Aim To investigate the effects of paeonol on lipopolysaccharide ( LPS) and adenosine 5′-triphos-phate ( ATP) induced NLRP3 inflammasome activation in primary rat microglia and the mechanisms responsi-ble for this anti-inflammatory effects. Methods Pri-mary rat microglia were identified immunohistochemi-cally using the cluster of differentiation 11 b ( CD11 b ) antibody. Proinflammatory cytokine IL-1β was deter-mined by ELISA. Western blot was performed to ob-serve the protein expression of NLRP3 , ASC and caspase-1 in cultured primary rat microglia. The level of intracellular reactive oxygen species ( ROS) was mo-nitored by using the fluorescent probe 2′, 7′-dichlo-rofluorescein diacetate ( DCFH-DA ) . Results LPS (0. 5 mg · L-1 )/ATP ( 5 mmol · L-1 ) significantly increased intracellular ROS level and IL-1β secretion and upregulated NLRP3 , ASC and caspase-1 protein expression in primary rat microglia. Paeonol signifi-cantly decreased intracellular ROS level and IL-1β se-cretion, and inhibited LPS/ATP induced overexpres-sion of NLRP3 , ASC and caspase-1 in cultured primary rat microglia. Conclusion Paeonol can inhibit LPS/ATP induced NLRP3 inflammasome activation in pri-mary rat microglia, and this inhibitory effect may be through the suppression of intracellular ROS.

Objective To observe the changes of combined therapy on disci intervertebrales and their surrounding tissues and thus to determine its therapeutic effects on lubar intervertebral disc protrusion with magnafic resonance imaging. Methods After combined therapy including physical agents, drugs, traction, manipulation and remedial exercises had been adopted to treat 40 iubax intervertebral disc protrusion patients for 30 days, morphologic changes of disci intervertebrales and their surrounding tissues of these patients were observed by magnetic resonance imaging. Results The effective rate of combined therapy on lubar intervertebral disc protrusion was 82.5%, there were no obvious changes of thickness, salient degree of disci intervertebrales, anteroposterior diameter, transverse diameter and latero-cryptae extent of vertebral canal after the treatment. Conclusion Though the combined therapy can not change the morphous of lobar intervertebral disc protrusion, it may efficiently relief the clinical symptoms of patients with lobar intervertebral disc protrusion.

The main teaching methods for medical students and residents are didactic teaching,rote learning and apprenticeship.Although these teaching methods can be good for imparting knowledge,they are obviously insufficient for the training of technical skills,team cooperation and complex decision making.Application of biomedical engineering simulation in the anesthesia teaching can partially compensate for the insufficiency.The progress of biomedical engineering simulation in anesthesia teaching is reviewed and the educational trend was explored.

Objective To evaluate a new suture coil in endoscopic submucosal dissection (ESD). Methods Data of 15 patients with early gastrointestinal cancer undergoing ESD were retrospectively ana?lyzed. The edge of the lesion was pulled up by the new coil?assisted suture for the submucosal layer to be full?y revealed, then the submucosa were dissected,and the wound was stitched by suture coil. The success rate and complications were evaluated. Results The lesions were successfully resected in 15 patients. By pull?up, the submucosal blood vessels were completely revealed. No intraoperative uncontrollable bleeding oc?curred. Small perforation occurred in one patient, and was well stitched by the suture coil. The pull?ups were successfully completed in all patients, and the operation time was 5?10 min. The intraoperative slippage of fixed titanium clips occurred in two cases. All the wounds were stitched. Minor bleeding happened in 1 pa?tient, and was controlled by hemostatic clip. Conclusion The new suture coil is easy to use,and can effec?tively prevent and deal with the occurrence of intraoperative and postoperative ESD?related complications.

