Nonglomerular synapses in the neuropil of the substantia gelatinosa of adult ratspinal trigeminal nucleus were subjected to electron microscopic examination. Nonglomerular synapses in the substantia gelatinosa were associated with four main types of axonal terminals. On the basis of vesicle shapes, these were classified as round vesicle, elongate vesicle, pleomorphie vesicle, and dense cored vesicle terminals. The category of round vesicle terminals could be subdivided into large round vesicle terminals and small round vesicle terminals according to their different size of synaptic vesicles. Nonglomerular synapses included four types, i. e. axodendritic, axo-axonic, dendro-dendritic and dendro-axonic synapses. Of these, the majority of synapses were axodendritie. The functional significance of the classification of these synapses and their axonal terminals had been discussed.

Large dense core vesicle in the trigeminal subnucleus caudalis of the rat has been observed electron microscopically. It is fixed in glutaraldehyde-osmium tetroxide following removal of the skin of the vibrissae areas. The findings of the present study are: 1.Morphological evidence for exocytosis of large dense core vesicle from non-synaptic sites of the axonal terminal has been presented.2. The large coated vesicles equipped with central densities derived from the invagination of the plasma membrane. These observations suggest that membrane recycling occur at location in the terminal via these coated vesicles.3.Some large dense core vesicles may also form the tubular structures which may represent smooth endoplasmic reticulum containing dense material. This study supports the hypothesis that release of the transmitter of the great dense core vesicles occur at nonsynaptic sites by exocytosis.

AIM To evaluate the value of arsenic trioxide (ATO) in the treatment of systemic lupus erythematosus and to investigate its mechanism. METHODS① Thirty four BXSB lupus mice were averagely and randomly divided into ATO treated group and control group. The mice of ATO treated group were given (ip) ATO 0.4 mg·kg-1 every other day until d 105 and the observation was ended on d 210. The survival rate of mice was recorded, and the levels of serum IgG and anti-dsDNA antibody were measured with enzyme-linked immunosorbent assay. ② Other 20 BXSB lupus mice were also divided into 2 groups and treated as above and sacrificed on d 90. The spleen and kidneys of each mouse were removed and total RNA was extracted. The levels of interferon-γ (IFN-γ) mRNA in renal and spleen tissues were measured by using reverse transcription polymerase chain reaction. RESULTSUp to d 210, 8 mice died in ATO treated group and 13 died in control group. On d 90 and d 105, the average levels of serum anti-dsDNA antibody (A450 nm) were (0.335±0.011) and (0.223±0.017) in ATO treated group, and (0.688±0.016) and (0.683 ±0.014) in control group. On d 90, the expressions of IFN-γ mRNA in spleen and renal tissues of ATO treated group were significantly lower than that of control group. On d 105, the serum level of IgG was much lower in ATO treated group than that in control group, which were (4.9±1.3) and (6.9±1.0)g·L-1, respectively. CONCLUSION ATO elevates the survival rate, lowers the serum levels of IgG and anti-dsDNA antibody, and depresses the expression of IFN-γ mRNA in spleen and kidney tissues of BXSB mice.

The occurrence and development of malignant glioma are closely related to abnormal overexpression and activation of receptor tyrosine kinase (RTK) signal transduction pathways.Targeted therapeutic drugs such as RTK inhibitors,RTK downstream signaling pathway inhibitors and multi-target inhibitors can targeting treat malignant glioma at molecular level,some of which have been investigated in clinical trials and achieved good therapeutic effects.

