1.Mitochondrial calcium uniporter complex and regulation of their protein interaction
Chinese Journal of Pathophysiology 2015;(12):2291-2295
Mitochondrial calcium homeostasis plays a key role in maintaining various cellular characteristics and mediating cellular physiological function and pathological processes .Although it has long been known that mitochondria takes up Ca2+, the molecular identities of the channels and transporters involved in this process are revealed only recently . Here, we review the structure and function of the channel-forming subunit, mitochondrial calcium uniporter (MCU) and its regulators, which include MICU1, MICU2, and MCUR1.
2.Clinical value of three-dimensional laparoscopic radical gastrectomy for gastric cancer
Chinese Journal of Digestive Surgery 2016;15(9):888-891
Objective To investigate the clinical value of three-dimensional (3D) laparoscopic radical gastrectomy for gastric cancer.Methods The retrospective and descriptive study was adopted.The clinical data of 28 patients who underwent 3D laparoscopic radical gastrectomy for gastric cancer at the First Affiliated Hospital of Nanjing Medical University from October 2014 to March 2016 were collected.Trocar placement and surgical procedures were the same as 2D laparoscopic radical gastrectomy.Observation indicators included (1) surgical situations:operation time,volume of intraoperative blood loss,number of lymph node dissected,(2) postoperative situations:time for out-of-bed activity,time to anal exsufflation,time for fluid diet intake,occurrence of complications and duration of hospital stay,(3) follow-up situations.The follow-up was performed to detect postoperative survival of patients and tumor recurrence and metastasis by outpatient examination and telephone interview up to May 1st,2016.Measurement data with normal distribution were presented as x ± s.Results (1) Surgical situations:all the 28 patients underwent successful 3D laparoscopic radical gastrectomy,without conversion to open surgery and perioperative death.Seven patients received 3D laparoscopy assisted total gastrectomy with Roux-en-Y esophagojejunostomy,with operation time of (214 ± 47)minutes.Twenty-one patients underwent 3D laparoscopic distal gastrectomy with uncut Roux-en-Y gastrojejunostomy,with operation time of (181 ± 27) minutes.Volume of intraoperative blood loss and number of lymph node dissected in 28 patients were (53 ± 29)mL and 34 ± 10.(2) Postoperative situations:time for out-of-bed activity,time to anal exsufflation and time for fluid diet intake were (54 ± 17) hours,(77 ± 16) hours and (5.0 ± 1.1) days,respectively.Of 28 patients,1 complicated with gastrointestinal hemorrhage was cured by conservative treatment,and other patients had no complications.Duration of postoperative hospital stay was (8.8 ± 1.6) days.(3) Follow-up situations:all the 28 patients were followed up for 2-18 months with a median time of 10 months,without occurrence of tumor recurrence and metastasis.One patient undergoing 3D laparoscopic distal gastrectomy with uncut Roux-en-Y gastrojejunostomy was complicated with afferent recanalization,without obvious discomfort,and received regular gastroscopy reexaminations to detect bile reflux.Conclusion 3D laparoscopic radical gastrectomy for gastric cancer is safe and feasible,with good short-term outcomes.
3.Relationship between forkhead box Q1 and tumors of digestive system
Chinese Journal of Hepatobiliary Surgery 2016;22(5):351-354
Forkhead box Q1 (FoxQ1) is a transcription factor protein with the function of cell differentiation regulation.Recently,increasing evidence has demonstrated that FoxQ1 is significantly associated with pathogenesis of digestive system tumors.This article systematically reviewed the expression and the role of FoxQ1 in the pathogenesis of digestive system tumors.Potential therapeutic target of FoxQ1 was discussed as well.
