Objective To compare the serum pepsinogen (PG)in different altitude population.Methods By testing serum PG of people from different altitudes,detected the concentration of serum PG.Results The serum PGI,serum PGII and PGR were respectively 136.89±22.64 μg/L,10.05 ± 5.14 μg/L and 13.07 ± 7.41 in 1 000 m group,and they were 30.87 ± 48.66 μg/L,11.55±2.78 μg/L and 12.89±4.76 in 1 300 m group.There were significant difference in PGI and PGII(P <0.05)except PGR (P >0.05)between the two groups.The corresponding indices in 1500m group (126.64±29.84 μg/L, 12.18±5.99 μg/L and 12.2±14.84,respectively),the serum PGI was decreased obviously compared with those groups (P<0.05),the serum PGII was increased obviously compared with those groups (P < 0.05),No significant difference was found when compared with those groups (P >0.05).The serum PGI was negative correlated with higher altitudes,the ser-um PGII was positively correlated with higher altitudes.Conclusion The study found that the serum PGI content would re-duce with the increase of altitude,and on the contrary the serum PGII would rise with the increase of altitude.The main rea-son that the serum PG change may be influenced by oxygen partial pressure.

Objective To explore the differences of H .pylori infection between residents in different humidity environment. Methods 137 persons from Sanjiang of Wenchuan and 320 persons from Chengdu were examined.Blood samples were ob-tained for serological analysis by dot immunochromatographic assay.Results The infection rate of H .pylori in Wenchuan of Sanjiang residents was 26.28%,and the Chengdu rate was 16.88% (P <0.025),with the significant difference among which,and removed the effection of nation constitution.Conclusion The differences of H .pylori infection rate between res-idents in different humidity environment were existent.High humidity environment maybe one of factors that lead to high H.pylori infection rate.

Although there is emerging evidence that BJ46a can function as potent inhibitor of the SVMPs proteolytic activities,its anticancer effect on invasion and metastasis has not yet been evaluated.Inhibition effect of BJ46a on experimental pulmonary metastasis in mice inoculated with B16 melanoma cells at the protein level was investigated. First,BJ46a was produced in baculovirus expression system. SDS-PAGE and Western blot analysis confirmed that BJ46a recombinant protein was produced by Sf9 cells infected with high-titer viral stock. Then,recombinant fusion protein was purified by ProBondTM at the point of maximal expression. B16 cells pre-treated with recombinant BJ46a injected into C57BL/6 mice via the tail lateral vein to form experimental pulmonary metastasis model. The numbers of metastatic lesions in C57BL/6 mice changed dramatically:BJ46a different concentrations of recombinant protein group were 1.1 ? 0.83,0.9 ? 0.7,significantly lower than the control group (6.3?3.00,P

Acute Disease ; Humans ; Male ; Middle Aged ; Thrombocytopenia ; chemically induced ; Tyrosine ; adverse effects ; analogs & derivatives

OBJECTIVE To analyze the cause of Acinetobacter baumannii resistance to ?-lactam and the aminoglycoside-modifying antibacterials. METHODS Three-dimensional test was used to analyze and classify the ?-lactamases. Proper primers was used to do PCR and determined by sequencing. RESULTS A. baumannii clinical isolate harbored blaOXA2-23,blaTEM and blaADC genes and aac(3)-Ⅰ,aac(6')-Ⅰb and ant(3″)-Ⅰ aminoglycoside-modifying enzyme genes. CONCLUSIONS An A. baumannii strain which carries TEM,OXA-23,ADC ?-lactams and aac(3)-Ⅰ,aac(6')-Ⅰb,ant(3″)-Ⅰ aminoglycoside-modifying enzyme genes is detected.

