Objective To investigate the antimicrobial resistance of clinical isolated acinetobacter baumannii against the common antimicrobial agents in Huainan and to direct rational medication in clinical.Methods 160 acinetobacter baumannii strains were isolated from 3 hospitals in Huainan from Oct.2010 to Jun.2012.Bacteria identification and antimicrobial susceptibility testing were detected by VITEK32 of BioMérieux company.Cefoperazone sulbactam susceptibility was detected by Kirby-Bauer method.Results The drug resistant rate of acinetobacter baumannii against 10 antimicrobial agents was beyond 50.0％ and resistant rate of aztreonam was up to 90.1％.The drug resistant rate of only 4 antimicrobial agents was lower than 50.0％.In these 4 drugs,the lowest drug resistant rate was cefoperazone sulbactam (23.4％) and antimicrobial resistant rate of imipenem and meropenem was 26.0％ and 31.2％,respectively.Conclusion The antibiotic resistance and multiple resistance of acinetobacter baumannii in Huainan is serious.Cefoperazone sulbactam and imipenem are the first drugs for clinical therapy of acinetobacter baumannii infection.

OBJECTIVE: To establish a 29 Y-STR loci multiplex PCR system for investigating the genetic polymorphisms and to assess its application value in forensic science. METHODS: A multiplex PCR system was established using a five color fluorescence labeling 29 Y-STR loci (DYS456, DYS389 I , DYS437, DYS447, DYS389 11, DYS438, DYS522, DYS460, DYS458, DYS622, DYS390, DYS392, DYS448, DYS449, DYS391, Y-GA TA-H4, DYS388, DYS19, DYS385a/b, DYS527a/b, DYS393, DYS459a/b, DYS635, DYS439, DYS570 and DYS627) for multiple amplification and capillary electrophoresis. And its applicability was validated with genetic polymorphism data of 29 Y-STR of unrelated 2,000 male samples in Shandong Han population. RESULTS: A total of 1,981 different haplotypes of 2,000 individuals showed genotype diver- sity between 0.370 0 and 0.965 4. The system provided stable and accurate typing with high sensitivity of 0.05 ng. It satisfied the needs of variety of routine biological samples. CONCLUSION The 29 Y-STR loci multiplex PCR system could be applied for actual cases and establishment of Y-STR database. In addition, it has great significance in forensic science practices and related research.
Asian People/genetics* ; China ; Chromosomes, Human, Y ; DNA/isolation & purification* ; Ethnicity/genetics* ; Forensic Genetics/methods* ; Forensic Sciences ; Genetics, Population/methods* ; Haplotypes ; Humans ; Male ; Microsatellite Repeats ; Multiplex Polymerase Chain Reaction/methods* ; Polymorphism, Genetic ; Reproducibility of Results

The mechanism and interaction among Rb/p16, Rb/E2F1 and HDAC1 proteins in gallbladder carcinoma were investigated. By using the immunoprecipitation method, the interactions among Rb, p16, E2F1, HDAC1 proteins in gallbladder carcinoma cell line (Mz-ChA-1) were studied. It was found that there were Rb and E2F1 proteins in the precipitates with anti-HDAC1, and there were HDAC1 and E2F1 proteins in the precipitate with anti-Rb. It was concluded that there are specific interactions among Rb, HDAC1 and E2F1 proteins in gallbladder carcinoma, indicating the existence of the direct Rb/E2F1/HDAC1 signal transduction pathway. There is no direct relationship between p16 proteins with Rb, HDAC1, and E2F1 proteins.

? Choroidal neovascularization is the primary pathogenesis of neovascularage - related macular degeneration ( nAMD ) , and the role of vascular endothelial growth factor ( VEGF ) in neovascularization has been widely recognized. Currently, drugs target different targets of VEGF have been widely used in the treatment of nAMD. As a subtype of VEGF, placental growth factor ( PlGF) has synergistic effects with VEGF-A on promoting angiogenesis, stimulating the migration of endothelial cell proliferation and mediating immune inflammatory response. There is no expression of PlGF in mature blood vessels so PlGF hashigh specificity. ln this paper, the role of PlGF in the pathogenesis and treatment of nAMD is reviewed.

