Protein fingerprinting technology(PFT) is a novel technology for laboratory diagnosis developed in recent five years.It has advantages of simple operation,testing quickly,high sensitivity and specificity.It is a revolutional progress for laboratory diagnosis.The application of PFT in medical field is mainly for the detection of diseases.The sensitivity and specificity in cancer detection are about 80%.Immunomic mass spectrometry(IMS) is a novel technology using the combined group antibodies for capture multi biomarkers and applying mass spectrometry to precisely analyze the modification or isoforms of the biomarker in single platform,whereas traditional assay could not be able to identify the variation of biomarkers.PFT and IMS have significantly influenced in cancer early detection,especially to evaluate cancers which did not express traditional tumor markers like AFP,CEA,etc.PFT and IMS have characteristics of early detection in the molecular and gene level.PFT and IMS are diagnostic technologies with bright future and potential applications.

Objective To investigate the effect of cisplatin on proliferation and invasion of colorectal cancer cell line SW480. Methods SW480 cells were used as the research object, and untreated SW480 cells were used as the control group. Different concentrations of cisplatin was given at different times to intervene. The MTT, Transwell chamber and phos-phate determination methods were used to detect proliferation, invasion and Na+-K+-ATPase activity expression level in SW480 cells. Results The physiological concentration of cisplatin (70 μmol/L) inhibited the proliferation of SW480 cells at 48 h. There was no significant difference in the inhibition rate at 48 h compared with 72 h and 96 h. Treatment with 70 μmol/L cisplatin for 48 h reduced the number of cells through Matrigel membrane matrix. The Na+-K+-ATPase activity was significantly increased in 35, 70 and 140 μmol/L cells after treatment with 17.5, 35, 70 and 140 μmol/L of cisplatin in SW480 cells for 48 h, and Na+-K+-ATPase reached the highest level at 70 μmol/L of cisplatin. Conclusion The decreased activity of Na+-K+-ATPase may lead to the attenuation in proliferation and invasion of colorectal cancer cells, which may be associated with cisplatin resistance in colorectal cancer cell SW480.

The clinical supervision of counseling and psychotherapy have a closely connection with clinical employment.Leading the way in clinical supervision,many clinical supervision models has been extrapolated by theories of various psychotherapy,relating to psychoanalysis theory,person-centered approach theory,cognitive and behavior psychotherapy theory,system theory,structuralism theory,and so on.Recently,especial,different clinical supervision models have to expand beyond psychotherapy,such as developmental model of supervision and society role model of supervision.The study has summarized clinical supervision models of the field,in order to offer literature index and concept frame.

Objective To study isolation and identification and culture of rat type A spermatogonial cells in vitro.Methods Percoll discontinue density gradient centrifugation combined with different speeds of different cells adhering to dish was used to purify the type A spermatogonial cells.The c-kit and TERT special antibodies were used to identify the type A spermatogonial cells.The purified cells were cultured in vitro. Results 0.614?10~6 cells per testis finally were obtained and the percentage of viable cells was 92.1% by trypan blue dye exclusion test.The percentage of type A spermatogonial cells expressing c-kit and TERT were 91.7?1.2% and 90.8?1.0% respectively.Type A spermatogonial cells could proliferate and self-renew in the DMEM containing 10% NBS.Conclusion Percoll discontinue density gradient centrifugation combined with different speeds of different cells adhering to dish is an efficiency method for isolation of rat type A spermatogonial cells.The purified cells are type A spermatogonial cells by identification of the immunohistochemistry of c-kit and TERT antibodies.Type A spermatogonial cells can proliferate and self-renew in vitro.

Objective To analyze the roentgenographic findings of the osteopetrosis. Methods Nine patients with clinically suspected osteopetrosis underwent the X-ray examinations of the chest,skull,pelvis,vertebral column,femur,tibiofibula,bones of upper limb and bilateral handbone. The roentgenographs of all patients were analyzed together with review of the literature. Results The basic image feature of osteopetrosis is bone density increased notably,cortible bone thickening,cavum ossis narrowing or occluded and mark X-ray signs include sandwich change in back bone,concentric rings change in pelvis,bone within a bone change,bright-dark alternation transverse striation in metaphysis of tubiform bone,baseball club appearance due to modelling deformity of long bones and periosteal new bone formation. Conclusion Osteopetrosis is a rare inherited disorder. Its mark roentgonographic findings are important evidence for performing the definite diagnosis and differential diagnosis.

Objective To investigate the value of multi-slice spiral CT(MSCT)in diagnosis of the causes of intestinal obstruction.Methods 49 cases of intestinal obstruction confirmed by surgery or clinical diagnosis underwent abdominal MSCT plain and enhanced scanning,three-dimensional post-processing reconstruction was done in 5 cases.CT features were analysed blindly by two senior radiologists,including the thickness,density and degree of enhancement of intestinal walls at the "transition zone" area of intestine and surrounding mesenteric structure.The causes of intestinal obstruction were analysed in combination with clinical history of the patients.Results The causes of intestinal obstruction were suggested by MSCT in 46 cases(94%),including intestinal adhesion in 13 cases,tumor in 11,hernia in 4,ischemic bowel disease in 4,gallstone in 2,gastrolith in 2,Bezoar in 4.paralytic ileus in 3 and appendiceal abscess in 3.Three cases were misdiagnosed.Conclusion MSCT is of significant clinical value in diagnosis of intestinal obstruction,which should be used as the first means in clinic practice.

