Objective To discuss the practice of nursing management model of stroke unit based upon the international standards implemented in the neurological unit.Methods The experiences of the stroke unit management were summarized and applied to instruct the clinical practice.Results After the implementation,the average hospitalization days,the proportion of drugs,medical cost,and mortality rate decreased.The satisfactory degree of patients with the stroke unit increased.Conclusions The nursing management model of stroke unit not only improved the quality of nursing service to stroke patients,but also decreases the mortality and disability rate,shortened the length of hospital stay,and enhanced the satisfaction degree of patients and their family members.

Objective To study the clinical efficacy of treatment of atrophic scars of facial hemangioma after isotopes radiotherapy with micro-plasma.Methods A total of 52 patients with atrophic scars were selected under micro-plasma treatment for 4 times,one time every six weeks.And their efficacies were evaluated by compared with single standard of that before and after the treatment.Results By means of rate of clinical index,the total effective rate of atrophic scars was 82.7％.The degree of improvement was as follows:cured in 53.8％,marked effective in 28.9％,effective in 17.3％ and no case ineffective.The total effective rate of hyperpigmentation was 90.3％,including cured in 59.6％,marked effective in 30.8％,effective in 9.6％ and no ineffective cases.Conclusions Micro-plasma is an effective therapy for atrophic scars of facial hemangioma after isotopes radiotherapy,which can significantly improve the depression degree of scars,eliminate the hyperpigmentation and improve the color of scars,but it only has adverse reactions of wound pain and postoperative wound erythema.

Objective To compare efficacy of indirect immunofluorescence (IFA) and real‐time fluorescent polymerase chain re‐action(PCR) in detection of mycoplasma pneumonia in children .Methods A total of 137 children clinically diagnosed as Mycoplas‐ma pneumoniae(MP) infection were selected and divided into groups by age ,including <1 years old group(35 cases) ,1- <5 years old group(69 cases) and 5-15 years old group(33 cases) .Blood specimen and throat swabs were collected and detected by using IFA and real‐time fluorescent PCR .At the same time ,all of the selected children were treated with conventional therapy ,according to total effective rate ,positive coincidence rates of the two methods were statistically analysed by age .Results The positive coinci‐dence rates in children with MP infection <1 years old and 1- <5 years old detected by using real‐time fluorescent PCR were high‐er than that detected by using IFA ,while among children 5-15 years old ,the positive coincidence rate was higher detected by using IFA compared with that detected by using real‐time fluorescent PCR ,all had statistically significant differences (P<0 .05) .The o‐verall positive coincidence rates of the two methods were not significantly different(P>0 .05) .Conclusion IFA and real‐time fluo‐rescent PCR both could be used as effective methods for detecting MP ,but there are some differences of detective efficacy between the two methods in each age group .Therefore ,it is suggested that for children under 5 years old real‐time fluorescent PCR might be selected ,for children aged 5 years old and over IFA might be selected ,in order to improve the detection accuracy and provide better guidance to clinical medication .

There are 13 members of the Let -7 miRNAs family which is regarded as tumor suppressor gene, locating in nine different chromosome loci. Lin28 acts as negative regulatory factor of miRNA biological recurrence. By selectively blocking the processing synthesis of the Let-7 miRNAs family, Lin28 block the inhibition effect of miRNA of proto-oncogenes and interact with RNA helicase to enhance gene translation at the same time. By not quite clear mechanism, an up-regulation of Let-7 inhibits the expression and function of Lin28. In more and more studies of human tumor, Lin28/Let-7 axsis was proved to be important significance of the tumor's occurrence and development. In this paper, we research briefly the recent progress of the molecular mechanism and related influence factors of ILin28/Let-7 axsis.
Animals ; Humans ; MicroRNAs ; RNA-Binding Proteins

Bacillus amyloliquefaciens B10-127 was used to produce 2,3-butanediol (2,3-BD) from residual glycerol obtained from biodiesel synthesis. Important variables for 2,3-BD fermentation, pH and dissolved oxygen, were studied. When pH was maintained constant, the yield of 2,3-BD was inhibited. The highest 2,3-BD yields were achieved by fermentation without any pH control with an optimized initial pH 6.5. Batch fermentative production of 2,3-BD by B. amyloliquefaciens was investigated using various oxygen supply methods by changing agitation speed. Based on the analysis of three kinetic parameters including specific cell growth rate (micro), specific glucose consumption rate (q(s)) and specific 2,3-BD formation rate (q(p)), a three-stage agitation speed control strategy was proposed, aimed at achieving high concentration, high yield and high productivity of 2,3-BD. Maximum concentration of 2,3-BD reached 38.1 g/L, with the productivity of 1.06 g/(L x h), which were 14.8% and 63.1% over the best results from constant agitation speeds. In a pulse fed-batch fermentation, 2,3-BD concentration and productivity were significantly improved to 71.2 g/L and 0.99 g/(L x h), respectively. To our knowledge, these results were the highest for 2,3-BD production from biodiesel-derived glycerol.
Bacillus ; classification ; metabolism ; Biofuels ; analysis ; Bioreactors ; Butylene Glycols ; metabolism ; Fermentation ; Glycerol ; metabolism ; Hydrogen-Ion Concentration ; Industrial Microbiology ; Oxygen ; analysis

