1.Retinal toxicity of repeated intravitreal injection with bevacizumab in diabetic rats
Chinese Journal of Ocular Fundus Diseases 2011;27(3):240-244
Objective To observe the retinal toxicity of repeated intravitreal injection with bevacizumab(Avastin)in diabetic rats.Methods Forty male Sprague Dawley(SD)rats were randomly divided into normal group(Group A,10 rats)and diabetes mellitus group(30 rats).The rats in diabetes mellitus group were induced with streptozotocin injection for diabetic retinopathy model.And then randomly divided into diabetic retinopathy(DR)group(Group B,10 rats),the rats were not intervened;the left eyes of the other 20 rats were intravitreal injected with bevaeizumab 3 μ1(25 mg/m1)for 3 times as experimental group(Group C);the right eyes of the 20 rats were not intervened as experimental control group(Group D),20 days after last intravitreal injection,retinal function was measured by Flicker Electroretinogram (F-ERG);retinal vascular pattern was determined by fluorescence microscopy of ethidium bromide(EB)stained retinal flat mounts;retinal morphological changes were determined by light microscope on hematoxylin-eosin (HE) stained sections;Thy-1 and VEGF expression was measured by immunohistochemistry staining.Results F-ERG showed that-the differences of a-and b-waves-the b-wave amplitude and the Ops-wave amplitude in the implicit time between group A,B,C and D were significant (F=33.165,36.162,19.955,23.243;P=0.000);the differences of a-wave amplitude between group A,B,C and D was not significant(F=0.097,P=0v961).Retinal blood vessel pattern was normalin Group A;retinal vascular vessels were tortuous and irregularly expanded in Group B:retinal vascular vessels of Group C were regular and thinner than Group A;microaneurysm were showed in Group D.Light microscope displayed that the layers of the rat retina of Group A were regular,the retinal architectures of Group B were irregular,the retinal layers were regular in Group C,the retinal layers were irregular in Group D.Immunohistochemistry staining discovered that Thy-1 and VEGF were mainly expressed in ganglion cell layer(GCL).Conclusion Repeated intravitreal injection of bevacizumab iS toxic tO retina of diabetes mellitus rats.
2.Research on Leweiyin Intervening SP and VIP of Rats with Functional Dyspepsia
Journal of Zhejiang Chinese Medical University 2006;0(02):-
[Objective]To disclose the mechanism of Leweiyin treating functional dyspepsia(FD) through experimental research. [Methods]Wistar rats are randomly divided into 7 groups: normal control, natural recovery, Madinlin, Shenling Baizhu pill, low dosage of Leweiyin, mediate dosage of Leweiyin, high dosage of Leweiyin, 6 in each group. Respectively administer to them with distilled water, Madinlin, Shenling Baizhu pill liquid, low, mediate and high dosage of Leweiyin at 2ml/100g, once every day, successively administer for 14 days one day after self model-making. Observe the gastric mucosa,measure gastro-intestinal hormones SP and VIP. [Results] Leweiyin can effectively increase SP and decrease VIP in experimental rats gastric antrum. [Conclusion] Leweiyin can increase gastro-intestinal tract excitement, regulate the contractility of gastro-intestinal smooth muscle, which may be the main mechanism for Leweiyin to treat FD.
4.Research about shorten preoperative fasting time for patients with digital cerebral angiography
Xumei YAN ; Xiaoqin XU ; Maohua CHEN
Chinese Journal of Practical Nursing 2010;26(5):22-23
Objective To know the feasibiliy and the effects of shorten preoperative fasting time for patients with digital cerebral angiography. Methods Divided 110 patients with digital cerebral angiogra-phy into the observation group and the control group according to their sequence enter the hospital. Routine fasting method was used in the control group, shorten fasting time was used in the observation group, com-pared the incidence rate of fasting-related complications between the two groups. Results The incidence rote of belly discomfort, thirstily, strvation and hypoglyeemic during the digital cerebral angiogrsphy in the con-trol group was significant higher than those of in the observation group, while there were no significant difference about the incidence rate of postoperative complication between the two groups. Conclusions For patients with digital cerebral angiography can shorten the preoperative fasting time properly, which can avoid certain complieaitons.
