ⅱ patients were collected from the orthodontic department,Peking University School and Hospital of Stomatology during 1997-2000.Using Procrusts standardization and cluster analysis,the samples were divided into different groups,discrimination equations were then established.Results: The samples were divided into 11 subdivisions by Procrusts superimposition and cluster analysis.Three discrimination equations were established.The accuracy rate of cross-validated grouped cases was 80.17%.Conclusion: Procrusts standardization had certain advantages in morphological classification;cluster analysis could be used in classification of Angle class Ⅱ malocclusion;For different types,the differentiate rate was not the same;the discrimination equations was the foundation for future research.

OBJECTIVE To investigation central venous catheter-related infections(CRI) in critical patients. METHODS Fifty-nine cases from Jul 2006 to Jun 2007 in intensive care unit (ICU) of our hospital were analysed retrospectively. RESULTS Among 59 cases, 27 cases were with positive results of fungi (51.85%) and bacteria(48.15%) in which G+(25.93%) and G-(22.22%). CONCLUSIONS CRI is related to the indwelling time, fungi are the major pathogens. It is important to prevent CRI through careful nursing. We must pull out the catheters if recessary and antibiotics should be used reasonablly.

Adolescent ; Epistaxis ; etiology ; Histiocytosis, Sinus ; complications ; diagnosis ; Humans ; Male

Objective To study the relationship between essential hypertension(EH)with serum homocysteine(HCY),uroten-sinⅡ(UⅡ),angiotensin converting enzyme(ACE)and N-terminal pro-brain natriuretic peptide(NT-proBNP).Methods By collec-ting the clinical cases,UⅡwas determined by ELISA and HCY,ACE and NT-proBNP were simultaneously detected by ELISA.The detection results were analyzed and compared between the patients with essential hypertension(EH group)and the healthy con-trols.Results The levels of serum HCY,UⅡ,ACE and NT-proBNP in the EH group were significantly increased compared with the healthy control group;the area under curve (AUC)of serum HCY,UⅡ,ACE and NT-proBNP in the ROC curve in the EH group were 0.93,0.765,0.792 and 0.972 respectively,which showed clinical diagnostic significance.Conclusion The levels of HCY,UⅡ,ACE and NT-proBNP are highly expressed in EH and have significant differences compared with the healthy popula-tion,which has the diagnostic value to EH.

Objective:To characterize the volatile compounds in 10 batches of disposable infusion sets and 6 batches of nasal can-nulas by GC-MS and determine the main odor-active compounds. Methods:The volatile components were extracted using a headspace sampler. An HP-5MS capillary column (30 m × 0. 25 mm,0. 25 μm) was adopted, and the qualitative analysis was performed by total ion chromatography ( TIC) of full scan with temperature programmer. Results:A total of 19 major volatile compounds were identified, which were hydrocarbon, alcohol and carbonyl compounds (such as aldehyde and ester). Based on the combination of odor test and GC-MS, the concentration of alcohol compounds (2-ethyl hexanol, 2-EH) had the most notable effect on the odor of samples. Conclu-sion:The samples with unacceptable order contain 2-EH with relatively high content, which should be paid more attention.

