Human metapneumovirus (HMPV)is a recently discovered virus associated with respiratory tract infection. Most infections with HMPV occurred during winter and spring. Children, elderly subjects and immunosuppressed hostsare susceptible. The consequences of HMPV infections ranged from mild upper respiratory tract infection to severe bronchiolitis or pneumonia. There are three different kinds of methods for the diagnosis of HMPV infections, detection of virus antigen-antibody, virus isolation by culture, and RNA detection by reverse transcription-PCR. Symptomatic therapy is the main method of treatment.

0.05).Conclusion:The diabetes itself does not affect the sulfonylurea receptor(SUR2)expression in myocardial tissues.Sulfonylureas at treatment dosage have no effect on receptor expression of SUR2.

Objective To investigate the effect of cerebroprotein hydrolysate on the serum high sensitivity C reactive protein (hsCRP), insulin-like growth factor-1 (IGF-1) and interleukin-18 (IL-18) in patients with hypoxic ischemic encephalopathy (HIE) in plateau region. Methods From January, 2013 to May, 2015, 104 cases with neonatal HIE in our department were randomly divided into conventional treat-ment group (n=52) and cerebroprotein hydrolysate group (n=52). Besides, 35 cases of healthy newborn were chosen as the control group. The total effective rate and the serum hsCRP, IGF-1, IL-18 levels were compared. Results The IGF-1 level was lower, and the levels of hsCRP and IL-18 were higher in moderate HIE group and severe HIE group than in the control group and the mild HIE group, respectively (P<0.05). The efficiency rate was significantly higher in the cerebroprotein hydrolysate group than in the conventional treatment group (χ2=8.922, P=0.012). Compared with the conventional treatment group, the IGF-1 level increased, and the levels of hsCRP and IL-18 decreased in the cerebroprotein hydrolysate group (P<0.05). Conclusion Cerebroprotein hydrolysate is effective on patients with HIE in plateau re-gion. The changes of serum hsCRP, IGF-1, and IL-18 levels can be used as auxiliary indexes for early diagnosis of HIE.

Objective To investigate the value of acoustic radiation force impulse (ARFI) in differential diagnosis between benign and malignant breast tumors.Methods Virtual touch tissue imaging (VTI) and virtual touch tissue quantification (VTQ) of ARFI elastography were used to estimate the properties and extent and measured the shear wave velocities (SWV) of the breast tumors,respectively.Results The mean SWV of breast cancer and benign lesion were (7.84 ±2.51)m/s and (2.79 ±0.92)m/s.There was significant difference in the SWV between benign and malignant breast tumors (P ＜ 0.01).The area under curve (AUC) of receiver operating characteristic (ROC) curve of the SWV in differentiating benign from malignant breast tumors was 0.886.With SWV 4.4 as cut-off,the sensitivity,specificity,accuracy,positive predictive value and negative predictive value in the diagnosis of malignant from benign breast tumors were 78.7％,100％,91.7％,100％,88％,respectively.Conclusions ARFI elastography could reflect the elastic characteristics of breast tumors,which might be useful for the differential diagnosis between benign and malignant breast tumors.

Objective To study the changes of adriamycin-induced glomerular epithelial cells (GECs) permeability and cytoskeleton, and to explore the role and possible mechanism of epithelia growth factor (EGF) on adriamycin-induced glomerular epithelial barrier function. Methods Rat GECs on Milicell-PCF Inserts were exposed to adriamycin (0.5 ?mmol/L) 24 hours in the absence or presence of EGF. The paracelluar permeability to BSA and cell viability were evaluated. The expression of F-actin and alpha-actinin were analyzed by immunofluorescence and Leica laser scan confocal microscopy. Results After induced by adriamycin for 24 hours, paracelluar permeability to BSA and F-actin reorganization rate increased. Disassembling of cortical cytoskeleton architecture was observed. Stress fiber in cytoplasm disappeared. Alpha-actinin staining showed perinuclear enhancement, which is different from normal cytosolic pattern. EGF significantly reduced adriamycin induced effect. Higher level of BSA passed through GEC monolayer in a dose-dependent manner. EGF prevented adriamycin-induced cytoskeletal reorganization. Disassembled cortical cytoskeleton was recovered, and stress fiber appeared again. Alpha-actinin staining mainly exhibited cytosolic uniform distribution as control GEC. In addition, administration of either AG1478, a specific inhibitor of EGF-receptor tyrosine kinase, or U73122, a specific inhibitor of PLC? before EGF treatment attenuated the EGF-mediated effect, whereas neither AG1478 nor U73122 exerted influence in the absence of EGF. Conclusions Adriamycin increases GEC paracellular permeability to BSA as a result of adriamycin-induced cytoskeleton disassembling and disruption. EGF may prevent adriamycininduced cytoskeleton reorganization and maintain glomerular epithelial barrier function through EGF mediated EGF-EGFR-PLC? signal pathway.