5.Changes of serum cortisol concentration in type 2 diabetic patients
Yan CHEN ; Zuohua XU ; Wen JIANG
Acta Universitatis Medicinalis Anhui 2001;(2):129-130
Objective To assay serum cortisol concentration in type 2 diabetic patients and evaluate the relationship between serum cortisol concentration and insulin resistance. Methods Serum cortisol concentrations were measured at 8 am, 4 pm and during oral glucose tolerance test(OGTT) in 60 newly-onset type 2 diabetic patients and 40 healthy subjects using RIA. Results ① Sernm cortisol concentrations were elevated in obese type 2 diabetic patients at 8 am and 4 pm as compared with non-obese patients and normal controls. ② Changes of serum cortisol concentration during OGTT were different between obese type 2 diabetic and non-obese patients: serum cortisol concentrations were decreased after glucose administration in non-obese patients but not in obese patients. ③ In type 2 diabetic patients, serum cortisol concentrations were negatively correlated with insulin sensitivity index (r=-0.34, P<0.01). Conclusion There is some degree of abnormal in cortisol secretion in obese type 2 diabetic patients.
7.Key issues on the clinical trial data management.
Ping-yan CHEN ; Yu XU ; Nan DAI
Acta Pharmaceutica Sinica 2015;50(11):1485-1487
This paper is prepared to discuss the common issues in data management, such as building and training of data management team, standard operation procedure, document management, execution and communication, strategies to correct and prevent mistakes, and measures to improve the quality and efficiency of clinical trials and data management.
Clinical Trials as Topic
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Data Collection
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standards
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Information Storage and Retrieval
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standards
8.Emergency repair of the facial soft tissue injuries with plastic surgery
Chuangang XU ; Yan CHEN ; Jing ZHAO
Chinese Journal of Medical Aesthetics and Cosmetology 2015;21(5):277-280
Objective To investigate the effect of cosmetic surgery on the facial soft tissue injuries.Methods The clinical data of 52 patients with facial soft tissue injuries from June 2013 to June 2014 were analyzed retrospectively.And plastic surgical techniques were applied to the early treatment of facial soft tissue injuries in patients,according to the size of defect and the degree of deformity of the patients,different plastic surgery treatment was chosen,such as skin flap or skin graft to repair wound surfaces.In this process,one must follow sterile noninvasive principle strictly with emphasis on the technique of plastic surgery such as entire debridement,wound healing application of skin flap and so on.Results Total 49 cases of facial soft tissue injuries were healed by first intention without significant complications,while 4 cases of them had mild scars.Absorbable suture exposition appeared in 3 patients after primary healing.Those sutures were romoved after disinfected and no sequelaes were happened.All patients were recovered with good appearances and functions to the most exent.Conclusions The treatment of facial soft tissue injuries,by applying plastic and aesthetic surgery,could reduce scar and postoperative deformity,and secondary corrective procedures can be avoided in most patients.
9.The research progress of traditional Chinese medicine inducing bone mesenchymal stem cells
Dongwei ZHANG ; Yan CHEN ; Xu LI
International Journal of Traditional Chinese Medicine 2015;(2):184-186
Through referring to relevant works and the latest literature, the application and research progress of the traditional Chinese medicine inducing mesenchymal stem cells were summarized. The researching results focusing on induction and proliferation of mesenchymal stem cell by salvia, notoginseng, tortoise plastron, pilose antler, ginseng, astragalus were introduced.
10.Therapeutic effect of desloratadine citrate disodium tablets combined with narrow band ultraviolet B in the treatment of pityriasis rosea
Rui YAN ; Aiguo XU ; Chen HUANG
Chinese Journal of Primary Medicine and Pharmacy 2015;22(2):224-225
Objective To observe the clinical efficacy of desloratadine citrate disodium tablets combined with narrow band ultraviolet B in the treatment of pityriasis rosea.Methods According to the digital table,88 pityriasis rosea patients were randomly divided into the two groups.45 cases of the study group were treated by desloratadine citrate disodium tablets combined with narrow ultraviolet B,43 cases of the control group were treated by desloratadine citrate disodium tablets alone.The both two groups applied Binghuangfule cream.The therapeutic effect and safety of the two groups were compared.Results The cure rate in study group was 71.11%,which of the control group was 51.16%,the difference was not statistically significant (x2 =3.69,P > 0.05).The effective rate of the treatment group was 91.11%,which of the control group was 69.77%,the difference between the two groups was statistically significant (x2 =6.43,P < 0.05).Conclusion The result reveals that desloratadine citrate disodium tablets combined with narrow band ultraviolet B is more effective and worthy to be used in clinical practice.