Objective To investigate the differences of the plasma homocysteine (Hcy) between the patients with acute cerebral infarction and the normal controls, and the relationship between the levels of plasma Hcy and folic acid, vitamin B_(12) and lipids, and the relationship between clinical symptoms in patients with acute cerebral infarction. Methods A total of 91 patients with first-ever acute cerebral infarction and 100 controls without cerebrovascular accident were included. Their ratio factors such as age, sex, hypertension and diabetes did not have significant differences. Enzymatic cycling was used to detect plasma Hcy levels. Chemiluminescence was use to detect folic acid and vitamin B_(12) levels. Biochemical analyzer was used to measure lipid levels. The patients with acute cerebral infarction were evaluated by the National Institutes of Health Stroke Scale (NIHSS). Results The plasma Hcy level was significant higher in the acute cerebral infarction group than that in the control group (21.22 ±7.29 μmol/L vs. 13.19 ± 2.13 μmol/L) (P ＜ 0.05); the plasma Hcy level in the acute cerebral infarction group was significantly negatively correlated with folic acid (r = - 0.307,P ＜0.05) and vitamin B_(12) (r = - 0.270, P ＜0.05). It was significantly positively correlated with low-density lipoprotein (r =0.282, P ＜0.05), and it was significantly negatively correlated with high-density lipoprotein (r = -0.219, P ＜0.05). The mean value of the plasma Hcy in acute cerebral infarction group increased with the increase of NIHSS scores. Conclusions Hyperhomocysteinemia is an independent risk factor for acute cerebral infarction. The levels of folic acid and vitamin B_(12) decreased with the increase of Hcy levels. The higher the Hcy levels,the more serious the clinical symptoms are. Hcy may increase the risk of ischemic cerebrovascular disease by influencing the lipid metabolism.

Postoperative peritoneal adhesion represents a major complication of surgery. Recently, the angiogenesis which cyclooxygenase-2 enzyme induced was found to play an important role in the adhesion synthesis. This review summarized the relationship between COX-2 induced angiogenesis and peritoneal ad- hesion.

The effects of electrical and chemical stimulation of the vertral medial area of nucleus facialis (vMNF) on the myoelectric activities of genioglossus were observed in 26 urethane-anaesthetized and vagotomized rabbits. The results are as follows: (1) Long train electrical stimulation at the vMNF inhibitited the myoelectric activities of genioglossus markedly. (2) Microinjection of glutamate into the vMNF caused inhibitory response of the myoelectric activities of genioglossus. (3) When single pulse electrical stimulation on vMNF, measurement latency of genioglossus myoelectric activities was (20.6±0.4)ms. These results suggested that the excitation of vMNF could decrease the myoelectric activities of genioglossus so that the resistance of upper airway might be enhanced.

Child ; Humans ; Inflammatory Bowel Diseases ; diagnosis

Objective To study the risk factors in elderly moderate-severe thermal burn patients with acute ischemic stroke (AIS).Methods Forty-two elderly moderate-severe thermal burn patients with AIS served as an observation group and 47 elderly moderate-severe thermal burn patients without AIS served as a control group in this study.Risk factors in thermal burn patients with AIS were analyzed by univariate and multivariate logistic regression analysis respectively.Results The number of neutrophils and platelets,hematocrit,serum levels of fibrinogen,thromboxane A2,creatinine,procalcitonin,C-reactive protein,TNF-α and interleukin-6 (IL-6) were significantly higher while the systolic blood pressure (SBP) on admission was significantly lower in observation group than in control group (P＜0.01).Logistic regression analysis showed that SBP on admission,serum levels of fibrinogen,thromboxane A2,TNF-α and IL-6 were the independent risk factors in elderly moderate-severe thermal burn patients with AIS (OR =1.106,95％CI:1.007-1.501,P=0.007;OR=3.316,95 ％ CI:1.251-8.792,P=0.016;OR=1.077,95％CI:1.003-1.156,P=0.041;OR=2.901,95％CI:1.109-2.799,P=0.013;OR=1.502,95％CI:1.581-2.031,P=0.021).Conclusion SBP on admission,serum levels of fibrinogen,thromboxane A2,TNF-α and IL-6 are the independent risk factors in elderly moderate-severe thermal burn patients with AIS.

Objective To retrospective analyze the treatment of hallux valgus with the procedure of osteotomy of the base of the first metatarsal, combining with osteotomy of the base of the second and/or third metatarsal(s), and to study the indications and effects of this procedure. Methods 35 cases 56 hallux valgus feet were treated by operative procedure from January 1994 to December 2003. 26 cases 43 feet underwent the operative procedure of osteotomy of the base of the first metatarsal, 9 cases 13 feet with painful callus under the second and/or third metatarsal(s) head underwent osteotomy of the base of the first, second and/or third metatarsal(s). The axial and lateral films of all feet with loading were taken before and after operation. The change of anatomic indexes and AOFAS of the patient postoperatively were recorded and analyzed. Results In the group with osteotomy of the base of the first metatarsal, AOFAS score was 47.6?5.8 preoperatively, and 84.3?5.7 postoperatively. In the group with osteotomy of the base of the first, second and/or third metatarsal(s), AOFAS score was 44.7?5.7 preoperatively, and 85.7?4.5 postoperatively. There were significant differences between the preoperative and postoperative rontgenographic index and AOFAS in each group. Conclusion The operative procedure is effective. The operative procedure of osteotomy of the base of the first metatarsal can get good result in moderate and severe hallux valgus patients. Osteotomy of the second and/or third metatarsal(s) were recommended in cases with painful callus under the second and/or third metatarsal(s). Normal forefoot appearance and function can be restored by the procedure to reestablish the transverse arch.