Objective To use SVV as a standard and observe the availability of IDVG in assess-ing volume status of perioperative esophageal cancer patients.Methods Thirty patients undergoing selective esophageal cancer surgery were enrolled.The values of SVV were obtained from Vigileo sys-tem after tracheal intubation.Baseline blood glucose was measured through radial artery,and then 5 g glucose (50% glucose solution 10 ml)was injected through right jugular vein in 30 s.Blood glucose levels in 1,3,5,60 mins after the injection were assassed via radial artery blood samples and IDVG3 (3 min post injection of glucose)was further calculated.The correlation between SVV and IDVG3 was studied using Pearson correlation analysis.Results There's no difference in blood glucose level CT0 and CT60 [(5.60±0.74)mmol/L vs (5.77 ± 0.76 )mmol/L].There was a negative correlation be-tween SVV and IDVG 3 mins after glucose injection (r =-0.79,P <0.001).Conclusion IDVG of 3 min post injection of glucose can predict fluid volume state in patients with esophageal cancer.

Objective:To study the effect of quercetin given during pregnancy and lactation period of obese rats on weight and expression of insulin-like growth factors-1 ( IGF-1 ) mRNA of the offspring . Methods:In the study , 8 healthy weaning female SD rats were randomly selected to feed the basal diet and as blank control group , and the others were fed with high-fat diet.When the average weight of the high-fat diet rats was 20% as many as the average weight of the control group , we believed the model succeeded .Then the female SD rats were mated and the pregnant rats were randomly divided into 5 groups , 8 rats in each .They were fed with high fat diet during pregnancy and lactation , and the obese pregnant rats were respectively irrigated with 0 mg/kg body weight ( high-fat control ) , 50 mg/kg body weight,100 mg/kg body weight , and 200 mg/kg body weight quercetin .The blank control group was fed with basic diet throughout the experimental period .The birth weight , the weights during the development of d7, d21, d56, and d98, and the expressions of IGF-1 mRNA in livers tissue of the F1 generation were measured.Results:The average birth weight of the F1 generation of high fat diet (HFD) group was sig-nificantly higher than that of the blank control group .Decreased offspring birth weight was observed with the intervention of quercetin , which effect remained to the delectation .The quercetin also reduced the ex-pression of IGF-1 mRNA in livers of the F1 generation , and this effect remained to the adulthood .The 200 mg/kg body weight quercetin was most significantly effective .Conclusion:The averaged birth weight of the F1 generation of obese pregnant rats was significantly increased , and quercetin could effectively in-hibit the expression of IGF-1 mRNA in livers of F1 generation to decrease cell proliferation and cell differentiation.

OBJECTIVE： To develop a method of thin－ layer chromatographic scanning to determine the content of puerarin in Jiangtang pill METHODS： Silica GF254 thin－ layer was used， and the mobile phase consisted of chloroform－ methanol－ water(7∶ 2 5∶ 0 25) with the detection wave－ length of 254nm and the control wave－ length of 370nm RESULTS： The linear range of puerarin was 0 4～ 2 0μ g The mean recovery was 99 40％ (n=5) with RSD of 1 37％  CONCLUSION： This method is accurate， reliable and can be used for the determination of puerarin in Jiangtang pill

Objective To investigate the clinical effect of superselective arterial infusion chemotherapy(SACT) and embolization in advanced cervical carcinoma patients before surgery. Methods 26 previously untreated patients with stage ⅡA-Ⅳ cervical carcinoma admitted from January 2004 to March 2008 were enrolled into the study, patients underwent SACT of the internal iliac artery(dose of the drug: CBP 80 rag, VCR 2 rag, MMC 16 mg) by Seldinger technique in the surveillance of DSA, the obvious branches of the tumor blood supply were embolized by gelfoam particles. Size of tumor before and after chemotherapy was measured by gynecological examination combined with B ultrasonic examination and CT, then calculated the percentage of tumor regression. Radical hysterectomy was administered one month after chemotherapy. Results 24 patients were evaluated as PR, the reaction rate of chemotherapy was 92.3 %. Conclusion SACT before surgery can effectively reduce tumor volume, decrease lymphatic metastasis and subclinical dissemination and enhance the opportunity of radical hysterectomy, and to improve the quality of life and OS of the advanced cervical carcinoma patients.

