Objective To investigate the effect of amyloid on the expression of kinesin superfamily(KIFs) genes in cortex of Wistar rats. Methods The animal model of Alzheimer's disease was prepared by injection of amyloid into brain ventricle of rats.The learning impairments were verified by water maze test.The levels of mRNA for six kinesin superfamily genes,kinesin,KIFlb,KIFlc,KIF3a,KIF3c,and KIF4,in cortex of rats,were estimated by real-time RT-PCR. Results Two weeks after amyloid injection,the rats showed significant learning impairments.In cortex,the expression of KIF genes was increased after amyloid injection.Conclusion\ The increase of KIF gene may be an evidence for the excitotoxity of amyloid to neurons.

Objective To explore the expression of cell apoptosis regulatory elements in esophageal canceration course. Methods 60 specimens of esophageal carcinoma and corresponding adjacent no cancerous tissue were detected quantitatively by propidium iodide(PI) stain and indirect immunofluorescence of fluorescein isothiocyanate(FITC) using flow cytometry(FCM) equipment. Results Expression of bcl-2 and c-FLIP in esophageal carcinoma was significantly higher, and Fadd, Caspase-8 and Caspase-3 were significantly lower than that in adjacent tissues (P <0.01). There was positive correlation between Fadd and Caspase-8 gene (P <0.01 or P <0.05). The expression of Fadd was positively correlated to Caspase-8. Comparing with normal epithelium and dysplasia tissue, DNA content and heteroploid cells were increased significantly in esophageal carcinoma. Conclusion During the esophageal canceration course, expressions of relevant cell apoptosis regulatory elements were abnormal. DNA content and heteroploid cells were increased significantly in esophageal carcinoma.

Objective To investigate the moderator of children' s perceptions quality of parental involvement on parenting style and children' s sport expectancy-value beliefs.Methods 595 students of grade 2 to 6 from five elementary schools were chosen and analyzed.Data were analyzed by SPSS16.0 software packet using independent sample t test,One-Way ANOVA,correlation analysis and hierarchical regression statistical analysis of regulation.Results Children' s expectancy beliefs was predicted by authoritarian and behavior encouragement(β=0.076,t =2.35,P=0.019 ;β =0.111,t =3.748,P =0.000).Children' s value beliefs was predicted by affect -warmed and behavior encouragement (β =0.070,t =2.883,P =0.004 ;β =0.070,t =2.745,P =0.006).Children' s sport behavior was predicted by trust-encouragement(β=0.294,t=3.193,P=0.001).The prediction of expectancy beliefs was significant positive by the interaction between indulgence and time involvement (β=0.078,t =2.065,P =0.039).The prediction of expectancy beliefs was significant negative by the interaction between authoritarian and time involvement(β=-0.084,t =-2.454,P=0.014).The prediction of expectancy beliefs was borderline significant positive by the interaction between neglecting and equipment provision(β=0.073,t =1.94,P =0.053).Conclusion Different parenting styles are significant predicators of children sport involvement.Children' s perceptions quality of parental involvement play an important role on parenting style and children' s sport expectancy-value beliefs.

In mice, adriamycin (l7.5mg/kg, ip ) produced heart peroxida-tion abnormality including increased TBA value & decreased gluta-thione peroxidase activity, respectively. But the change of former was not parallel to the latter. The drug also produced early pathological change of the heart i.e. more D-PAS positiveness. The results suggested that the early heart pathological change produced by adriamycin may be related to increased accumulation of pero-xi dative products.

Extensive use of tributyltin (TBT) has caused serious environmental pollution, which is harmful to human and other organisms. As a cytotoxic chemical, an important mode for TBT effects on the cells is to induce apoptosis. The present paper mainly reviewed the mechanism by which TBT induces apoptosis at low concentration.

Objective: To assess the status of the interpersonal relationship of the current college students.Method: Approximately 456 students were tested with the interpersonal relationship of the current college students questionary.Result: In factor scores of associate attitude there existed the significant differences between south and north,low grade and high grade.In campus interpersonal atmosphere,the significant differences existed between students of poverty and students of impoverty.In the teacher and student relationship the significant differences existed between low grade and high grade,students of poverty and students of impoverty,singleton and insingleton.Conclusion: The interpersonal relationship of the college students is very important to students and the psychological education of interpersonal relationship should be conducted.

