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Chronic Disease ; Consensus ; Heart Failure ; diagnosis ; therapy ; Humans ; Integrative Medicine

OBJECTIVE: To determine the cardiovascular actions of salusin-α in the caudal ventrolateral medulla (CVLM) in anesthetized rats. METHODS: Sixty-one anesthetic male SD rats were employed in the present study. The dose-dependant effects of salusin-α (0.04-4 pmol) on blood pressure and heart rate in the CVLM were determined by unilateral microinjection of salusin-α or artificial cerebrospinal fluid (aCSF) into the CVLM in 25 rats. In the other 36 rats, a CSF, kynurenic acid (Kyn), atropine, hexametho-nium (Hex) in the CVLM or a CSF/muscimol in the RVLM were given in advance to determine the mechanism of the cardiovascular actions of intra-CVLM salusin-α. RESULTS: Unilateral microinjection of salusin-a into the CVLM produced a dose-dependent hypotension and bradycardia. Prior administration of Kyn (1 nmol) or Hex (120 pmol) into the CVLM did not alter the hypotension and bradycardia induced by intra-CVLM salusin-α (P>0.05). But prior administration of atropine into the CVLM or pretreatment with muscimol within RVLM almost completely abolished the hypotension and bradycardia evoked by intra-CVLM salusin-α (P<0.05). CONCLUSION: Microinjection of salusin-α into the CVLM produces significantly hypotension and bradycardia, which probably originates from suppressing the activities of presympathetic neurons in the RVLM.

Objective To construct recombinant adenovirus carrying the gene of human somatostatin receptor subtype 2(SSTR2),to investigate its effect on growth of human pancreatic cancer xenografts in nude mice,and to further elucidate the underlying mechanisms.Methods The recombinant adenovirus coding for human SSTR2(Ad-SSTR2)and reporter gene LacZ(Ad-LacZ)were generated by site-specific recombination from Jun 1,2004 to Dec 10,2005.Human pancreatic cancer cell line was implanted subcutaneously into nude mice;normal saline(control group),Ad-LacZ(reporter gene control group)and Ad-SSTR2(experimental group)were injected into pancreatic cancer xenografts,respectively.The tumor volume and weight were measured.RTPCR and Western blot were used to determine the expression of SSTR2 after pancreatic cancer xenografts were infected with Ad-SSTR2.The expression level of ERK2 and ras proteins were assessed by Western blot.Results The virus titer of Ad-SSTR2 and Ad-LacZ was 6.0?1012pfu/L and 6.5?1012pfu/L,respectively.SSTR2 mRNA and protein were detected after Ad-SSTR2 infected pancreatic cancer xenografts.Growth of pancreatic cancer was significantly inhibited in the experimental group as compared with the control group.The growth inhibitory rate was 48.2%(P

Objective To investigate the clinical symptoms and imaging features of Budd-Chiari syndrome with hepatic vein obstruction (HVBCS) and the reasons of mistreatment. Methods Thirteen patients with HVBCS were misdiagnosed and mistreated as inferior vena cava (IVC) obstruction,including 8 patients treated with stent implantation in IVC once and 5 patients with balloon dilatation. After analysis of the clinical symptoms,signs and imaging features; hepatic vein obstruction was further confirmed by digital subtraction angiography (DSA)in all patients. Results All patients had variable degrees of portal hypertension and no apparent symptoms of IVC obstruction. CT or / and MRI showed obvious caudate lobe enlargement and DSA showed IVC narrowing with external compression. All patients were undertaken hepatic vein angiography including 4 with PTV and 9 with hepatic vein stent implantation. All patients' clinical symptoms and signs completely disappeared or markedly improved after the procedure. Conclusion The stenosis of IVC in HVBCS,caused by compression compensatory hypertrophy of hepatic caudate lobe can be cured by hepatic vein angioplasty which is the most correct and effective method.