The accuracy and repeatability of computer aided cervical vertebra landmarking (CACVL) were investigated in cephalogram. 120 adolescents (60 boys, 60 girls) aged from 9.1 to 17.2 years old were randomly selected. Twenty-seven landmarks from the second to fifth cervical vertebrae on the lateral cephalogram were identified. In this study, the system of CACVL was developed and used to identify and calculate the landmarks by fast marching method and parabolic curve fitting. The accuracy and repeatability in CACVL group were compared with those in two manual landmarking groups [orthodontic experts (OE) group and orthodontic novices (ON) group]. The results showed that, as for the accuracy, there was no significant difference between CACVL group and OE group no matter in x-axis or y-axis (P>0.05), but there was significant difference between CACVL group and ON group, as well as OE group and ON group in both axes (P<0.05). As for the repeatability, CACVL group was more reliable than OE group and ON group in both axes. It is concluded that CACVL has the same or higher accuracy, better repeatability and less workload than manual landmarking methods. It's reliable for cervical parameters identification on the lateral cephalogram and cervical vertebral maturation prediction in orthodontic practice and research.

BACKGROUND: Mesenchymal stem cells are few in human bone marrow, and their number will decrease with aging or body weakening, so a large amount of amplification is necessary. However, the biological characteristics of human mesenchymal stem cells of each passage remain poorly understood.OBJECTIVE: To analyze and compare the biological characteristics of each passage of bone marrow-derived mesenchymal stem cells (MSCs) so as to provide a basis for clinical demands of tissue engineering.DESIGN,TIME AND SETTING: Cytological observation in vitro. The experiment was performed at the Department of Orthopedics and Central Laboratory, Dongzhimen Hospital, Beijing University of Chinese Medicine from March to October 2008.MATERIALS: From bone marrow of patients with non-hematopoietic disease, MSCs were provided by Department of Orthopedics, Dongzhimen Hospital, Beijing University of Chinese Medicine.METHODS: Bone marrow was collected form posterior superior iliac spine of patient, MSCs were isolated and cultured by Percoll method. When the cells were confluent at 90%, they were trypsinized and observed by inverted miscroscopy. The second passage of cells were collected for index detection.MAIN OUTCOME MEASURES: Cell morphological characteristics and immunophenotype; cell activity was detected by MTT; cell division and apoptosis in the proportion of necrosis were analyzed by flow cytometry analysis.RESULTS: The passaged MSCs exhibited uniform appearance in fusiform shape, and their growth was slowed down after 9 passages, exhibiting cytoplasm vacuolization and body enlarging. The second passage of MSCs was positive for CD44, CD106,and CD105, but negative for CD34 and CD45. MTT values peaked at passage 9, and gradually decreased since passage 10. At passage 11, the number of MSCs at division stage was increased, but from the sixth passage, the number of apoptotic cells increased significantly, reaching more than 60% at passage 8.CONCLUSION: According to biological characteristics analysis of MSCs at each passage, the third to the sixth passage cells are recommend for clinical therapy.

Objective To analyze the clinical significance of patients self -intervention on self -efficacy and lipid levels in patients with cerebral infarction.Methods 300 patients with cerebral infarction in our hospital were selected from May 2014 to May 2015,and they were randomly divided into two groups by digital table method:the observation group(n =1 50)and the control group(n =1 50),the two groups of patients had no significant differences in the general data.The observation group treated according to the conventional rehabilitation treatments for rehabilitation nursing,in view of the self -management group.After three months,the two groups of patients'self -efficacy and lipid levels were recorded before and after the intervention and the patients'self management abilities were measured. Comparisons between before and after the intervention of the indicators and between the different groups were analyzed,and the relationship of self -management ability and lipid levels of patients was analyzed by Pearson correlation analysis.Results Before the intervention,there was no difference between the two groups in the self -efficacy scale(P >0.05).After the treatment,the self -efficacy scale of the observation group was (29.85 ±5.34), which of the control group was (22.74 ±4.73),the difference between the two groups was statistically significant(t =1 2.207,P <0.001 ).For the lipid levels after treatment,the TC,TG,LDL -C,HDL -C in the observation group were (4.06 ±0.58)mmol/L,(1 .26 ±0.56)mmol/L,(2.27 ±0.49)mmol/L,(1 .42 ±0.54)mmol/L,which in the control group were (4.86 ±1 .05)mmol/L,(1 .54 ±0.48)mmol/L,(3.1 5 ±0.29)mmol/L,(1 .23 ±0.37)mmol/L, the differences between the two groups were statistically significant (t =8.1 68,4.649,1 8.929,3.555,all P <0.001 ).The correlation analysis of self -management ability and the blood lipid improvement of the two groups showed that the observation group presented positive correlation.The patients'self -management ability was stronger, its lipid improve ability was better.Conclusion From the self -efficacy and the blood lipid level analysis,cerebral infarction patients'self -intervention can promote physical and mental health and improve the symptoms of cerebral infarction.In summary,it is helpful for recovery and has positive clinical effect on cerebral infarction.

