3.Effect of Guizhi Decoction on cardiac muscular collagen reconstruction in spontaneous diabetes rats
Xiao LI ; Ping JIANG ; Yunsheng XU ; Yong ZHANG ; Yuehua JIANG
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(08):-
Objective:This study was to study effect of keeping ying and weiqi in balance on cardiac muscular collagen Ⅰ,collagen Ⅲ,angiotensinⅡ in spontaneous diabetes rats,and provide new thoughts for prevention and treatment for diabetic cardiomyopathy.Methods:40 spontaneous diabetes rats were divided into four groups at random:spontaneous diabetes control group,diformin group,Guizhi Decoction group and Huanglian Jiedu Decoction group.Except control group,each group was given intragastric administration for 12weeks.Blood glucose,collagenⅠ,collagen Ⅲ,collagenⅠ/collagen Ⅲ,angiotensin Ⅱ were determined.Euzymelinked immunosorbent assay and radio-immuniy method were used.Results:Blood glucose in Huanglian Jiedu Decoction group and diformin group decreased;Compared respectively with diformin group and control group,collagenⅠdecreased obviously and collagen Ⅲ increased signifi cantly in Guizhi Decoction group,and the collagenⅠ/collagen Ⅲ decreased.Compared with diformin group and control group,cardiac muscular homogenate AT-Ⅱ contents decreased in both of Guizhi Decoction group and Huanglian Jiedu Decoction group.Conclusion:Guizhi Decoction can decrease the content of collagen Ⅰ,AT-Ⅱ and collagenⅠ/collagen Ⅲin cardiac muscular,and increase content of collagen Ⅲ,then prevent and treat cardiac muscular collagen reconstruction and myocardial fibrosis.Huanglian Jiedu Decoction can decrease the content of AT-Ⅱ,and decrease collagenⅠ/ collagen Ⅲ,collagenⅠ slightly,and can improve cardiac muscular collagen reconstruction.Keeping ying and weiqi in balance is a new thought for prevention and treatment for diabetic cardiomyopathy.
5.Effect of Continuous Blood Purification on Inflammatory State and Immune Response in Patients with Multiple Injury and Sepsis
Lin XU ; Congbing JIANG ; Wei CHENG ; Ping XU ; Jianjun ZHANG
Progress in Modern Biomedicine 2017;17(24):4691-4694
Objective:To study the effect of continuous blood purification on inflammatory state and immune response in patients with multiple injury and sepsis.Methods:88 patients with multiple injury and sepsis who were treated in our hospital from January 2015 to May 2016 were selected as the research object,they were divided into the control group (n=44) and observation group (n=44) according to random number table method.The control group was given conventional treatment,and the observation group was given continuous blood purification treatment.The acute physiology and chronic health conditions Ⅱ (APACHE Ⅱ)score was observed in two groups Ⅱ at 3 d after treatment.The serum content of Interleukin-2(IL-2),Interleukin-4(IL-4),Interleukin-10(IL-10) and interferon-γ (IFN-γ) were detected by ELISA method.The levels of CD3 +,CD4 +,CD8 + and NK cell were analyzed by flow cytometry.The urea nitrogen (BUN),serum creatinine (Scr),prothrombin time (PT),thrombin time (TT),activated partial blood coagulation time (APTT) were tested by automatic biochemical analyzer.Results:The APACHE Ⅱ score,BUN and Scr levels in observation group were lower than the control group (P<0.05).The content ofIL-2,IL-4,IL-10 and IFN-γ in observation group were lower than the control group (P<0.05).The levels of CD3+,CD4+,CD8+ and NK in observation group were higher than the control group (P<0.05).The levels ofPT,TT and APTT in observation group were lower than the control group (P<0.05).Conclusion:Continuous blood purification in patients with multiple injury and sepsis had better clinical curative effect,can reduce inflammation,improve immune function and the function of blood coagulation.
6.A MICROWAVE-BASED METHOD FOR GENOMIC DNA EXTRACTION FROM ACTINOMYCETES
Ping XU ; Wenjun LI ; Lihua XU ; Chenglin JIANG ;
Microbiology 1992;0(04):-
The original methods on DNA extraction are not only complex and expens ive, but although invalid with actinomycetes under extreme environments Genomi c DNA can be extracted directly from biomass picked up from solid medium by micro wave thermal shock The extracted DNA is suitable for PCR The method is effec ti ve, easy and fast, and can be used in actinomycete systematics and identificatio n rapidly
7.Technique for hepatic veins in liver surgery.
Yi-tao DING ; Qing-xiang XU ; Chun-ping JIANG
Chinese Journal of Surgery 2012;50(6):488-490
Hepatectomy
;
methods
;
Hepatic Veins
;
surgery
;
Humans
;
Liver
;
surgery
9.The research and practice of innovation and development in nursing management in military hospital
Ping SHEN ; Xueming YI ; Qixia JIANG ; Wei XU
Journal of Medical Postgraduates 2003;0(08):-
Objective: To explore the way of innovation and development of the pattern and means in nursing management in military hospital.Methods:Through various innovative practice from the following aspects,such as nursing service philosophy and measures for soldiers,the methods of nursing management and the elite-cultivating,nursing security management,which demonstrates the availability of innovation and development of the pattern and means in nursing management in military hospital.Results:The satisfactory rate of nursing service reached up to 99% in the examination of serving for soldiers of the whole army.The instruments which are manufactured for battle-scarred wounds and injuries use the flat of the Wound Nursing Centre,have reached the stage of clinical trial,we have completed five items of serial nursing research.The control rate of nursing quality of the whole hospital increased from 93.12% in 2003 to 98.9% in 2006,the satisfactory ratio of serious in-patient increased from 90.2% in 2003 to 96.7% in 2005.The ratio of health education achieves 100%.The satisfactory ratio of in-patient was 98.4% in September 2005,compared with 89.3% in February 2004.Conclusion:Only innovation can make nursing management develop and improve in military hospital.
10.Function Study of the Nano-Molecular PAMAM as miR Carrier Targeting Gastric Adenocarcinoma
Wei SONG ; Zhongmin JIANG ; Ping XU ; Yinghong LIANG ; Xiaozhi LIU
Tianjin Medical Journal 2013;(12):1147-1149
Objective To study the function of the nano-molecular polyamide-amine (PAMAM) as microRNA(miR) carrier targeting gastric adenocarcinoma, and the foundation of developing an efficient delivery of small molecule drugs tar-geting gastric cancer thereof. Methods The folic acid (FA)/PAMAM comoles compound was prepared by dialysis method. After transfection of miR-7 or liposomes into SGC-7901 cell line, fluorescence microscope was used to detect the gene trans-fect efficiency. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the miR-7 level. The immu-nocytochemistry assay was used to test the protein expressions of epidermal growth factor receptor (EGFR), protein kinase B (PKB) and proliferating cell nuclearantigen (PCNA). The transwell system was utilized to explore the migration ability of tu-mor cells. Results Compared with liposme, FA/PAMAN complex compound can significantly improve the level of miR-7 in SGC-7901 cells,reduce the protein levels of EGFR, PKB and PCNA in SGC-7901 cells, and also reduce the percentage of cancer cell migration (P<0.05). Conclusion PAMAM can effectively transfect miR into gastric cancer cells, which is expected to become an efficient delivery of small molecule drugs.