The substantia gelatinosa of subnucleus caudalis of the trigeminal nerve of the rat shows an intense extralysosomal acid phosphatase activity which disappears after the removal of it's skin of the facial regions.Different regions of the facial skin of 69 rats were excised and the projections of the cutaneous nerves were traced by studying the extralysosomal acid phosphatase activity with the modified Gomori's methods.A somatotopical localization was found within the substantia gelationosa. It agreed with what has been found in earlier studies where other techniques have been used. The findings showed that the fibers of the cutaneous areas along the dorsoventral axis of the body were found in a reverse order in the receptive fields in the substantia gelatinosa. The vertex and fore-head lay in the ventral, while skin of the mandibular areas in the dorsal region, and the skin of the facial areas lay successively from ventral to dorsal in following order: vertex and forehead eye region, dorsal vibrissae, ventral vibrissae-upper lip and finally lower lip-mandibulare region, in the receptive fields of the substantia gelatinosa.The cutaneous facial regions were disproportionately represented in substantia gelatinosa, for example, the field receiving projections from the vibrissae areas or fields were as large as 4mm~2 in the most medial portion of the ventral vibrissae-upperlip which are disproportionately large while the fields receiving projections from the forehead and mandibular cutaneous regions were disproportionately small. The provides a possible explanation for the specific function of analgesia of the acupuncture point Renzhong.

The peripheral nerves supplying the acupuncture points of the facial regionwere sectioned from 63 rats and their central terminations were traced by the loss ofthe acid phosphatase(ACP)activity in the substantia gelatinosa(SG)with themodified Gomori's method.1)The supraorbital nerve was cut,the loss of the ACP was confined to thelateral aspect of the substantia gelatinosa in spinal segments C_1.2)The infraorbi-tal nerve was transected,the ACP activity was absent from the central and lateralsectors of the SG in the medulla oblongata.3)The mental nerve was divided,theACP activity was absent from the most medial sector of SG in the medulla oblon-gata and C_1.4)The auriculotemporal nerve was severed,the activity of the enzymewas absent mainly in the inner medial sector of SG in the C_1 and C_2.5)Thefacial nerve was divided,the area deprived of enzyme activity is mainly found inthe central medial sector of SG in C_2.6)The vagal nerve was divided,a medialsector or central sector of SG in the C_1 and C_2 segment is devoid of ACP.7)Thegreat auricular nerve and the cutaneous cervical nerve were severed,the ACP activitywas absent in SG of the upper four cervical cords.The above results show that the primary sensory nerve of the peripheral nerveswhich supply the facial acupuncture points terminate in the SG of the subnucleuscaudalis of the trigeminal nerves and the dorsal horn and the primary sensory nervefibre of these nerves mostly terminate in C_1 and C_2 segment.

Objective To investigate the molecular basis for Jk(a b ) phenotype.Methods Routine serologic testing for phenotype.Genomic DNA covering 4～11 exons and partial introns of JK gene was amplified by ploymerase chain reaction.The PCR products were excised and purified from agarose gels with a kit,then fragments were directly sequenced.Results G mutated to A in the 3'acceptor splice site of intron 5;A to G at 78 site from the 3'end of intron 3;C to T at 84 site from the 5'end of intron 8; A to G at 588 site of exons ( exon 7); G to A at 838 site of exons (exon 9).The splice site mutation (G→A) of intron 5 may cause the skipping of exon 6.Conclusion G to A mutation in the 3'acceptor splice site of intron 5 maybe one of the molecular basis for Jk(a-b-) phenotype

Objective To identify the p phenotype. Method P blood group system was identified using p phenotype cells,anti PP 1 P k antiserum,and direct DNA sequencing.Result and Conclusion Proband was typed as p, with rare anti PP 1 P k in the serum,family study suggested that inheritance was autosomal recessive.

Objective To identify para-Bombay phenotype AB h m. Method ABO and H phenotype were typed. Absorption and elution were performed. Saliva was tested by inhibitory reaction. Direct sequencing was performed and family study was done. Results Proband was typed as rare para-Bombay phenotype AB h mand anti-H was detected in his serum. Family study suggested that the inheritance was autosomal recessive. Conclusion Rare AB h m phenotype was identified and anti-H has been detected in his serum.

0.05).Intracellular Ca2+ FI in rat ventricular myocytes induced by KCl was inhibited significantly in group B2 and B3 compared with that in group C(P