1.Discussion of stroke unit management in nursing based upon international standards in neurology department
Qunli XU ; Lijun FENG ; Xiuhua RAO
Chinese Journal of Practical Nursing 2013;29(30):73-75
Objective To discuss the practice of nursing management model of stroke unit based upon the international standards implemented in the neurological unit.Methods The experiences of the stroke unit management were summarized and applied to instruct the clinical practice.Results After the implementation,the average hospitalization days,the proportion of drugs,medical cost,and mortality rate decreased.The satisfactory degree of patients with the stroke unit increased.Conclusions The nursing management model of stroke unit not only improved the quality of nursing service to stroke patients,but also decreases the mortality and disability rate,shortened the length of hospital stay,and enhanced the satisfaction degree of patients and their family members.
2.Clinical efficacy of isotopes radiotherapy with micro-plasma on atrophic scars of facial hemangioma
Liangfu WANG ; Qiuhua RAO ; Wenbing XU
Chinese Journal of Medical Aesthetics and Cosmetology 2015;21(2):95-98
Objective To study the clinical efficacy of treatment of atrophic scars of facial hemangioma after isotopes radiotherapy with micro-plasma.Methods A total of 52 patients with atrophic scars were selected under micro-plasma treatment for 4 times,one time every six weeks.And their efficacies were evaluated by compared with single standard of that before and after the treatment.Results By means of rate of clinical index,the total effective rate of atrophic scars was 82.7%.The degree of improvement was as follows:cured in 53.8%,marked effective in 28.9%,effective in 17.3% and no case ineffective.The total effective rate of hyperpigmentation was 90.3%,including cured in 59.6%,marked effective in 30.8%,effective in 9.6% and no ineffective cases.Conclusions Micro-plasma is an effective therapy for atrophic scars of facial hemangioma after isotopes radiotherapy,which can significantly improve the depression degree of scars,eliminate the hyperpigmentation and improve the color of scars,but it only has adverse reactions of wound pain and postoperative wound erythema.
3.Indirect immunofluorescence and real-time fluorescent PCR for detection of mycoplasma pneumonia in children
Jianrong WANG ; Xu HE ; Fuguang RAO
International Journal of Laboratory Medicine 2015;(18):2633-2634,2637
Objective To compare efficacy of indirect immunofluorescence (IFA) and real‐time fluorescent polymerase chain re‐action(PCR) in detection of mycoplasma pneumonia in children .Methods A total of 137 children clinically diagnosed as Mycoplas‐ma pneumoniae(MP) infection were selected and divided into groups by age ,including <1 years old group(35 cases) ,1- <5 years old group(69 cases) and 5-15 years old group(33 cases) .Blood specimen and throat swabs were collected and detected by using IFA and real‐time fluorescent PCR .At the same time ,all of the selected children were treated with conventional therapy ,according to total effective rate ,positive coincidence rates of the two methods were statistically analysed by age .Results The positive coinci‐dence rates in children with MP infection <1 years old and 1- <5 years old detected by using real‐time fluorescent PCR were high‐er than that detected by using IFA ,while among children 5-15 years old ,the positive coincidence rate was higher detected by using IFA compared with that detected by using real‐time fluorescent PCR ,all had statistically significant differences (P<0 .05) .The o‐verall positive coincidence rates of the two methods were not significantly different(P>0 .05) .Conclusion IFA and real‐time fluo‐rescent PCR both could be used as effective methods for detecting MP ,but there are some differences of detective efficacy between the two methods in each age group .Therefore ,it is suggested that for children under 5 years old real‐time fluorescent PCR might be selected ,for children aged 5 years old and over IFA might be selected ,in order to improve the detection accuracy and provide better guidance to clinical medication .
4.Molecular mechanism and related influence factors of Lin28/Let-7 axsis.
Lihua RAO ; Xiaowen HUANG ; Sheng XU
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(9):663-665
There are 13 members of the Let -7 miRNAs family which is regarded as tumor suppressor gene, locating in nine different chromosome loci. Lin28 acts as negative regulatory factor of miRNA biological recurrence. By selectively blocking the processing synthesis of the Let-7 miRNAs family, Lin28 block the inhibition effect of miRNA of proto-oncogenes and interact with RNA helicase to enhance gene translation at the same time. By not quite clear mechanism, an up-regulation of Let-7 inhibits the expression and function of Lin28. In more and more studies of human tumor, Lin28/Let-7 axsis was proved to be important significance of the tumor's occurrence and development. In this paper, we research briefly the recent progress of the molecular mechanism and related influence factors of ILin28/Let-7 axsis.