[Objective]To investigate the prognostic value of 18F-FDG PET/CT with different metabolic parameters in newly diagnosed limited-disease(LD)small cell lung cancer(SCLC).[Methods]Retrospective analysis was carried out in the patients between June 2005 to December 2016 in our hospital confirmed of LD SCLC by pathology or cytology and comprehensive imaging. Fifty-four patients were recruited. Record the general characteristics of patients,pre-treatment KPS score,smoking status,weight loss,serum LDH,NSE,OS,PFS. All lesions(primary lesions + metastases)were sketched out within one VOI,and the SUVmax, SUVmean and SUVpeak in the VOI were automatically measured and recorded. The automatic measurement was performed by the fixed threshold method. The thresholds of tumor of MTV and TLG were 40% and 50% of SUVmax. The TLG and MTV were identified as TLG40%,TLG50%,MTV40% and MTV50% respectively. Kaplan-Meier method was used for survival analysis. All the prognostic factors were analyzed by Cox model.[Result]The median SUVmax was 13.92(2.61~43.28),the median of SUVmean was 8.31(1.71~26.85) and the median of SUVpeak was 10.51(1.49 ~ 27.48). The median of TLG40% was 340.22(16.58 ~ 2827.26),the median of TLG50%was 215.645(1.70 ~ 2270.36),the median of MTV40% was 36.71(1.15 ~ 259.47 cm3),the median of MTV50% was 19.65(0.93 ~1900.00)cm3. Univariate and multivariate analysis of metabolic index and prognosis showed that TLG50% was the prognostic factor of OS(P = 0.013),but not of PFS(P > 0.05). The SUVmax,SUVmean and SUVpeak were not the prognostic factors of OS and PFS(P >0.05).[Conclusion]The volume metabolic parameters TLG50%was the independent prognostic factor of the overall survival time of the LD SCLC. The volume metabolic parameters (TLG and MTV) of 18F-FDG PET/CT were related to the prognosis of SCLC ,which could provide the basis for individual chemotherapy.

Objective:To assess the prevalence of HIV primary drug resistance and drug resistance gene mutations among men who have sex with men (MSM).Methods:We searched eight electronic databases (CNKI,VIP,CBM,Wanfang Database,PubMed,Web of Knowledge,Springer,Medline) for the studies of HIV drug resistance relevant to MSM.Drug resistance and drug resistance mutations data were pooled and analyzed according to statistical test of homogeneity.Subgroups were further divided according to sample size,location,race,quality rating score,sampling time.Results:Forty-three studies were included in this Meta-analysis.The pooled rate of total to protease inhibitor (PI),nucleoside reverse transcriptase inhibitor(NRTI) or non-nucleoside reverse transcriptase inhibitor (NNRTI) were 10.21％ (95％ CI 8.65％ to12.03％),2.98％ (95％ CI 2.25％ to 3.93％),4.05％ (95％ CI 3.14％ to 5.21％),4.42％ (95％ CI 3.31％ to 5.88％),respectively.The pooled rates of PI major mutation,PI secondary mutations,NRTI mutations and NNRTI mutations were 0.55％ (95％ CI 0.38％ to 0.80％),1.31％ (95％ CI 0.98％ to 1.75％),0.85％ (95％ CI 0.51％ to 1.40％),1.19％ (95％ CI 0.70％ to 2.01％),0.79％ (95％ CI 0.55％ to 1.13％),1.73％ (95％ CI 1.21％ to 2.46％),0.86％ (95％ CI 0.61％ to 1.21％),2.24％ (95％ CI 1.77％ to 2.83％),respectively.Sample size,region,and race were heterogeneous sources;the rate of resistance mutations and gene mutation rate were different in different subgroups.Conclusion:The prevalence of primary drug resistance among MSM was high in Americas and Europe,and it was gradually increased in Asia.We should pay attention to the high incidence of PI secondary mutations.