4.Study of implementation of overseas elective posting program for medical student at abroad
Chinese Journal of Medical Education Research 2013;(8):826-828
As a basic teaching method in the medical education system represented by British and USA, overseas elective posting for medical student is playing significant role in enriching medical students’ internship experience as well as improving their comprehensive knowledge and skills. Besides it’s also essential for cultivating modern and high-quality medical talents in line with international stan-dard. Therefore the purpose of the paper is to provide some references for carrying out international ex-change at students’ level smoothly in domestic medical universities and colleges by simply introducing some good experiences and measures from several typical foreign medical colleges.
5.Study on the polymorphism of HLA-DQA1 gene in hepatitis B patients of Uygur and Han nationality lived in Xinjiang region
Chongqing Medicine 2016;(1):1-4
Objective To investigate the relation between the human leucocytes antigen-DQA1(HLA-DQA1) alleles poly-morphism and the Uygur Han nationality hepatitis B patients in Xinjiang and the genetic susceptibility healthy controls ,which is provide some important clues to seek the susceptible genes and disease-resistant genes of HBV infection for Uygur and Han nation-ality hepatitis B patients .Methods HLA-DQAl alleles of 182 cases of the Hepatitis B patients and 163 people were compared with HBV DNA and ALT level .HLA-DQA1 * 0102 ,-DQA1 * 0104 ,-DQA1 * 0201 ,-DQA1 * 0301 ,-DQA1 * 0302 ,-DQA1 * 0501 genes frequency are detected with PCR-SSP .Results Compared the Uygur hepatitis B patients ALT abnormal and HBV DNA high copy quantity group with healthy controls group in allele′s frequency analysis found that HLA-DQA1 * 0301 ,-DQA1 * 0501 genes had statistic significance(P< 0 .05) .Compared the Han nationality hepatitis B patients ALT abnormal and HBV DNA high copy quanti-ty group with healthy controls group in allele′s frequency analysis ,It was found that HLA-DQA1 * 0102 ,-DQA1 * 0201 ,-DQA1 *0301 genes difference had statistic significance(P < 0 .05) .HLA-DQA1 * 0102 had the statistic significance between high and low copy quantity groups(P< 0 .05) .Compared the Han nationality hepatitis B patients ALT normal and low copy quantity group with healthy controls group in allele′s frequency analysis ,It was found that HLA-DQA1 * 0201 genes had statistic significance(P <0 .05) .HLA-DQA1 * 0102 ,-DQA1 * 0301 had statistic significance between the Han and Uygur nationality for HBV patients(P<0 .05) ;HLA-DQA1 * 0201 ,-DQA1 * 0501 had statistic significance between the Han and Uygur nationality for healthy people(P<0 .05) .Conclusion HLA-DQA1 * 0501 is the protected gene of Uygur hepatitis B patients ;-DQA1 * 0301 is the susceptibility gene .The Han nationality hepatitis B patients group HLA-DQA1 * 0102 ,-DQA1 * 0301 ,-DQA1 * 0302 is the susceptibility genes and -DQA1 * 0201 is the Antagonism gene .
6.Expression of human membrane associated sialidase gene in prostate carcinoma PC-3 cell line.
Chinese Journal of Applied Physiology 2005;21(3):299-304
Cell Line, Tumor
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Genetic Vectors
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Humans
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Male
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Neuraminidase
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genetics
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metabolism
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Prostate
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metabolism
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Prostatic Neoplasms
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metabolism
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pathology
7.Design and analysis of the scientific technological retrieval management system of TCM
Li KANG ; Kunjie YANG ; Fengling LI ; Yang YANG ; Deli YANG ; Jun XU ; Haomiao XU
International Journal of Traditional Chinese Medicine 2012;34(5):436-438
This paper expounds the needs analysis and system design of traditional Chinese medical science and technology of sci-tech novelty search management system,discusses the architecture of the system and each functional module,and summarizes and discusses the meaning of this system when applied in sci-tech novelty search.