Animals
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Humans
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MicroRNAs
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RNA-Binding Proteins
5.Effects of pH and oxygen supply on production of 2,3-butanediol from biodiesel-derived glycerol by Bacillus amyloliquefaciens.
Taowei YANG ; Zhiming RAO ; Xian ZHANG ; Meijuan XU ; Zhenghong XU
Chinese Journal of Biotechnology 2013;29(12):1860-1864
Bacillus amyloliquefaciens B10-127 was used to produce 2,3-butanediol (2,3-BD) from residual glycerol obtained from biodiesel synthesis. Important variables for 2,3-BD fermentation, pH and dissolved oxygen, were studied. When pH was maintained constant, the yield of 2,3-BD was inhibited. The highest 2,3-BD yields were achieved by fermentation without any pH control with an optimized initial pH 6.5. Batch fermentative production of 2,3-BD by B. amyloliquefaciens was investigated using various oxygen supply methods by changing agitation speed. Based on the analysis of three kinetic parameters including specific cell growth rate (micro), specific glucose consumption rate (q(s)) and specific 2,3-BD formation rate (q(p)), a three-stage agitation speed control strategy was proposed, aimed at achieving high concentration, high yield and high productivity of 2,3-BD. Maximum concentration of 2,3-BD reached 38.1 g/L, with the productivity of 1.06 g/(L x h), which were 14.8% and 63.1% over the best results from constant agitation speeds. In a pulse fed-batch fermentation, 2,3-BD concentration and productivity were significantly improved to 71.2 g/L and 0.99 g/(L x h), respectively. To our knowledge, these results were the highest for 2,3-BD production from biodiesel-derived glycerol.
Bacillus
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classification
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metabolism
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Biofuels
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analysis
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Bioreactors
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Butylene Glycols
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metabolism
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Fermentation
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Glycerol
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metabolism
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Hydrogen-Ion Concentration
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Industrial Microbiology
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Oxygen
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analysis
6.The curative effect of livostin spray on treating allergic rhinitis of children
Shufen WANG ; Zhongqiang XU ; Zhinan WANG ; Kaicheng RAO ; Ruiming XU
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2001;(4):171-172
Objective:To observe the effect of Livostin spray on children's allergic rhinitis and to search the mechanism of treating allergic rhinitis. Method: 113 patients were treated with Livostin spray (Livostin group) or normal saline spray (control group). Result:The total efficiency of Livostin group in treating allergic rhinitis is above 95.1% and that of the control group is 25.0%. Initial time of starting effect of Livostin (72.1%) is in 1 minute, and that of the control group (mostly 23.1%) is in 3 minutes. The keeping curativeeffect time of Livostin spray is mostly (72.1%) above 5 hours and that of the control group is mostly (30.8%) in 3 hours. After 2 weeks,the eosinophilic granulocyte number in nose's secretion of Livostin group is obviously reduced (P<0.05). Conclusion: Livostin is better than control group in relieving symptoms, keeping curative effect and safety,so Livostin is one kind of effective drug in treating children's allergic rhinitis.
7.Clinical study of improved decortication in treatment of tuberculous empyema
Dong WANG ; Zongxiang XU ; Hanjiang ZHANG ; Fei RAO
Chinese Journal of Postgraduates of Medicine 2012;35(9):26-28
ObjectiveTo study the clinical effect of improved decortication in treatment of tuberculous empyema as well as the safety of operation.MethodsEighty-two cases who diagnosed as tuberculous empyema by pathology and operation from January 2007 to September 2010 were selected including improved decortication 44 cases,total empyema decortication 28 cases,thoracoplasty 10 cases.The operation time,peri-operative bleeding,postoperative complication and lung function recovery after 6 months operation were followed-up.ResultsAll the patients were in good recovery and there was no death in the operation for 1 year.The peri-operative bleeding in improved decortication was less than that in total empyema decortication and thoracoplasty [(56.23 ± 15.56) ml vs. (78.65 ± 23.14) and (66.92 ± 19.83) ml],and there was significant difference among them(P< 0.01 ).There was no postoperative complication in improved decortication,but 2 cases (7.1%,2/28) of partial lung recruitment maneuvers in total empyema decortication,1 case ( 10.0%,1/10) of extensive staxis with selective surgery in thoracoplasty.The proportion of forced vital capacity (FVC),forced expiratory volume in one second (FEV1),peak expiratory flow (PEF) turning to normal after 6 months operation in improved decortication [95.5%(42/44),93.2%(41/44),97.7%(43/44)]were higher than those in total empyema decortication[ 75.0% (21/28 ),78.6% (22/28),85.7% (24/28) ]and thoracoplasty [ 80.0% (8/10),90.0% (9/10),80.0% ( 8/10) ],and there was significant difference among them (P <0.01).There was no significant difference in the operation time among them(P >0.05).ConclusionImproved decortication has remarkable superiority in curing tuberculous empyema especially it is small in traumatic with less blood loss,fewer postoperative complications,lung function recovery rapidly after operation.
