In condition of direct vision on fundus of eye, microperimeter can quantitatively detect the retinal light sensitivity of macular area, and record real time tracking of the eye, automatically analyze the location and stability of fixation. Microperimeter matches fundus image with micro cyclogram point-to-point, thus it achieves the combination of visual function and structure. The characteristics of microperimeter have good relations with subjective vision, so it can be a new method for the accurate vision evaluation and has application potential to assess the visual function in legal medicine. In this ar-ticle, we summarize the principle, method and parameters of microperimeter. Also, the applications of microperimeter in vision assessment are focused in order to provide a reference for the assessment of visual function in the legal medicine.

AIM: To study the effects of l-menthol and its binary systems on percutaneous penetration and deposit effects of 5-fluorouracil (5-FU). METHODS: Percutaneous absorption experiment was performed on the apparatus of isolated skin, penetrated amounts of different times of 5-FU were determined, the cumulate penetrated amounts and permeation rates and increased permeation times were calculated, and the deposit effect of 5-FU was investigated. RESULTS: L-menthol of different concentrations could significantly enhance the percutaneous penetration of 5-FU, its increased permeation times were 1.25 and 1.45 and 1.37 times respectively,and the action of 5-FU with 2% l-menthol was the strongest. 5-FU with 2% and 4% l-menthol had a marked deposit effect. When some kinds of enhancers were used alone or combined with l-menthol, the percutaneous penetration of 5-FU had a significantly increase. CONCLUSION: L-menthol and its binary systems can markedly promote the percutaneous penetration and the deposit effect of 5-FU.

AIM To study the effect l-menthol pretreated nasal cavity on insulin pharmacological bioavailability(PBA). METHODS To set up diabetic rats model,nasal cavities were pretreated with l-menthol for two hours,insulin was dripped, blood glucose concentrations of different time were determined,and the area over the curve (AOC) and pharmacological bioavailability(PBA) were calculated. RESULTS After rats nasal cavity was pretreated with different concentrations l-menthol, nasal cavity absorption of insulin markedly increased between 2 h and 3 h,and PBA of insulin nasal cavity absorption increased respectively 5.77,7.00 and 3.12 times than control group. CONCLUSION l-Menthol promotes the nasal cavity absorption of insulin.

In condition of direct vision on hindus of eye, microperimeter can quantitatively detect the retinal light sensitivity of macular area, and record real time tracking of the eye, automatically analyze the location and stability of fixation. Microperimeter matches hindus image with micro cyclogram point-to-point, thus it achieves the combination of visual function and structure. The characteristics of microperimeter have good relations with subjective vision, so it can be a new method for the accurate vision evaluation and has application potential to assess the visual function in legal medicine. In this article, we summarize the principle, method and parameters of microperimeter. Also, the applications of microperimeter in vision assessment are focused in order to provide a reference for the assessment of visual function in the legal medicine.
Forensic Medicine/legislation & jurisprudence* ; Humans ; Retina ; Vision, Ocular ; Visual Acuity ; Visual Field Tests

Chronic Disease ; Humans ; Sinusitis ; diagnosis ; therapy

Objective:To investigate the effect of synthetic borneol on penetration mechanism of ligustrazine hydrochloride. Methods: Permeation tests in vitro through rabbit skin in two compartment diffusion cells were performed to study the effect of synthetic borneol on transdermal absorption of ligustrazine hydrochloride by changing concentrations of synthetic borneol and rabbit skin condition. Results: Ligustrazine hydrochloride permeation coefficient increased linearly with incerease of concentrations of synthetic borneol, while those through strippde stratum corneum skin were not varied. Synthetic borneol enhanced the deposit functions of whole skin and stripped stratum corneum skin. Conclusions: Synthetic borneol could enhance the percutaneous absorption of ligustrazine hydrochloride, mainly through the stratum corneum, and increase the deposit function.

AIM: To investigate the nasal absorption of ligustrazine and promotion action of synthetic borneol. METHODS: In situ nasal recirculation method was used to study the dose effect relationship of nasal absorption of ligustrazine and the effect of synthetic borneol on nasal absorption of ligustrazine in rats. RESULTS: When the concentrations of ligustrazine were 0.25 , 0.75 ,and 1.25 g?L -1 in circulation liquid, respectively, the corresponding values of the absorption rate constant k were 0.0195 , 0.0227 , and 0.0241 min -1 . However, when the concentrations were 2.5 and 5.0 g?L -1 in circulation liquid, the absorption rate constant k lowered with the concentration being added. CONCLUSION: Ligustrazine can be absorbed through the nasal mucous and synthetic borneol can promote the absorption.

Animals ; Bridged-Ring Compounds ; poisoning ; Disease Models, Animal ; Female ; Male ; Mice ; Sodium Oxybate ; therapeutic use ; Unithiol ; therapeutic use

Obiective To investigate the change of the activity of proliferation in cultivated M?ller cells treated by advanced glycation endoproducts (AGEs), and the effect of these changes on expression of occludin in bovine retinal vascular endothelial cells (BREC). Methods The cultivated M?ller cells were devided into normal growth group and cultured with AGEs group. The cultured BREC were devided into 4 groups:group 1, without any medium; group 2, with normal growth M?ller cell medium (MCM); group 3,MCM treated by AGEs; group 4, without cell as the control. Enzyme-linked immuno sorbent assay was used to detect the content of occludin in the medium in the 4 groups. Results The content of expression of occludin was the most in group 2, less in group 1, and the least in group 3. Conclusion AGEs may promote the abnormal proliferation of M?ller cells and inhibit the expression of occludin in BREC.