This paper reviews the research progress in relations between indoor air pollution and human health for the last 20 years,and focuses on: the studies on environmental risk assessment methods; the major health effects caused by indoor air pollution; the minor and limited health effects caused by indoor air pollution; other key issues concerned.

Objective To summarize the progress of clinical research on triple-negative breast cancer(TNBC).Methods Domestic and international publications on the study of TNBC in recent years were collected and reviewed.Results The patients with TNBC were younger,and their prognosis was poorer.Besides operation,chemotherapy was the major therapeutic tool for them.Currently the targeted therapy for epidermal growth factor receptor and its signal conducting system was applied to clinical therapy gradually,and it might benefit the patients with TNBC.Conclusion The study on TNBC may bring a new way for therapy.

Thiamin and riboflavin content of 15 varieties of rice processed in Guandong province in 3 different milling degrees (unpolished, standard and top grade) were estimated fluorospectrophotometrically. The thiamin content was averaged 0.48?0.01mg/100g for unpolished rice,0.33?0.07mg/100g for standard second grade and 0.16?0.04 mg/100g for top grade rice. The riboflivin content was averaged 0.09 ?0.02mg/100g for unpolished, 0.07?0.01mg/100g for standard second grade and 0.05 ?0.01mg/100g for top grade rice. Rice from Cing Er Ai, Min Ke Zhati, Qi Gui Zhao, Za You, and Zhong Gui rich in thiamin and the mixed third class rice from Shangshui county was rich in riboflaflavin. Rice from both Zhong Gui and Qi Gui Zhao was rich in thiamin and riboflivin. The thiamin and riboflavin content in rice depended on the rice variety and the area where rice was grown. The thiamin content in rice from Nan Hai County was higher than that from Shangshui and Guao Yiao Counties.Certain amount of thiamin and riboflavin was lost in the same kind of rice with diffeererit millig degrees. As compared with unpolished rice, the standard second grade rice lost about 30.63% of thiamin, 22.36% of riboflavin and the top grade rice lost 65.35% and 45.00% respectively.There was some thiamin and riboflavin loss during different storage periods, 4.85% of thiamin, 4.23% of riboflavin were lost after 6 months storage, and 10.3% of thiamin, 7.04% of riboflavin lost after one year storage.In order to improve the nutrition status of the people, it is necessary to advocate people to consume fresh and standard grade rice and to encourage cultivators to grow crops rich in nutrients.

The possible mechanism of insulin resistance of the adipose tissue after burn injury was studied.The binding activity of the insulin receptors,the insulin effects on insulin-sensitive phosphodiesterase(PDE)and the level of free fatty acid(FFA)and cAMP were observed for 4 d in the adipose tissue of the rats inflicted with 30% TBSA full thickness scalding. It was found that the maximal binding capacity of the insulin receptors was greatly decreased(P

Intralipid is a kind of important fat emulsion.It has been widely used clinically in severely ill patients,especially in the patients before and or after major operation since the 1980s.After retrieving the papers on Intralipid form MEDLINE CD ROM database from 1980 to 1994 and analysing them by linear regression analysis in literature metrology,We found a significant linear correlation between the yearly literature quantity and the dynamic time course (year).From 1980 to 1994,the average yearly growth rate of the literature was 1.16.According to the regression equation,it is predicted that the literature on Intralipid will increase at a rate of 2.07% per year.From this analysis,we think that the research of Intralipid is tending to stability and will be wonderful.