Protein fingerprinting technology(PFT) is a novel technology for laboratory diagnosis developed in recent five years.It has advantages of simple operation,testing quickly,high sensitivity and specificity.It is a revolutional progress for laboratory diagnosis.The application of PFT in medical field is mainly for the detection of diseases.The sensitivity and specificity in cancer detection are about 80%.Immunomic mass spectrometry(IMS) is a novel technology using the combined group antibodies for capture multi biomarkers and applying mass spectrometry to precisely analyze the modification or isoforms of the biomarker in single platform,whereas traditional assay could not be able to identify the variation of biomarkers.PFT and IMS have significantly influenced in cancer early detection,especially to evaluate cancers which did not express traditional tumor markers like AFP,CEA,etc.PFT and IMS have characteristics of early detection in the molecular and gene level.PFT and IMS are diagnostic technologies with bright future and potential applications.

Objective To investigate the effect of cisplatin on proliferation and invasion of colorectal cancer cell line SW480. Methods SW480 cells were used as the research object, and untreated SW480 cells were used as the control group. Different concentrations of cisplatin was given at different times to intervene. The MTT, Transwell chamber and phos-phate determination methods were used to detect proliferation, invasion and Na+-K+-ATPase activity expression level in SW480 cells. Results The physiological concentration of cisplatin (70 μmol/L) inhibited the proliferation of SW480 cells at 48 h. There was no significant difference in the inhibition rate at 48 h compared with 72 h and 96 h. Treatment with 70 μmol/L cisplatin for 48 h reduced the number of cells through Matrigel membrane matrix. The Na+-K+-ATPase activity was significantly increased in 35, 70 and 140 μmol/L cells after treatment with 17.5, 35, 70 and 140 μmol/L of cisplatin in SW480 cells for 48 h, and Na+-K+-ATPase reached the highest level at 70 μmol/L of cisplatin. Conclusion The decreased activity of Na+-K+-ATPase may lead to the attenuation in proliferation and invasion of colorectal cancer cells, which may be associated with cisplatin resistance in colorectal cancer cell SW480.

The clinical supervision of counseling and psychotherapy have a closely connection with clinical employment.Leading the way in clinical supervision,many clinical supervision models has been extrapolated by theories of various psychotherapy,relating to psychoanalysis theory,person-centered approach theory,cognitive and behavior psychotherapy theory,system theory,structuralism theory,and so on.Recently,especial,different clinical supervision models have to expand beyond psychotherapy,such as developmental model of supervision and society role model of supervision.The study has summarized clinical supervision models of the field,in order to offer literature index and concept frame.

Objective To investigate the dose response relationship for K562 cells' loss of proliferation ability induced by 60Co γ ray irradiation. K562 cell morphological change and Egr-1 expression level are also investigated. Methods 3H-TdR incoporation assay, Giemsa Staining, RT-PCR were used in this study.Results It was noted that the proliferation inhibition is positively dose-dependent. K562 cells showed extensive changes in both morphology and Giemsa staining characteristics after radiation exposure. The irradiated cells acquired characteristics which the high differentiated cells had only. In the early stage after exposure to 5 Gy 60Co γ ray irradiation. A transient transcriptional upregulation of Egr-1 gene which was related to apoptosis and differentiation was observed.The transcriptional level reached its top on time point of 0.5 ～1 hours after exposure. Eight hours after exposure, the transcriptional level was below normal.Conclusions The proliferation inhibition of K562 cell is positively dose dependent. Egr-1 gene can be rapidly activated after exposure to 5 Gy 60Coγ ray. After exposure to 60Co γ ray irradiation, K562 cells entered into a more mature stage on differentiation of morphological

OBJECTIVE:To analyze the characteristics and rules of pediatric adverse drug reaction (ADR) in our hospital, and provide reference for clinical safe drug use. METHODS:Retrospective study was adopted to statistically analyze the ADR re-ports in our hospital from Mar. 2012 to Jun. 2016. RESULTS:In the 176 ADR reports,102 were male(57.95%)and 74 were fe-male (42.05%);children younger than 6 years old had the highest incidence of ADR (81.26%);intravenous administration in-duced 153 cases(86.93%),the top one was antibiotics,which involving 20 antibiotics,totally 106 cases(60.23%);skin and its appendages damage were the main manifestations, totally 127 cases (50.60%). CONCLUSIONS:Pediatric ADR monitoring should be strengthened,dinical rational drug use should be promoted by cooperation in many ways to reduce and prevent the incid-erle of pediatric ADR.

[Objective]This paper will pitch-in TCM psychosomatic theory(from Huangdi Neijing),exploring the disorders of depression with the metabolic syndrome and pathogenesis of traditional Chinese medicine,so as to provide effective ideas for the treatment of depression with metabolic syndrome.[Methods]:Through expansion on traditional chinese medicine's basic understanding of depression with metabolic syndrome,utilizing basic TCM viscera-state theory, from the causes of this disease,key factors,pathogenesis transformation three aspects to discuss the pathogenesis of the disease. [Results]By discussing the heart,liver,spleen function of zang-fu organs and their mutual relations in the role and impact of the disease development process, this paper thinks of the nature of disease as asthenia in origin and asthenia in superficiality,which locates in spleen,has association with heart and liver, phlegm, qi and blood stasis is the disease of pathological product of pathological factors to promote the development of the disease.Kidney would be involved in it with long course of disease.The therapy could focus on tonifying spleen,nourishing heart and soothing liver,with methods of promoting the circulation of qi,removing blood stasis and reducing phlegm,meanwhile looking out to protect kidney.[Conclusion]Disorder of depression and pathogenesis of TCM of metabolic syndrome,to guide the clinical treatment of the disease and the theory of traditional Chinese medicine development has practical significance.