Aim To establish a hepatic carcinoma cell line with stable voltage-gated chloride channel 3 ( ClC-3 ) gene silencing through the lentivirus-mediated short-hairpin RNA ( shRNA ) method and investigate the effects of gene silencing on invasion and migration. Methods Three lentiviral vectors coding shRNA tar-geting ClC-3 gene were constructed, the recombinant plasmids were packaged into mature lentivirus by 293FT cells, and then the lentiviruses were harvested, concentrated and titrated. MHCC97H cells were infec-ted with the recombinant lentiviruses and then were se-lected to obtain cell lines stably expressing ClC-3 shR-NA. The efficiency of ClC-3 mRNA and protein ex-pression interference were determined by real-time flu-orescence quantitative PCR and Western blot, respec-tively. The effects of ClC-3 gene interference on inva-sion and migration of MHCC97 H cells were performed by Transwell chamber assays with or without Matrigel and cell scratch assay. Results The recombinant lentiviral vectors were successfully constructed and four lentiviruses were acquired after packaged by 293 FT cells. One negative control cell line and three cell lines with ClC-3 gene interference ( MHCC97 H/shClC-3-1 , shClC-3-2 and shClC-3-3 ) were successfully construc-ted after MHCC97 H cells were infected with lentivirus-es. The expression level of ClC-3 mRNA and protein in three ClC-3-silenced cells were obviously lower than the negative control cells ( P <0. 01 ) , MHCC97 H/shClC-3-2 cells showed the greatest inhibition of ClC-3 mRNA and protein expressions. As compared with the negative control cells, the ClC-3 gene interference sig-nificantly decreased invasion and migration of MH-CC97 H cells in vitro ( P <0. 01 ) . Conclusion The hepatic carcinoma cell lines with stable ClC-3 gene si-lencing were successfully established and the ClC-3 gene interference could significantly inhibit invasion and migration of MHCC97H cells.

BACKGROUND: It has been verified in relevant clinical and epidemiological researches in recent years that a part of stomatopathy is associated to psychological stress factors.OBJECTIVE: To analyze the relationship between the changes in salivary secretory IgA and cortisol and psychological stress states.DESIGN: cross-sectional investigation was designed.SETTING: Department of Stomatology, Zhejiang University.PARTICIPANTS: The investigated participants were 60 students in the 2nd grade of Fuzhou №.2 Senior School selected from May to November 2003. Fifty-seven of them accomplished the investigation, of male; aged varied from 17 to 19 years, of healthy, no stomatopathy and family history of disorders in immune system and without indulgence in smoke and alcohol. All of them had participated in the 3-day terminal exam.METHODS: Before the exam, evaluation with symptom checklist (there are 90 questions, including about 10 factors, such as somatization, compulsive state, sensitive interpersonal relationships, melancholy, anxiety, hostility, fear, paranoid state, psychotic state and others. The total average score results by divided total score with 90. ) was applied. Fifty-seven students that had accomplished the investigation were divided in low-symptom group (the total score of symptom checklist ＜ 2.0) and high-symptom group (the total score ≥ 2.0). 5 weeks before the exam, the students were called together to fill up investigation form of general situation. Four weeks before the exam and at 7: 30 am on the day of exam, the first and second saliva specimens were collected respectively to determine salivary secretory IgA and cortisol before and after exam stress. The students were called together again 3 days after exam to fill up questionnaire on psychological health to evaluate their psychological health state.stress.RESULTS: Intention management analysis was adopted, in which 57 stulist with Chinese norm: after exam, the scores of 4 factors, named compulsive state, anxiety, hostility and paranoid state, were higher than Chinese norm [(1.98±0.72, 1.69±0.61) scores; (1.79±0.31, 1.42±0.43) scores; (1.77±0.68,1.50±0.57)scores; (2.00±0.71, 1.52±0.60)scores, P ＜ 0.01] and there was parison of salivary secretory IgA content after stress: that before exam in low symptom group was remarkably higher than that in high symptom group [(236.6±82.29),(194.55±66.29) mg/L, P ＜ 0.05], that after exam in low symptom group was higher than that in high symptom group [(182.21±55.92,tration after stress: that after exam in low symptom group was remarkably higher than that in high symptom group [(5.96±2.46, 3.68±2.26) μg/L, P secretory IgA and cortisol in two groups: the decrease rate of secretory IgA (0.012±0.197) and increase rate of cortisol (0.199±0.654) were relevant to compulsive state and paranoid state.CONCLUSION: In evaluation with symptom checklist after exam, the scores of anxiety, compulsive state, hostility and paranoid state were higher than Chinese norm, indicating that exam stress induces increased anxiety level and variation of psychological behavior of students. The difference presented in decrease rate of salivary secretory IgA before and after exam stress between high and low symptom groups, explaining that the students with more psychological symptoms are weak to endure exam stress.