Objective To explore the impact of placenta polypeptide injection on fracture healing time and the incidence of infec-tion in patients with limb fractures .Methods 138 cases with limb fractures admitted to our hospital from February 2010 to Febru-ary 2012 were randomly divided into two groups ,and each group had 69 patients .The control group was given conventional treat-ment while the observation group was given placenta polypeptide injection on the basis of the conventional treatment .The bone healing time and infection rates were observed .Results The limbs fracture healing time of observation group was significantly shorter than that of control group (P<0 .05) .The infection rate of observation group (1 .45% ) was significantly lower than that of control group (10 .14% ,P<0 .05) .Conclusion The placenta polypeptide injection for treatment of limb fractures can effectively decrease fracture healing time ,and significantly reduce the rate of infection .

Child ; Hematopoietic Stem Cell Transplantation ; Humans ; Leukemia ; surgery

Objective To study the inhibitive effect of interleukin-4(IL-4) and (or) IL-10 on cell apoptosis of renal ischemic-reperfusion injury(IRI) in rats and its mechanism.Methods Thirty female Sprague-Dawley rats were divided into five groups(n=6): sham-operated group,operation+9 g/L NaCl control (NaCl group),operation+IL-4(IL-4 group),operation+IL-10(IL-10 group),operation+ IL-4+IL-10 (IL-4+IL-10 group). The animal model of IRI was set up by clamping bilateral renal pedicles for 45 min.When the clamp was loosed, relevant agents was injected in every group. Apoptosis of renal epithelial cells (TUNEL method) was obserued.Results IL-4 could not reduce level of apoptosis of renal epithelial cells.To some extent,IL-10 could reduce level of renal epithelial cells. The combination of IL-4 and IL-10 was more effective in inhibiting apoptosis of renal epithelial cells.Conclusion The combination of IL-4 and IL-10 is effective in inhibiting apoptosis of renal epithelial cells, indicating that it has a relationship in preventing renal histological and functional damage.

To observe the effects of sophoricoside and genistein on the biological characteristics of osteoblasts.

Objective To observe critical patients with thrombocytopenia,and exam their coagulation function,so as to diagnose disseminated intravascular coagulation(DIC)in the early stage.Methods Totally 56 critical patients complicated with thrombocytopenia were enrolled in the investigation;complete set of DIC tests were performed.A total of 26 cases were diagnosed as DIC according to the criteria from the International Society of Thrombosis Haemostasis(ISTH).The sensitivity,specificity,accuracy,likelihood ratio,predictive value of prothrombin time(PT),activated partial thromboplastin time(APTT),thrombin time(TT),fibrinogen(Fg),antithrombin Ⅲ and bleeding signs(including petechia and ecchymosia)were calculated by applying fourfold table.Results The specificity of PT prolongation and Fg reduction was 87.0%;the sensitivity of ATⅢ decrease,and each of PT prolongation,Fg reduction,and bleeding(including petechia and ecchymosis)were as high as 96.0%.Conclusion Multi-markers based on thrombocytopenia are likely to improve sensitivity and specificity of DIC diagnosis.

Adult ; Corticotropin-Releasing Hormone ; blood ; urine ; Diving ; physiology ; Helium ; Humans ; Male ; Oxygen ; beta-Endorphin ; blood ; urine

Adult ; Haploidy ; Hematopoietic Stem Cell Transplantation ; Hemorrhage ; Humans ; Lung Diseases ; Male ; Pulmonary Alveoli

[Objective]To study the mechanism of posterior longitudinal ligament in cervical spine through measuring the collagen and Proteoglycan and Calcium changes.[Method]Fifteen specimens of cervical longitudinal ligament from cervical spondylotic myelopathy(CSM)and ten control specimens from corpses without cervical spondylosis were obtained.The content of the collagen was measured by Weossner method.Collagen type Ⅰ and Ⅱ were measured by Enzyme-Linked Immunosorbentassay(ELISA)method.Phloroglucinol spectrophotometer to determine the change of amount of Proteoglycan.The Calcium by Methyl-Thymes-Blue(MTB)Colorimetric Method.The specimens were treated hy Hematoxylin and Eosin(HE)stain method and by Masson stain method,the pathological changes of two groups were observed through microscopy.[Result]In CSM,as compared to the control groups,there showed a decrease in the contents of the total Collagen,Collagen type Ⅰ and Proteoglycan,and increase in the content of collagen type Ⅱ.The rate of type Ⅰ/Ⅱ in CSM was lower than that in control groups.An increase in the content of the Calcium.All of which have statistic significance(P

The effects of cyanide poisoning on the contractibility of the cardiac muscle were observed after the dogs were infused intravenously with a lethal dose of NaCN (2.5 mg/kg) within 2 minutes through a cannulated femoral vein. Heart rate (HR) , left ventricular pressure (LVP) , the largest changing rate of LVP (LVP?dp/dtmax), cardiac muscle contractibility (CMC) , the largest changing rate of CMC (CMC?dc/dtmax) , and ECG changes were recorded. It was found that there was an initial excitatory response of cardiac functions in the first minute of NaCN infusion, and then a drastic cardiac failure occurred, which resulted in the rapid death of the dogs within 10 minutes after NaCN administration. The changes of myocardial contractibility were slower than those of .other cardiac parameters, which implies that cyanide can exert an excitation on the myocardium initially. Our results indicate that the effects of cyanide on myocardial contractibility may be one of the important factors to induce serious arrhythmia,which in turn results fatal cardiac failure.

Objective To discuss the effects of BuShenHuoXue on ovarian granulose cell apoptosis of DOS rats induced by GTW. Methods Sixty 12 -week -old female SD rats were randomly divided into 6 groups:control, model GTW,low dose of BuShenHuoXue Formula, middle dose of BuShenHuoXue Formula, high dose of BuShenHuoXue Formula, and LiuWeiDiHuang soup group. The ovary index, pathomorphological configuration,the level of E_2 and FSH in blood serum, and the expression of granular cell apoptosis protein Bcl - 2, Bax and Caspase - 3 was obserred after giving rats the different drugs separately. Results The expression of Bcl - 2, Caspase - 3 : as compared with control,the model, BuShenHuoXue low dose had a significant difference ( P