Objective To investigate the influence of laparoscopic cholecystectomy (LC)versus open cholecystectomy (OC)on postopera-tive systemic infection and immune response in patients with acute cholecystitis complicated by choleperitonitis.Methods A prospective randomized controlled trial was performed for 45 patients who had a definite diagnosis of acute calculous cholecystitis complicated by cho-leperitonitis in Shanghai Liqun Hospital from January 2014 to June 2016.According to surgical procedures,the patients were randomized in-to LC group (23 patients)and OC group (22 patients).The length of hospital stay,postoperative complications,and deaths were evaluated in both groups.Blood samples were collected from all patients before surgery and at 1,3,and 6 days after surgery to compare the changes in neutrophil count,serum levels of C -reactive protein (CRP)and interleukin -6 (IL -6),and erythrocyte sedimentation rate (ESR),as well as the incidence of endotoxemia.The t -test was used for comparison of continuous data between groups,and the chi -square test was used for comparison of categorical data between groups.Results The LC group had a significantly shorter length of hospital stay than the OC group (5.4 ±2.7 d vs 10.2 ±3.5 d,t = -5.46,P <0.001).One patient (4.3%)in the LC group and 6 (27.3%)in the OC group ex-perienced peritoneal abscess after surgery,and there was a significant difference in the incidence rate of complications between the two groups (χ2 =4.77,P =0.03).In all patients,the mortality rate was 17.8% (8 /45),with 1 (4.3%)in the LC group and 7 (31.8%)in the OC group,and there was a significant difference between the two groups (χ2 =5.16,P =0.02).Of all patients in the OC group,4 died of peritoneal abscess,1 died of pulmonary embolism,and 1 died of myocardial infarction;of all patients in the LC group,1 died of my-ocardial infarction.There were no significant differences in inflammatory markers before surgery between the two groups.At 1,3,and 6 days after surgery,the LC group had significantly lower neutrophil count,serum levels of CRP and IL -6,and ESR (except at 1 day after surger-y)than the OC group (all P <0.05).Furthermore,the OC group had a significantly higher concentration of endotoxin than the LC group (P <0.05),but the level of endotoxin returned to normal at 2 days after surgery in both groups.Conclusion Compared with OC,LC can reduce the probability of endotoxemia,help with the establishment of immunological defense,and reduce the risk of postoperative infection.

Camptothecin/*pharmacology ; Liver Neoplasms, Experimental/*immunology ; Neoplasm Transplantation ; Sarcoma, Experimental/*immunology ; Transplantation, Isogeneic

Objectives To provide outline for the Research & Development department of hospitals making research strategy by analyzing the quantity of publications and their cited status for the top 20 hospitals in SCI and MEDLINE, and the top 100 hospitals in domestic publications in 2008. Methods The number of publications and their cited times for the first 20 hospitals in SCI and MEDLINE, and the top 100hospitals in domestic publications in 2008 were analyzed with bibliometric analysis. Results Compared with 2007, the increased number and ratio of publications in SCI and MEDLINE for the top 20 hospitals were 1050, 51.19% and 658, 15.04%, respectively; for top 100 hospitals, the average decreased number of domestic publications was 2.08, and the number of cited publications was increased 202.52 and the increased ratio was 13.51%. Conclusions The SCI publications from top 20 hospitals increase sharply, the increase of the MEDLINE articles from the top 20 hospitals is slowly. The negative growth is showed in the number of domestic articles for the top 100 hospitals. In addition, the increase of cited articles is slowly and disproportion.

Objective The quantitation measurement of microalbuminuria was adopted to verify the feasibility of urinary microalbumin and ratio of mieroalbumin to creatinine (M/C) detection with dry hemieal method. Methods Totally 80 urine samples from the patients with clinically diagnosed dia-betes were tested by the immune quantitation method (immunoturhidimetric assay) and dry heroical method (HT2000 urine analyzer and test paper, Guilin Hnatong Company) simultaneously. Sarcosine oxidase method was applied to measuring creatinine level. Ten cases of outliers were removed. With the quantitation result of M/C as the reference standard, immune quatilyzation of the M/C as refer-ence, we compared and analyzed the results of the immune quantilization of urine microalbumin, those of the dry chemistry method and of the dry chemistry system detecting M/C. Results There was sig-nificant differences in test results of urinary microalbumin with the dry chemistry method and the im-mune quantitation method (P<0.01) The sensitivity, accuracy and Youden index of the microalbu-minuria testing had been observed to decrease in order in immune the dry chemistry method, the im-mune quantitation method and M/C quantitation detection method. Conclusion The sensitivity of semi-quantitation dry chemical method is satisfactory in detecting microalbumin, which may be used as a means of microalbumin screening. M/C detection with the dry chemical method isn't suitable for screening microalbumin in instant urine samples.