OBJECTIVE To study the influence factors acted on germicidal efficacy of a bed unit ozone sterilizer. METHODS All the experiments were carried out according to the Technical Standard for Disinfection. RESULTS After working for 20min,the concentration of ozone in disinfection packet attained to 765.21 mg/m~3,the killing rates of Staphylococcus aureus were 90.96%,95.46% or 99.35% under the relative humidity(RH) of 50%,70% or 90% at 20℃,the killing rates were 95.56%,68.36% or 66.42% at 20℃ and RH 70% in the presence of 0%,25% or 50% organic substances,the temperature and carriers had little influence on killing rate. CONCLUSIONS Considering the strong influences of relative humidity,and organic substance on ozone sterilizer,we should increase the concentration of ozone or improve the conditions of disinfection in actual use to assure the germicidal efficacy.

Objective To summerize clinical application of the trifoliated flap from the dorsal foot. Methods The flaps,which were made of the first dorsal metatarsal flap, medial foot flap and lateral foot flap,were designed on the basis of the anatomy of flap whose common pedicle was anterior tibial artery or dorsal foot artery and whose other branches was medial tarsal artery or medial anterior, melleolar artery and lateral tarsal artery respectively,the single flaps were harvested to repair multi-finger skin defect.The flaps from both feet were repaired digit degloving injuries. Results Thirty-one patients were treated by trifoliated flap from the dorsal foot.29 of 31 flaps survived completely.Two flaps in patient necrosised.One flap in two patients necrosised. Conclusion The trifoliated flap from the dorsal foot is a good procedure for coverage problems in multiple-finger avulsion and digit degloving in juries.

Objective:To probe the effect of prenatal ethanol exposure on the mitochondrial protein expression in fetal mouse cerebrum. Methods:Pregnant CD-1 mice were given 5.0 g/kg ethanol intragastrically from pregnant days (PD) 6-15. Fetal cerebral mitochondria were isolated on PD 18. The overall mitochondrial protein was applied to two-dimensional gel electrophoresis. Differentially expressed protein spots were cut off and identified by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Activities of respiratory chain complex Ⅳ(72.3%?4.6% of control)and ATP synthase, and ATP content in the fetal cerebral cells were measured. Results:Expressions of some constructional and functional proteins were altered by in utero ethanol exposure. Activities of respiratory chain complex Ⅳ and ATP synthase (80.3%?5.1% of control)were both decreased. ATP content (67.9%?3.9% of control) in the cerebral cells was reduced in the in utero ethanol exposure fetuses. Conclusion:In utero ethanol exposure affects the mitochondrial protein expression of fetal mouse cerebrum, even in the tissue without obvious morphological malformations, which may be a possible mechanism of behavioral defects found in children with prenatal ethanol exposure.

Objective Mobile operating compartment with overpressure protection can provide a safety environment for the patients and the surgical team by minimizing the risk of biochemical contamination through appropriate air filtration, steady overpressure foundation and air distribution scheme. Methods The air movement and the temperature distribution inside the mobile operating compartment with the overpressure protection performance were simulated by the technique of computational fluid dynamics(CFD), the air pattern and the temperature distribution were analyzed. Results The air velocity was under 0.5m/s in the most zone of person activity and the air velocity were distributed uniformly in the mobile operating compartment under the condition of the overpressure protection system. The obvious temperature gradient didn't exist in the vertical plane of the mobile operating compartment. The temperature distribution in the mobile operating compartment was quite uniform and the temperature was almost at 27℃ with the temperature difference of almost 2℃. Conclusion The human thermal comfort is good in the mobile operating compartment and the environment in the mobile operating compartment can satisfy requirements of the operation.

Objective To design and produce a kind of NBC filtration and ventilation unit with the function of filtering biological aerosols and chemical poisonous gas and so on in the air,providing new clean air for the medical shelter.Methods The filtration unit and ventilation unit were united in principle of manipulability and repairability by modularization method.Results Structural dimensions and vibrational noises of the unit were reduced,and manipulability and repairability were improved.Conclusion The unit can meet the demands of biological and chemical protection for the medical shelters,and the structural design of fast fitting and so on can meet the requirements of fast responses.

extensive injury group.The intelligence quotient level of left frontal lobe group was lower than that of the right;PIQ of right temporal lobe group was lower than that of the left,VIQ of right temporal lobe group was higher than that of the left(P