In vitro cellular adherence of murine neutrophils (Neu), macrophages (Mo) and eosinophils (Eos) to infective larvae (L3) of periodic Brugia malayi was promoted by immune serum (AIMS) or fresh normal mice serun (fNMS). Mo and Neu were more potent than Eos . in killing the L3. Eos could adhere to the L3 in the presence of AIMS or fNMS but a weak cy-totoxic effect was observed only when AIMS was used. The activity in AIMS could be removed with Staphylococcus aureus containing Protein-A (SPA) or anti-mice IgG antiserum suggesting that IgG might be in the observed cytotoxicity. Immunofluorescent staining revealed IgG binding to the surface of L3 after incubation with AIMS. Heat treatment of AIMS at 56℃ 30 min resulted in a reduced cytotoxic effect suggesting the role of complement in the reaction. EDTA but not EGTA abolished this activity indicating the operation of complement by alternate pathway. Scanning electron microscopic (SEM) studies comfirmed that the celluar adherence resulted in gross surface damage to the infective larvae.

Objective To investigate the prevalence of dyslipidemia and overweight/obesity of residents from different economic communities.Method One thousand six hundred and twenty-three residents from three communities with different economic condition in Huadu District,Guangzhou from June to July 2012 participated in the survey by questionnaire and health examination.Results The total prevalence rate in overweight/obesity was 29.4%and that of dyslipidemia 45.4%. The difference of prevalence rate of dyslipidemia among the three economic communities was not statistically significant(P>0.05). But the prevalence of overweight/obesity was statistically significantly different among them(P<0.05). The prevalence rate of overweight/obesity in Huadong town with a relatively poorer economy was lower than that of Tianmei town with a relatively better economy(P<0.017). The prevalence rate of overweight/obesity of male among the three communities had no significant differences(P>0.05),whereas the differences in the females were statistically significant(P<0.05),and the prevalence rate of Huadong town was lower than that of Tianmei town&Xinhua town. The differences in the prevalence of overweight/obesity at different ages from all economic communities showed statistical significance(all P<0.05).The residents aged 45~65 accounted for a large proportion of overweight/obesity.Conclusions Residents in the area have a high prevalence of overweight/obesity and dyslipidemia. The prevalence rates of overweight/obesity from the villages with better economic conditions are higher than that from the villages with poorer economic conditions. Residents aged 45-65 from all the three communities have a relatively high occurrence of overweight/obesity and dyslipidemia.

OBJECTIVE：To analyse the distributing proportion of amoxicillin/clavulanate K(CA) in β-lactmas and the popularzing rate of CA in hospitals.The proportions of different drug dosage forms of CA were compared.METHODS：The sums of money of the antiinfectives,β-lactams(penicillins) and CA consumed in 13 cities of China in 1999 were analysed.RESULTS：The distributing proportion of CA in β-lactams(penicillins)accounted for 18.3％ ,the popularzing rate of CA in 13 cities was 74.4％ ,of which,oral dosage form amounted to 41.7％ and powdered form for injection 58.3％ .CONCLUSION：The results can provide reference for rational use of drugs in hospital.

Objective To analyze the dynamic changes of white blood cells in patients with cavitary pulmonary tuberculosis (CPTB) during chemotherapy procedure ,and explore immunological indexes which could evaluate the curative effect and prognosis . Methods 85 cases of patients with CPTB (CPTB group) and 100 healthy people conducted physical examination (control group) were enrolled in this study ,The immunological indexes of white blood cells were detected and compared between CPTB group and control group ,and dynamic changes of white blood cells were analyzed in patients with CPTB .Results Compared with the control group ,absolute lymphocyte count and percentage of lymphocyte were decreased in the CPTB group before chemotherapy ,while the total white blood cells count ,absolute neutrophil count and percentage of neutrophil ,absolute monocyte count and percentage of monocyte were increased in the CPTB group before chemotherapy ,there were statistically significant differences between the two groups(P<0 .05) .Except the absolute lymphocyte count ,other indexes were statistically increased or decreased during chemothera‐py procedure .Conclusion After chemotherapy ,the immunological reactions induced by lymphocyte are under inhibitory state .The dynamic changes of white blood cells could reflect the state of cellular immunity in patients with CPTB during chemotherapy ,which could be helpful for the evaluation of curative effect and prognosis .