Objective: To study the correlation of blood stasis syndrome or its accompanied syndromes with Gensini score in patients with coronary heart disease (CHD) in stable condition. Methods: The syndrome types of traditional Chinese medicine (TCM) and blood stasis score in 131 CHD patients confirmed by coronary angiography were recorded. Gensini score was calculated according to the coronary pathological characteristics showed by angiography. The correlations of blood stasis syndrome and its accompanied syndromes with coronary lesion and Gensini score were analyzed. Results: Among the TCM syndrome types, blood stasis, turbid phlegm and qi deficiency were the most common syndromes, revealed in 85 patients (64.9%), 83 patients (63.4%) and 85 patients (64.9%), respectively. The coronary lesion length and Gensini score in the patients with blood stasis syndrome were much higher than those in the patients with non-blood stasis syndrome (P<0.05 or P<0.01). In the subtypes of blood stasis, the coronary lesion length and Gensini score in the patients with blood stasis accompanied by turbid phlegm syndrome were higher than those in the patients with non-blood stasis syndrome (P<0.05). And in the patients whose blood stasis syndrome score was more than 9 points, the coronary lesion length was higher than that in the patients whose blood stasis syndrome score was less than 9 points (P<0.05). Besides, with bivariate analysis, the blood stasis syndrome score showed no correlation with Gensini score (Pearson correlation coefficient was 0.104, P=0.241). Conclusion: Blood stasis syndrome is the most common TCM syndrome in CHD patients in stable condition. The blood stasis syndrome score is proportional to coronary lesion length, and reflects the severity of coronary lesion.

0.05).One case developed hemorrhage of venous plexus in the posterior pedicle screw fixation group.And complications occurred in 6 cases(4 of superior nerve injury,1 of intra-incision hematoma and 1 of esophageal injury).There were significant differences between 2 groups(P

The effect of 5.8 ////mol L-1 propafeone on 0 phase depolarization of action potential were studed in meonatal and adult canine Purkinje fibres. Results showed: 1. in both groups, propafenone caused a reduction if APA and a decrease of Vmax; and the neonatal fibres were less sensitive to propafenone; 2. the inhibition of Vmax by propafenone showed frequency-dependent manner in both groups; and with theincrease of frequencies, the extent to which Vmax was inhibited became similiar in both groups. This sudy suggests that neonates were less sensitive to propafenone, but at higher frequencies neonates showed similiar responses as adults.

Objective To isolate and identificate a specific new gene highly related to human bladder cancer and to investigate the carcinogenesis mechanism and genetic susceptibility. Methods Normal bladder epithelia and cancer tissue collected from 8 cases of primary bladder transitional cell carcinoma were studied using the high performance?sensitive technique of mRNA differential display.The differential fragments were found by comparing with genetic expression. 6 of them were reamplificated?cloned?sequenced,and performed the autoploidy index in the public database of genbank. Results (1)37 differential fragments have been isolated.36 of which are expressed or highly expressed in cancer tissues,but not so or lowly expressed in normal tissues;while it has been expressed in 1 normal tissue but not in the cancer tissue.(2)Results of indexing:1 is homologous to TBX3 (T box gene),autoploidy 99%.1 is homologous to cl.Ca4 gene (member 4 of Calcium activated chloride channel gene family), autoploidy 99%.1 is homologous to Cu,Zn SOD gene (Cu,Zn superoxide dismutase),99%.The remaining fragments are not homologous to unknown sequences in the public database of genbank,assigned as YHHQ1,XHL,LHX. Conclusions (1)It is verified at genetic level the relation between the conment's increasing of Cu,Zn SOD in bladder cancer derived tissue and the occurrence,advance of bladder cancer.(2)The relation between TBX3,cl.Ca4 gene and human carcinoma has been observed.(3)YHHQ1 may be the new candidate of anti oncogene related to bladder cancer.XHL and LHX may be the new candidates of oncogene related to bladder cancer.

Objective To study anti-radiation behavior of plasma sprayed boron carbide coatings. Methods The anti-radiation capacity of 16Mn steel which was coated with 0.1 mm plasma sprayed boron carbide were studied. The irradiation beams were 6,10,15 MY X-ray and 6,9,12,15 MeV electron emitted by accelerator, X-ray emitted by 60Co machine,fast neutron, and X-ray emitted by kilovoltage X-ray ma-chine. Results Anti-radiation capacity of plasma sprayed boron carbide coatings was not found for X-ray beams emitted by accelerator and 60Co machine. For electron beams,the anti-radiation capacity were found. The deeper of location, the stronger was anti-radiation. However, the anti-radiation capacity was not good. For fast neutron,the anti-radiation capacity was good. For X-ray emitted by kilovoltage X-ray machine,the anti-radiation was good,and only 0.1 nun plasma sprayed boron carbide had 15% attenuation. Conclusions The plasma sprayed boron carbide coatings have the anti-radiation capacity for X-ray emitted by kilovoltage X-ray machine in medical field.