Objective To investigate the inter-arm invasive blood pressure differences during hip replacement in patients with lateral position and its relationship with position.Methods Sixty-six patients having underwent hip replacement in orthopedic surgery with lateral position were enrolled.Invasive blood pressure measurements were obtained from bilateral radial artery in all patients every 5 minutes.Simultaneously bilateral invasive blood pressure measurements were obtained and compared.Results At supine position,systolic pressure,diastolic pressure and mean arterial pressure of the uninjured side were (128.16 ±20.09),(69.47 ± 8.64) and (89.03 ± 10.42) mmHg (1 mmHg =0.133 kPa);systolic pressure,diastolic pressure and mean arterial pressure of the injured side were (127.90 ± 20.44),(69.86 ± 8.81) and (89.21 ± 10.71) mmHg.There was statistical difference in inter-arm diastolic pressure (P ＜ 0.05),but inter-arm systolic pressure and mean arterial pressure had no statistical differences (P ＞ 0.05).At left-lateral position,systolic pressure,diastolic pressure and mean arterial pressure of the uninjured side were (129.12 ± 21.93),(69.23 ± 8.92) and (89.19 ± 11.35) mmHg;systolic pressure,diastolic pressure and mean arterial pressure of the injured side were (129.01 ± 21.72),(69.51 ± 9.02) and (89.34 ± 11.35) mmHg.There was statistical difference in inter-arm diastolic pressure (P ＜ 0.05),but inter-arm systolic pressure and mean arterial pressure had no statistical differences (P ＞ 0.05).At right-lateral position,systolic pressure,diastolic pressure and mean arterial pressure of the uninjured side were (128.78 ± 20.34),(69.54 ± 9.06) and (89.29 ± 10.82) mmHg;systolic pressure,diastolic pressure and mean arterial pressure of the injured side were (128.27 ± 20.56),(70.00 ± 9.09) and (89.43 ± 10.89) mmHg.There were statistical differences in inter-arm systolic pressure and diastolic pressure (P ＜ 0.05),but inter-arm mean arterial pressure had no statistical difference (P ＞ 0.05).The bilateral radial artery blood pressure difference under different positions was ＜ 1 mmHg,and had no clinical practical significance.There was no correlation between blood pressure and age,sex,body mass index.Conclusion Bilateral radial artery in the same patients with invasive blood pressure has no clinical difference,and patients with lateral position operation for radial artery puncture pressure need not consider the differences of blood pressure between arms.

Objectives To provide outline for the Research & Development department of hospitals making research strategy by analyzing the quantity of publications and their cited status for the top 20 hospitals in SCI and MEDLINE, and the top 100 hospitals in domestic publications in 2008. Methods The number of publications and their cited times for the first 20 hospitals in SCI and MEDLINE, and the top 100hospitals in domestic publications in 2008 were analyzed with bibliometric analysis. Results Compared with 2007, the increased number and ratio of publications in SCI and MEDLINE for the top 20 hospitals were 1050, 51.19% and 658, 15.04%, respectively; for top 100 hospitals, the average decreased number of domestic publications was 2.08, and the number of cited publications was increased 202.52 and the increased ratio was 13.51%. Conclusions The SCI publications from top 20 hospitals increase sharply, the increase of the MEDLINE articles from the top 20 hospitals is slowly. The negative growth is showed in the number of domestic articles for the top 100 hospitals. In addition, the increase of cited articles is slowly and disproportion.

Objective To investigate the information ability of medical personnel from a large general hospital that integrates medical care,teaching and research under the network environment for providing evidence to improve targeted information service in library.Method A self-designed and self-administered questionnaire was distributed through the questionnaire system on hospital office network.Information ability involved in the questionnaire mainly included the ability to precisely retrieve literature in Chinese and foreign language,commonly used retrieval strategies,and screening,evaluation and English full-text access methods.A total of 1 094 medical personnel voluntarily participated and completed the questionnaire,and complete responses were obtained from 1 065 individuals (effective response rate 97.35％).x2 and trend x2 tests were used for data analysis.Results The overall accuracy of precise retrieval information was only 6.1％,and the accuracies of precise retrieval literature in Chinese and foreign language databases were 14.6％ and 14.6％,respectively.Duty,education and working years of the medical personnel had significantly effects on the ability to precisely retrieve literature and the strategies and methods of information retrieval,screening,evaluation and full-text access.Conclusion Medical personnel had certain information ability,but library should carry out relevant training program to improve the medical personnel's information quality and ability further.

The paper firstly introduces the research status of autism and analyzes the gap between China and the US in autism net-work service, proposes using the mainstream socialized Q&A community data in foreign countries to construct an autism Q&A knowledge base.It introduces data collection, management, service process and key issues of the knowledge base and gives application examples of the knowledge services.Construction of this knowledge base can provide targeted and valuable knowledge services to users and can help enhance the retrieval efficiency.