[Objective]To explore the consumptive disease treating experience in Gushi Yijing, written by Gu Songyuan who lived in the Qing Dynasty. [Methods]By studying consumptive disease related content in the book, discussing and summing up the consumptive disease treating experience. [Results] On the basis of pointing out seven kinds of error method that doctor treating consumptive disease at that time, Gu Songyuan put forward the opinion that consumptive disease was usual y caused by the internal injury of Zang and Fu organs which resulted into Yin deficiency as the main pathogenesis. The article contained other details as fol ows: the basic symptoms of consumptive disease of the five internal organs, three main therapeutic methods on treatment of consumptive disease, the preference of nourishment combined drugs with food, habit of using some sweet and cold durgs which can clear heat, the use of flesh and blood of sentient beings and so on. So the key idea of this article focused on the method of nourishing Yin to enrich Jing and blood which could cure the consumptive disease effectively.[Conclusion]Gu Songyuan inherited predecessor ’s theory, analysed the etiology of consumptive disease of the five internal organs, pointed out Yin deficiency is the main pathogenesis, and then put forward the therapeutic method, which has some medical knowledge on modern clinic.

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Child ; Dermatomyositis ; diagnosis ; Female ; Humans ; Male ; Middle Aged ; Otolaryngology ; Young Adult

Objective:To explore family environment of children with lea rning disorder.Method:153 children with learning disorder and 153 control were tested with FES(family environment scale),SCL-90 and PCCS(parentin g locus of control scale).Results:The scores of cohesion,expressive ness,achievement orientation,and intellectual-culture orientation were lower tha n control(p

Objective To investigate the clinicopathologic features and differential diagnosis ofacroangiodermatitis (AM).Methods Clinical and pathological data on 12 patients with AM were retrospectively reviewed.Results Clinical manifestations of AM consisted of circumscribed brown to violaeeous macules,plaques,nodules and ulceration.Lesions were located in bilateral legs in 6 patients,and in unilateral legs in the other 6 patients.Histopathological examination revealed an increased number of lobular or clump-shaped capillaries and small veins whose lumens were round and regular,swelling of vascular endothelial cells,and different degrees of erythrocyte extravasation,hemosiderin deposition,dermal fibrosis and sparse infiltrates of inflammatory cells.The lesions were histologically located in the superficial dermis in 3 cases,in the upper and middle dermis in 8 cases,and in the entire dermis in 1 case.Immunohistochemical studies showed that vascular endothelial cells stained positive for CD31 and CD34,while perivascular cells stained negative for CD34.Conclusions AM has specific clinical and pathological manifestations,and pathological examination is essential for the diagnosis of AM.

Objective To investigate the effects of dexamethasone on the growth of cervical cancer induced by HeLa cells transplanted into BALB/c mice. Methods HeLa cells were inoculated subcutaneously into BALB/c mice. Dexamethasone (15 mg?kg-1?d-1) was injected intraperitoneally from the 6th or the 16th day after tumor inoculation for 10 days continuously. Diameter of tumors was measured every two or three days. Apoptotic bodies were observed by transmission electron microscopy and counted by TUNEL. Results Dexamethasone repressed the growth of cervical cancer and induced tumor cell apoptosis, but the tumor volumes were of statistical difference between the two groups of different injection start time (P

Objective To investigate the effects of over physiological dose of dexamethasone on cervical carcinoma cell xenografted tumors in nude mouse.Methods HeLa cells were inoculated subcutaneously in BALB/c female mice aged 5-6 weeks.Over physiological dose of dexamethasone(62.5 mg?kg-1?d-1) was injected into the peritoneum from the 16th day after inoculation for 10 d.Diameters of tumor were measured every two or three days.The volumes and the inhibition rates of tumor were calculated.Apoptotic bodies were observed by transmission electron microscopy and counted by TUNEL.VEGF and TNF-? were detected by immunohistochemistry.Results In vivo,over physiological dose of dexamethasone can repress the growth of tumor and induce cell apoptosis.Compared with the control group,the tumor sizes were significantly smaller(P0.05).Conclusion Over physiological dose of dexamethasone can repress cervical tumor growth in vivo.This action may be related to apoptosis and VEGF,but have no relationship with TNF-?.