PURPOSE To sutdy the effects of dibutyl pathalate (DBP) on the proliferation and apoptosis of leukemic cells from patients suffering from acute leukemia. METHODS The cell culture in vitro was used to study the effect of DBP on the proliferation of leukemic cells, while agrose gel electrophoresis of DNA fragments was used to study the effect of DBP on apoptosis of leukemic cells. RESULTS DBP could inhibit the proliferation of leukemic cells got from patients suffering from acute leukemia in vitro. The percentage of inhibition were 24.24% and 36.33% respectively when leukemic cells were exposed to 50?g/ml and 200?g/ml DBP for 24 hours. Leukemic cells from acute myelogenous leukemia were more susceptive to DBP than those from acute lymphocytic leukemia. DBP also induced the leukemic cells from patients to die via apoptosis and showed typical "ladder" of DNA fragments for apoptotic cells in agrose gel electrophoresis. CONCLUSION DBP could clear the leukemic cells in bone marrows of patients suffering from acute leukemia by suppressing the growth of leukemic cells and inducing them to die via apoptosis.

OBJECTIVE:To discuss the research direction of the database of traditional Chinese drugs and to facilitate the modernization of the traditional chinese medicine information.METHODS:The current domestic traditional chinese medicine databases are classified and sorted systematically and the problems occurred in the database construction were dis-cussed.RESULTS&CONCLUSION:The developmental tendency of traditional chinese medicine database is characterized by setting up and evolving the research&development(R&D)information system of traditional chinese medicine,improving the shared platform of traditional chinese medicine database established in the internet,and establishing and improving the tradi-tional chinese medicine market information system,etc.

Objective To study the effects of letrozole and clomiphene on the treatment of ovulation induction and the changes of reproductive hormone in the patients with polycystic ovary syndrome.Methods In Zhenhai District People's Hospital from January 2015 to December 2016, 100 cases with polycystic ovary syndrome as the research object in this study, were randomly divided into the control group and the experimental group, 50 cases in each group.The control group were treated with clomiphene, the experimental group were given letrozole combined clomiphene.The levels of reproductive hormones, ovulation rate and pregnancy rate were compared in the two groups.Results The ovulation rate was 90%, pregnancy rate was 46%, the numbers of follicles was(1.2±0.4).In the control group, the ovulation rate was 72%, the pregnancy rate was 22%, and the number of follicles was(2.2±0.5).The results showed that the ovulation rate and pregnancy rate in the experimental group were significantly higher than those in the control group, with statistical significance(P<0.05).The number of follicles in the control group was significantly higher than that in the control group, with statistical significance(P<0.05).There was no significant difference between the experimental group and the control group in the time of follicular maturation, which was not statistically significant.The levels of reproductive hormones in the experimental group were significantly lower than those in the control group, with statistical significance(P<0.05).Conclusion Letrozole combined clomiphene can improve the ovulation rate and pregnancy rate, reduce the reproductive hormone level, which has clinical application value.

Objective To investigate the effect of Daying-35 combined with metformin on ovulation induction after polycystic ovary syndrome, and to summarize the clinical experience.Methods Forty patients with polycystic ovary syndrome were treated with Daying-35 and treated as control group.Forty-five patients were treated with meta-35 combined with metformin and treated as observation group.After treatment, Level and ovulation induction, selected patients were our hospital in January 2016 to December 2016 between the treatment.Results There were no significant differences in T, FSH, LH, E2 and other hormone levels between the two groups before treatment.After treatment, the improvement of the treatment group was significantly more obvious;compared with the two groups of ovarian hyperstimulation syndrome and The rate of pregnancy was 47.5% in the observation group and 15.0% in the control group.The difference between the two groups was significant(P<0.05).Conclusion The effect of Daying-35 combined with metformin on ovulation induction after polycystic ovary syndrome was found to improve the progesterone level and improve the clinical status of the patients.Making the ovulation situation improved, improve the pregnancy rate, it is worth clinical reference.

Physical constitution theory is an important part of traditional Chinese medicine theory. Physical constitution is associated with the incidence, development and prognosis of diseases. Gene polymorphism is a result of the interaction between internal and external environments during the human evolution, and it is the reason for differences in biological characteristics and disease susceptibility of individuals. Current status of the research on physical constitution theory, lung cancer syndromes and gene polymorphism are summarized in this paper. Exploring lung cancer syndromes from the point of view of gene polymorphism may reveal the scientific connotation of lung cancer syndromes, and provide new evidence and method for diagnosis of lung cancer.