Objective:To observe the effect of Livostin spray on children＇s allergic rhinitis and to search the mechanism of treating allergic rhinitis. Method: 113 patients were treated with Livostin spray (Livostin group) or normal saline spray (control group). Result:The total efficiency of Livostin group in treating allergic rhinitis is above 95.1％ and that of the control group is 25.0％. Initial time of starting effect of Livostin (72.1％) is in 1 minute, and that of the control group (mostly 23.1％) is in 3 minutes. The keeping curativeeffect time of Livostin spray is mostly (72.1％) above 5 hours and that of the control group is mostly (30.8％) in 3 hours. After 2 weeks,the eosinophilic granulocyte number in nose＇s secretion of Livostin group is obviously reduced (P＜0.05). Conclusion: Livostin is better than control group in relieving symptoms, keeping curative effect and safety,so Livostin is one kind of effective drug in treating children＇s allergic rhinitis.

Tooth eruption is a series of complicated physiological processes occurring once the crown is formed completely, as well as when the tooth moves toward the occasion plane. As such, the tooth moves through the alveolar bone and the oral mucosa until it finally reaches its functional position. Most studies indicate that the process of tooth eruption involves the alveolar bone, dental follicles, osteoclasts, osteoblasts, and multiple cytokines. Dental follicles regulate both resorption and formation of the alveolar bone, which is required for tooth eruption. Furthermore, root formation with periodontal ligament facilitates continuous tooth eruption. However, the exact mechanism underlying tooth eruption remains unclear. Hence, this review describes the recent research progress on the cellular and molecular mechanisms of tooth eruption.
Dental Sac ; Humans ; Osteoblasts ; Osteoclasts ; Periodontal Ligament ; Tooth ; Tooth Eruption ; physiology ; Tooth Root

BACKGROUND:Ten-eleven translocation (TET) family proteins are recently discovered DNA dioxygenases that convert methylcytosine to hydroxymethyl cytosine, which is essential for regulating cel proliferation and differentiation, but the expression pattern of TET family proteins in human dental pulp cel s is stil unclear. OBJECTIVE:To investigate the expression pattern of TET family proteins during the differentiation of human dental pulp cel s. METHODS:Cel ular distribution and expression of TET family proteins were determined by immunofluorescence in human dental pulp cel s that were cultured and isolated using digestion method. The protein levels of TETs during cel passage (P1-P7) were detected with western blot assay, and their potential changes during odontogenic induction (7 and 14 days) were confirmed using real-time quantitative PCR and western blot analyses at mRNA and protein levels, respectively. RESULTS AND CONCLUSION:Al TETs were expressed in the nucleus and the cytoplasm of human dental pulp cel s During serial cel passage, TET1 protein expression was increased until the 6th passage, TET2 significantly increased at the 2nd and 3rd passages and then decreased (P<0.05), and TET3 showed no statistical y significant change (P>0.05). Both mRNA and protein expression levels of al TETs were elevated during odontogenic induction (P<0.05). These results indicated that TETs may contribute to cel differentiation of human dental pulp cel s.

Objective To investigate the related brain areas and nucleus involved in the inhibition of vagus nerve stimulation (VNS) on epilepsy. Methods Using the kainic acid kindling epilepsy rats model,we observed the distribution of Fos positive neurons in the brain after VNS treatment combined with immunohistochemical method. Results VNS induced a significant increase in Fos immunoreactivity in the bilateral nucleus of solitary tract,the locus coeruleus,parabrachial nucleus,periaqueductal gray of midbrain,lateral habenular nucleus,paraventricular thalamic nucleus,rhomoid thalamic nucleus,paraventricular hypothalamic nucleus.Dense Fos immunoreactive staining was also seen in the central nucleus of amygdala,bed nucleus of stria terminalis,lateral septal nucleus and prepirifiorm cortex.Pretreatment with electric stimulation on cervical vagual nerve stem, c fos expressing of hippocampus formation,cingulate gyrus and frontal,parietal,temporal lobus significantly diminished after KA injection. Conclusion This finding may suggest that VNS activates various brain structure that could be involved in the regulation of seizures.

OBJECTIVE:To explore the approach and starting point for the management of hospital high-alert medications so as to enhance the level of drug safety control.METHODS:By reviewing pertinent literature,the background,concept and categories of high-alert medications as well as the practice and experiences of carrying out management on high-alert medications in our hospital were introduced.RESULTS & CONCLUSIONS:The management on hospital high-alert medications has effectively lowered the risks of it.In view of the frequent occurring drug safety events,it is necessary to implement the corresponding management on high-alert medications in medical institution.It is advisable to establish a corresponding list of high-alert medications as well as a supervising system in medical institutions to improve drug control safety level and risk prevention ability.