8.Preparation of anti-HER2 monoclonal antibody-paclitaxel immunoconjugate and its biological evaluation.
Dong, LIU ; Yanjiao, XU ; Zichao, RAO ; Zhaocong, CHEN
Journal of Huazhong University of Science and Technology (Medical Sciences) 2011;31(6):735-40
Anti-HER2 monoclonal antibody (Sc7301)-paclitaxel (TAX) immunoconjugate was prepared and its specific binding to tumor cells was investigated in this study. Sc7301 was conjugated to TAX by the active ester method and then the TAX-Sc7301 immunoconjugate was obtained. After purification and labeling by Cyano-fluorescein isothiocyanate (FITC), the specific binding of TAX-Sc7301 to HER2-positive tumor cells (SKOV3) and HER2-negative tumor cells (HepG2) was evaluated respectively. TAX-Sc7301 (20 nmol/L) showed distinct specific binding to SKOV3 cells rather than HepG2 cells. And the uptake of the immunoconjugate by SKOV3 cells was increased with the TAX-Sc7301 concentration (3-48 nmol/L) and the incubation time (P<0.05). It was concluded that the TAX-Sc7301 immunoconjugate is potentially applicable as a targeted agent against HER2-positive tumor cells.
9.RECIPROCAL PATHWAYS BETWEEN MEDULLARY VISCERAL ZONE AND HYPOTHALAMIC PARAVENTRICULAR NUCLEUS OR SUPRAOPTIC NUCLEUS INVOLVED IN HYPEROSMOTIC REGULATION
Zhijun YANG ; Zhiren RAO ; Ruxiang XU ; Al ET ;
Medical Journal of Chinese People's Liberation Army 2001;0(11):-
Objective To elucidate the existence of reciprocal pathways between medullary visceral zone (MVZ) and hypothalamic paraventricular nucleus (PVN) or supraoptic nucleus(SON) following hyperosmotic stimulation. Methods Hyperosmosis was produced in rats by drinking 3% sodium chloride solution. The distributions and expressions of HRP retrogradely labeled neurons, Fos, tyrosine hydroxylase (TH) or vasopressin (VP) positive neuron and glial fibrillary acidic protein (GFAP) positive astrocytes(AST) in MVZ, SON and PVN were observed by quadruplicate labeling methods of WGA HRP retrograde tracing combined with anti Fos, TH (or VP) and GFAP immunohistochemical technique. Results Fos positive neurons within the MVZ, PVN and SON were increased markedly. There was also a marlced increase in GFAP positive structures in the brain, and their distribution pattern was fundamentally similar or analogous to Fos positive neurons in the above mentioned areas. The augmented GFAP structures manifested as hypertrophic cell bodies, with thicker and longer processes. Quadruplicate immunohistochemical staining showed that neurons were surrounded by many AST to form neuron astrocytic complex (N ASC). Conclusion The neurons and AST, as a functional unif in the form of N ASC, might actively participate in modulating osmotic pressure. There was reciprocal osmoregulation pathways between the MVZ and SON or PVN in the brain
10.EXPRESSION OF FOS-LIKE PROTEIN IN BRAIN FOLLOWING VAGUS NERVE STIMULATION IN EPILEPTIC RATS
Guohong TIAN ; Li DUAN ; Zhiren RAO ; Yuangui HUANG ; Yan XU ;
Acta Anatomica Sinica 1957;0(04):-
Objective To investigate the related brain areas and nucleus involved in the inhibition of vagus nerve stimulation (VNS) on epilepsy. Methods Using the kainic acid kindling epilepsy rats model,we observed the distribution of Fos positive neurons in the brain after VNS treatment combined with immunohistochemical method. Results VNS induced a significant increase in Fos immunoreactivity in the bilateral nucleus of solitary tract,the locus coeruleus,parabrachial nucleus,periaqueductal gray of midbrain,lateral habenular nucleus,paraventricular thalamic nucleus,rhomoid thalamic nucleus,paraventricular hypothalamic nucleus.Dense Fos immunoreactive staining was also seen in the central nucleus of amygdala,bed nucleus of stria terminalis,lateral septal nucleus and prepirifiorm cortex.Pretreatment with electric stimulation on cervical vagual nerve stem, c fos expressing of hippocampus formation,cingulate gyrus and frontal,parietal,temporal lobus significantly diminished after KA injection. Conclusion This finding may suggest that VNS activates various brain structure that could be involved in the regulation of seizures.