AlM: To observe the influence ofphacoemulsification on corneal endothelial cell in patients with the different level of HbA1c.METHODS:With case-control study, 164 eyes from 115 cataract patients were divided into four groups according to the preoperative HbA1c level. Control group A: 43 eyes from 30 cases without diabetes ( HbA1c≤6. 5%). Diabetes groups:group B was consisted of 38 eyes from 26 cases(HbA1c≤6. 5%), 40 eyes of 28 case was in group C (6. 5% 8. 0%). Corneal endothelial microscopy was used to detect the parameters like endothelial cell density ( CD ) , coefficient of variation ( CV ) , percentage of regular hexagonal cells (6A) between pre-operation and post-operation at 1d, 1, 2wk, 1mo. The results were analyzed.RESULTS: The endothelial cell density, CV and 6A cell had no significant difference in four groups before treatment (P>0. 05). The CD and 6A were decreased and CV was increased in all groups after operation of 1d, 1, 2wk and 1mo. The difference was statistically significant (P< 0. 05). There were statistical differences of 6A and CV in diabetes groups compared with control group ( P<0. 05). The decreasing of CD between group A and group B had no difference (P>0. 05). There were statistical differences of CD in group C and group D compared with group A (P<0. 05). There were statistical differences of CD, 6A and CV within diabetes groups (P<0. 05). CONCLUSlON: Cataract phacoemulsification had certain degree damage on corneal endothelial cell. Corneal endothelial appeared the minimal injury when the postoperative HbA1c≤6. 5%. For diabetic cataract patients, the higher HbA1c before, the heavier damaged after the surgery.

Objective To establish a cell line of human ovarian cancer, and study its characterization. Methods The cell line was established by the cultivation of subsides walls, and kept by freezing. The morphology was observed by microscope and electromicroscope. The authors studied its growth and propagation, the agglutination test of phytohemagglutinin (PHA), the chromosome analysis, heterotransplanting, immuno histochemistry staining, the analysis of hormone, the pollution examination and the test of sensitivity to virus etc. Results A new human ovarian carcinoma cell line, designated ovarian mucinous cystadenocarcinoma 685 (OMC685), was established from mucinous cystadenocarcinoma. This cell line had subcultured to 91 generations, and some had been frozen for 8 years and revived, still grew well. This cell line possessed the feature of glandular epithelium cancer cell. The cells grew exuberantly, and the agglutinating test of PHA was positive. Karyotype was subtriploid with distortion. Heterotransplantations,alcian blue periobic acid schiff (AbPAS),mucicarmine,alcian blue stainings, estradiol (E 2) and progesterone were all positive. Without being polluted, it was sensitive to poliovirus Ⅰ, adenovirus 7 and measles virus. Conclusions OMC685 is a distinct human ovarian tumous cell line.

Objective: To research identification methods of the two crude drugs from the original plants with close relationship and very similar macroscopic shape,"Chuipencao"(Herba Sedi) and "Fujiacao"(Herba Sedi Linearis).Methods:Microscopic technique and TLC were used to determine the pharmacognostical characters for identification purpose.Results: The microscopic observations showed that the tissue structures of the stems and the microscopic characters of the powders were very similar between the two herbal drugs.But there were significant differences in number of the vessel,shape of the pith,and number of the purplish-red cell.For the samples collected in August,number of the vessel of the former usually was only 35-58,while the latter was 75-150.Pith of the former usually was in the shape of a triangle,while the latter was in the shape of Y.The TLC result had also obvious difference between the two drugs.Besides,there were some differences of the pharmacognostical characters of the samples collected in distinct periods.Conclusion: The results provided scientific basis for the identification of the two crude drugs and suggested that the general microscopic features be not important in the microscopic identification of the crude drugs from the original plants with closer relationship.

20% lymphocytes)was documented in all patients who underwent bronchoalveolar lavage(BAL).In addition,inversion of the CD4/CD8 ratio(mean value:0.64?0.21)was observed in the patients.Non-specific IgM and IgG antibodies to antigens were documented in 13/14(92.9%)of BAL patients.Ten patients were cured by glucocorticoid,others by anti-infective therapy.We rechecked the HRCT which displayed the shade of lung in 12 patients(85.7%)vanishing completely,2 patients'(14.3%)partly.Conclusion There is no specificity in the clinical manifestations,change of mageology,pulmonary function and the immunologic test.However,compared with other methods,we find the test of BAL is more valuable to the diagnosis.It is critical to avoid the antigen,and we find the curative effect of the glucocorticoid is significant.

Objective: To explore the characteristics of IR spectra of the crude drug Herba Sedi collected in different habitats and seasons.Methods: The infrared spectra of all the samples of the whole plants were obtained by FT-IR spectroscopy,the sample powder tablets were used,and identifi cation features were determined through comparative study.Results: The harvest seasons of the samples are remarkly related to the positions(cm-1),intensity or ratios of some major absorbing peaks of the infrared spectra from the 15 samples of several different months and 4 habitats in Hubei Province,China.As for peak positions,the peak values(1625?7)cm-1 of all the samples in spring are less than 1623cm-1,but in autumn(April to October) are generally more than 1628 cm-1.And peak values(1057?16)cm-1 of the samples of spring are more than 1050 cm-1,but less than 1050 cm-1 for the samples of summer and autumn.Moreover,the habitats of samples are also related to IR spectra to some certain extent.Conclusion: The harvest seasons of Herba Sedi from Hubei Province can be preliminarily ascertained according to the IR spectra features.IR technique should be paid attention to in research and identifi cation of the harvest periods of crude plant drugs.