Objective To evaluate the value of ultrasound and CT in the diagnosis of adrenal lesions .Methods A total of 70 pa‐tients of adrenal lesions were examined by ultrasound combined with CT .Pathologically confirmed 55 cases of adrenal tumors and 2 of cysts ,13 of hematoma with follow‐up .The results were analyzed and compared with ultrasound or CT individual inspection .Re‐sults The diagnosis accuracy of adrenal lesions by ultrasound with CT ,only ultrasound and CT ,were 88 .6% ,62 .8% and 72 .9% , 14 pheochromocytoma ,13 myelolipoma ,including 15 adenoma ,5 cortical carcinoma ,2 NHL ,6 metastalic tumor ,2 cysts ,13 hemato‐ma .There was a significant difference between two methods (P<0 .05) .Conclusion The combination of ultrasound and CT could improve accuracy greatly in diagnosis of adrenal lesions .

The non-invasive positive-pressure ventilation(NPPV) play an important role in treating patient with upper airway obstructive diseases.The use of NPPV in children has significantly increased in recent years internationally.Nevertheless,the paediatric NPPV support in China was less reported.This review aimed to clarify the indications,efficacy and technical methods in NPPV,and therefore searched papers including clinical studies,reviews and guidelines focusing on children with upper airway obstructive diseases who were treated on NPPV.The review concluded that NPPV is a treatment choice for patient who has upper airway obstruction and can not be solved by surgery.The NPPV avoids the impairment to the body caused by recurrent hypoxemia and arousals,decreases the rate of intubation and improves the quality of life.NPPV need to be preformed in a professional children's medical center.Education and training for the parent is the key point of improving treatment compliance.

Objective To construct interpretation theory which is suitable to explain the connotation of interpersonal relationship among associate degree nursing students.Methods The data were collected through deep interviews.The content of interpersonal relationship was affirmed by three-level coding and comparing repeatedly.Results The core concept was gaining a feeling of belonging and it could be affected by 5 generic concepts including clinical teachers,head nurses,baccalaureate degree nursing students,patients and doctors.Conclusions Harmonious interpersonal relationship can be built according to the theory of interpretation and practice outcomes can be improved.

Objective To investigate the role of platelet-derived growth factor-β receptor (PDGFR-β) in self-renewal of neural stem cells (NSCs).Methods In this study,NSCs of subventricular zone were isolated and cultured from PDGFR-β knockout (PDGFR-β-/-) mice of postnatal day 1 (P1) and P28;the number and diameters of secondary neurospheres were calculated;using 5-bromo-2-deoxyuridine (BrdU) incorporation assay and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay,cell proliferation and survival rates were analyzed;gene expression profiles were determined by PCR-array analyses;the effect of brain-derived neurotrophic factor (BDNF) on secondary neurospheres formation was examined in these cells.Results In PDGFR-β-/-NSCs,stem cell activities,such as number (/well;P1:25.9±1.3vs117.6±3.6,t=4.236,P＜0.01;P28:13.8± 0.7vs 19.8±0.6,t=2.116,P＜0.01)and diameters (μm;P1:67.7±1.9 vs 69.1 ±2.0,t=3.211,P＜0.01;P28:33.4±0.8vs37.8±0.8,t=2.354,P ＜0.01) of secondary neurospheres,cell proliferation (％;P1:73.3 ± 2.7 vs 88.7 ± 3.6,t =2.773,P ＜ 0.05;P28:28.6 ± 9.6 vs 68.2 ± 4.5,t =6.302,P ＜ 0.05) and survival rates (％;P1:14.5 ±2.1 vs 9.3 ± 1.3,t =7.222,P ＜ 0.05),were significantly lower as compared with age-matched controls.In comparison of the same genotypic NSCs,the decrease of secondary neurosphere formation was more striking in P28 NSCs than in P1 NSCs.PCR Array analyses demonstrated that expressions of fibroblast growth factor2 and BDNF were decreased (-2.04 ± 0.25,t =2.653,P ＜ 0.05;-3.24 ± 0.37,t =1.324,P ＜ 0.05),and Noggin (2.31 ± 0.37,t =2.749,P ＜ 0.05) was increased in P1 PDGFR-β-/-NSCs as compared with P1 controls.Addition of BDNF rescued the number and diameter of secondary neurospheres in P1 PDGFR-β-/-NSCs to similar levels as controls.Conclusions PDGFR-β signaling may play a role in the selfrenewal and proliferation of NSCs.BDNF may be involved in the effects of PDGFR-β signaling in these cells.

0.05). Conclusion The present method has many advantages such as simplicity of operation,rapidity,better accuracy for the accurate quantitative analysis of urinary manganese.