8.The value of serum albumin level for the prognosis of late-preterm infants infection
Chunyan YANG ; Baoyun LI ; Ping XU ; Yujun YANG ; Qiaozhi YANG
Chinese Journal of Neonatology 2015;30(3):196-199
Objective To study the value of serum albumin ( ALB ) level for the prognosis of late-preterm infants infection. Methods Late-preterm infants admitted to the neonatal intensive care unit ( NICU) from July 2012 to July 2013 were recruited and their clinical data retrospectively reviewed, including the laboratory examination results, neonatal critical illness scores ( NCIS ) , perinatal complications and prognosis. The infants were assigned into three groups based on ALB levels (>30 g/L, 25-30 g/L, <25 g/L). Results A total of 257 cases were recruited and 122 cases (47. 4%) had ALB levels <25 g/L. 32 had neonatal sepsis ( sepsis group) , 190 neonatal infection ( infection group) and 35 without infection ( no-infection group ) . The incidences of hypoalbuminemia among these groups were 84. 4%, 50. 0% and 28. 6%, with the mortality rate 15. 6%, 0. 5% and 0%. The incidence of hypoalbuminemia and mortality rate in sepsis group were significantly higher than the other groups ( P<0. 05 ) , and no statistically significant differences between infection group and no-infection group ( P<0. 05). The ALB level in survived infants [(29. 6±7. 5)g/L] was statistically higher than the deceased ones [(20. 4±6. 9)g/L](P<0. 05). The incidence of critically ill newborns was 65. 5% in ALB <25 g/L group, significantly higher than the other groups (P<0. 05). 26. 2% in ALB <25 g/L group had more than 4 organs injuries, significantly higher than ALB >30 g/L group ( P<0. 05 ) . Conclusions Hypoalbuminemia is common among neonates with sepsis. The ALB level had predictive value for the prognosis of neonatal infection.
9.Study on Price Management Mechanism of Medicines in Hong Kong Based on the Value Chain of Medicine
Wei XU ; Shuang YANG ; Mengjiao LI
China Pharmacy 2016;27(1):4-7
OBJECTIVE:To provide reference for the price management of medicines in Chinese mainland by inquiring into rel-ative management in Hong Kong. METHODS:Based on the steps of price management and the value chain of medicines in Hong Kong,the management mechanism of medicine pricing,circulation and compensation were researched. RESULTS & CONCLU-SIONS:The prices of the medicines in Hong Kong are mainly determined by the tender between Hospital Authority and the pharma-ceutical suppliers. In circulation,medicine payer as main body in bidding and purchase conducthigh qualitybidding andmasspurchase,and price remained the same in public hospital. On the basis of the purchase price,the price that actually paid by pa-tients is the result of government compensation. Chinese mainland can draw lessons from such drug bidding mode in Hong Kong which will lead health care department to act as the main body in bidding,weaken the relationship between the hospitals and the drug dealers or manufacturers,and expand the government's financial investment in public medical institutions to improve reim-bursement.
10.Study on the Price Management Mechanism of Medical Services in Macau of China
Wei XU ; Shuang YANG ; Mengjiao LI
China Pharmacy 2016;(4):449-451
OBJECTIVE:To explore price management mechanism of medical service in Macau of China,and to provide refer-ence for the formulation of price management mechanism of medical service in the mainland area of China. METHODS:From two key links of pricing and compensation,price management mechanism of medical service in Macau was introduced. RESULTS &CONCLUSIONS:In respect of pricing,Macau Health Bureau as pricing subject set up guide price for public health institutions by marginal cost pricing,which focused on technology value and labor value of medical staff,and graded pricing for health institu-tions at various levels. In respect of compensation,compensation subjects included government,social security funds,charitable or-ganization,etc. The government provided fixed-rate compensation for citizen by allocating funds for public health institutions,so as to improve compensation level for primary health institutions. The social security funds extended sick benefit to insured patients, and compensated patients at fixed rate. Charitable organization subsidized private health institutions to reduce medical service price. Finally,actual price has been formulated through pricing and compensation links,and the experience is worthy of learning by the mainland of China.