Retrospective analyses were conducted for the clinical data of two cases with pseudoMeigs' syndrome at our hospital.Both had respiratory symptoms,such as cough and dyspnea.Radiological examinations revealed ascites,pleural effusion and pelvic mass.The definite pathological diagnosis was pelvic malignant tumor.After surgical tumor removal,ascites and pleural effusion disappeared without recurrence.Along with reviewed cases from PubMed in the last decade,a total of 49 cases had pseudoMeigs' syndrome.The age range was 11-73 years.Their clinical manifestations include dyspnea (78％),abdominal distension (69％),cough (14％),abdominal pain (12％),fatigue (10％),weight loss (6％) chest pain (4％),fever (4％),abdominal mass (4％),and oliguria (2％).CA125 was commonly elevated.The primary tumors in pelvic cavity accounted for 78％.And ovarian metastasis from colon cancer was one of the most common in metastatic tumor.

Antibodies, Antiphospholipid ; blood ; immunology ; Antiphospholipid Syndrome ; diagnosis ; etiology ; immunology ; Child, Preschool ; Diagnosis, Differential ; Humans ; Male ; Partial Thromboplastin Time

Adult ; Aged ; Female ; Femur ; injuries ; Humans ; Male ; Manipulation, Orthopedic ; methods ; Middle Aged ; Patellar Dislocation ; diagnostic imaging ; pathology ; physiopathology ; therapy ; Radiography

AIM: To investigate the inhibiting effect and mechanism of dimethylaminoethyl ginkgolide B mesylate on platelet aggregation and release function.METHODS: The effect of dimethylaminoethyl ginkgolide B mesylate on inhibiting PAF-induced platelet aggregation was measured by turbidimetry method through giving rabbits dimethylaminoethyl ginkgolide B mesylate at different final concentration via i.v.for 5 days.The release of Ca2 + from PAF-induced platelet in rabbits was assayed with fluorospectrophotometry and the contents of TXA2 and PGI2 were measured by radio-immunity method.RESULTS: Three groups of dimethylaminoethyl ginkgolide B mesylate (1.95,3.90,7.80 mg/kg) had significant effect on inhibiting PAF-induced platelet aggregation in rabbits (compared to normal,P

AIM: To investigate the influence of Astragalus Injection on morphology,cell cycle and oxidative stress of rat's glomerular mesangial cells(GMC) cultured with advanced glycation end products(AGEs). METHODS: GMC were incubated in culture medium containing AGEs in the presence of Astragalus Injection and aminoguanidin for 48 h.At the same time,the normal and control groups were established.Then the cultured GMC was stained by mixed fluorescence liquid and observed under fluorescence microscope.Cell cycle of GMC was analyzed using flowcytometry.The activity of SOD、MDA and GSH-PX in GMC supernatant were measured by test kit.The level of ROS was detected by flowcytometry. RESULTS: Morphology analysis showed that the morphology and structure of normal GMC were normal.The structure of most cells in AGEs was unclear,cell counts increased markedly and they grew intensively.Cell cycle analysis showed that cell percentage of S phase increased and G_0/G_1 reduced.The level of ROS,MDA remarkably increased,and SOD,GSHPX activity reduced.Whereas Astragalus Injection was added,cell morphology tended to be basically normal and cell counts decreased,the percentage of S phase also decreased.The level of ROS,MDA and the SOD,GSH-PX activity restored in comparison with the control groups. CONCLUSION: Astragalus Injection can prevent GMC from lesion caused by AGEs.Astragalus Injection may protect GMC from retarding the progression of diabetic nepropathy by partially inhibiting the occurrence of oxidative stress.

Objective To investigate the protective effects of 1-hydroxy-2, 3, 5-trimethoxyxanthone (QGS) on acute lung injury of mice induced by ip lipopolysaccharide (LPS). Methods Mice were pretreated with QGS for 7 d. Murine models of acute lung injury were duplicated by injection of LPS 20 mg/kg intraperitoneally. In 12 h, the lung weight index was observed and the NO level in the bronchoalveolar lavage fluid (BALF) was measured with kits. The lung was also assessd for the expression of I-?B, inducible nitric oxide synthase (iNOS), and cyclooxygenase-Ⅱ (COX-2) using Western blotting analysis. Lung pathological changes were also observed by HE in each group. Results The lung weight index of injury lung in mice induced by LPS was decreased in 500 mg/kg QGS group (P