A method for the digestion of food samples by using PTFE closed-vessel microwave oven has been established. Six elements in the solution were determined by ICP-AES. The effects of various acid mixtures and digestion time were studied. 0.2 g of powdered samples dried for four hours at 80℃ were weighed and put into 50 ml PTFE digestion vessels. 2 ml of 70% HNO3 and 1 ml of 30% H2O2 were added to each sample digestion vessel. The screw caps were tightened and placed on the microwave oven PTFE turntable for 3 min at 100% full power (500W). When digestion was finished, the vessels were cooled for 10 min in a medical refrigerator at -25℃. The sample solutions were diluted to 25 ml with subboiling water. The contents of Ca, Mg, Mn, Fe, Zn and Cu in 3 standard reference materials were determined by ICP-AES. The results are in good agreement with certified values.

Objective:To study the content of total flavones in different parts of Mosla dianthera harvested in different seasons to pro-vide reference for the determination of optimal medicinal part and harvesting time of the plant. Methods:The total flavones content of M. dianthera was determined by TLC-UV. Results:The content of total flavones extracted from the plant in September was highest, and that in the leaves was much higher than that in the stems or the roots. Conclusion:It is advisable to harvest the aerial parts of M. dianthera in September.

Objective To provide anatomical basis for one-stage full dynamic correction of late facial palsy with double latissimus dorsi segmental muscle flaps.MethodsForty sides of formaldehyde fixed latissimus dorsi in twenty cadavers were selected to microsurgery anatomy,three sides fresh latissimus dorsi specimens were blood vessel casted.The extramusclar and intramuscular neurovascular distribution were observed.Results①In 92.5%of the thoracodorsal nerve divides into a medial and a lateral branch be fore entering the muscle.In 7.5%of the thoracodorsal nerve divides into three major branches.The coordinate of the bifurcation point of the thoracodorsal Here was[(7.94±1.23)cm,(3.71±1.68)cm].In the district of this angle's middle line,the numbers of vessels and nerves is relative few.②The lateral muscle flaps of the latissimus dorsi can be divided into 3-5 independent segmental flaps,the medial muscle flaps of the latissimus dorsi Can be divided into 2-4 independent segmental flaps.③The segmental nerve usually locate the two sides of vessel,which are arranged(from medial to lateral)in a NVAV order in the reedial segmental branches(100%),in a VAVN order in the lateral segmental branches(85.0%)and in a NVAV order in the lateral segmental branches(15.0%).④The neural pedicle Was cut from the lateral bifurcation point,the mean length of the third medial muscle neural pedicle was 16 cm；the mean length of the third or the forth lateral muscle neural pedicle was 12 cm.Conclusion Late facial palsy can be cuwd with onestage cross-facial transplantation of neurovascularized free double latissimus dorsi segmental muscle flaps.

Objective　To study the ultrasound image feature of acute hepatic lesions in different times.Methods　The feature of ultrasonography in 126 cases with acute hepatic lesions were analyzed and divided into two groups according to different fime after injury: group A, 25～60 minutes after injury; group B, 60～120 minutes after injury.Results　There was significant difference in the structure of sonographic classification between two groups. The high echos was obviously higher in group A than that in group B, while the mixed echos was obviously higher in group B than that in group A (P<0.001). Compared each types in group A and in group B,the high achos in group A and the mixed achos in group B were higher than other types in same group (P<0.001).Conclusions　The ultrasonographic feature of acute hepatic lesions would change with time. The high echos occurs in eaily time after injury ,and then changes into the mixed echos.

ObjectiveTo investigate the effect of thalidomide on the proliferation and apoptosis in human pancreatic cancer cell SW1990 in vitro.MethodsSW1990 cell line was treated with thalidomide at different concentrations (3.125,6.25,12.5,25,50,100,200 and 400 μg/ml) for 24,48,72 h,and then cell proliferation were evaluated by MTT.Cell cycle was determined by flow cytometry,apoptosis was determined by annexin V/PI fluos staining,Bcl-2,Bax protein expression and Bcl-2/Bax ratios were measured by Western Blot in vitro.ResultsThalidomide inhibited the proliferation of SW1990 cells in a time and dosedependant manner.The proportion of G0/G1 phase of SW1990 cells with 200 μg/ml thalidomide treatment increased from (41.15 ± 2.23 ) ％ to (58.83 ± 2.33 ) ％,apoptosis rate increased from 2.6％ to 28.0％,the expression of Bax protein up-regulated from 0.17 ± 0.03 to 0.33 ± 0.04,the expression of Bcl-2 protein downregulated from 0.35 ± 0.02 to 0.17± 0.01,the ration of Bcl-2/Bax decreased from 2.17 ± 0.44 to 0.52 ±0.07.ConclusionsThalidomide can inhibit the proliferation of pancreatic cancer SW1990 cells by upregulating Bax,down-regulating Bcl-2,inducing cell apoptosis and cell Go/G1 phase arrest.