Objective To explore the clinical characteristic of lab results and prognosis of severe intestinal virus infection dis -ease and to provide the objective basis for early intervention .Methods All children who were admitted in pediatric intensive care unit ( PICU) by laboratory diagnosis as cases of severe intestinal virus infection disease in Hunan Children 's Hospital from January 6 to No-vember 25, 2010 were selected as cases of this study .Their medical records were collected .The data was analyzed by χ2 test, Fisher's exact test , and binary logistic regression .Results A percentage ( 33.1%) of patients had abnormal white blood cell ( WBC ) counts, and the difference rates of younger group (under 1 year old) and upper 5 years old group were higher than that in 1~5 years old group with statistically significance (χ2 =12.13, P <0.01).The difference rates of aspartate aminotransferase (AST) and cardiac troponin I(cTnT) were 23.8%and 31.5%, respectively.A percentage (52.9%) of patients had reduced CD4 lymphocytes.Abnor-mal WBC, C reactive protein (CRP), and prognosis have no significant difference ( P >0.05).There were statistically significant differences in procalcitonin (PCT), AST, cTnT anomalies and prognosis ( P <0.01 or P <0.05).Conclusions Abnormal PCT, AST and cTnT are associated with the prognosis of children with severe intestinal virus infection , while WBC and CRP are not .

Objective To investigate the clinical value of combining early urine paraquat early clearance rate (UPCR) with severity index of paraquat poisoning (SIPP) in predicting the prognosis for paraquat poisoning patients.Methods In this retrospective research,a total of 425 cases diagnosed with acute paraquat poisoning from March 2014 to March 2016 in Emergency Intensive Care Unit,First Affiliated Hospital of China Medical University were enrolled.The general data of patients,the results of rapid qualitative test of paraquat in blood and urine,the concentration of paraquat in blood / urine,the poisoning time,the concentration of blood lactic acid and the APACHE Ⅱ score were collected.The early UPCR and SIPP were measured at different time intervals,and the ratio of 6-SIPP and 12 h-UPCR were calculated.These patients were divided into death groups and survival groups according to the 28-day mortality.The relationship between these factors and the mortality were analyzed.Results Of all the 425 patients,268 cases (63.1％) died,157 cases (36.9％) survived;the blood concentration of paraquat,the lactate concentration,SIPP values and the APACHE Ⅱ scores were significant difference between the two groups (P ＜ 0.05).The mortality of 2-6 hour paraquat rapid qualitative test result positive patients was higher (96.4％) than that of the negative patients (3.6％) (P ＜ 0.05);the mortality of 12-24 hour paraquat rapid qualitative test result negative patients was lower (11.5％) than that of the positive patients (88.5％) (P＜0.05).The 2-6 hour SIPP value was 19.8 ±6.7 in death group,which was higher than that in survival group (4.9±3.1) (P＜0.05);the 2-6 hour UPCR value was (41.7±9.3) indeath group,which was lower than that in survival group (86.3 ± 15.8) (P ＜ 0.05).There was no significant difference in the 2-6 hour UPCR value and 12-24 hour UPCR value between two groups (P ＞0.05).The 6 h-SIPP/12 h-UPCR value was (41.94 ±5.9) in death group,which was higher than that in survival group (5.27 ± 3.6) (P ＜ 0.05).Conclusion The combined use of early UPCR and SIPP values is an effective indicator of the prognosis of patients with acute paraquat poisoning and is helpful for the early stratification.We should pay more attention to the patients whose rapid qualitative blood test is positive because of their high mortality risk;for the patients whose 12 h urinequalitative test was negative,the hemoperfusion therapy might be stopped because the toxin was completely excluded,and the medical resources can be saved reasonably.The UPCR might indicate the excretion of toxins,and SIPP might indicate the severity of poising.

Objective To prepare monoclonal antibody (mAb) against recombinant human cardiac troponin I(cTnI).Methods The full-length gene encoding human cardiac troponin I (cTnI) was synthesized chemically and inserted into expression plasmid pBV220 to construct recombinant plasmid p pBV220/cTnI. The recombinant plasmid was transformed into E.coli DH5? which then expressed cTnI. The immunological activity of the expressed cTnI was analyzed by Western blot. Recombinant human cTnI protein was used as antigen to immunize BALB/c mice. Monoclonal anti-bodies against cTnI were prepared by normal hybridoma technology. The relative affinity of mAbs was determined by ELISA. Specificity of mAbs was analyzed by Western blot.Results Human cTnI gene was synthesized and confirmed by DNA sequencing. Positive recombinant clones were identified by restriction enzyme digestion analysis and DNA sequencing. Western blot analysis showed that the cTnI protein could be recognized by an anti- cTnI antibody. Two hybridmas producing antibodies against cTnI were obtained. IgG isotypes of two mAbs were IgG2a and IgG2b. Western blot showed that the antibodies were specific for cTnI. Neutralisation test showed that these mAbs could be evidently neutralized by cTnI.Conclusion The recombinant expression plasmid of cTnI was constructed successfully and expressed in E.coli. The method of EL ISA established to test serum cTnI is to clinically useful. The cTnI mAb which using cTnI as antigen prepared in this paper can be used for cTnI immunoassay in vitro.